LOCUS pCambia1300-35S 9566 bp DNA circular SYN 12-JUN-2025
DEFINITION .
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9566)
AUTHORS Ming
TITLE Direct Submission
JOURNAL Exported Jun 12, 2025 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9566
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 6..351
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
/note="color: #ffffff; direction: RIGHT"
terminator 405..657
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
/note="color: #ffffff; direction: RIGHT"
primer_bind complement(667..683)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
/note="color: #a020f0; direction: LEFT"
misc_feature 886..910
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
/note="color: #ffe4c4"
CDS 2210..2839
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 StaA
/note="color: #993366"
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
CDS 3268..4341
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 RepA
/note="color: #993366"
/translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
LVMRYRNLIEGEASAGS"
rep_origin 4407..4601
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/note="color: #ffff00"
misc_feature 4945..5085
/label=bom
/note="basis of mobility region from pBR322"
/note="color: #a6acb3"
rep_origin complement(5271..5859)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
/note="color: #ffff00"
CDS complement(5946..6740)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
/note="color: #ccffcc"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
misc_feature 7165..7189
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
/note="color: #ffe4c4"
polyA_signal 7267..7441
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
/note="color: #a6acb3"
CDS complement(7481..8506)
/codon_start=1
/product="hygromycin B phosphotransferase"
/label=HygR
/note="confers resistance to hygromycin"
/note="color: #ccffcc"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
K"
promoter complement(8574..9251)
/label=CaMV 35S promoter (enhanced)
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
/note="color: #ffffff; direction: LEFT"
protein_bind 9442..9463
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
/note="color: #31849b"
promoter 9478..9508
/label=lac promoter
/note="promoter for the E. coli lac operon"
/note="This forward directional feature has 3 segments:
1: 9478 .. 9483 / #ffffff / -35
2: 9484 .. 9501 / #ffffff
3: 9502 .. 9508 / #ffffff / -10"
protein_bind 9516..9532
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/note="color: #31849b"
primer_bind 9540..9556
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
/note="color: #a020f0; direction: RIGHT"
ORIGIN
1 aattctgaga cttttcaaca aagggtaata tccggaaacc tcctcggatt ccattgccca
61 gctatctgtc actttattgt gaagatagtg gaaaaggaag gtggctccta caaatgccat
121 cattgcgata aaggaaaggc catcgttgaa gatgcctctg ccgacagtgg tcccaaagat
181 ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag
241 caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct
301 tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagagaac acgggggacg
361 agctcggtac ccggggatcc tctagagtcg acctgcaggc atgcgatcgt tcaaacattt
421 ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat
481 ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga
541 gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa
601 tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcaag
661 cttggcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
721 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
781 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgctagagca gcttgagctt
841 ggatcagatt gtcgtttccc gccttcagtt taaactatca gtgtttgaca ggatatattg
901 gcgggtaaac ctaagagaaa agagcgttta ttagaataac ggatatttaa aagggcgtga
961 aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac cacagggttc ccctcgggat
1021 caaagtactt tgatccaacc cctccgctgc tatagtgcag tcggcttctg acgttcagtg
1081 cagccgtctt ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc
1141 ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc gcataaagta gaatacttgc
1201 gactagaacc ggagacatta cgccatgaac aagagcgccg ccgctggcct gctgggctat
1261 gcccgcgtca gcaccgacga ccaggacttg accaaccaac gggccgaact gcacgcggcc
1321 ggctgcacca agctgttttc cgagaagatc accggcacca ggcgcgaccg cccggagctg
1381 gccaggatgc ttgaccacct acgccctggc gacgttgtga cagtgaccag gctagaccgc
1441 ctggcccgca gcacccgcga cctactggac attgccgagc gcatccagga ggccggcgcg
1501 ggcctgcgta gcctggcaga gccgtgggcc gacaccacca cgccggccgg ccgcatggtg
1561 ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc taatcatcga ccgcacccgg
