| 出品公司: | ATCC |
|---|---|
| 载体名称: | pE194 |
| 产品编号: | 37128 |
| 生长条件： | 30°C 1 day(s) |
| 生物安全等级: | 1级 |
| 宿主菌: | 金黄色葡萄球菌芽孢杆菌 |
| 载体类型: | 质粒 |
| 复制子: | pE194 (S.aureus) |
| 抗性: | 红霉素 |
| 启动子: | |
| 建议宿主菌: | |
| 载体大小（kb）: | 3.7 |
| 多克隆位点: | 否 |
| 备注： |
克隆的限制摘要给出以下大小（kb）：acci-3.8；psti-3.8。温度敏感，’对于完全诱导，质粒需要一段时间通过红霉素的亚抑制浓度诱导。金黄色葡萄球菌天然分离物，’对大环内酯、林考酰胺和B型链霉素“MLS”抗生素产生耐药性。
|
| 参考文献: |
P1
Gryczan T, et al. 1980. Characterization of chimeric plasmid cloning vehicles in Bacillus subtilis J. Bacteriol. 141: 246-253.
P12
Horinouchi, S., and B. Weisblum. 1980. Posttranscriptional modification of mRNA conformation : mechanism that regulates erythromycin-induced resistance. Proc. Natl. Acad. Sci. U. S. A. 77: 7079-7083.
P454
Iordanescu S. 1977. Relationships between cotransducible plasmids in Staphylococcus aureus. J. Bacteriol. 129: 71-75.
P455
Weisblum B, et al. 1979. Plasmid copy number control: isolation and characterization of high-copy-number mutants of plasmid pE194. J. Bacteriol. 137: 635-643.
P456
Horinouchi S, Weisblum B. 1982. Nucleotide sequence and functional map of pE194, a plasmid that specifies inducible resistance to macrolide, lincosamide and streptogramin type B antibiotics. J. Bacteriol. 150: 804-814.
P48
Gryczan, T. J., and D. Dubnau. 1978. Construction and properties of chimeric plasmids in Bacillus subtilis. Proc. Natl. Acad. Sci. U. S. A. 75: 1428-1432.
|
买家导航
pE194载体 ATCC37128质粒基本信息
pE194质粒图谱 ATCC37128载体图谱和pE194载体序列 ATCC37128质粒序列多克隆位点信息
pE194质粒 ATCC37128载体简介
pE194质粒序列 ATCC37128载体序列
LOCUS pE194 3728 bp DNA circular CON 09-JUN-2022
DEFINITION Staphylococcus aureus plasmid pE194, complete sequence.
ACCESSION NC_005908 J01755 J01756 J01757 J01758 V01279 V01280
VERSION NC_005908.1
DBLINK BioProject: PRJNA224116
BioSample: SAMN14229318
KEYWORDS RefSeq; circular; drug resistance gene; methylase; origin of
replication; unidentified reading frame.
SOURCE Staphylococcus aureus
ORGANISM Staphylococcus aureus
Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
Staphylococcus.
REFERENCE 1 (bases 1 to 3728)
AUTHORS Horinouchi,S. and Weisblum,B.
TITLE Nucleotide sequence and functional map of pE194, a plasmid that
specifies inducible resistance to macrolide, lincosamide, and
streptogramin type B antibodies
JOURNAL J Bacteriol 150 (2), 804-814 (1982)
PUBMED 6279574
COMMENT REFSEQ INFORMATION: The reference sequence is identical to
V01278.1.
