pBAD-bARGSer-AxeTxe

LOCUS       pBAD-bARGSer-AxeTxe               23202 bp ds-DNA     circular SYN 08-NOV-2022
DEFINITION  Second-generation bacterial acoustic reporter gene.
ACCESSION   .
VERSION     .
KEYWORDS    pBAD-bARGSer-AxeTxe
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 23202)
  TITLE     Genomically mined acoustic reporter genes enable real-time in vivo 
            monitoring of tumors and tumor-homing probiotics
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 23202)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 8, 2022 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..23202
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..546
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     447..466
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     663..680
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        1072..1174
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1175..1834
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWCEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1241..1260)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      complement(2390..2845)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2527..2548)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2739..2758
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        complement(3303..3394)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      3413..3440
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      3532..3618
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3771..3788
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     CDS             4895..4906
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(11533..11544)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        complement(20531..20815)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(20589..20608)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             20842..21720
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPQGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     primer_bind     complement(21900..21919)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ttgagatcgt tttggtctgc gcgtaatctc ttgctctgaa aacgaaaaaa ccgccttgca
       61 gggcggtttt tcgaaggttc tctgagctac caactctttg aaccgaggta actggcttgg
      121 aggagcgcag tcaccaaaac ttgtcctttc agtttagcct taaccggcgc atgacttcaa
      181 gactaactcc tctaaatcaa ttaccagtgg ctgctgccag tggtgctttt gcatgtcttt
      241 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcggac tgaacggggg
      301 gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc ggaactgagt gtcaggcgtg
      361 gaatgagaca aacgcggcca taacagcgga atgacaccgg taaaccgaaa ggcaggaaca
      421 ggagagcgca cgagggagcc gccaggggga aacgcctggt atctttatag tcctgtcggg
      481 tttcgccacc actgatttga gcgtcagatt tcgtgatgct tgtcaggggg gcggagccta
      541 tggaaaaacg gctttgccgc ggccctctca cttccctgtt aagtatcttc ctggcatctt
      601 ccaggaaatc tccgccccgt tcgtaagcca tttccgctcg ccgcagtcga acgaccgagc
      661 gtagcgagtc agtgagcgag gaagcggaat atatcctgta tcacatattc tgctgacgca
      721 ccggtgcagc cttttttctc ctgccacatg aagcacttca ctgacaccct catcagtgcc
      781 aacatagtaa gccagtatac actccgctag cgctgatgtc cggcggtgct tttgccgtta
      841 cgcaccaccc cgtcagtagc tgaacaggag ggacagctga tagaaacaga agccactgga
      901 gcacctcaaa aacaccatca tacactaaat cagtaagttg gcagcatcac ccgacgcact
      961 ttgcgccgaa taaatacctg tgacggaaga tcacttcgca gaataaataa atcctggtgt
     1021 ccctgttgat accgggaagc cctgggccaa cttttggcga aaatgagacg ttgatcggca
     1081 cgtaagaggt tccaactttc accataatga aataagatca ctaccgggcg tattttttga
     1141 gttatcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac
     1201 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc
     1261 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa
     1321 gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc
