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pMS2-GFP载体 pMS2GFP质粒基本信息
pMS2-GFP质粒图谱 pMS2GFP载体图谱和pMS2-GFP载体序列 pMS2GFP质粒序列多克隆位点信息
pMS2-GFP质粒 pMS2GFP载体简介
pMS2-GFP质粒序列 pMS2GFP载体序列
LOCUS pMS2-GFP 6020 bp DNA circular SYN 12-JUL-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6020)
AUTHORS Biofeng
TITLE Direct Submission
JOURNAL Exported Jul 12, 2023 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6020
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS 750..776
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/note="This is a variant of the standard HA tag, which has
sequence YPYDVPDYA. Some anti-HA antibodies may not
recognize this variant."
/translation="YPYDVPDYA"
CDS 777..788
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
regulatory 1173..1182
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 1179..1895
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 1896..1916
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
intron 1975..2547
/label=beta-globin intron
/note="intron from rabbit beta-globin gene"
polyA_signal 2744..2799
/label=beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
promoter complement(3154..3172)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(3179..3195)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 3337..3792
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3818..3922
/gene="bla"
/label=AmpR promoter
CDS 4107..4486
/label=ISS
/note="immunostimulatory sequence from the AmpR gene;
contains unmethylated CpG dinucleotides in the context of
5'-AACGTT-3' (Sato et al., 1996)"
rep_origin 4955..5543
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 5831..5852
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 5867..5897
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5905..5921
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 5929..5945
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
promoter 5966..5984
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
1 tctagagtcg acctgcagcc caagcttggg ctgaacatcg agcgccaggg ctccgtaaag
61 ctactagagc acaggcggtg ccccaacgtc ctggggcctc tccactaata acggctactt
121 ccaattgatt ggacgcgcca tcttgcctgc cttatgcata ttcagcggtg aactgaatat
181 tcatgaacga ggcccgtccc gtccctccct cccttccccc ccccccggaa cccgctccgg
241 aggacccgaa gggccccgcc ttcattaccg atgcgtagga caaaccattt tcccgatgtg
301 tgtgggggga tactaatgag agactttagc tgaaaatgag cctgaactcc gaagctgagt
361 aaaaatggcc taactttatc ctccgttctg taagtcctcg gtttgagtgc acgggaaacc
421 cgaaaggagg acgacaggac caggaacatt ctcctcctcc tgtcgcgtca gaaagaacac
481 ccaaccaggg agccggagcc ctagcgtcaa caactccgcc gcgcgcgctc cgtgtaggcc
541 ggtgcgggcg gccccgtagc gcaagggagg gcgggaaagg aaggggcggg acacaagggc
601 gaatctataa agggcgtcac tcagccagtt ctctcctcag aagcgccgag agcgcgaccg
661 ggacggttgg agaagaaggt ggctcccgga agggggagag acaaactgcc gtaacctctg
721 ccgttcagga tcactagtcc accatgggct acccctacga cgtgcccgac tacgccatcg
781 aaggccgcca tatgctagcc gttaaaatgg cttctaactt tactcagttc gttctcgtcg
841 acaatggcgg aactggcgac gtgactgtcg ccccaagcaa cttcgctaac gggatcgctg
901 aatggatcag ctctaactcg cgttcacagg cttacaaagt aacctgtagc gttcgtcaga
961 gctctgcgca gaatcgcaaa tacaccatca aagtcgaggt gcctaaaggc gcctggcgtt
1021 cgtacttaaa tatggaacta accattccaa ttttcgccac gaattccgac tgcgagctta
1081 ttgttaaggc aatgcaaggt ctcctaaaag atggaaaccc gattccctca gcaatcgcag
1141 caaactccgg catctacgcg gatccaccgg tcgccaccat ggtgagcaag ggcgaggagc
1201 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
1261 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
1321 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
1381 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
1441 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
1501 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
1561 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
1621 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
1681 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
1741 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
1801 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
1861 ccgggatcac tctcggcatg gacgagctgt acaagccaaa aaagaagaga aaggtataaa
1921 tcgattctgc agtagctcgc cggcagctga ctagctagag ctgagaactt cagggtgagt
1981 ttggggaccc ttgattgttc tttctttttc gctattgtaa aattcatgtt atatggaggg
2041 ggcaaagttt tcagggtgtt gtttagaatg ggaagatgtc ccttgtatca ccatggaccc
2101 tcatgataat tttgtttctt tcactttcta ctctgttgac aaccattgtc tcctcttatt
2161 ttcttttcat tttctgtaac tttttcgtta aactttagct tgcatttgta acgaattttt
2221 aaattcactt ttgtttattt gtcagattgt aagtactttc tctaatcact tttttttcaa
2281 ggcaatcagg gtatattata ttgtacttca gcacagtttt agagaacaat tgttataatt
2341 aaatgataag gtagaatatt tctgcatata aattctggct ggcgtggaaa tattcttatt
2401 ggtagaaaca actacaccct ggtcatcatc ctgcctttct ctttatggtt acaatgatat
2461 acactgtttg agatgaggat aaaatactct gagtccaaac cgggcccctc tgctaaccat
2521 gttcatgcct tcttctcttt cctacagctc ctgggcaacg tgctggttgt tgtgctgtct
2581 catcattttg gcaaagaatt cactcctcag gtgcaggctg cctatcagaa ggtggtggct
2641 ggtgtggcca atgccctggc tcacaaatac cactgagatc tttttccctc tgccaaaaat
2701 tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt
2761 ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg
2821 gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat
2881 atgctggctg ccatgaacaa aggttggcta taaagaggtc atcagtatat gaaacagccc
2941 cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata
3001 ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta
3061 gccagatttt tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatgg
3121 agatccctcg agcggccgcg gtacccaatt cgccctatag tgagtcgtat tacaattcac
3181 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
3241 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
3301 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa
3361 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
3421 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
3481 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
3541 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
3601 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
3661 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
3721 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
3781 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
3841 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
3901 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
3961 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
4021 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
4081 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
4141 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
4201 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
4261 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
4321 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaaaca
4381 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
4441 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
4501 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
4561 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
4621 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
4681 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
4741 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
4801 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
4861 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
4921 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
4981 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
5041 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
5101 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
5161 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
5221 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
5281 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
5341 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
5401 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
5461 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
5521 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
5581 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
5641 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
5701 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
5761 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
5821 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
5881 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
5941 atgaccatga ttacgccaag ctcgaaatta accctcacta aagggaacaa aagctggagc
6001 tccaccgcgg tggcggccgc
//
