| 质粒名称: | pAAVLINK-EF1alpha-HA-5'-BRD4 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001973 |
| 产品用途: | 5' AAVLINK plasmid for BRD4 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1alpha |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | BRD4 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1alpha-HA-5'-BRD4
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1alpha-HA-5'-BRD4载体质粒基本信息
pAAVLINK-EF1alpha-HA-5'-BRD4质粒图谱载体图谱和pAAVLINK-EF1alpha-HA-5'-BRD4载体序列质粒序列多克隆位点信息
pAAVLINK-EF1alpha-HA-5'-BRD4质粒载体简介
pAAVLINK-EF1alpha-HA-5'-BRD4质粒序列载体序列
LOCUS pAAVLINK-EF1alpha-HA-5'-BRD4 5693 bp DNA circular SYN 10-AUG-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5693)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 19, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5693
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
/note="color: #ffe4c4"
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..2833
/codon_start=1
/gene="BRD4"
/gene_synonym="CAP; FSHRG4; HUNK1; HUNKI; MCAP"
/product="bromodomain-containing protein 4 isoform long"
/EC_number="3.1.1.31"
/label=BRD4
/note="isoform long is encoded by transcript variant 4;
chromosome-associated protein; bromodomain-containing
protein 4; mitotic chromosome-associated protein; female
sterile homeotic related gene 4"
/db_xref="GI:1825629150"
/db_xref="CCDS:CCDS12328.1"
/db_xref="GeneID:23476"
/db_xref="HGNC:HGNC:13575"
/db_xref="MIM:608749"
/protein_id="NP_001366220.1"
/translation="SAESGPGTRLRNLPVMGDGLETSQMSTTQAQAQPQPANAASTNPP
PPETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPM
DMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINEL
PTEETEIMIVQAKGRGRGRKETGTAKPGVSTVPNTTQASTPPQTQTPQPNPPPVQATPH
PFPAVTPDLIVQTPVMTVVPPQPLQTPPPVPPQPQPPPAPAPQPVQSHPPIIAATPQPV
KTKKGVKRKADTTTPTTIDPIHEPPSLPPEPKTTKLGQRRESSRPVKPPKKDVPDSQQH
PAPEKSSKVSEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDM
STIKSKLEAREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPD
EPEEPVVAVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQL
KAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKRKEEVEENKKSKAKEPPPKKTKKNN
SSNSNVSKKEPAPMKSKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHI
IQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKM
K"
misc_feature 1388..1393
/label=SDS
misc_feature 1391..1396
/label=SDS
misc_feature 2045..2050
/label=SDS
misc_feature 2256..2261
/label=SDS
misc_feature 2447..2452
/label=SDS
misc_feature 2450..2455
/label=SDS
misc_feature 2760..2765
/label=SDS
intron 2834..2874
/label=Splicing Donor
misc_feature 2878..2911
/label=lox2272-JT1571
repeat_region 2956..3096
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 2956..3085
/label=AAV2 ITR
repeat_region 2957..3097
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 3171..3626
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3908..4012
/gene="bla"
/label=AmpR promoter
CDS 4013..4873
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5044..5632
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatcttc tgcggagagc ggccctggga cgagattgag
781 aaatctgcca gtaatggggg atggactaga aacttcccaa atgtctacaa cacaggccca
841 ggcccaaccc cagccagcca acgcagccag caccaacccc ccgcccccag agacctccaa
901 ccctaacaag cccaagaggc agaccaacca actgcaatac ctgctcagag tggtgctcaa
961 gacactatgg aaacaccagt ttgcatggcc tttccagcag cctgtggatg ccgtcaagct
1021 gaacctccct gattactata agatcattaa aacgcctatg gatatgggaa caataaagaa
1081 gcgcttggaa aacaactatt actggaatgc tcaggaatgt atccaggact tcaacactat
1141 gtttacaaat tgttacatct acaacaagcc tggagatgac atagtcttaa tggcagaagc
1201 tctggaaaag ctcttcttgc aaaaaataaa tgagctaccc acagaagaaa ccgagatcat
1261 gatagtccag gcaaaaggaa gaggacgtgg gaggaaagaa acagggacag caaaacctgg
1321 cgtttccacg gtaccaaaca caactcaagc atcgactcct ccgcagaccc agacccctca
1381 gccgaatcct cctcctgtgc aggccacgcc tcaccccttc cctgccgtca ccccggacct
1441 catcgtccag acccctgtca tgacagtggt gcctccccag ccactgcaga cgcccccgcc
1501 agtgcccccc cagccacaac ccccacccgc tccagctccc cagcccgtac agagccaccc
1561 acccatcatc gcggccaccc cacagcctgt gaagacaaag aagggagtga agaggaaagc
1621 agacaccacc acccccacca ccattgaccc cattcacgag ccaccctcgc tgcccccgga
1681 gcccaagacc accaagctgg gccagcggcg ggagagcagc cggcctgtga aacctccaaa