     1381 tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca
     1441 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggtgtgaata
     1501 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga
     1561 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc
     1621 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc
     1681 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat
     1741 tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca
     1801 acagtactgc gatgagtggc agggcggggc gtaatttttt taaggcagtt attggtgccc
     1861 ttaaacgcct ggttgctacg cctgaataag tgataataag cggatgaatg gcagaaattc
     1921 gaaagcaaat tcgacccggt cgtcggttca gggcagggtc gttaaatagc cgcttatgtc
     1981 tattgctggt ttaccggttt attgactacc ggaagcagtg tgaccgtgtg cttctcaaat
     2041 gcctgactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     2101 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     2161 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     2221 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     2281 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     2341 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattgtaaac
     2401 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     2461 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agcccgagat agggttgagt
     2521 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     2581 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccaa atcaagtttt
     2641 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     2701 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     2761 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     2821 cttaatgcgc cgctacaggg cgcgtaaatc aatctaaagt atatatgagt aaacttggtc
     2881 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     2941 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     3001 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     3061 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     3121 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     3181 gctgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     3241 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     3301 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     3361 tacatatttg aatgtattta gaaaaataaa caaaagagtt tgtagaaacg caaaaaggcc
     3421 atccgtcagg atggccttct gcttaatttg atgcctggca gtttatggcg ggcgtcctgc
     3481 ccgccaccct ccgggccgtt gcttcgcaac gttcaaatcc gctcccggcg gatttgtcct
     3541 actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt ctttcgactg
     3601 agcctttcgt tttatttgat gcctggcagt tccctactct cgcatgggga gaccccacac
     3661 taccatcggc gctacggcgt ttcacttctg agttcggcat ggggtcaggt gggaccaccg
     3721 cgctactgcc gccaggcaaa ttctgtttta tcagaccgct tctgcgttct gatttaatct
     3781 gtatcaggct gaaaatcttc tctcaatgtg acggaagtcg cctatcaagt agggtttaat
     3841 agtacaagct attttattaa tcagtttcga aaagaaatga agatgactcc gctcatgtat
     3901 cgaaaaaaga agctgcacat ctcatcacat cacgctaacg ataaaacacc atttgcacct
     3961 taaaacgagc actatcgtca taatgcccgt tttaaggtgc agcgtgtcag taattccccc
     4021 cgaagaagca gagcgttgat taagctagcg acgcaactac tcgctcggct gtgatagtgt
     4081 ctgtaatgtt ttcagcagct ctttattggt aaaaggcttc gccagaaaac cgcagataat
     4141 gccttcaacg atagcatggc gaataaccgg atcatcgtaa tagtgataac cggaaaccag
     4201 cactacacgc ggtcttcgct ctagcaaggt actggctcga cgcgccactt ccagaccgtc
     4261 catatccggt aacttggcat ctaaaaacac aaaggaaaaa ttgccggtca tcagcttaac
     4321 gcaagcatct tccccactgt aagcggtaat gacactcatg ccttgtgatt gaataatatg
     4381 ggaaatggcc caacacatgt caggttcatc atcaactatc agaatgggtg cgttcatcat
     4441 tttagagtat aggtaaggta acaataatag tggaaccttc acccagacaa ctctctgcct
     4501 gtatggaacc aaggtgctgt tggataatgc tgtaacaaat ggacaaaccc aaaccagtac
     4561 cttggcctac gggtgaagac gtgtggaacg gatcgaaaat tttatccaac tcgccggcgg
     4621 gaatgcctac gccagtatca gaaatacgca caataacccg gtcttcaaag gtctctaccg