1621 agcgggcgcg aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc taccctcacc
1681 ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag
1741 gcggctgcac tgcttggcgt gcatcgctcg accctgtacc gcgcacttga gcgcagcgag
1801 gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc attgaccgag
1861 gccgacgccc tggcggccgc cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc
1921 aggacggcca ggacgaaccg tttttcatta ccgaagagat cgaggcggag atgatcgcgg
1981 ccgggtacgt gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat gaaatcctgg
2041 ccggtttgtc tgatgccaag ctggcggcct ggccggccag cttggccgct gaagaaaccg
2101 agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg
2161 ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa ggggaacgca tgaaggttat
2221 cgctgtactt aaccagaaag gcgggtcagg caagacgacc atcgcaaccc atctagcccg
2281 cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat tccgatcccc agggcagtgc
2341 ccgcgattgg gcggccgtgc gggaagatca accgctaacc gttgtcggca tcgaccgccc
2401 gacgattgac cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc
2461 gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc
2521 ggtgcagcca agcccttacg acatatgggc caccgccgac ctggtggagc tggttaagca
2581 gcgcattgag gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa
2641 aggcacgcgc atcggcggtg aggttgccga ggcgctggcc gggtacgagc tgcccattct
2701 tgagtcccgt atcacgcagc gcgtgagcta cccaggcact gccgccgccg gcacaaccgt
2761 tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat
2821 taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg
2881 ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg caacgttggc cagcctggca
2941 gacacgccag ccatgaagcg ggtcaacttt cagttgccgg cggaggatca caccaagctg
3001 aagatgtacg cggtacgcca aggcaagacc attaccgagc tgctatctga atacatcgcg
3061 cagctaccag agtaaatgag caaatgaata aatgagtaga tgaattttag cggctaaagg
3121 aggcggcatg gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg
3181 aggaacgggc ggttggccag gcgtaagcgg ctgggttgtc tgccggccct gcaatggcac
3241 tggaaccccc aagcccgagg aatcggcgtg agcggtcgca aaccatccgg cccggtacaa
3301 atcggcgcgg cgctgggtga tgacctggtg gagaagttga aggccgcgca ggccgcccag
3361 cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga
3421 atccgcaaag aatcccggca accgccggca gccggtgcgc cgtcgattag gaagccgccc
3481 aagggcgacg agcaaccaga ttttttcgtt ccgatgctct atgacgtggg cacccgcgat
3541 agtcgcagca tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg acgagctggc
3601 gaggtgatcc gctacgagct tccagacggg cacgtagagg tttccgcagg gccggccggc
3661 atggccagtg tgtgggatta cgacctggta ctgatggcgg tttcccatct aaccgaatcc
3721 atgaaccgat accgggaagg gaagggagac aagcccggcc gcgtgttccg tccacacgtt
3781 gcggacgtac tcaagttctg ccggcgagcc gatggcggaa agcagaaaga cgacctggta
3841 gaaacctgca ttcggttaaa caccacgcac gttgccatgc agcgtacgaa gaaggccaag
3901 aacggccgcc tggtgacggt atccgagggt gaagccttga ttagccgcta caagatcgta
3961 aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc tagctgattg gatgtaccgc
4021 gagatcacag aaggcaagaa cccggacgtg ctgacggttc accccgatta ctttttgatc
4081 gatcccggca tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa
4141 gccagatggt tgttcaagac gatctacgaa cgcagtggca gcgccggaga gttcaagaag
4201 ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta cgatttgaag
4261 gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct accgcaacct gatcgagggc
4321 gaagcatccg ccggttccta atgtacggag cagatgctag ggcaaattgc cctagcaggg
4381 gaaaaaggtc gaaaaggtct ctttcctgtg gatagcacgt acattgggaa cccaaagccg
4441 tacattggga accggaaccc gtacattggg aacccaaagc cgtacattgg gaaccggtca
4501 cacatgtaag tgactgatat aaaagagaaa aaaggcgatt tttccgccta aaactcttta
4561 aaacttatta aaactcttaa aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca
4621 gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg ccccgccgct
4681 tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat
4741 ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc
4801 accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
4861 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
4921 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt
4981 gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
5041 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc
5101 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
5161 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
5221 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
5281 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
5341 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
5401 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
5461 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
5521 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
5581 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
