| 质粒名称: | pDJ655 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001154 |
| 产品用途: | mutiplexed signaling recording for NFAT and mTOR |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | mTOR-responsive, NFAT-responsive |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | mGreenLantern-iPAK4, Electra1-iPAK4 |
| 物种来源: |
Synthetic
|
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Fluorescent protein-based ticker tapes for multiplexed recordings of transcriptional histories in single cells in culture and in vivo
Wang R., Jiang J., Li Z., Liu T., Wang Y., Xie M., Piatkevich K.D.
Preprint
|
pDJ655
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pDJ655载体质粒基本信息
pDJ655质粒图谱载体图谱和pDJ655载体序列质粒序列多克隆位点信息
pDJ655质粒载体简介
pDJ655质粒序列载体序列
LOCUS pDJ655 8315 bp DNA circular SYN 10-MAR-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8315)
AUTHORS Westlake University
TITLE Direct Submission
JOURNAL Exported Aug 5, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8315
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 1..20
/label=oSCL812
misc_feature complement(84..115)
/label=SleepingBeauty-ITR_Li
/label=nonstandard type: ITR
misc_feature complement(281..311)
/label=SleepingBeauty-ITR_Lo
/label=nonstandard type: ITR
promoter 432..536
/label=AmpR promoter
misc_feature 537..1394
/label=AmpR
/label=nonstandard type: resistance
rep_origin 1492..2174
/label=ColE1 origin
misc_feature complement(2344..2375)
/label=SleepingBeauty-ITR-Ro
/label=nonstandard type: ITR
misc_feature 2541..2569
/label=SleepingBeauty-ITR_Ri
/label=nonstandard type: ITR
primer_bind complement(2635..2673)
/label=oSCL807
misc_feature complement(2654..2673)
/label=P_RPBSA
/label=nonstandard type: promoter_const
primer_bind 2654..2673
/label=oSCL808
primer_bind complement(2654..2673)
/label=oSCL807a
primer_bind 2670..2711
/label=oDJ655-1
misc_feature 2688
/label=CMV
misc_feature 2709..2726
/label=NFAT_IL4
misc_feature 2739..2756
/label=NFAT_IL4
misc_feature 2769..2786
/label=NFAT_IL4
misc_feature 2799..2816
/label=NFAT_IL4
misc_feature 2829..2846
/label=NFAT_IL4
promoter 2860..2891
/label=minP
/note="minimal TATA-box promoter with low basal activity"
primer_bind 2897..2944
/label=NFAT-EcoRI-mGL-F
regulatory 2924..2933
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 2930..3646
/codon_start=1
/label=mGreenLantern
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
KFICTTGKLPVPWPTLVTTLGYGVACFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
GTYKTRAEVKFEGDTLVNRIVLKGIDFKEDGNILGHKLEYNFNSHKVYITADKQKNGIK
ANFKTRHNVEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLK
ERVTAAGITHDMDELYK"
primer_bind complement(3631..3672)
/label=INKA-mGreenLantern-R
CDS 3659..3772
/codon_start=1
/label=INKA-box
/translation="EAEDWTAALLNRGRSRQPLVLGDNCFADLVHNWMELPE"
CDS 3779..4720
/codon_start=1
/label=Pak4cat
/translation="PAAPAVPGPPGPRSPQREPQRVSHEQFRAALQLVVDPGDPRSYLD
NFIKIGEGSTGIVCIATVRSSGKLVAVKKMDLRKQQRRELLFNEVVIMRDYQHENVVEM
YNSYLVGDELWVVMEFLEGGALTDIVTHTRMNEEQIAAVCLAVLQALSVLHAQGVIHRD
IKSDSILLTHDGRVKLSDFGFCAQVSKEVPRRKSLVGTPYWMAPELISRLPYGPEVDIW
SLGIMVIEMVDGEPPYFNEPPLKAMKMIRDNLPPRLKNLHKVSPSLKGFLDRLLVRDPA
QRATAAELLKHPFLAKAGPPASIVPLMRQNRTR"
primer_bind complement(3872..3890)