     4681 caatacttaa cacaccgcct tccggcatcg cattaatggc attaagaaaa atattcatga
     4741 acacctgttg cagtaagccc gacacacctg acaccaatat atttcctttt tgcaacggca
     4801 acaggatctg aattttctgc actttggcct ggttggcgat caggtcgata gtttctttca
     4861 gcaccgtgat cagattgacc tctaccattt cggtatcgag ggaagaacgg gcaaaacgga
     4921 gtaaattctc aatgatggcc gaggcttttt gaatgttagt ctgtattttt cttgaacact
     4981 ctttatgaaa ttcagccgta atctcgtctt ccagcaaaaa ttgcgccgct gaagaacaaa
     5041 cagcaagagg attacgaatt tcatgagcga taccgcccgc catgacgccc aacgcggtca
     5101 acttctgtga ttgacgtaat tgcagttcca atttccgacg ttcagccaaa tcacgaccga
     5161 caactacggt gcccaccgtt tgcccatagt catccttcat tggtgaaaat acccaggaaa
     5221 tcaatatgct gccaccattg ttaggcgtta ataaatgaaa ttcagtcgta tgtgattcat
     5281 tgctgttctt catacgcatg aaaatcagac gcaccgcctc cttttggttg gtatcgcaga
     5341 actcaaaaaa gtatcgccca tagacttcat gcgtgggtaa acctgacagc ttttccgccg
     5401 ctgaattcca cgtcaatatg catccttcaa tatcggtcga aagaacaatg tcgctggcgt
     5461 tttctacaac tgaagcgagg tgccgttcta ctcgatggac ttcttcagac aggcgaacct
     5521 gctcggtgac atcctccatg aacagcataa tctggttaat ggggatgggt agaatagaat
     5581 agtaataaat ccgaatcggt acgcctggcg cacggtaggt cattttctga ccattaacgg
     5641 gtaatccgct acggaataca tcccggatat gtgcttcaag acccgtattt tccaaaatga
     5701 cgctcggaaa gacgtccgat aacgggcgac caattgtatc cgggttgccg cgatgaccct
     5761 tgattaaaaa attctgattg actgaaacca catgcagttt ctcatccacc attaacacgg
     5821 aagaaggaat tgcatccaat agcatgttgt aaagaagttc aaagtggttt tctgactgct
     5881 gttcaaaagg attatctgac atggtagcct gtcgttaaca aattagctgg cgaagttata
     5941 gggaggccaa ggcccactga taacccctat atccgtcatg ttgttggcca gagtattaaa
     6001 ttggtcgcga aacgcctgta acccggttcg tggcaccaga tagctcaaca ggtaagtccg
     6061 ctgaccatta aacatactga tttccgcgca gtgtcggcga tacaaccccg taaacgcatt
     6121 attcagtagt gcagcctgcc gttcagcgtg ttcaggtacg gcataagcga gttttcgact
     6181 ccgcaaatag tccagaccac tcgcttgcgg cgtagtaacg gcattatcct cagccgacgc
     6241 cagggataac cgaataccca tttcgtcaca attttccagc tcaaccaatt gcgtttgata
     6301 atgtgcctca tggttgagta aatgctgtgt taccgcatct tcatctgcca acacacaacc
     6361 ataacgaatc ggaataatcg tggtctgttg atgaacatgc attatctgtt ggccaaaagc
     6421 caacgctgcc gcgggctcgc gactgacgtc tgcttcacaa ggcttgacca gcgccgccaa
     6481 cgttgccgct ttaacaagct gcatcggctc ttcaccggcg tgcggcgaac cgtctggttc
     6541 aagcgccagt tgagtatcct cggcaacaat gccgtacagt agtaagctca tgattcgtca
     6601 ggttggcaaa aactgtaggc tgcccacggg cctgtcacgc gaaaactaaa accgtaagct
     6661 tcatagcgtt caacaatgtc agcaacctgc tgttgaaacg cggcaacgtc ctccaccggg
     6721 agtaaatacg cccaatgcaa aattttatcg tccaacaacc gacgtgaacg gaaatcacgt
     6781 accaaaggat gtaattcgtt ctgcatggca gtcaaagcat gtgccaacca gtcactcaac
     6841 tcagtggtta acctgcggcg taatttctgc tcttccaaat ggcggcggcc tatggcttcg
     6901 ggtaggcaaa agcgaccgga gtccagacct tcggtgaata aaacgtcaac ggcctgttta
     6961 cgatccagtg tcgcttccag tgcccattcc tgacagccgg ttatgcgacg tagcgacaca
     7021 aatacgtcag tagcgcggct tttcatttcc tgttccaacg cgttctgact ggaaaacagc
     7081 gtgccgaagg gcaaaggata gaccggacct tgggccatca ggctgtcaat aatctgcgca
     7141 tgccgacaaa tacgcggcgt tagccaagtg acattctgta cattgtcttc acccttttca
     7201 cccgtgaaat cggagagtgc cactgggcta atcaccgcat tcaatccggc acattgatgt
     7261 gtattaatcg gatgctgatt atcaacgccc aaaattggcg gtgcggctaa atcgggcaac
     7321 gtcaggccat acagataaat ggcctgctca gtctgtgctt ctgtgttcat cgtcatgcat
     7381 catcgccaaa catttcggca atcacttcag caaaatcatg ctgttcaatg cgtaccgtca
     7441 gcgccacagg ggtttctcct accgttgacg ggctcagaac agcctcttca gactgctgga
     7501 ttgcacgacg caatgccgct aatgcggcac gattacacac cgcggccaat tctgcaccgc
     7561 tggcaccatc acaacgttct gccaaatcgt cagcatgaat attgtcaccc agcggtttat
     7621 tacgtaaatg caccgccaga atctgccgac gggcgtcctc gtcgggcagg cccaattcga
     7681 ttatttcatc aaaacgtcct ggtcgcagca tcgcgggatc gatcaagtcc gcacgattgg
     7741 tcgcccccat aacgaaaaca cctttgagtt cttccagccc atccatttca gataagaatt
