| 质粒名称: | pAAVLINK-EF1a-HA-EP400-A |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001747 |
| 产品用途: | 5' AAVLINK plasmid for EP400 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | EP400 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-HA-EP400-A
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-HA-EP400-A载体质粒基本信息
pAAVLINK-EF1a-HA-EP400-A质粒图谱载体图谱和pAAVLINK-EF1a-HA-EP400-A载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-EP400-A质粒载体简介
pAAVLINK-EF1a-HA-EP400-A质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-EP400-A 6900 bp DNA circular SYN 21-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6900)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 5, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6900
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 146..688
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 175..386
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 399..667
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 493..498
/label=SDS
CDS 704..730
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 737..4040
/codon_start=1
/label=EP400
/translation="MHHGTGPQNVQHQLQRSRACPGSEGEEQPAHPNPPPSPAAPFAPS
ASPSAPQSPSYQIQQLMNRSPATGQNVNITLQSVGPVVGGNQQITLAPLPLPSPTSPGF
QFSAQPRRFEHGSPSYIQVTSPLSQQVQTQSPTQPSPGPGQALQNVRAGAPGPGLGLCS
SSPTGGFVDASVLVRQISLSPSSGGHFVFQDGSGLTQIAQGAQVQLQHPGTPITVRERR
PSQPHTQSGGTIHHLGPQSPAAAGGAGLQPLASPSHITTANLPPQISSIIQGQLVQQQQ
VLQGPPLPRPLGFERTPGVLLPGAGGAAGFGMTSPPPPTSPSRTAVPPGLSSLPLTSVG
NTGMKKVPKKLEEIPPASPEMAQMRKQCLDYHYQEMQALKEVFKEYLIELFFLQHFQGN
MMDFLAFKKKHYAPLQAYLRQNDLDIEEEEEEEEEEEEKSEVINDEQQALAGSLVAGAG
STVETDLFKRQQAMPSTGMAEQSKRPRLEVGHQGVVFQHPGADAGVPLQQLMPTAQGGM
PPTPQAAQLAGQRQSQQQYDPSTGPPVQNAASLHTPLPQLPGRLPPAGVPTAALSSALQ
FAQQPQVVEAQTQLQIPVKTQQPNVPIPAPPSSQLPIPPSQPAQLALHVPTPGKVQVQA
SQLSSLPQMVASTRLPVDPAPPCPRPLPTSSTSSLAPVSGSGPGPSPARSSPVNRPSSA
TNKALSPVTSRTPGVVASAPTKPQSPAQNATSSQDSSQDTLTEQITLENQVHQRIAELR
KAGLWSQRRLPKLQEAPRPKSHWDYLLEEMQWMATDFAQERRWKVAAAKKLVRTVVRHH
EEKQLREERGKKEEQSRLRRIAASTAREIECFWSNIEQVVEIKLRVELEEKRKKALNLQ
KVSRRGKELRPKGFDALQESSLDSGMSGRKRKASISLTDDEVDDEEETIEEEEANEGVV
DHQTELSNLAKEAELPLLDLMKLYEGAFLPSSQWPRPKPDGEDTSGEEDADDCPGDRES
RKDLVLIDSLFIMDQFKAAERMNIGKPNAKDIADVTAVAEAILPKGSARVTTSVKFNAP
SLLYGALRDYQKIGLDWLAKLYRKNLNGILADEAGLGKTVQIIAFFAHLACNE"
misc_feature 1875..1880
/label=SDS
misc_feature 2019..2024
/label=SDS
misc_feature 2022..2027
/label=SDS
misc_feature 2025..2030
/label=SDS
misc_feature 2028..2033
/label=SDS
misc_feature 2037..2042
/label=SDS
misc_feature 2278..2283
/label=SDS
misc_feature 3036..3041
/label=SDS
misc_feature 3116..3121
/label=SDS
misc_feature 3501..3506
/label=SDS
misc_feature 3574..3579
/label=SDS
intron 4041..4081
/label=Splicing Donor
misc_feature 4085..4118
/label=loxP-JT1571
repeat_region 4163..4303
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4163..4292
/label=AAV2 ITR
rep_origin 4378..4833
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5115..5219
/gene="bla"
/label=AmpR promoter
CDS 5220..6080
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6251..6839
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtggatct gcgatcgctc cggtgcccgt cagtgggcag
181 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
361 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
421 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
481 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
541 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
601 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
