| 质粒名称: | pAAVLINK-EP400-B |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001748 |
| 产品用途: | Body' AAVLINK plasmid for EP400 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | Promoterless |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | EP400 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EP400-B
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EP400-B载体质粒基本信息
pAAVLINK-EP400-B质粒图谱载体图谱和pAAVLINK-EP400-B载体序列质粒序列多克隆位点信息
pAAVLINK-EP400-B质粒载体简介
pAAVLINK-EP400-B质粒序列载体序列
LOCUS pAAVLINK-EP400-B 6745 bp DNA circular SYN 22-JAN-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6745)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 5, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6745
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..141
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..141
/label=AAV2 ITR
protein_bind 156..189
/label=loxJTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 190..281
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 282..3901
/codon_start=1
/label=EP400
/translation="VIGAPILLL*EVVTYSSGSLN*NVGVPDSKSSHILAATENSKQRD
RSGPNPTASTSASRPTLSSSGASPPSHECAGSAWSLMRCSA*RA*PRGTGKRFSPCRAN
NVCF*STRRCTIPSWSSGPWCTSWSQGSPGPT*APL*GPPVKRARITTIKWS*GYTG*H
SHLF*GELREMWKSN*QRNMSMF*SVAFLTDKKPYTRTLSCNLALRRP*RAGTLSTS*A
SLCGCSASATTLGSSSPGTQALPTWRGHWSIRPHL*S*RHWREISGRKQIFLCLISSA*
KIKSLVTRQSCCLRKRYRGNSWRKSPLQQPQQPDQQQQS*RPAGCFSLCSMARSPRVAP
WLSPALTRPGRQPPPRPLLLHRARFEDGRPSPRSLPIRRQKQQQPRFRPLRLPPVLHDT
SPPRPPAQPLARPILRNCGPRPQHRPPPQASPRPSPRPPRTRPGRARCLRGWCSPRRPR
PACPVER**K*LSWHPSQDHRAAWLSQRRR*HCSSRAASSPCHTASSGSSQRASRCSCK
AASSRSCPPPGSPTFEPLALW*CRPCLRRALCTAPWEASPRPAVPALHP*PHKLAFRAA
WR*MPWL*ENPERPPNQLLPLEGRPRRKRPDS*KSAWIRFI*STSGAVLKLQSMAETC*
GFVPCLAMEGYSGVGPWMAVVGRRPGQRTVTLHPQKVQVS*C*RFVGVESLCRMLLTGW
PL*FLRWWQHPRPYGCRGRHPCTATE*GS*GRA*ESTLRRTSSSCGRPRLHACCSSLS*
GWCSSTQGSWKL*LSCFRN*NLKDVGC*FYHR*FLCWTF*RCS*TSITSPM*ESMKMPA
VSNGRN**GVSTETGGFFVPFSPLTAVPQV*TL*RRTPSCFMTMT*IQ*WMPKLRSGAI
GSGDAKTSTYTGL*VAIPLKRNC*KMELKI*SEKWLLREMTTPWLS*LSEPSRSCLKFI
LPWMMLASRSKLRSLWCFLRNLLSRKPLHPKLQDLS*RPSRVLSIWRRMPRSPHRRGCW
DHTLMLCHQTLRTCRVMKNHPN*RS*LTSWSSLHQLKNML*ITWNYSILLLSKKRRETV
RTQ**LQ*GHGSSGT*RPCRRGRPGCGWSRRRRSS*PTRERMPTAWSMSTKMSMGRQKS
CRSGPHPPRRRTTATSTSTRSCVSCMKPLPSQRLSCPLCT*GRSGSDTKQTPQLQAGRR
SSVTGRRSSLLGPCLTAQHQDF*KFAERARSRRRIFC*SS"
misc_feature 906..911
/label=SDS
misc_feature 2085..2090
/label=SDS
misc_feature 2253..2258
/label=SDS
misc_feature 3210..3215
/label=SDS
misc_feature 3234..3239
/label=SDS
misc_feature 3513..3518
/label=SDS
misc_feature 3516..3521
/label=SDS
misc_feature 3806..3811
/label=SDS
intron 3902..3942
/label=Splicing Donor
misc_feature 3946..3979
/label=lox2272-JT1571
repeat_region 4008..4148
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4008..4137
/label=AAV2 ITR
rep_origin 4223..4678
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4960..5064
/gene="bla"
/label=AmpR promoter
CDS 5065..5925
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6096..6684
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
121 actccatcac taggggttcc tgcggccgca cgcgtataac ttcgtatagc atacattata
181 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
241 tattggtctt actgacatcc actttgcctt tctctccaca ggtaattggg gcccccatct
301 tgttgttgtg agaagttgta acatactcaa gtgggagctt gaattgaaac gttggtgtcc
361 cggactcaaa atcctctcat atattggcag ccacagagaa ctcaaagcaa agagacagga
421 gtgggccgaa cccaacagct tccacgtctg catcacgtcc tacactcagt tcttccgggg
