FHRE-Luc

LOCUS       FHRE-Luc                5094 bp DNA     circular SYN 13-JUL-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5094)
  AUTHORS   Biofeng
  TITLE     Direct Submission
  JOURNAL   Exported Jul 13, 2023 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..5094
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..328
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      179..314
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      358..367
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             364..2016
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    2057..2178
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2597..3185)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3356..4216)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4217..4321)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4348..4803
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     polyA_signal    4934..4982
                     /note="synthetic polyadenylation signal"
     misc_feature    4996..5087
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcgtga cagagtgaga ctctgtctct atttaaataa
       61 ataagtaaat aaataaactg ggcaaacaat gaaaatgaaa acattgtgaa atacaaagca
      121 gatctgcgat ctgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
      181 cccgccccta actccgccca gttccgccca ttctccgccc catcgctgac taattttttt
      241 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
      301 cttttttgga ggcctaggct tttgcaaaaa gcttggcatt ccggtactgt tggtaaagcc
      361 accatggaag acgccaaaaa cataaagaaa ggcccggcgc cattctatcc gctggaagat
      421 ggaaccgctg gagagcaact gcataaggct atgaagagat acgccctggt tcctggaaca
      481 attgctttta cagatgcaca tatcgaggtg gacatcactt acgctgagta cttcgaaatg
      541 tccgttcggt tggcagaagc tatgaaacga tatgggctga atacaaatca cagaatcgtc
      601 gtatgcagtg aaaactctct tcaattcttt atgccggtgt tgggcgcgtt atttatcgga
      661 gttgcagttg cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa cagtatgggc
      721 atttcgcagc ctaccgtggt gttcgtttcc aaaaaggggt tgcaaaaaat tttgaacgtg
      781 caaaaaaagc tcccaatcat ccaaaaaatt attatcatgg attctaaaac ggattaccag
      841 ggatttcagt cgatgtacac gttcgtcaca tctcatctac ctcccggttt taatgaatac
      901 gattttgtgc cagagtcctt cgatagggac aagacaattg cactgatcat gaactcctct
      961 ggatctactg gtctgcctaa aggtgtcgct ctgcctcata gaactgcctg cgtgagattc
     1021 tcgcatgcca gagatcctat ttttggcaat caaatcattc cggatactgc gattttaagt
     1081 gttgttccat tccatcacgg ttttggaatg tttactacac tcggatattt gatatgtgga
     1141 tttcgagtcg tcttaatgta tagatttgaa gaagagctgt ttctgaggag ccttcaggat
     1201 tacaagattc aaagtgcgct gctggtgcca accctattct ccttcttcgc caaaagcact
     1261 ctgattgaca aatacgattt atctaattta cacgaaattg cttctggtgg cgctcccctc
     1321 tctaaggaag tcggggaagc ggttgccaag aggttccatc tgccaggtat caggcaagga
     1381 tatgggctca ctgagactac atcagctatt ctgattacac ccgaggggga tgataaaccg
     1441 ggcgcggtcg gtaaagttgt tccatttttt gaagcgaagg ttgtggatct ggataccggg
     1501 aaaacgctgg gcgttaatca aagaggcgaa ctgtgtgtga gaggtcctat gattatgtcc
     1561 ggttatgtaa acaatccgga agcgaccaac gccttgattg acaaggatgg atggctacat
     1621 tctggagaca tagcttactg ggacgaagac gaacacttct tcatcgttga ccgcctgaag
     1681 tctctgatta agtacaaagg ctatcaggtg gctcccgctg aattggaatc catcttgctc
     1741 caacacccca acatcttcga cgcaggtgtc gcaggtcttc ccgacgatga cgccggtgaa
     1801 cttcccgccg ccgttgttgt tttggagcac ggaaagacga tgacggaaaa agagatcgtg
     1861 gattacgtcg ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt tgtgtttgtg
     1921 gacgaagtac cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag agagatcctc
     1981 ataaaggcca agaagggcgg aaagatcgcc gtgtaattct agagtcgggg cggccggccg
     2041 cttcgagcag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     2101 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     2161 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     2221 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatcgataag
     2281 gatccgtcga ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg
     2341 gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca
     2401 ggtgccggca gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     2461 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     2521 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     2581 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     2641 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     2701 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     2761 ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg
     2821 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     2881 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     2941 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     3001 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     3061 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     3121 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     3181 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     3241 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     3301 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     3361 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     3421 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     3481 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     3541 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     3601 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     3661 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     3721 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     3781 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     3841 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     3901 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     3961 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     4021 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     4081 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     4141 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     4201 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     4261 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     4321 gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta agcgcggcgg
     4381 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     4441 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     4501 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     4561 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     4621 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     4681 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     4741 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa
     4801 tttcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     4861 cgctattacg ccagcccaag ctaccatgat aagtaagtaa tattaaggta cgggaggtac
     4921 ttggagcggc cgcaataaaa tatctttatt ttcattacat ctgtgtgttg gttttttgtg
     4981 tgaatcgata gtactaacat acgctctcca tcaaaacaaa acgaaacaaa acaaactagc
     5041 aaaataggct gtccccagtg caagtgcagg tgccagaaca tttctctatc gata
//