/label=PAK4-R
primer_bind complement(4610..4619)
/label=oSCL808
primer_bind complement(4700..4746)
/label=XmaI-PAK4-R
primer_bind complement(4703..4723)
/label=HindIII-PAK4-R
primer_bind complement(4703..4723)
/label=oDJ655-4
primer_bind complement(4704..4723)
/label=oDJ580-2
primer_bind 4764..4780
/label=oSCL815
polyA_signal 4773..4894
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4876..4894)
/label=SV40-POLYa
primer_bind complement(4959..4983)
/label=oSCL816
primer_bind complement(4990..5024)
/label=oDJ655-2
primer_bind 5005..5034
/label=oDJ655-3
primer_bind 5015..5034
/label=oDJ580-1
misc_feature 5036..5045
/label=SRE(mTOR)
misc_feature 5057..5066
/label=SRE(mTOR)
misc_feature 5078..5087
/label=SRE(mTOR)
misc_feature 5093..5100
/label=CRE
misc_feature 5121..5129
/label=E-Box
misc_feature 5143..5151
/label=E-Box
promoter 5181..5218
/label=minimal CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 5315..5357
/label=XbaI-Electra1-F
regulatory 5337..5346
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 5343..6053
/codon_start=1
/label=Calypso1D6
/translation="MVSKGEELIEENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTM
RIKVIEGGPLPFAFDILATSFLFGSKTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGG
VVTVTQDTSLEDGGLVYNVKVRGVNFPSNGPVMQKKTEGWEPFTEMMYPANGGLRGYTD
IALKVDGDGHLHANIVTTYRSKKTVGDIKMPGVHAVDYRLERVEESDNETYVVLREVAV
AKYSNLGGGMDELYK"
primer_bind complement(5620..5656)
/label=Qch-R
primer_bind 5620..5656
/label=Qch-F
primer_bind complement(6038..6079)
/label=INKA-mGreenLantern-R
CDS 6066..6179
/codon_start=1
/label=INKA-box
/translation="EAEDWTAALLNRGRSRQPLVLGDNCFADLVHNWMELPE"
CDS 6186..7127
/codon_start=1
/label=Pak4cat
/translation="PAAPAVPGPPGPRSPQREPQRVSHEQFRAALQLVVDPGDPRSYLD
NFIKIGEGSTGIVCIATVRSSGKLVAVKKMDLRKQQRRELLFNEVVIMRDYQHENVVEM
YNSYLVGDELWVVMEFLEGGALTDIVTHTRMNEEQIAAVCLAVLQALSVLHAQGVIHRD
IKSDSILLTHDGRVKLSDFGFCAQVSKEVPRRKSLVGTPYWMAPELISRLPYGPEVDIW
SLGIMVIEMVDGEPPYFNEPPLKAMKMIRDNLPPRLKNLHKVSPSLKGFLDRLLVRDPA
QRATAAELLKHPFLAKAGPPASIVPLMRQNRTR"
primer_bind complement(6279..6297)
/label=PAK4-R
primer_bind complement(7017..7026)
/label=oSCL808
primer_bind complement(7107..7130)
/label=XmaI-PAK4-R
primer_bind complement(7110..7150)
/label=oDJ655-4
primer_bind complement(7110..7130)
/label=HindIII-PAK4-R
primer_bind complement(7111..7130)
/label=oDJ580-2
terminator 7165..7392
/label=bGH PA
primer_bind complement(7374..7415)
/label=oSCL809
primer_bind 7396..7432
/label=oSCL841
misc_feature 7416..7624
/label=pSV40
primer_bind complement(7606..7646)
/label=oSCL844
primer_bind complement(7606..7643)
/label=oSCL842
primer_bind 7627..7681
/label=oSCL813
primer_bind 7627..7667
/label=oSCL818
promoter 7627..7631
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind 7647..7681
/label=oCW11
primer_bind 7657..7681
/label=oSCL517
misc_feature 7668..8060
/label=BlastR
/label=nonstandard type: resistance
primer_bind complement(7727..7765)
/label=oCW13
primer_bind 7746..7777
/label=oCW14
primer_bind 7958..7968
/label=oSCL841
primer_bind complement(8043..8089)
/label=oCW12
primer_bind complement(8043..8089)
/label=oCW15
primer_bind complement(8044..8069)
/label=oSCL516
primer_bind 8070..8112
/label=oSCL815