The annotation was added by the NCBI Prokaryotic Genome Annotation
Pipeline (PGAP). Information about PGAP can be found here:
https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
##Genome-Annotation-Data-START##
Annotation Provider :: NCBI RefSeq
Annotation Date :: 06/09/2022 03:03:41
Annotation Pipeline :: NCBI Prokaryotic Genome
Annotation Pipeline (PGAP)
Annotation Method :: Best-placed reference protein
set; GeneMarkS-2+
Annotation Software revision :: 6.1
Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA;
repeat_region
Genes (total) :: 5
CDSs (total) :: 5
Genes (coding) :: 4
CDSs (with protein) :: 4
Genes (RNA) :: 0
tRNAs :: 0
ncRNAs :: 0
Pseudo Genes (total) :: 1
CDSs (without protein) :: 1
Pseudo Genes (ambiguous residues) :: 0 of 1
Pseudo Genes (frameshifted) :: 1 of 1
Pseudo Genes (incomplete) :: 0 of 1
Pseudo Genes (internal stop) :: 0 of 1
##Genome-Annotation-Data-END##
COMPLETENESS: full length.
FEATURES Location/Qualifiers
source 1..3728
/organism="Staphylococcus aureus"
/mol_type="genomic DNA"
/db_xref="taxon:1280"
/plasmid="pE194"
gene join(3150..3728,1..633)
/locus_tag="HXH62_RS00005"
/old_locus_tag="pE194p1"
CDS join(3150..3728,1..633)
/locus_tag="HXH62_RS00005"
/old_locus_tag="pE194p1"
/inference="COORDINATES: ab initio
prediction:GeneMarkS-2+"
/GO_function="GO:0003677 - DNA binding [Evidence IEA]"
/GO_process="GO:0006310 - DNA recombination [Evidence
IEA]"
/note="Derived by automated computational analysis using
gene prediction method: GeneMarkS-2+."
/codon_start=1
/transl_table=11
/product="MobV family relaxase"
/protein_id="WP_011178348.1"
/translation="MSHSILRVARVKGSSNTNGIQRHNQRENKNYNNKDINHEETYKN
YDLINAQNIKYKDKIDETIDENYSGKRKIRSDAIRHVDGLVTSDKDFFDDLSGEEIER
FFKDSLEFLENEYGKENMLYATVHLDERVPHMHFGFVPLTEDGRLSAKEQLGNKKDFT
QLQDRFNEYVNEKGYELERGTSKEVTEREHKAMDQYKKDTVFHKQELQEVKDELQKAN
KQLQSGIEHMRSTKPFDYENERTGLFSGREETGRKILTADEFERLQETISSAERIVDD
YENIKSTDYYTENQELKKRRESLKEVVNTWKEGYHEKSKEVNKLKRENDSLNEQLNVS
EKFQASTVTLYRAARANFPGFEKGFNRLKEKFFNDSKFERVGQFMDVVQDNVQKVDRK
REKQRTDDLEM"
gene 1011..1178
/locus_tag="HXH62_RS00010"
CDS 1011..1178
/locus_tag="HXH62_RS00010"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_006308237.1"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="cop-6 protein"
/protein_id="WP_006308237.1"
/translation="MVVDRKEEKKVAVTLRLTTEENEILNRIKEKYNISKSDATGILI
KKYAKEEYGAF"
gene 1259..1859
/locus_tag="HXH62_RS00015"
/pseudo
CDS 1259..1859
/locus_tag="HXH62_RS00015"
/inference="COORDINATES: protein motif:HMM:NF013849.2"
/note="frameshifted; Derived by automated computational
analysis using gene prediction method: Protein Homology."