481 cctcaccgcc ttcacacgag tgcgctggaa gtgcctggtc attgatgaga tgcagcgcgt
541 gaagggcatg accgagaggc actgggaagc ggttttcacc ctgcagagcc aacaacgtct
601 gcttctgatc gactcgccgc tgcacaatac cttcctggag ctctggacca tggtgcactt
661 cctggtccca gggatctcca ggccctacct gagctcccct ctgagggccc ccagtgaaga
721 gagccaggat tactaccata aagtggtcat aaggttacac agggtgacac agccatttat
781 tttgaggaga actaagagag atgtggaaaa gcaactaaca aagaaatatg agcatgtttt
841 gaagtgtcgc ctttctaacc gacaaaaagc cttatacgag gacgttatcc tgcaacctgg
901 cactcaggag gccttgaaga gcgggcactt tgtcaacgtc ctgagcatcc ttgtgcggct
961 gcagcgcatc tgcaaccacc ctgggctcgt cgagccccgg cacccaggct cttcctacgt
1021 ggcggggcca ctggagtatc cgtccgcatc tctaatcctg aaggcactgg agagagattt
1081 ctggaaggaa gcagatcttt ctatgtttga tctcatcggc ttagaaaata aaatcactcg
1141 tcacgaggca gagttgctgt ctaagaaaaa gataccgcgg aaactcatgg aggaaatctc
1201 cacttcagca gccccagcag cccgaccagc agcagcaaag ctgaaggcca gcaggttgtt
1261 tcagcctgtg cagtatggcc agaagcccga gggtcgcacc gtggctttcc ccagcactca
1321 cccgccccgg acggcagccc ccaccacggc ctctgctgct ccacagggcc cgcttcgagg
1381 acggccgccc atcgccacgt tctctgccaa tccggaggca aaagcagcag cagccccgtt
1441 tcagacctct caggcttccg ccagtgctcc acgacaccag cccgcctcgg cctccagcac
1501 agccgctagc ccggcccatc ctgcgaaact gcgggcccag accacagcac aggcctccac
1561 cccaggccag cccccgcccc agccccaggc cccctcgcac gcggccgggc agagcgcgct
1621 gcctcagagg ctggtgctcc cctcgcaggc ccaggcccgc ttgcccagtg gagaggtagt
1681 gaaaatagct cagctggcat ccatcacagg accacagagc cgcgtggctc agccagagac
1741 gccggtgaca ctgcagttcc agggcagcaa gttcaccctg tcacacagcc agctccggca
1801 gctcacagcg ggccagccgc tgcagctgca aggcagcgtc ctccagatcg tgtccgcccc
1861 cgggcagccc taccttcgag cccctggccc tgtggtgatg cagaccgtgt ctcaggcggg
1921 cgctgtgcac ggcgccctgg gaagcaagcc cccggccggc ggtcccagcc ctgcaccctt
1981 gaccccacaa gttggcgttc cgggccgcgt ggcggtgaat gccttggctg taggagaacc
2041 cggaacggcc tccaaaccag cttctcccat tggagggccg acccaggagg aaaagaccag
2101 actcttgaaa gagcgcctgg atcagattta tttagtcaac gagcggcgct gttctcaagc
2161 tccagtctat ggcagagact tgctaaggat ttgtgccctg cctagccatg gaagggtaca
2221 gtggcgtggg tccctggatg gccgtcgtgg gaaggaggcc gggccagcgc acagttacac
2281 ttcatcctca gaaagtccaa gtgagctgat gttgacgctt tgtcggtgtg gagagtctct
2341 gcaggatgtt attgacaggg tggcctttgt gattcctccg gtggtggcag cacccccgtc
2401 cctacgggtg ccgcggccgc cacccctgta cagccacaga atgaggatct tgaggcaggg
2461 cctgagagag cacgctgcgc cgtacttcca gcagctgcgg cagaccacgg ctccacgcct
2521 gctgcagttc cctgagctga ggctggtgca gttcgactca gggaagttgg aagctttagc
2581 tatcttgctt cagaaattga aatctgaagg acgtcgggtg ctgattttat cacagatgat
2641 tcttatgttg gacattttag agatgttctt gaacttccat tacctcacct atgtaagaat
2701 cgatgaaaat gccagcagtg agcaacggca ggaactgatg aggagtttca acagagacag
2761 gcggattttt tgtgccattc tctccactca cagccgtacc acaggtataa accttgtaga
2821 ggcggacacc gtcgtgtttt atgacaatga cctgaatcca gtgatggatg ccaaagctca
2881 ggagtggtgc gataggatcg ggagatgcaa agacatccac atatacaggc ttgtgagtgg
2941 caattccatt gaagagaaat tgttgaaaaa tggaactaaa gatctgatcc gagaagtggc
3001 tgctcaggga aatgactact ccatggcttt cttaactcag cgaaccatcc aggagctgtt
3061 tgaagtttat tctcccatgg atgatgctgg cttcccggtc aaagctgagg agtttgtggt
3121 gctttctcag gaaccttctg tcacggaaac cattgcaccc aaaattgcaa gacctttcat
3181 agaggccctc aagagtattg agtatctgga ggaggatgcc cagaagtccg cacaggaggg
3241 ggtgctggga ccacacactg atgctctgtc atcagactct gagaacatgc cgtgtgatga
3301 agaaccatcc caattagagg agctagctga cttcatggag cagcttacac caattgaaaa
3361 atatgcttta aattacctgg aattattcca tacttctatt gagcaagaaa aggagagaaa