polyA_signal 8096..8287
/label=SV40 late polyA
primer_bind complement(8208..8226)
/label=SV40-POLYa
primer_bind complement(join(8291..8315,1..20))
/label=oSCL816
ORIGIN
1 cttctgaggc ggaaagaacc agctgggcct actagatagc ggacccctta ccgaaacatc
61 gccgcattct gcagaggagt cgaacttagt gtatgtaaac ttctgaccca ctggaattgt
121 gatacagtga attataagtg aaataatctg tctgtaaaca attgttggaa aaatgacttg
181 tgtcatgcac aaagtagatg tcctaactga cttgccaaaa ctattgtttg ttaacaagaa
241 atttgtggag tagttgaaaa acgagtttta atgactccaa cttaagtgta tgtaaacttc
301 cgacttcaac tgtataggga tccaatttta acaaaatatt aacgcttaca atttcctgat
361 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatcaggt ggcacttttc
421 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
481 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
541 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
601 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
661 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
721 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
781 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
841 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
901 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
961 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
1021 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
1081 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
1141 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
1201 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
1261 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
1321 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
1381 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
1441 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
1501 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
1561 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
1621 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
1681 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
1741 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
1801 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
1861 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
1921 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
1981 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
2041 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
2101 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
2161 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
2221 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
2281 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggatccct
2341 atacagttga agtcggaagt ttacatacac cttagccaaa tacatttaaa ctcacttttt
2401 cacaattcct gacatttaat cctagtaaaa attccctgtc ttaggtcagt taggatcacc
2461 actttatttt aagaatgtga aatatcagaa taatagtaga gagaatgatt catttcagct
2521 tttatttctt tcatcacatt cccagtgggt cagaagttta catacactcg actcctctgc
2581 agaatgcggc gatgtttcgg taaggggtcc cgagtagcta gttcatggca gccagcatat
2641 ggcatatgtt gccttttgtg agacagggtc tcgggccaga tatacgcgtg gtacctgagc
2701 tcgctagcta cattggaaaa ttttatacac gttctagcta cattggaaaa ttttatacac
2761 gttctagcta cattggaaaa ttttatacac gttctagcta cattggaaaa ttttatacac
2821 gttctagcta cattggaaaa ttttatacac gttagactct agagggtata taatggaagc
