| 质粒名称: | pAAV-CAG-IFP2 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000059 |
| 产品用途: | AAV, Expresses IFP2 in cytoplasm of mammalian cells |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CAG |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a, TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | IFP2 |
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳动物表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Quantitative assessment of near-infrared fluorescent proteins
Zhang H., Papadaki S., Sun X., Wang X., Drobizhev M., Yao L., Rehbock M., Köster R.W., Wu L., Namikawa K., Piatkevich K.D.
Nat. Methods 2023 2010 20, 1605–1616 (2023)
|
pAAV-CAG-IFP2
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAV-CAG-IFP2载体质粒基本信息
pAAV-CAG-IFP2质粒图谱载体图谱和pAAV-CAG-IFP2载体序列质粒序列多克隆位点信息
pAAV-CAG-IFP2质粒载体简介
pAAV-CAG-IFP2质粒序列载体序列
LOCUS pAAV-CAG-IFP2 5689 bp DNA circular SYN 21-APR-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5689)
AUTHORS Triple Threat
TITLE Direct Submission
JOURNAL Exported Jun 25, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5689
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 279..556
/label=chicken beta-actin promoter
primer_bind 956..1001
/label=1108-IFP2-F
regulatory 977..986
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 983..1945
/codon_start=1
/product="bacteriophytochrome-based monomeric infrared
fluorescent protein (Yu et al., 2014)"
/label=IFP2.0
/translation="MARDPQPFFPPLYLGGPEITTENCEREPIHIPGSIQPHGALLTAD
GHSGEVLQVSLNAATFLGQEPTVLRGQTLAALLPDQWPALQTALPPGCQDALQYRATLD
WPAAGHLSLTVHRVAELLILEFEPTEAWDSIGPHALRNAMFALESAPNLRALAEVATQT
VRELSGFDRVMLYKFAPDATGEVIAEARREGMQAYLGHRFPASTTPAQARALYTRHLLR
LTADTRAAAVPLDPVLNPQTNAPTPLGGAVLRATSPMHMQYLRNMGVGSSLSVSVVVGG
QLWGLIVCHHQTPYVLPPDLRTTLEYLGRLLSLQVQRKEA"
primer_bind complement(1924..1974)
/label=0503-IFP2-R
misc_feature 1973..2561
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
CDS complement(2444..2455)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 2604..2725
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(3253..3841)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4012..4872)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4873..4977)
/gene="bla"
/label=AmpR promoter
rep_origin complement(5003..5458)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
ORIGIN
1 tctacgtagc catgctctag gaagagtacc attgacgtca ataatgacgt atgttcccat
61 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
121 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
181 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
241 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
301 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
361 attattttgt gcagcgatgg gggcgggggg gggggggggg cgcgcgccag gcggggcggg
421 gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg
481 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg
541 aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc
601 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg
661 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt
721 tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg
781 ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg
841 gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct
901 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag
961 aattggatcc ggtaccgcca ccatggctcg ggaccctcaa cctttcttcc ctcctctgta
1021 tctcggagga ccagagatca ctactgagaa ctgcgagcgg gaacctatac atataccagg
1081 ttctattcaa cctcatggag cactgctcac agccgatgga cattctggcg aggtgctgca
1141 agtttcactc aacgcagcca ctttcctggg ccaggaacca actgttctgc ggggacagac
1201 cctggcagcc ctgctgccag accagtggcc tgctctgcag actgccctgc ctccaggctg
1261 ccaggacgcc ctgcagtatc gcgccaccct ggattggccc gccgccggac acctgagcct
1321 gaccgtgcat agggtggccg agctgctgat tctggaattc gagcctactg aggcatggga
1381 ctctattgga ccccacgctc tgagaaatgc aatgttcgct ctggagagtg ctcctaatct
1441 ccgggcactg gctgaagtcg caacccaaac agtccgggaa ctgtcaggtt tcgaccgggt
1501 gatgctgtac aagtttgcac cagacgcaac aggagaggtt attgccgaag caaggcgcga
1561 gggcatgcag gcttacctcg ggcataggtt tcccgcatcc accacccctg cacaagctag
1621 ggccctctac acaagacacc tgctccggct gaccgcagac accagggctg cagcagtgcc
1681 cctcgacccc gtgctgaatc cccagacaaa tgctcctaca cctctgggcg gagctgtcct
1741 cagagctaca tccccaatgc acatgcagta cctgaggaat atgggagtgg gctcctccct
1801 gagcgtcagc gtcgtggtcg gcggccagct gtggggactg attgtctgcc accatcagac
1861 accctacgtg ctgccacccg atctgcggac caccctggag tatctgggga ggctcctgtc
1921 cctgcaggtg cagaggaaag aagcctaaga attcgatatc aagcttatcg ataatcaacc
1981 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
2041 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
2101 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
2161 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
2221 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
2281 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
2341 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
2401 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
2461 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
2521 ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacccgg
2581 gcggccgctt cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag
2641 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
2701 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
2761 tcagggggag atgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtaaaat
2821 cgataaggat cttcctagag catggctacg tagataagta gcatggcggg ttaatcatta
2881 actacaagga acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca
2941 ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga
3001 gcgagcgagc gcgcagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
3061 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
3121 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
3181 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
3241 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
3301 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
3361 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
3421 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
3481 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
3541 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
3601 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
3661 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
3721 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
3781 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
3841 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
3901 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
3961 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
4021 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
4081 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
4141 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
4201 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
4261 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
4321 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
4381 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
4441 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
4501 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
4561 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
4621 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
4681 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
4741 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
4801 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
4861 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
4921 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
4981 atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta
5041 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
5101 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
5161 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
5221 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
5281 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
5341 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
5401 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc
5461 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
5521 ttacgccagc tgcgcgctcg ctcgctcact gaggccgccc gggcaaagcc cgggcgtcgg
5581 gcgacctttg gtcgcccggc ctcagtgagc gagcgagcgc gcagagaggg agtggccaac
5641 tccatcacta ggggttcctt gtagttaatg attaacccgc catgctact
//
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