     7801 ggctcaacac ccgttctgcg acatgtgagt cgatagcgcc ctcgttacgg gcaggaatca
     7861 cagaatcgac ttcatcaagg aaaataatgc agggcgcagc ctgacgagct ttgagaaaca
     7921 gctctcggac acctttttcc gaatcaccga cataacgcga catcagcgcc ggacctttaa
     7981 ctgaaatgac attaacgcca ctttcattag caacggcttt ggcgatcagc gttttcccaa
     8041 cgccgggagg cccagctaac agcagacctt taggcggctt aacacccgct tgttcataga
     8101 gctcagggta cttgataggc cactcgacgg attcgatcaa tcgacggcgt acgtcatcca
     8161 aaccgccgac atcttcccaa cgcacatcgg gaatatcgac gaacagctca cgaatcgctg
     8221 acggtgatac ttcacacaag gcggcacgaa aatcatccat catcaccgtt aattcggcca
     8281 gacgatcata aggtagctcc gccgaactga aatcgatctc aggcagtaaa cggcgtaacg
     8341 cactcatcgc ggcttcacgg cataacgctt ccagatcggc accgacaaaa ccgtgagtga
     8401 tatcggcaag cacgttcaga tcgacatcag cattcagcgg cattcccgtg ctatgaattt
     8461 cgataatctc gcggcgacct tcacggtctg gaataggaat gctgatttca cggtcaaagc
     8521 gtcccggacg gcgcaacgcg ggatcaatcg cattaggtag attggtcgcg gcaatgacaa
     8581 tgactttgcc tcgattcttg agaccatcca ttaatgcgag cagttgcgca acgatacgtt
     8641 tttctacatc accgacgacc ttgtcgcggt gtggtgcaat agagtcaatt tcatccagaa
     8701 aaatgatact tggccctttg cgtccggctt cctcaaaaat tttgcgtaag tgggcttcac
     8761 tttcaccata aaacttatgc acaatttccg ggccgctgat ggtaaaaaat tgcgcatcgg
     8821 tttcttgcgc aataatgcgg gcgatgaggg ttttaccaca tcccggggga ccagagagca
     8881 gtacaccttt gggggcatct atacctaaac gttcaaagac ctcaggatag cgtaatggta
     8941 attcgatcat ttctcgaata cgccgcacct ggtttttaag gccgccaaca tcctcatacg
     9001 acaaacgttg tgtgctatga ctcgaattcc cgacagcatt tgatttgcca atcaccagtg
     9061 tcgtggtcgg atcaatcagt accgcaccat caggtatggt gctttcgact ttaaaatcag
     9121 cagaacgact gccaaacagg tgcgcgcgca gcagatcgcc tttctgtacg ggcaacccat
     9181 cgagcaggct accgatataa tacaggtcac gttggttggg cgtaatcgtg gtgggaatga
     9241 ggacgatctg tgtggcgtgt ttgcaggaag cgggttcaat ctgcactttt tcatctaaac
     9301 tcaggctggt attacgccgc gtcaaaccat ccagttgtac gatgccttta ttccgcatat
     9361 cgggataagt cggcatcaat ttagcaatcc cggtgccttt tttactggtt aactgcacta
     9421 tgtcaccgac agccagattt aacctggcca tatcggccgg atctaagcga gcataaccgc
     9481 gcccagcatc ctttggcaga gcctcggcga ccttgagctt gagttcttca gacatgactg
     9541 cctcattttc ctaatttgac ttccaaaatg ccattacggc aagaccgttc catcgccttg
     9601 tcatcgaatg agcacggcaa cacgatttct ttatgatatt ttttactgcc tctttccgag
     9661 tgtaacgtca gaatgtcgcc attcaattcg acttgcacat cttcatcagc gacccccggc
     9721 atttctgcca gtaccagcac atgatcactt tcttcgataa catcaaccaa cggttcagac
     9781 acttcctgca ctgtagcttc cccggtttgc tcatcccggc gtatgttgcc aaaaggttct
     9841 acccgatttt cctggtttcc atccaaaccc atcttgatgg agaaaccata cacggctttg
     9901 gcattcttac cgtcgttcgt gtcaatatcg aacgcaccgc tacgctttat ctgttcgccg
     9961 gtttcaacca atttttccac cagatcgccc aaaccgcgca aaattccttc aacattccct
    10021 gtcacatcat ttttttttta ctactcatga ttgtttacct cggctgcatt gatctttttg
    10081 agtttagagt gcagcgtttt atagtcaata ttcaaacgcc gcgctgcttc tgccttgtta
    10141 ttgcctgttt tctctaatgt gtcttgaatg ataattcgct ctatttgatc aatattgagt
    10201 tgagtgatct ctttaagaga cgaaccttcc agacagaaat tctgtatcgt tctcagatac
    10261 ggatttttcg ttgattgcgg aaagtgatct tctttgatga ccaggcctaa atcatcaacc
    10321 cctactcttt tgtcggatac caaaacagca cggcggatga cagcgcgtaa ctctcgcacg
    10381 ttacccggcc atggataacg taacagtgtt tctttagccg caacggacaa atcaggcggt
    10441 gttttgttca attcgacgct ggtttcggcg ataaaccgat tggacaaaaa cagaatatcc
    10501 tcgtgccgat tgcgtaacgg cggaatccga atgacatact cattcaatcg ataatagaga
    10561 tcctcgcgaa atttcccttt aactaccgcg tccagtagat catcattgct ggcagccagg
    10621 actcgcacat tgacaggaat gggtttagtc gcgccaatac gatagacaac gtgctcttgt
    10681 agcacacgca gtagcttggc ctgtgcggat aaagacatgt tggcgatttc atccaggaat
    10741 aacgtaccgc cgtcagccgc ttcaaactta cccgcatgaa cctgatcagc cccggtgtac
    10801 gagccttttt catgaccaaa tagttcattt tcgatcaacg agtcgggtat ggagccacaa
