| 质粒名称: | pAAV-fCAMK-GFP |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 22917 |
| 存储实验室: | Edward Callaway |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 4000 bp |
| 完整质粒大小: | 8300 bp |
| 载体类型: | AAV,哺乳细胞表达 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | fugu α-calcium/calmodulin-dependent protein kinase II promoter driving GFP |
| 物种来源: | takifugu rubripes (河豚) |
| 插入大小: | 4300 bp |
| 克隆方法: | 限制性内切酶 |
| 5' 测序引物 | Unknown |
| 3' 测序引物 | SP6 |
|
文献引用方法 |
|
| 材料和方法部分: | pAAV-fCAMK-GFP was a gift from Edward Callaway (爱迪基因 plasmid # 22917) |
| 参考文献部分: | Short Promoters in Viral Vectors Drive Selective Expression in Mammalian Inhibitory Neurons, but do not Restrict Activity to Specific Inhibitory Cell-Types. Nathanson JL, Jappelli R, Scheeff ED, Manning G, Obata K, Brenner S, Callaway EM. Front Neural Circuits. 2009 . 3():19. 10.3389/neuro.04.019.2009 PubMed 19949461 |
买家导航
pAAV-fCAMK-GFP载体质粒基本信息
pAAV-fCAMK-GFP质粒图谱载体图谱和pAAV-fCAMK-GFP载体序列质粒序列多克隆位点信息
pAAV-fCAMK-GFP质粒载体简介
pAAV-fCAMK-GFP质粒序列载体序列
LOCUS pAAV-fCAMK-GFP 8291 bp ds-DNA circular SYN 10-JAN-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pAAV-fCAMK-GFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8291)
AUTHORS Nathanson JL, Jappelli R, Scheeff ED, Manning G, Obata K, Brenner S,
Callaway EM
TITLE Short Promoters in Viral Vectors Drive Selective Expression in
Mammalian Inhibitory Neurons, but do not Restrict Activity to
Specific Inhibitory Cell-Types.
JOURNAL Front Neural Circuits. 2009 . 3():19.
PUBMED 19949461
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|pAAV-fCAMK-GFP|
FEATURES Location/Qualifiers
source 1..8291
/organism="synthetic DNA construct"
/mol_type="other DNA"
repeat_region 7..136
/label=AAV2 ITR
intron 2840..3315
/label=-beta-globin intron
/note="internally truncated intron from human beta-globin"
primer_bind 3397..3413
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 3397..3413
/label=pBluescriptSK
/note="For pBluescript vector"
regulatory 3446..3455
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 3452..4168
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(3497..3518)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(3758..3777)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 4105..4126
/label=EGFP-C
/note="EGFP, forward primer"
promoter complement(4229..4247)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(4230..4247)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(4267..4284)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 4273..4384
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 5075..5588
/direction=RIGHT
/label=M13 ori
/note="M13 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5162..5181)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 5372..5393
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(6069..6088)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 6188..6210
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(6248..6266)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 6334..6438
/gene="bla"
/label=AmpR promoter
CDS 6439..7299
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(6657..6676)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7470..8058
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 7959..7978
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 8212..8229
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 cagcagctgc gcgctcgctc gctcactgag gccgcccggg caaagcccgg gcgtcgggcg
61 acctttggtc gcccggcctc agtgagcgag cgagcgcgca gagagggagt ggccaactcc
121 atcactaggg gttccttgta gttaatgatt aacccgccat gctacttatc tacgtagcca
181 tgctctagag gatccttcga aaagcttgta tacggccggc cacgcgtagt ctggagacca
241 agttcaggac tccggagacc cagtccaggc ctctggggtt ccagtccagg acaataaaga
301 agcagttcag gattctaaag aaccaatcca ggatgcaggc tctcagccat gatgtaattt
361 tgcaggaaat agattttctg ctctcttctc atcctacatg aactctgcaa aatacagaat
421 tggtttctta tcatcttcac tgtgaccagg gagtggaatc attgaggcat gaacacaggt
481 cttaattctg aatgtgtttg tgtcacagac tattagcttt actcagctca tactttcaca
541 gagattaatc atgaattatg aatcatgaaa tgatcttgat gaataaatct tcacactgga
601 acatcttcat ttccgtgctt ctgttgtggg tgaaagatca ctgctccctt ctggctggat