/pseudo
/codon_start=1
/transl_table=11
/product="replication protein"
gene complement(2004..2738)
/gene="erm(C)"
/locus_tag="HXH62_RS00020"
/old_locus_tag="pE194p5"
CDS complement(2004..2738)
/gene="erm(C)"
/locus_tag="HXH62_RS00020"
/old_locus_tag="pE194p5"
/EC_number="2.1.1.184"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_011282421.1"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="23S rRNA (adenine(2058)-N(6))-methyltransferase
Erm(C)"
/protein_id="WP_007410443.1"
/translation="MNEKNIKHSQNFITSKHNIDKIMTNIRLNEHDNIFEIGSGKGHF
TLELVKRCNFVTAIEIDHKLCKTTENKLVDHDNFQVLNKDILQFKFPKNQSYKIYGNI
PYNISTDIIRKIVFDSIANEIYLIVEYGFAKRLLNTKRSLALLLMAEVDISILSMVPR
EYFHPKPKVNSSLIRLSRKKSRISHKDKQKYNYFVMKWVNKEYKKIFTKNQFNNSLKH
AGIDDLNNISFEQFLSLFNSYKLFNK"
gene complement(2798..2857)
/gene="ermCL"
/locus_tag="HXH62_RS00025"
CDS complement(2798..2857)
/gene="ermCL"
/locus_tag="HXH62_RS00025"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_002497446.1"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="23S rRNA methylase leader peptide ErmCL"
/protein_id="WP_002497446.1"
/translation="MGIFSIFVISTVHYQPNKK"
ORIGIN
1 gatcagtaca agaaagatac tgtatttcat aaacaggaac tgcaagaagt taaggatgag
61 ttacagaagg caaataagca gttacagagt ggaatagagc atatgaggtc tacgaaaccc
121 tttgattatg aaaatgagcg tacaggtttg ttctctggac gtgaagagac tggtagaaag
181 atattaactg ctgatgaatt tgaacgcctg caagaaacaa tctcttctgc agaacggatt
241 gttgatgatt acgaaaatat taagagcaca gactattaca cagaaaatca agaattaaaa
301 aaacgtagag agagtttgaa agaagtagtg aatacatgga aagaggggta tcacgaaaaa
361 agtaaagagg ttaataaatt aaagcgagag aatgatagtt tgaatgagca gttgaatgta
421 tcagagaaat ttcaagctag tacagtgact ttatatcgtg ctgcgagggc gaatttccct
481 gggtttgaga aagggtttaa taggcttaaa gagaaattct ttaatgattc caaatttgag
541 cgtgtgggac agtttatgga tgttgtacag gataatgtcc agaaggtcga tagaaagcgt
601 gagaaacagc gtacagacga tttagagatg tagaggtact tttatgccga gaaaactttt
661 tgcgtgtgac agtccttaaa atatacttag agcgtaagcg aaagtagtag cgacagctat
721 taactttcgg ttgcaaagct ctaggatttt taatggacgc agcgcatcac acgcaaaaag
781 gaaattggaa taaatgcgaa atttgagatg ttaattaaag acctttttga ggtctttttt
841 tcttagattt ttggggttat ttaggggaga aaacataggg gggtactacg acctcccccc
901 taggtgtcca ttgtccattg tccaaacaaa taaataaata ttgggttttt aatgttaaaa
961 ggttgttttt tatgttaaag tgaaaaaaac agatgttggg aggtacagtg atggttgtag
1021 atagaaaaga agagaaaaaa gttgctgtta ctttaagact tacaacagaa gaaaatgaga
1081 tattaaatag aatcaaagaa aaatataata ttagcaaatc agatgcaacc ggtattctaa
1141 taaaaaaata tgcaaaggag gaatacggtg cattttaaac aaaaaaagat agacagcact
1201 ggcatgctgc ctatctatga ctaaattttg ttaagtgtat tagcaccgtt attatatcat
1261 gagcgaaaat gtaataaaag aaactgaaaa caagaaaaat tcaagaggac gtaattggac
1321 atttgtttta tatccagaat cagcaaaagc cgagtggtta gagtatttaa aagagttaca