2881 tcgacttcca gcttggcaat ccggtactgt tggtaaagaa ttcgccacca tggtgagcaa
2941 gggcgaggag ctgttcaccg gcgtggtgcc tatcctggtg gagctggacg gcgacgtgaa
3001 cggccacaag ttcagcgtga ggggcgaggg cgagggcgac gccaccaacg gcaagctgac
3061 cctgaagttc atctgcacca ccggcaagct gcctgtgcct tggcctaccc tggtgaccac
3121 cctgggctac ggcgtggcct gcttcgccag gtaccctgac cacatgaagc agcacgactt
3181 cttcaagagc gccatgcctg agggctacgt gcaggagagg accatcagct tcaaggacga
3241 cggcacctac aagaccaggg ccgaggtgaa gttcgagggc gacaccctgg tgaacaggat
3301 cgtgctgaag ggcatcgact tcaaggagga cggcaacatc ctgggccaca agctggagta
3361 caacttcaac agccacaagg tgtacatcac cgccgacaag cagaagaacg gcatcaaggc
3421 caacttcaag accaggcaca acgtggagga cggcggcgtg cagctggccg accactacca
3481 gcagaacacc cctatcggcg acggccctgt gctgctgcct gacaaccact acctgagcca
3541 ccagagcaag ctgagcaagg accctaacga gaagagggac cacatggtgc tgaaggagag
3601 ggtgaccgcc gccggcatca cccacgacat ggacgagctg tacaagtcag gtggatccga
3661 agcagaggac tggacagcag ccctactgaa caggggtcgc agtcgccagc ccctggtact
3721 aggggacaat tgctttgctg acttggtgca caactggatg gagctgcctg aggaattccc
3781 cgccgcccct gctgttcctg ggccccctgg cccccgctca ccacagcggg agccacagcg
3841 agtatcccat gagcagttcc gggctgccct gcagctggtg gtggacccag gcgacccccg
3901 ctcctacctg gacaacttca tcaagattgg cgagggctcc acgggcatcg tgtgcatcgc
3961 caccgtgcgc agctcgggca agctggtggc cgtcaagaag atggacctgc gcaagcagca
4021 gaggcgcgag ctgctcttca acgaggtggt aatcatgagg gactaccagc acgagaatgt
4081 ggtggagatg tacaacagct acctggtggg ggacgagctc tgggtggtca tggagttcct
4141 ggaaggaggc gccctcaccg acatcgtcac ccacaccagg atgaacgagg agcagatcgc
4201 agccgtgtgc cttgcagtgc tgcaggccct gtcggtgctc cacgcccagg gcgtcatcca
4261 ccgggacatc aagagcgact cgatcctgct gacccatgat ggcagggtga agctgtcaga
4321 ctttgggttc tgcgcccagg tgagcaagga agtgccccga aggaagtcgc tggtcggcac
4381 gccctactgg atggccccag agctcatctc ccgccttccc tacgggccag aggtagacat
4441 ctggtcgctg gggataatgg tgattgagat ggtggacgga gagcccccct acttcaacga
4501 gccacccctc aaagccatga agatgattcg ggacaacctg ccaccccgac tgaagaacct
4561 gcacaaggtg tcgccatccc tgaagggctt cctggaccgc ctgctggtgc gagaccctgc
4621 ccagcgggcc acggcagccg agctgctgaa gcacccattc ctggccaagg cagggccgcc
4681 tgccagcatc gtgcccctca tgcgccagaa ccgcaccaga tgacccgggt tactctagag
4741 tcggggcggc cggccgcttc gagcagacat gataagatac attgatgagt ttggacaaac
4801 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
4861 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
4921 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
4981 tggtaaaatc gataaggatc cgtttgcgta ttggccgaaa agtgccacct gacgtatcac
5041 cccacacatc tgacgtatca ccccacacat ctgacgtatc accccacaca tctgacgtca
5101 ggatgtcctg caggacatct atcacgtgac tgcaggacat ctatcacgtg actgcaggtc
5161 gagctcggta cccgggtcga gtaggcgtgt acggtgggag gcctatataa gcagagctcg
5221 tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag
5281 acaccgggac cgatccagcc tccgcggaat tcgagctcgc ccggggatcc tctagagcca
5341 ccatggtgtc taagggcgaa gagctgatcg aggaaaatat gcgtatgaag gtggtcatgg
5401 aaggttcggt caacggccac caattcaaat gcacaggtga aggagaaggc agaccgtacg
5461 agggagtgca aaccatgagg atcaaagtca tcgagggagg acccctgcca tttgcctttg
5521 acattcttgc cacgtcgttc ctgttcggca gcaagacctt tatcaagtac ccggccgaca