    10861 tcaatgggaa caaaaacccc tgtggcatgc tgactggcta aatggatgtt tttcgcaacc
    10921 aattctttgc cagttcccgt ttcaccctga ataacgacag aaaaatccgt atgagccacg
    10981 ttaacgacat ctctgatcac cgactgaatc tcgcggctat tccccatcat ggcagcgata
    11041 cgcgttttag tatccttgct ggctttgcag tttgaatcgt agttacccca acgcatttgc
    11101 attgcacgat tcaccagatc taatactcgt ttgttatcaa acggcttggg aatgtaatcc
    11161 catgcaccag ctttgatagc accaaccgcg ctaagaatgc cgacattgcc agtaataatg
    11221 ataacgggga gttgcggata cagagcatgg gcattggcta acacctccat gccactgtgt
    11281 ttagggagaa tactatcaag taacagtaca tccggttcac gctgagacaa caactttatt
    11341 gccatgtcgc catcgtaacc ttgctgagtt tcacaaccag ccttttccag caagtgtgat
    11401 agcaggtcac aaatatcttt gtcgtcatca attattaaaa catagccagg atcaatactt
    11461 ttcatcgact acatccaatt ggttatttca tttttaataa tagttaaaag atacggggaa
    11521 gaagaggata aatctccctt ctatcacgta tatatttgac gcaaaaaaag aattattaac
    11581 actctagtta tttttttaac acaaccgcct aatcatcgcc tagacggtgc ctaattttaa
    11641 tgaatagcat ccttaatact tatagtacaa atttaagata acacaatttt cttttcgatt
    11701 ttgtgacaat aatttaggca agtatttcta tttattaacg aatattttaa ctacccggtc
    11761 aaataagcga gagactttat tctggacatg ataaacatca tcccatcatc tgtctactga
    11821 tggctatcag ccatatcaag ggcgacactc cctttttagc cgattaggtc acatttttag
    11881 tatcaaaaga tcatagggct atcgataaaa ctggtaataa ctaagagaca tacatcacat
    11941 aaatagctaa taaaccaccg ccaaaaaacg tttgcctatg tcacatctgg catcaattcc
    12001 actagaagca ccatatatta tctaacaaaa taatgaatat tgatttcgat ttctttacac
    12061 aaaaatgaag gctgaaacag acaaacagga agggtagtat taacaaaatc atcccgtgat
    12121 ggaaattccg accacgggtt ccataatatt tatttatcac cgtatacgca ctatattgct
    12181 gctatgccga tacttttaca aacaaacata gttggttatt attacgtttt tcatcagtca
    12241 aaaaaacgca taaaaatata aaaaaagcaa caaagtccca ttattatgta gtctattaaa
    12301 atgacatata aattatattt atactacctc taatattctc ggtgaataaa tccacttcta
    12361 atgccaacga taatgcagaa caaggaatgt agtcactaaa tactttcccc tcctgggggt
    12421 ttacctaacg gttgaataaa aacaatatct gcgggcagct tcgcttcaat attgtgcata
    12481 agccccagga aaagtatcgc catctcgcca tcttgcagcg tttcatcaat gcgctgggca
    12541 atataatcat cccgccggtt caataatgtt tttgctgttt ccaaggacgc ttcggtttgc
    12601 tccgtagatt gtaacaactg cgtcatcaga tcgcgctctt gcaacaatag ttcaggggac
    12661 tctgttccca tcagaattgc gcctttatgt tgcaacgtaa gcaagagctt gtaattctgg
    12721 ctaccagcgt tagcaacatc ggtcactatt tcattttctt ttccacaaac cggcagacca
    12781 tcctgataaa gtttcagatg tgtatagtcc agatctaaac cctcaatcgt agtggtaatc
    12841 attgtccaga acccctcgac ggcagcaaga tacgcacgcg tttgagtttc ggttcgcaaa
    12901 tgacttgccg cttcgctcaa tgtccctaaa tctttagggc tatgaataat gggaaaatga
    12961 atcagcgctc gcataaaaaa gataatcctt ataaatgagt ttcttctatg acggacggtg
    13021 ccgcttccaa acgagcctct agccgctgca gacgttcttc caactctgga gcaggcgctg
    13081 atgatgacac tctggctctg gaatcaaacg ccggattgcc actccaccaa tccagcccca
    13141 tttctaccgc tttatctacg gaacagataa gcaggcgaat ttccagtgtc agcaattcga
    13201 cttcaaccag tctgatgcga atgtcaccgc tgatgacaac ccctttgtcc agtaatcgct
    13261 ctaacaaatc cgctacggtg gtcgaatcgg ttgaatgggt tagttttttg ttgccgctca
    13321 tggtgcaaat ccttataggg taaacaacct aaagacctca aatcctcatc cccttctctc
    13381 taaaatcaaa agcatatata cccaataaat ttcaacacac aggaaggcga caatcgagta
    13441 aatccccaag gcttactcca gtaagtgact ggggttagcg tgggcagaca acgcacctgc
    13501 aacttgaaag atgatctata ccgtactgat gctcttaatt cttggatcca gggtatcctg
    13561 aacatggatc tcactgcgta gaaaacactg ctggcgatat cgcttgcgac ttaatatagg
    13621 attcaaacct gataaatagg ccacgttaat atcctgaaac ttatatggat gtcggtgtta
    13681 tttccccaac gcgttcaagg atatcaagtg acagccggaa caaaccatct cgttcataac
    13741 gttgtgaaat ggtcaacagc gccgtttgcg cggttttact ctccttgctg tcaggatatt
    13801 gctcgattat tttcagatag atatccactg cttgtcgcag ttcatcttgt tcaaaccatg
    13861 cattacccat acgcagtaga agaccacgcg tagccgcatt tatgtcattg gtagtattaa