661 acagatccaa gctgtgaccg gcgcctaccg tacggccacc atgtaccctt atgacgtacc
721 tgactatgct ggatctatgc accatggcac tggcccccag aacgtccagc atcagctgca
781 gaggtccagg gcctgccctg gcagcgaggg tgaggagcag ccggcccacc ccaacccacc
841 cccgtccccc gcagctccct tcgctccctc agcaagcccg tcggcacccc agtctcccag
901 ttatcaaata cagcagctga tgaataggag ccctgcaacc gggcagaacg tgaacatcac
961 cctgcagagc gtgggccctg tcgtcggggg aaaccagcag atcacactgg ccccactgcc
1021 gctccccagc cccacctctc caggcttcca gttcagcgct cagcctcggc ggtttgagca
1081 tgggtctcca tcatacattc aggtcacgtc ccccttgtcc cagcaggtcc agacccagag
1141 tcccacgcag cccagtccgg ggccggggca ggccttgcag aatgtgcgtg caggtgcccc
1201 tggccctggg ctgggcctct gcagcagcag ccctacaggg ggcttcgtgg atgccagcgt
1261 gctggtgagg cagatcagct tgagcccctc cagtggtgga cactttgtgt ttcaggatgg
1321 gtcagggctc acccagatcg cccagggagc ccaggttcag ctccagcacc cgggtacgcc
1381 catcacagtc cgagagcgga gaccctccca gccccacaca cagtcagggg gcaccatcca
1441 ccacctggga ccccagagcc ctgcagccgc gggtggggcc ggcctgcagc ccctggccag
1501 cccaagccac atcaccacgg ctaacttgcc accgcagatc agcagcatca tccagggcca
1561 gctggttcag cagcagcagg tgctgcaggg gccgccgctg ccccggcccc tgggcttcga
1621 gaggacaccc ggcgtgctgc tccccggggc tgggggcgca gcggggtttg ggatgacgtc
1681 cccacccccg cccaccagcc cttccaggac tgccgtgccc ccaggccttt ccagcctccc
1741 actcacgtct gtggggaaca cgggaatgaa gaaggttccc aagaagttag aggagattcc
1801 cccagcctct ccggagatgg cacagatgag gaagcagtgc ctggactatc attaccagga
1861 gatgcaggct ctgaaggagg tcttcaagga gtatttgatt gaactgtttt tcttgcaaca
1921 ctttcaaggg aacatgatgg atttcttagc tttcaagaag aaacattatg ccccattaca
1981 agcatatctt aggcagaatg atttggacat tgaagaagag gaggaggagg aggaagagga
2041 ggaagaaaaa tctgaggtta tcaatgacga gcagcaagcc ctcgcaggga gcctggtagc
2101 aggggccgga agcacagtag agacggacct gtttaagagg cagcaggcga tgccctccac
2161 aggtatggca gagcagtcta agaggcctcg ccttgaagtg ggtcaccaag gggtagtttt
2221 ccagcaccca ggggcggacg caggcgttcc tctccagcaa ctaatgccga ccgcacaagg
2281 aggaatgccc cccacgccgc aggccgcgca gctcgctgga cagaggcaga gtcagcagca
2341 gtatgacccc tccacggggc ctcccgtgca gaacgctgcc agcttgcaca ccccactgcc
2401 gcagctgccc gggaggctgc ccccagccgg tgttcccact gcagccctct cctctgcgct
2461 gcagtttgca cagcagccgc aagtggtaga ggcccagaca cagctccaaa tcccggtgaa
2521 gactcagcag cccaatgttc ccatccctgc accgcccagc agccaactcc ccatccctcc
2581 ctcgcagcct gcacagctgg ccctccacgt tcccacacct ggaaaggtgc aggtgcaggc
2641 ctctcagctt tcctccctgc cacagatggt agcatcgaca aggctccctg tggaccctgc
2701 cccgccctgc ccacggcctc tgcccacctc ttctacctcg tccctcgcgc ctgtgagtgg
2761 ctccggccca ggaccctccc ctgctcgatc ctctccagta aatagacctt cctcagccac
2821 caataaggca ctatctccag tcacttcccg gaccccaggg gtggtggcat ctgcccccac
2881 caaaccacag agtcctgctc agaatgccac ctcgtcccaa gacagttctc aggatacgct
2941 gacagaacaa ataactctgg agaaccaggt gcatcagcgc attgcggagc tgaggaaagc
3001 aggtctgtgg tcccagaggc gtctgccaaa gctgcaggag gccccacgcc ccaagtccca
3061 ctgggactat ctgctggagg agatgcagtg gatggccaca gactttgccc aggagaggag
3121 gtggaaggtg gctgctgcga agaagctcgt tagaactgtg gtgcgccatc acgaggagaa
3181 gcagctccgt gaagaaaggg ggaagaagga agagcagagc agactgaggc ggatagccgc
3241 ctccacggcc cgggagatag agtgcttttg gtcgaatatt gaacaggttg tggaaataaa
3301 actacgagta gaattagaag aaaaaaggaa gaaggcctta aatttacaga aagtttccag
3361 gagagggaaa gaattgagac ctaaaggatt tgacgcatta caggaaagtt ctctggattc
3421 aggaatgtct ggaagaaaaa gaaaagctag catatctttg actgatgacg aagtggacga
3481 tgaagaggaa acaattgaag aggaggaagc aaatgaaggc gttgtggacc accaaacaga