3421 cagtgaggac gcagtgatga ctgcagtgag ggcatgggag ttctggaacc tgaagaccct
3481 gcaggagagg gaggcccggc tgcggctgga gcaggaggag gcggagctcc tgacctacac
3541 gcgagaggat gcctacagca tggagtatgt ctacgaagat gtcgatgggc agacagaagt
3601 catgccgctc tggaccccac ccaccccgcc gcaggacgac agcgacatct acctcgactc
3661 ggtcatgtgt ctcatgtatg aagccactcc catcccagag gctaagctgc cccctgtgta
3721 cgtgaggaag gagcggaagc gacacaaaac agacccctca gctgcaggca ggaagaagaa
3781 gcagcgtcac ggggaggcgg tcgtccctcc tcggtccctg tttgaccgcg caacaccagg
3841 acttctgaaa attcgcagag agggcaagga gcagaagaag aatattctgc tgaagcagca
3901 ggtaagtatc aaggttacaa gacaggttta aggagaccaa taagctatta ttcgtatagg
3961 atactttata cgaagttatg aattccacgt gcggaccgag cggccgcagg aacccctagt
4021 gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa
4081 ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg
4141 cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
4201 catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
4261 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
4321 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
4381 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg
4441 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
4501 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
4561 tcgggctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
4621 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta
4681 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
4741 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
4801 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
4861 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
4921 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
4981 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
5041 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
5101 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
5161 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
5221 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
5281 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
5341 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
5401 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
5461 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
5521 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
5581 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
5641 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
5701 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
5761 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
5821 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
5881 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
5941 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
6001 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
6061 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
6121 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
6181 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
6241 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
6301 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
6361 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
6421 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
6481 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
6541 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
6601 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
6661 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
6721 ttttgctggc cttttgctca catgt
//