    13921 ccatggtaca caccctctgc cgaaaacaga ctgataacat ttatatggga actaaaaaat
    13981 aatcgtatgt tgttgttcat catacaattt ccttacagca atcggcctag cgggcctaaa
    14041 tccaagttca aatcatcatc cttgaagcca aaaacgtcgc acaaatgtgt caactcctcg
    14101 gcttggcgca ttagcgccaa acccaaacgc tcgacttctt catcactaag cgaacccgaa
    14161 tccatccgcc ttatggcttg acgctccagt agttcgtgca gcagtttgac cagggtcagg
    14221 acgagttgcg ccaaaccatc acggacgcgt tctccatcga tgtctatttt atgtcgatta
    14281 gcttcagtaa ttggccgttg tattgctggc gatgtcgggc cgaacacagg actatggtgc
    14341 gataactgat tggttgtcat agtgttactc cctaccgggt aaacggtttt tcgcttctac
    14401 agaagaaacc aaagcctgta ggcttaaata aatcaaatca atattagcga ctgaaatggt
    14461 gatatcagcg acaatcacca cacctttgtt cagaacgcgg tccaaggcct cacacagaga
    14521 gacctgttgc tgttcatcct gatactgttt gttcactggc ataatgtgta cgctcacagt
    14581 ttataaaaac aatgccgaac ccaatgtctt tagcttgctc aatcccgtag gttctgtttg
    14641 ccggaaaatc tgcgcgtgtg gttggctgtg gaacatctca tctgcgcact tcagctccaa
    14701 cgactctcga ctcgtgattg cctggcacag tgtgcaatcg ctgggaggtg taatctggtt
    14761 gataaacaac gcgtttgccg ttaagcctaa tcgctgcaat gcgcatgtca tttcataggt
    14821 cttctcaagt gccaaatgcg tcggaacagt aactgcatac aaccccgttt gtttggtatc
    14881 ctgcagtaga gcgcgtagat tttttaattc gcgtgaaagc tgtaccaaac gttccgataa
    14941 atgcgggaaa cgcatgactt tgcgatattt cagcaataac gagaaaaact gttttagcca
    15001 atcccgaatc aattcaggca actctaataa acgtagcaaa tgtccgctag gtgcgccatc
    15061 cacgatgacc atgtcataac gaccgctgtc tagatgatcc atgatcgcag tcaatgccat
    15121 gatttcatct aatccggggg gcgcgagatc gagcaaatgc tccaacactt cacgatcaaa
    15181 agtaatatcc agattgggta acgtatccag caagaaagct tctaattccg cacgatatcc
    15241 ctgccggatt ttgtcaaaat cagcttgtgc attaatttcc tgggcatcaa tgttaaccaa
    15301 caccgatata ggctgctgct gtagggttac acccagacaa tcactcagcg agtgagcagg
    15361 atcggctgag aacaacagaa tacgcaattc tggatattca ctgttcagac gcaatgccgt
    15421 agcacaggcc agcgtcgttt tacctacccc acctttacct gcaaaaatca gcagtcggaa
    15481 tgtgctggca ggcagcgaaa tggaactctc tacacgtaaa ggcagttgat gatgacacgt
    15541 cgtagccatg acttcgtttt catcaagtaa acggaccccg gaccaaaatt cgtgaagcaa
    15601 cgcaccacgc ggttcgatgg caagcaaagg caacgtccag aatacgtgcc caggcaaacg
    15661 ttggtatccg ttctggagcg caagcatttg tctgttacgc tcaacacaac aggttggaca
    15721 gtcattttca ggaaatagtc gattgaccac taaatcggac agaaacaccc gttgctgatt
    15781 caacgcgcca gcaagatcaa tgctttcttc cacactcatc gcttccgcca gcatcactaa
    15841 cacaaaacaa caacgcgtgg aatccgtcac cagctcgtgc atagctttaa gtgaatcatt
    15901 catgtccagc agaaaatgat ccagatgatc caatcgatta tcaccggcaa aacgtttccg
    15961 gatataacgc tgtttggcaa gcagggtatc cagagcggtc aaccaccgat aaatcaaatc
    16021 cggcatttcc aaaagccgca atgtatgacc tgtaggagca gtatcaataa taatacgata
    16081 gtaagtatct ttttgaatcc attcagctat ttcaagataa gcggccaatt catccatgcc
    16141 gggaagcgcg gtatccatca atccttgtac gtcgttttga tcgagaacag tgccgcgata
    16201 cgctatctct ttcagaacac cttcatgctg cgatttaaat tcatgcaagg aggctgcggc
    16261 atttaattca cgtacgtcca gattttttgg caataccaga tcggagagaa tattttgcag
    16321 ggaatgtgct ggatcggtag aaaccagcaa aaaatggtgc tgcggctgtt cctgcgccag
    16381 ccttaacgcc gtcgacgtcg cacaggtact tttacctact cctcccttac cgccgaagaa
    16441 aaccagtttc agcggcgact ccagtaagaa cttgggataa atagcaggtt tcatttcgtc
    16501 gtaccctctg gctcatcctc gtcgtcgtca tcctcgtcgt cgtcgtcatc ctcttcgtcg
    16561 tcgtcatcct cgtcgtcgtc atcctcgtcg tcgtcatcct cttcgtcgtc gtcctcttcg
    16621 tcgtcgtcct cttcgtattc gtcgtcttcg tcatccccag gctcgtcgcc tagcatgtca
    16681 tcttcttcat ccagtgcgtc taatctatcc agcagtacag cttcctgttg ttcaaattca
    16741 tcttcggtaa tctggccagt ctccagtaac atataaagat cagtcaaact ttcacgaata
    16801 cgatccgcct caccagccaa ttcatcttcc gccagttcgt gaatttgccg aaaaatccac
    16861 atgacgcctt tgacaggaga aaacaggatg tcatcgatca gcagcatggc ttagcctcag
    16921 tcagagagag taccagcgcg ggtcaacgtt ttcttcttcg ctgtaggagc atgtaaatcc
    16981 agagtaacga aattatgcgg tgcccatggg ccggtataat cgaacagata aacgttatcg