1741 gaaggacgtg cccgactctc agcagcaccc agcaccagag aagagcagca aggtctcgga
1801 gcagctcaag tgctgcagcg gcatcctcaa ggagatgttt gccaagaagc acgccgccta
1861 cgcctggccc ttctacaagc ctgtggacgt ggaggcactg ggcctacacg actactgtga
1921 catcatcaag caccccatgg acatgagcac aatcaagtct aaactggagg cccgtgagta
1981 ccgtgatgct caggagtttg gtgctgacgt ccgattgatg ttctccaact gctataagta
2041 caaccctcct gaccatgagg tggtggccat ggcccgcaag ctccaggatg tgttcgaaat
2101 gcgctttgcc aagatgccgg acgagcctga ggagccagtg gtggccgtgt cctccccggc
2161 agtgccccct cccaccaagg ttgtggcccc gccctcatcc agcgacagca gcagcgatag
2221 ctcctcggac agtgacagtt cgactgatga ctctgaggag gagcgagccc agcggctggc
2281 tgagctccag gagcagctca aagccgtgca cgagcagctt gcagccctct ctcagcccca
2341 gcagaacaaa ccaaagaaaa aggagaaaga caagaaggaa aagaaaaaag aaaagcacaa
2401 aaggaaagag gaagtggaag agaataaaaa aagcaaagcc aaggaacctc ctcctaaaaa
2461 gacgaagaaa aataatagca gcaacagcaa tgtgagcaag aaggagccag cgcccatgaa
2521 gagcaagccc cctcccacgt atgagtcgga ggaagaggac aagtgcaagc ctatgtccta
2581 tgaggagaag cggcagctca gcttggacat caacaagctc cccggcgaga agctgggccg
2641 cgtggtgcac atcatccagt cacgggagcc ctccctgaag aattccaacc ccgacgagat
2701 tgaaatcgac tttgagaccc tgaagccgtc cacactgcgt gagctggagc gctatgtcac
2761 ctcctgtttg cggaagaaaa ggaaacctca agctgagaaa gttgatgtga ttgccggctc
2821 ctccaagatg aaggtaagta tcaaggttac aagacaggtt taaggagacc aataagctat
2881 tattcgtata ggatacttta tacgaagtta ttaagttatg gagaccggtg tggcacgtgc
2941 ggaccgagcg gccgcaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc
3001 gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg
3061 cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc
3121 ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg
3181 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
3241 cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
3301 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
3361 gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg
3421 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
3481 ccaaactgga acaacactca actctatctc gggctattct tttgatttat aagggatttt
3541 gccgatttcg gtctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
3601 taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc
3661 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
3721 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
3781 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
3841 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
3901 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
3961 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
4021 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
4081 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
4141 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
4201 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
4261 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
4321 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
4381 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
4441 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
4501 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
4561 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
4621 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
4681 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
4741 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
4801 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
4861 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
4921 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
4981 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
5041 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
5101 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
5161 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
5221 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
5281 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
5341 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
5401 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
5461 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
5521 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
5581 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
5641 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgt
//