5581 tccctgattt cttcaaacag tcctttcctg agggctttac ttgggaaaga gttacgagat
5641 acgaagatgg tggagtcgtc accgtcacgc aggacaccag ccttgaggat ggggggctcg
5701 tctacaacgt caaggtcaga ggggtaaact ttccctccaa tggtcccgtg atgcagaaga
5761 agaccgaggg ttgggagcct ttcacagaga tgatgtatcc agcaaatggt ggtctgagag
5821 gatataccga catcgcactg aaagttgatg gtgatggcca tctgcacgcc aacatcgtga
5881 caacttacag gtcaaaaaag accgtcgggg acatcaagat gcccggtgtc catgccgttg
5941 attaccgcct ggaaagggtc gaagagagtg acaatgaaac ctacgtagtg ctaagagaag
6001 tggcagttgc caaatacagc aaccttggtg gtggcatgga cgagctgtac aagtcaggtg
6061 gatccgaagc agaggactgg acagcagccc tactgaacag gggtcgcagt cgccagcccc
6121 tggtactagg ggacaattgc tttgctgact tggtgcacaa ctggatggag ctgcctgagg
6181 aattccccgc cgcccctgct gttcctgggc cccctggccc ccgctcacca cagcgggagc
6241 cacagcgagt atcccatgag cagttccggg ctgccctgca gctggtggtg gacccaggcg
6301 acccccgctc ctacctggac aacttcatca agattggcga gggctccacg ggcatcgtgt
6361 gcatcgccac cgtgcgcagc tcgggcaagc tggtggccgt caagaagatg gacctgcgca
6421 agcagcagag gcgcgagctg ctcttcaacg aggtggtaat catgagggac taccagcacg
6481 agaatgtggt ggagatgtac aacagctacc tggtggggga cgagctctgg gtggtcatgg
6541 agttcctgga aggaggcgcc ctcaccgaca tcgtcaccca caccaggatg aacgaggagc
6601 agatcgcagc cgtgtgcctt gcagtgctgc aggccctgtc ggtgctccac gcccagggcg
6661 tcatccaccg ggacatcaag agcgactcga tcctgctgac ccatgatggc agggtgaagc
6721 tgtcagactt tgggttctgc gcccaggtga gcaaggaagt gccccgaagg aagtcgctgg
6781 tcggcacgcc ctactggatg gccccagagc tcatctcccg ccttccctac gggccagagg
6841 tagacatctg gtcgctgggg ataatggtga ttgagatggt ggacggagag cccccctact
6901 tcaacgagcc acccctcaaa gccatgaaga tgattcggga caacctgcca ccccgactga
6961 agaacctgca caaggtgtcg ccatccctga agggcttcct ggaccgcctg ctggtgcgag
7021 accctgccca gcgggccacg gcagccgagc tgctgaagca cccattcctg gccaaggcag
7081 ggccgcctgc cagcatcgtg cccctcatgc gccagaaccg caccagatga tctagagggc
7141 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
7201 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
7261 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
7321 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
7381 tgggctctat ggactaaggt cacagcttgt ctgtcgatct gcgatctgca tctcaattag
7441 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
7501 gcccattctc cgccccatcg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
7561 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
7621 aaaaagtttc gacctgcagc ccagctagga atagctaggc caccatggcc atgcctttgt
7681 ctcaagaaga atccaccctc attgaaagag caacggctac aatcaacagc atccccatct
7741 ctgaggacta cagcgtcgcc agcgcagctc tctctagcga cggccgcatc ttcactggtg
7801 tcaatgtata tcattttact gggggacctt gtgcagaact cgtggtgctg ggcactgctg
7861 ctgctgcggc agctggcaac ctgacttgta tcgtcgcgat cggaaatgag aacaggggca
7921 tcttgagccc ctgcggacgg tgccgacagg tgcttctcga tctgcatcct gggatcaaag
7981 ccatagtgaa ggacagtgat ggacagccga cggcagttgg gattcgtgaa ttgctgccct
8041 ctggttatgt gtgggagggc taacagctta attcactcct caggtgcagt cgcgtcagac
8101 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
8161 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
8221 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
8281 gttttttaaa gcaagtaaaa cctctacaaa tgtgg
//
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