5641 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
5701 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
5761 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
5821 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
5881 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgcattct
5941 aggtactaaa acaattcatc cagtaaaata taatatttta ttttctccca atcaggcttg
6001 atccccagta agtcaaaaaa tagctcgaca tactgttctt ccccgatatc ctccctgatc
6061 gaccggacgc agaaggcaat gtcataccac ttgtccgccc tgccgcttct cccaagatca
6121 ataaagccac ttactttgcc atctttcaca aagatgttgc tgtctcccag gtcgccgtgg
6181 gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga
6241 tctttaaatg gagtgtcttc ttcccagttt tcgcaatcca catcggccag atcgttattc
6301 agtaagtaat ccaattcggc taagcggctg tctaagctat tcgtataggg acaatccgat
6361 atgtcgatgg agtgaaagag cctgatgcac tccgcataca gctcgataat cttttcaggg
6421 ctttgttcat cttcatactc ttccgagcaa aggacgccat cggcctcact catgagcaga
6481 ttgctccagc catcatgccg ttcaaagtgc aggacctttg gaacaggcag ctttccttcc
6541 agccatagca tcatgtcctt ttcccgttcc acatcatagg tggtcccttt ataccggctg
6601 tccgtcattt ttaaatatag gttttcattt tctcccacca gcttatatac cttagcagga
6661 gacattcctt ccgtatcttt tacgcagcgg tatttttcga tcagtttttt caattccggt
6721 gatattctca ttttagccat ttattatttc cttcctcttt tctacagtat ttaaagatac
6781 cccaagaagc taattataac aagacgaact ccaattcact gttccttgca ttctaaaacc
6841 ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat aacatagtat
6901 cgacggagcc gattttgaaa ccgcggtgat cacaggcagc aacgctctgt catcgttaca
6961 atcaacatgc taccctccgc gagatcatcc gtgtttcaaa cccggcagct tagttgccgt
7021 tcttccgaat agcatcggta acatgagcaa agtctgccgc cttacaacgg ctctcccgct
7081 gacgccgtcc cggactgatg ggctgcctgt atcgagtggt gattttgtgc cgagctgccg
7141 gtcggggagc tgttggctgg ctggtggcag gatatattgt ggtgtaaaca aattgacgct
7201 tagacaactt aataacacat tgcggacgtt tttaatgtac tgaattaacg ccgaattaat
7261 tcgggggatc tggattttag tactggattt tggttttagg aattagaaat tttattgata
7321 gaagtatttt acaaatacaa atacatacta agggtttctt atatgctcaa cacatgagcg
7381 aaaccctata ggaaccctaa ttcccttatc tgggaactac tcacacatta ttatggagaa
7441 actcgagctt gtcgatcgac agatccggtc ggcatctact ctatttcttt gccctcggac
7501 gagtgctggg gcgtcggttt ccactatcgg cgagtacttc tacacagcca tcggtccaga
7561 cggccgcgct tctgcgggcg atttgtgtac gcccgacagt cccggctccg gatcggacga
7621 ttgcgtcgca tcgaccctgc gcccaagctg catcatcgaa attgccgtca accaagctct
7681 gatagagttg gtcaagacca atgcggagca tatacgcccg gagtcgtggc gatcctgcaa
7741 gctccggatg cctccgctcg aagtagcgcg tctgctgctc catacaagcc aaccacggcc
7801 tccagaagaa gatgttggcg acctcgtatt gggaatcccc gaacatcgcc tcgctccagt
7861 caatgaccgc tgttatgcgg ccattgtccg tcaggacatt gttggagccg aaatccgcgt
7921 gcacgaggtg ccggacttcg gggcagtcct cggcccaaag catcagctca tcgagagcct
7981 gcgcgacgga cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac acatggggat
8041 cagcaatcgc gcatatgaaa tcacgccatg tagtgtattg accgattcct tgcggtccga
8101 atgggccgaa cccgctcgtc tggctaagat cggccgcagc gatcgcatcc atagcctccg
8161 cgaccggttg tagaacagcg ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct
8221 gtgcacggcg ggagatgcaa taggtcaggc tctcgctaaa ctccccaatg tcaagcactt
8281 ccggaatcgg gagcgcggcc gatgcaaagt gccgataaac ataacgatct ttgtagaaac
8341 catcggcgca gctatttacc cgcaggacat atccacgccc tcctacatcg aagctgaaag
8401 cacgagattc ttcgccctcc gagagctgca tcaggtcgga gacgctgtcg aacttttcga
8461 tcagaaactt ctcgacagac gtcgcggtga gttcaggctt tttcatatct cattgccccc
8521 cgggatctgc gaaagctcga gagagataga tttgtagaga gagactggtg atttcagcgt
8581 gtcctctcca aatgaaatga acttccttat atagaggaag gtcttgcgaa ggatagtggg
8641 attgtgcgtc atcccttacg tcagtggaga tatcacatca atccacttgc tttgaagacg
8701 tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac
8761 cactgtcggc agaggcatct tgaacgatag cctttccttt atcgcaatga tggcatttgt
8821 aggtgccacc ttccttttct actgtccttt tgatgaagtg acagatagct gggcaatgga
8881 atccgaggag gtttcccgat attacccttt gttgaaaagt ctcaatagcc ctttggtctt
8941 ctgagactgt atctttgata ttcttggagt agacgagagt gtcgtgctcc accatgttat
9001 cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc
9061 tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttga acgatagcct
9121 ttcctttatc gcaatgatgg catttgtagg tgccaccttc cttttctact gtccttttga
9181 tgaagtgaca gatagctggg caatggaatc cgaggaggtt tcccgatatt accctttgtt
9241 gaaaagtctc aatagccctt tggtcttctg agactgtatc tttgatattc ttggagtaga
9301 cgagagtgtc gtgctccacc atgttggcaa gctgctctag ccaatacgca aaccgcctct
9361 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
9421 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
9481 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
9541 aggaaacagc tatgaccatg attacg
//