661 aaaaaaaaaa gacatcaaga aatatgaaga tatttaaatt ataaatggtg gtagtgacga
721 ctggtggtgc agttcaaatc accaggtaat caatcgccga tttgaaatat gctgtacccg
781 cccacagccg ccaggggacg ctagtcaaca acaggacatt taaatgtcag tgaaggaatt
841 tatcctgtaa aatcttgcga tgtgccaata ttaatgccaa aaaaaactga tataacactg
901 gctggagaat gaataatcat cagatgagtt tacataagcg ataatggtgt ttggtctgtg
961 tttgaaattg ctggttttgt tattctctgc aatattgctg aattgtgaac tgaaaggttt
1021 caaccagaca cataagctga atcgaatcga tgagttttaa tgaggaaacg attgcttgtg
1081 tcaaaagagg catctaatgg taaaattgtt aagggcagct ttaagcaatt gatggaaaca
1141 gctggtgctg agctcacgag gtcacatttg catctctatc taagtgatat tgcactaatg
1201 gaagaatcca tttaaaagtt gggatcccct ccatttaata gaccacctgc gtctgacctt
1261 ctgtcaccgt gtcatctgct acagtgggaa acgatcataa agcagcatgt gacatatgtt
1321 tcctcattag tccagtgctc ccagtgcccc cagtgctgcc ttcagtgagc cgtctgttta
1381 attccacttc tgtcaaacaa acatggagtt tgaactctta acgatggtgt tttgtttgag
1441 tccagcagtc atgcttcaag caaccgtgag caggtctttc taatatgaat tctccttacc
1501 agccaattcc tgctcctcct cctcttcctc ctcctcttct gctgggacag cgaggaggct
1561 ccaaagaggg cagccatgca gaactgatga gtgacagaaa gagaggagca gaattgagag
1621 aggctgctcc cggcagacac aaacaaaccc caaaatagac taaagaacaa gaaggctcag
1681 tccctctgac ctcaggttga aaagacacta cagacagctg caatatcacc ctcgctccgc
1741 tcaccggttc agttacacac acccgattta acagtttgtt ctttttcttc acatgcagca
1801 aaccaaagtc cgctcagatg gtgaatgggt tgtaaatttg ctattgtaac cacgaacaac
1861 agagcaggtg gggtaaatct agcaccagtt gtcaggcctt ctctgtgagt ctgttcattt
1921 cgtctgtcct aaaattcctt atgctggttt tctttattcc ttcaccattg gtgggagcgt
1981 cccagttccc cccccggttc cattactgca cgtagtatcg gaccattcag tgtccttgga
2041 tacgctggac gcagactcac acatgaaatt tcaggtaaac gccgcctgtt gtgtgttgtt
2101 cgcctcccac aggtcacctc ctctctggaa aatcttactg cgagactttg tagtactggt
2161 gagtagcaag agcagcttta tcttgtagta gcactgaagt tagcacaagt ataagcagtc
2221 agatcggaag taattttgaa tgtgatggga gctgaggagt ggcgttccgg acgtgttctc
2281 tatggttttt ctatgtacaa taatatttat agtactgcag ttttatttcc cgaataatct
2341 gatgctgttt gcagttactt tccagcacac acagagggga tgcagttacc atggtgaccc
2401 tgtgcatgtg agtgtacatg catgcgtgtg tgtgtctgtc ccggtctgtg attggctgat
2461 gggccagggt gggtgggcag gtggagcgtc tgaaagcatc tttctcccac agacccggac
2521 agagagacag agagacacag atctcctaac agctctctcc ttcttccctt catcttcctc
2581 cttttcctcc tcttcctcat cctcctcctc ctcttcctct cctcgggact gaaactatct
2641 ctccatcact cttctactct ctctattttc tctcctccat ccttctggca gacagaacgg
2701 cagctctaac tcttggcctc cttcgtctcc ggcatcatca tcttcagtgg aagacggaca
2761 gggaccccct tgaccctcga cctacagggt caccagggat gctagcggcg cgccgagctc
2821 ggatcctgag aacttcaggg tgagtctatg ggacccttga tgttttcttt ccccttcttt
2881 tctatggtta agttcatgtc ataggaaggg gagaagtaac agggtacaca tattgaccaa
2941 atcagggtaa ttttgcattt gtaattttaa aaaatgcttt cttcttttaa tatacttttt
3001 tgtttatctt atttctaata ctttccctaa tctctttctt tcagggcaat aatgatacaa
3061 tgtatcatgc ctctttgcac cattctaaag aataacagtg ataatttctg ggttaaggca
3121 atagcaatat ttctgcatat aaatatttct gcatataaat tgtaactgat gtaagaggtt
3181 tcatattgct aatagcagct acaatccagc taccattctg cttttatttt atggttggga
3241 taaggctgga ttattctgag tccaagctag gcccttttgc taatcatgtt catacctctt
3301 atcttcctcc cacagctcct gggcaacgtg ctggtctgtg tgctggccca tcactttggc
3361 aaagcacgtg aattcgaaga tctggccgcc tgggcctcta gaactagtgg atcccccggg
3421 ctgcaggaat tcgatgctac cggtcgccac catggtgagc aagggcgagg agctgttcac
3481 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
3541 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
3601 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
3661 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
3721 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
3781 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
3841 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
3901 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
3961 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
4021 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
4081 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
4141 cactctcggc atggacgagc tgtacaagtc cggactcaga tcctactagt aggatctcga
4201 gtttaaaccg tacgggaaac ggccctattc tatagtgtca cctaaatgct agagctcgct
4261 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