1381 cattcaattt gtagtgtctc cattacatga tagggatact gatacagaag gtaggatgaa
1441 aaaagagcat tatcatattc tagtgatgta tgagggtaat aaatcttatg aacagataaa
1501 aataattaac agaagaattg aatgcgacta ttccgcagat tgcaggaagt gtgaaaggtc
1561 ttgtgagata tatgcttcac atggacgatc ctaataaatt taaatatcaa aaagaagata
1621 tgatagttta tggcggtgta gatgttgatg aattattaaa gaaaacaaca acagatagat
1681 ataaattaat taaagaaatg attgagttta ttgatgaaca aggaatcgta gaatttaaga
1741 gtttaatgga ttatgcaatg aagtttaaat ttgatgattg gttcccgctt ttatgtgata
1801 actcggcgta tgttattcaa gaatatataa aatcaaatcg gtataaatct gaccgataga
1861 ttttgaattt aggtgtcaca agacactctt ttttcgcacc agcgaaaact ggtttaagcc
1921 gactgcgcaa aagacataat cgattcacaa aaaataggca cacgaaaaac aagttaaggg
1981 atgcagttta tgcatccctt aacttactta ttaaataatt tatagctatt gaaaagagat
2041 aagaattgtt caaagctaat attgtttaaa tcgtcaattc ctgcatgttt taaggaattg
2101 ttaaattgat tttttgtaaa tattttcttg tattctttgt taacccattt cataacgaaa
2161 taattatact tttgtttatc tttgtgtgat attcttgatt tttttctact taatctgata
2221 agtgagctat tcactttagg tttaggatga aaatattctc ttggaaccat acttaatata
2281 gaaatatcaa cttctgccat taaaagtaat gccaatgagc gttttgtatt taataatctt
2341 ttagcaaacc cgtattccac gattaaataa atctcattag ctatactatc aaaaacaatt
2401 ttgcgtatta tatccgtact tatgttataa ggtatattac catatatttt ataggattgg
2461 tttttaggaa atttaaactg caatatatcc ttgtttaaaa cttggaaatt atcgtgatca
2521 acaagtttat tttctgtagt tttgcataat ttatggtcta tttcaatggc agttacgaaa
2581 ttacacctct ttactaattc aagggtaaaa tggccttttc ctgagccgat ttcaaagata
2641 ttatcatgtt catttaatct tatatttgtc attattttat ctatattatg ttttgaagta
2701 ataaagtttt gactgtgttt tatatttttc tcgttcatta taaccctctt taatttggtt
2761 atatgaattt tgcttattaa cgattcatta taaccactta ttttttgttt ggttgataat
2821 gaactgtgct gattacaaaa atactaaaaa tgcccatatt ttttcctcct tataaaatta
2881 gtataattat agcacgagct ctgataaata tgaacatgat gagtgatcgt taaatttata
2941 ctgcaatcgg atgcgattat tgaataaaag atatgagaga tttatctaat ttcttttttc
3001 ttgtaaaaaa agaaagttct taaaggtttt atagttttgg tcgtagagca cacggtttaa
3061 cgacttaatt acgaagtaaa taagtctagt gtgttagact ttatgaaatc tatatacgtt
3121 tatatatatt tattatccgg aggtgtagca tgtctcattc aattttgagg gttgccagag
3181 ttaaaggatc aagtaataca aacgggatac aaagacataa tcaaagagag aataaaaact
3241 ataataataa agacataaat catgaggaaa catataaaaa ttatgatttg attaacgcac
3301 aaaatataaa gtataaagat aaaattgatg aaacgattga tgagaattat tcagggaaac
3361 gtaaaattcg gtcagatgca attcgacatg tggacggact ggttacaagt gataaagatt
3421 tctttgatga tttaagcgga gaagaaatag aacgattttt taaagatagc ttggagtttc
3481 tagaaaatga atacggtaag gaaaatatgc tgtatgcgac tgtccatctg gatgaaagag
3541 tcccacatat gcactttggt tttgtccctt taacagagga cgggagattg tctgcaaaag
3601 aacagttagg caacaagaaa gactttactc aattacaaga tagatttaat gagtatgtga
3661 atgagaaagg ttatgaactt gaaagaggca cgtccaaaga ggttacagaa cgagaacata
3721 aagcgatg
//