    17041 aataactttg atgcctcaaa aacgcctttg gcaaattcat ccatccgttc tcttgctacc
    17101 aaacaggcta gattcattac gtctttttct tttttaaccg ggttttcaat aatctcttcg
    17161 caataatcca acagaacttc ttttactttt tccgtcgatt cttttctgtc tccactacgc
    17221 aatgactcgt acaaattacc cagccgaatc ttctcttcac ggtgattatt ggcattggaa
    17281 tttccattcc agatttcatc gcgcgtttca cttaagactg gatatgtagc aacaaaatat
    17341 tcataaatat tagtaacatc ccaggaaaca cgtagcccca tttccacaca gccatccaag
    17401 cgttcaaaat gttctctgat cgtatcctga ttagcgacca gcaaattctt tactgcttca
    17461 gcattacggg caataacccc aaagcgcatc ggcaatacgg taccttcttc agtcaactta
    17521 tgtaatacag cctgatgcgc cgtgatatta cgtctttctg gacgtaaccg ggtactgagc
    17581 gtatcgctga ccacagctct taagccatct tgatcaatag aataaaccgg tgctgcttcc
    17641 aatccgcgta ttttatagtt aggcgttgag tcatcggaaa cacagagcgc ataaagcacc
    17701 ttggcacgat gatttctgga tttatcaata ctcatcatgc actcctcaat gaaatacgtt
    17761 tacttttggc gcattgcaca cggttcaatc gtcgatattc ttctaaataa atacgtccaa
    17821 aatcagcgag aatttgtgac ttccccgtca tgttgacgca tgtgcgaaag ctcttcacca
    17881 tacgcggtgt acggatatga gcgagggtgc gaattttgtt cataatgaca acctcaatac
    17941 ttgattcgca aaccagaaga ctttttcttc ttctcggccg cttctgattt atcattattt
    18001 tcagccacag cactcgccgc tgtcgcgtct gcaagcagcg ctgctttttc tttatcaata
    18061 tccgcgatac gctcatcaat actggcaata cgcgatgagg cagcctcacg ttctcgtgtt
    18121 cgccgccaac gctctaattc cagcgcacct atctgaaaat aatcacgata ggtttgatgc
    18181 tcaccactca ctcgacctga atgagttttt atgtctgaca gtgttctgag aggacgagta
    18241 cttatagcca tgataattac tccactgctt ttattttttg tgtggatgct ttaaccgtgt
    18301 ctgaacctat tggtggacag attttacgaa taacgccatc gaccatttca cggtatatag
    18361 aacgtccagc gtgttgtatt ttggcagcgt ccatactcaa gacgtcgtag cagatagcat
    18421 ggaataattt gtcaccataa cgtggtttta ttccttgctg gtgtaggatg tgggcgatgg
    18481 caattcctgc acgtaaccca tgtttggttt cgtgttcgtt accacgcaac tctctgatta
    18541 tatcaacgat atatgccgct tcttttaatt ccaattcaga gcgcgcatgc agtatctgta
    18601 gttcagtatc ccgatcgaca agaccaatgt tgatagtaat catacgatcc atcagagcgt
    18661 cctgggtctt atgcgtaccg gcatactctt ctggattgga ggtaaaaata gcgcgaaatt
    18721 ctgggtgtac atcgagatac ccttccccta caccacgtaa accaggaagg tttaaaatcc
    18781 cttcggataa tacggataac agcacattat tggtttctgc tttggaacga ttgaactcat
    18841 catagatcag catgtcgccg ttacgacacg cggtggtcaa acggttatcc acccacatgc
    18901 ggttcatctg ttcttcagtt ttaagcactg aatgaatgta gttatccacc aggctgcttt
    18961 tacgatagcc gatgtctttc cccgtcaaat cggagctcac aaattcttcg ttaccttgca
    19021 acatggtaac gggacgaccc catagtgctg cgagatgaaa tgccagggtt gttttcccta
    19081 tcccagatgg gccggcaaaa tgcacgggat agccagcgcg taaataaacc aatgcgcgtt
    19141 ctataatgtc gttcacataa gaagtcgcta caaaatgttc actcgcttcc ggaacgacaa
    19201 gatcatcgtc tacggtatac tgcgaaacgg tattttgctt aatcatggaa atactcctgt
    19261 gccaaaccgt gtacgcccta gccaacacca gttctacgaa atggccaaat cagtgatcgt
    19321 cttagccgct agaatcggtc gattttagcg gattcttata tttcgattta acttagcgat
    19381 taatcgaaac tgtatgccca ataatgtcac tccgtgtttg tgagcagact gttcatccac
    19441 ctcaccctcc atggacgaca gaggctaata ccgctactta ccttttttca tcttcactac
    19501 ttaccgccag agtattatca agaggacgag tggctagact gcgaggctcg tttttttttt
    19561 agccagggtc atgccgaaaa atacagcatg ggcagccatt ctgtccttct cagaagcact
    19621 aagaaaagca gcgacatcgt ttgccacgtt ggcaatgaaa tcagctcgtt ctttagcaca
    19681 gcgttcagcc atacttttac gctcagcatt aaaaccgtct aataaagcta gcgttttctt
    19741 cgacatatcc gtcacgaagg tggcacgttc ttcactctgt tgacgactca ttacctcgcg
    19801 ggtatgacag aagtcactca acatccgatt aacgcctctg acattatcgg caacgaatgt
    19861 cgcgcgtgcg cgtgcgtctt gagccgcgtt tctagcccga gttgtagaaa aaccggcaat
    19921 ctgcgcactc acactggaaa cccgcgcatt ttgctgtgct atgcgagatt catgactgtc
    19981 atcaatattc ttacgcagct gcgccatacc gtctgttaag catcccatag gattctcctt
    20041 atgtgataga gcactcttga agcgttgaaa agtactcaag cagctgaatg actcagccac