4321 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
4381 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
4441 agggggagga ttgggaagac aatagcgcgg ccgctctaga gcatggctac gtagataagt
4501 agcatggcgg gttaatcatt aactacaagg aacccctagt gatggagttg gccactccct
4561 ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct
4621 ttgcccgggc ggcctcagtg agcgagcgag cgcgccagct ggcgtaatag cgaagaggcc
4681 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggaa ttccagacga
4741 ttgagcgtca aaatgtaggt atttccatga gcgtttttcc tgttgcaatg gctggcggta
4801 atattgttct ggatattacc agcaaggccg atagtttgag ttcttctact caggcaagtg
4861 atgttattac taatcaaaga agtattgcga caacggttaa tttgcgtgat ggacagactc
4921 ttttactcgg tggcctcact gattataaaa acacttctca ggattctggc gtaccgttcc
4981 tgtctaaaat ccctttaatc ggcctcctgt ttagctcccg ctctgattct aacgaggaaa
5041 gcacgttata cgtgctcgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc
5101 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
5161 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
5221 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
5281 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
5341 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
5401 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
5461 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
5521 tttacaattt aaatatttgc ttatacaatc ttcctgtttt tggggctttt ctgattatca
5581 accggggtac atatgattga catgctagtt ttacgattac cgttcatcga ttctcttgtt
5641 tgctccagac tctcaggcaa tgacctgata gcctttgtag agacctctca aaaatagcta
5701 ccctctccgg catgaattta tcagctagaa cggttgaata tcatattgat ggtgatttga
5761 ctgtctccgg cctttctcac ccgtttgaat ctttacctac acattactca ggcattgcat
5821 ttaaaatata tgagggttct aaaaattttt atccttgcgt tgaaataaag gcttctcccg
5881 caaaagtatt acagggtcat aatgtttttg gtacaaccga tttagcttta tgctctgagg
5941 ctttattgct taattttgct aattctttgc cttgcctgta tgatttattg gatgttggaa
6001 ttcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc
6061 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
6121 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
6181 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
6241 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
6301 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
6361 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
6421 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
6481 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
6541 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
6601 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
6661 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
6721 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
6781 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
6841 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
6901 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
6961 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
7021 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
7081 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
7141 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
7201 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
7261 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
7321 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
7381 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
7441 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
7501 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
7561 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
7621 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
7681 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
7741 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
7801 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
7861 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
7921 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
7981 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
8041 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
8101 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
8161 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
8221 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
8281 attcattaat g
//