3541 actttctaat ttagccaagg aagctgagct gcccctcctg gacctgatga agctgtacga
3601 aggcgccttc ctgccgagtt ctcagtggcc ccggccgaag cctgatgggg aggacacaag
3661 cggagaggaa gatgcagatg actgtccagg cgacagggag agtcgcaagg acttggttct
3721 catcgactcg cttttcatca tggatcagtt caaagctgcc gagaggatga atatcgggaa
3781 gccaaacgcc aaggacattg cggacgtcac tgcggtggct gaagccatcc tgccgaaggg
3841 cagtgctcgg gtcacaacct cggtcaagtt taatgctcca tctttgttgt atggggctct
3901 cagagattat cagaagattg gcctggactg gctggccaaa ctttacagga agaatctcaa
3961 tggcatattg gcagatgaag ctgggctggg taaaacagtg cagatcattg ctttttttgc
4021 ccacctagct tgtaacgaag gtaagtatca aggttacaag acaggtttaa ggagaccaat
4081 aagctattat tcgtatagca tacattatac gaagttatta agttatggag accggtgtgg
4141 cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg
4201 cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc
4261 cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat
4321 tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg
4381 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
4441 cacttgccag cgccttagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
4501 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
4561 ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat
4621 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
4681 tcttgttcca aactggaaca acactcaact ctatctcggg ctattctttt gatttataag
4741 ggattttgcc gatttcggtc tattggttaa aaaatgagct gatttaacaa aaatttaacg
4801 cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct
4861 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
4921 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
4981 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
5041 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
5101 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
5161 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
5221 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
5281 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
5341 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
5401 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
5461 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
5521 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
5581 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
5641 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
5701 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
5761 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
5821 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
5881 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
5941 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
6001 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
6061 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
6121 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
6181 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
6241 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
6301 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
6361 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
6421 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
6481 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
6541 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
6601 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
6661 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
6721 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
6781 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
6841 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
//
