pIU424

LOCUS       pIU424          6400 bp DNA     circular SYN 15-JUL-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6400)
  AUTHORS   Westlake University
  TITLE     Direct Submission
  JOURNAL   Exported Sep 14, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6400
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     213..241
                     /label=OGT82-1-F
     misc_feature    232..819
                     /label=CMV
     primer_bind     863..882
                     /label=T7
     primer_bind     896..939
                     /label=OGT69-NLUC-F
     primer_bind     911..948
                     /label=OGT38-Nluc-F
     primer_bind     917..945
                     /label=OGT140-nluc-F
     primer_bind     920..943
                     /label=oSCL084
     primer_bind     921..946
                     /label=OGT71-NLUC-F
     misc_feature    922..990
                     /label=Secretory (IgK)
                     /label=nonstandard type:  signal
     primer_bind     complement(988..1008)
                     /label=OGT150-1-R
     primer_bind     complement(990..1014)
                     /label=OGT84-1-R
     primer_bind     1009..1033
                     /label=OGT150-3-F
     misc_feature    1012..1521
                     /label=NLuc (nano Luciferase)
                     /label=nonstandard type:  reporter
     primer_bind     1012..1036
                     /label=OGT84-3-F
     misc_feature    1042..1044
                     /label=cyt-STOP (TGG->TGA)
     primer_bind     complement(1505..1545)
                     /label=OGT69-NLUC-R
     primer_bind     complement(1505..1524)
                     /label=OGT140-nluc-R
     primer_bind     complement(1505..1521)
                     /label=OGT71-NLUC-R
     primer_bind     complement(1505..1521)
                     /label=OGT84-3-R
     primer_bind     complement(1511..1538)
                     /label=oSCL090
     primer_bind     complement(1511..1522)
                     /label=oSCL085
     primer_bind     1523..1563
                     /label=oIU424-1
     primer_bind     1542..1567
                     /label=oIU419-1
     misc_feature    1546..1959
                     /label=Coh2
     primer_bind     1546..1566
                     /label=oDJ125-1
     primer_bind     1546..1566
                     /label=oIU80-3
     primer_bind     1546..1563
                     /label=oSCL010
     primer_bind     complement(1938..1983)
                     /label=oIU424-2
     primer_bind     complement(1938..1959)
                     /label=oIU123-2
     primer_bind     complement(1938..1959)
                     /label=oIU419-2
     primer_bind     complement(1941..1959)
                     /label=oDJ125-2
     primer_bind     complement(1942..1960)
                     /label=oSCL011
     primer_bind     complement(1964..1989)
                     /label=oIU419-4
     terminator      1997..2224
                     /label=bGH PA
     misc_feature    2892..2969
                     /label=SV40 ORI
     CDS             3111..3902
                     /label=Kan/neoR
     primer_bind     4176..4200
                     /label=OGT140-nluc-R
     primer_bind     complement(4242..4262)
                     /label=M13-rev
     misc_binding    complement(4268..4290)
                     /label=LacO
     promoter        complement(4295..4324)
                     /label=lac
     CDS             complement(5407..6066)
                     /label=AmpR
     promoter        complement(6306..6334)
                     /label=Amp prom
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagctgccac catgactagt gagacagaca cactcctgct atgggtactg
      961 ctgctctggg ttccaggttc cactggtgac gctagtggtg gttctggtat ggtcttcaca
     1021 ctcgaagatt tcgttgggga ctggcgacag acagccggct acaacctgga ccaagtcctt
     1081 gaacagggag gtgtgtccag tttgtttcag aatctcgggg tgtccgtaac tccgatccaa
     1141 aggattgtcc tgagcggtga aaatgggctg aagatcgaca tccatgtcat catcccgtat
     1201 gaaggtctga gcggcgacca aatgggccag atcgaaaaaa tttttaaggt ggtgtaccct
     1261 gtggatgatc atcactttaa ggtgatcctg cactatggca cactggtaat cgacggggtt
     1321 acgccgaaca tgatcgacta tttcggacgg ccgtatgaag gcatcgccgt gttcgacggc
     1381 aaaaagatca ctgtaacagg gaccctgtgg aacggcaaca aaattatcga cgagcgcctg
     1441 atcaaccccg acggctccct gctgttccga gtaaccatca acggagtgac cggctggcgg
     1501 ctgtgcgaac gcattctggc gggaggcggc agcggagaat tcatggtggt ggtggagatc
     1561 ggcaaggtga ccggcagcgt gggaaccacc gtggagatcc ccgtgtactt cagaggcgtg
     1621 cccagcaagg gcatcgccaa ctgcgacttc gtgttcaggt acgaccccaa cgtgctggag
     1681 atcatcggca tcgaccccgg cgacatcatc gtggacccca accccaccaa aagcttcgac
     1741 accgccatct accccgacag gaagatcatc gtgttcctgt ttgccgagga cagcggcacc
     1801 ggagcctacg ccatcaccaa ggacggcgtg ttcgccaaga tcagggccac cgtgaagtcc
     1861 tccgcccctg gctacatcac cttcgacgag gtgggcggct tcgccgacaa cgacctggtg
     1921 gagcagaagg tgagcttcat cgacggcggc gtgaacgtgt agtctagagg gcccgtttaa
     1981 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     2041 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     2101 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     2161 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     2221 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
     2281 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     2341 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     2401 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     2461 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     2521 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     2581 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     2641 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
     2701 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     2761 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     2821 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     2881 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     2941 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
     3001 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
     3061 tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     3121 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     3181 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     3241 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
     3301 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     3361 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     3421 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     3481 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     3541 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     3601 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
     3661 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     3721 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     3781 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     3841 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     3901 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
     3961 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     4021 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
     4081 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     4141 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     4201 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     4261 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     4321 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     4381 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     4441 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     4501 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     4561 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     4621 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     4681 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     4741 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     4801 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     4861 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     4921 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     4981 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     5041 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     5101 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     5161 caaacaaacc accgctggta gcggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     5221 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     5281 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     5341 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     5401 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     5461 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     5521 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     5581 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     5641 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     5701 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     5761 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     5821 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     5881 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     5941 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     6001 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     6061 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     6121 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     6181 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     6241 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     6301 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     6361 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//