    20101 ttgggcactt ttattctgct tacttaatta cgctggagtc gctgcactgg ctgtaaggcc
    20161 aatcgcttct gcgtatttca aataagtttc aacagaagca ataacaacac gtgcttcgat
    20221 ggataacagt tcaataccaa ccaatgagac tttgacccaa gcgtcaatca caatgccttt
    20281 gtccagaata cggtctacta cttcagccaa gctggaagag tctgttgatt tttgtacttt
    20341 agccatgatg gcgtctccta gaaaatgtta ataaattaca tatgtaatta ggttctacgt
    20401 acagacttgt tactgcacgc ccatgtagaa tgcaactcat gcgccaacct ataggcatca
    20461 gcgctctatt aatgttaata aatccaccac aatgaaggtt aattcctcct gttagcccaa
    20521 aaaaacgggt atggagaaac agtagagagt tgcgataaaa agcgtcaggt aggatccgct
    20581 aatcttatgg ataaaaatgc tatggcatag caaagtgtga cgccgtgcaa ataatcaatg
    20641 tggacttttc tgccgtgatt atagacactt ttgttacgcg tttttgtcat ggctttggtc
    20701 ccgctttgtt acagaatgct tttaataagc ggggttaccg gtttggttag cgagaagagc
    20761 cagtaaaaga cgcagtgacg gcaatgtctg atgcaatatg gacaattggt ttcttctctg
    20821 aatggcggga gtatgaaaag tatggctgaa gcgcaaaatg atcccctgct gccgggatac
    20881 tcgtttaatg cccatctggt ggcgggttta acgccgattg aggccaacgg ttatctcgat
    20941 ttttttatcg accgaccgct gggaatgaaa ggttatattc tcaatctcac cattcgcggt
    21001 cagggggtgg tgaaaaatca gggacgagaa tttgtttgcc gaccgggtga tattttgctg
    21061 ttcccgcaag gagagattca tcactacggt cgtcatccgg aggctcgcga atggtatcac
    21121 cagtgggttt actttcgtcc gcgcgcctac tggcatgaat ggcttaactg gccgtcaata
    21181 tttgccaata cggggttctt tcgcccggat gaagcgcacc agccgcattt cagcgacctg
    21241 tttgggcaaa tcattaacgc cgggcaaggg gaagggcgct attcggagct gctggcgata
    21301 aatctgcttg agcaattgtt actgcggcgc atggaagcga ttaacgagtc gctccatcca
    21361 ccgatggata atcgggtacg cgaggcttgt cagtacatca gcgatcacct ggcagacagc
    21421 aattttgata tcgccagcgt cgcacagcat gtttgcttgt cgccgtcgcg tctgtcacat
    21481 cttttccgcc agcagttagg gattagcgtc ttaagctggc gcgaggacca acgtatcagc
    21541 caggcgaagc tgcttttgag caccacccgg atgcctatcg ccaccgtcgg tcgcaatgtt
    21601 ggttttgacg atcaactcta tttctcgcgg gtatttaaaa aatgcaccgg ggccagcccg
    21661 agcgagttcc gtgccggttg tgaagaaaaa gtgaatgatg tagccgtcaa gttgtcataa
    21721 ttggtaacga atcagacaat tgacggcttg acggagtagc atagggtttg cagaatccct
    21781 gcttcgtcca tttgacaggc acattatgca tcgatgataa gctgtcaaac atgagcagat
    21841 catctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc
    21901 ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcaa
    21961 atccgctaat taactaaaac tagtacaaaa aatttaaaat agattattaa acctattgct
    22021 tattctattc agtcatatat ataagcgtca tcctttagta actaacatcc cacactacaa
    22081 tagtattata acatatctat tcaacttcaa attgttgaaa tttaagccat ttatatttac
    22141 ttttcctcaa ctttagaaat gattttacta accttatatc cgtaatacgc gtaacaaaca
    22201 cattcattaa taacaatcaa cactaagcta tattagcttt ttaaatgact agggtaaata
    22261 aaataaggat caaaaacttt taagtttctg accctttcct tacttccgat tggttaatag
    22321 tgatcttttg cagaataaat aaatatcgtt tcattttcaa ctctatatat cagtctatgt
    22381 tcatctgtaa ttcttctgga ccattttcca gataaatcat gctttaatgg ctcaggtttt
    22441 cctaatccag caaaggggga acgatcgata tcttttatta acttgttaat cttttttata
    22501 ttgcttttgt ttccttgctc atgccaataa agataatcat cccaagcatc atcagaccaa
    22561 gccttaatca tcagattcaa cctcgattaa gtcatgtgtt ttaaatgcac ctttggagaa
    22621 ttgttcatct cctcgacgaa ttttttccat gacgtaatta ttagaaagtg ttctcaacgt
    22681 ttcttgcata gaatcataat ctctttttga taatacaaca actgtatctt ctacatcttt
    22741 acttgttaca ataagtgttt cagcatcctc attaacttgt ttcatataac tacgtaaatt
    22801 ttggcggaaa tttgaataag ctactgcttc cattcctttc accccttatt tatttctata
    22861 aaacaattgt acattatatt gtacaattaa gcaaatgatt aatattctaa cttcttataa
    22921 ttaaaagtgc tatcactttt agtttttagg ataaaaagtg acgtcacttt ttgagtgctg
    22981 gtacctcaaa tcctgctttt tcttgggcaa tcaataaatc taacgcaata cacataatcc
    23041 tggattcact tttgccgtac ttctgcttta gttgctgcaa ttctttttgt ctcttttcat
    23101 ccaacgtaac gggtaaccgt ttatttccta ttgcaattgg cattagtact ctctcctttc
    23161 atcgaaataa ccctatttta tctgattaat aagatgatct tc
//