| 质粒名称: | pSL1292 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002430 |
| 产品用途: | Mammalian PhCMV-driven constitutive expression vector for MCP-specific SEAP-mRNA containing 8 tandem MS2-box repeats in the 5’-UTR (PhCMV-(MS2)8-SEAP-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | PhCMV |
| 拷贝数: | 未拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | SEAP |
| 物种来源: | synthetic |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Rationally designed light-inducible RNA-releasing protein for translational regulation and optogenetic control of gene therapies
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NA
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pSL1292
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pSL1292载体质粒基本信息
pSL1292质粒图谱载体图谱和pSL1292载体序列质粒序列多克隆位点信息
pSL1292质粒载体简介
pSL1292质粒序列载体序列
LOCUS pSL1292 7363 bp DNA circular SYN 02-APR-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7363)
AUTHORS Trial User
TITLE Direct Submission
JOURNAL Exported Aug 28, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7363
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 232..819
/label=CMV
primer_bind 863..882
/label=T7
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 880..942
/label=oSCL2270
misc_feature 895
/label=NheI
misc_feature 924..936
/label=linker
misc_feature 942..960
/label=MS2 box
misc_feature 966..975
/label=linker
misc_feature 981..999
/label=MS2 box
misc_feature 1005..1014
/label=linker
misc_feature 1005..1014
/label=linker
misc_feature 1013..1043
/label=hairpin
misc_feature 1019..1037
/label=MS2 box
misc_feature 1042..1054
/label=linker
misc_feature 1053..1083
/label=hairpin
misc_feature 1059..1077
/label=MS2 box
primer_bind complement(1076..1093)
/label=oSCL2271
primer_bind 1085..1111
/label=oSCL2270
misc_feature 1087..1088
/label=linker
misc_feature 1093..1105
/label=linker
misc_feature 1111..1129
/label=MS2 box
misc_feature 1135..1144
/label=linker
misc_feature 1150..1168
/label=MS2 box
misc_feature 1174..1183
/label=linker
misc_feature 1174..1183
/label=linker
misc_feature 1182..1212
/label=hairpin
misc_feature 1188..1206
/label=MS2 box
misc_feature 1211..1223
/label=linker
misc_feature 1222..1252
/label=hairpin
misc_feature 1228..1246
/label=MS2 box
primer_bind complement(1245..1274)
/label=oSCL2271
misc_feature 1256..1261
/label=linker
primer_bind 1269..1290
/label=Oscl31-3
misc_feature 1270..1273
/label=New Feature
misc_feature 1270..1273
/label=New Feature
misc_feature 1273
/label=EGFP
/note="note=""Fragment extracted from OWS233-Kpn1-EGFP-Xba1
[758 nt] : (KpnI[4] / XbaI[766])"
primer_bind 1276..1290
/label=oSCL775
primer_bind 1278..1290
/label=oSCL456
misc_feature 1284..2843
/label=SEAP
primer_bind complement(1472..1508)
/label=oSCL545
primer_bind 1489..1517
/label=oSCL544
primer_bind complement(2305..2336)
/label=oSCL546
primer_bind 2317..2349
/label=oSCL547
primer_bind 2402..2432
/label=Oscl50-1
primer_bind complement(2402..2432)
/label=Oscl50-2
primer_bind complement(2791..2843)
/label=oSCL795
primer_bind complement(2825..2840)
/label=oSCL475
primer_bind complement(2827..2883)
/label=oSCL548
primer_bind complement(2827..2869)
/label=oSCL083
primer_bind complement(2827..2843)
/label=Oscl31-4
primer_bind complement(2827..2843)
/label=oSCL457
primer_bind complement(2827..2843)
/label=oSCL776
polyA_signal 2835..2843
/label=SV40 late polyA
misc_feature 2887..2892
/label=EcoRI
misc_feature 2926..2931
/label=New Feature
misc_feature 2926..2931
/label=New Feature
primer_bind complement(2927..2946)
/label=oSCL2271
terminator 2960..3187
/label=bGH PA
polyA_signal 2963..3187
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 3233..3661
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3675..4004
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3855..3990
/label=SV40 ori
/note="SV40 origin of replication"
misc_feature 3855..3932
/label=SV40 ORI
CDS 4071..4865
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 5039..5160
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5205..5225)
/label=M13-rev
primer_bind complement(5209..5225)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
misc_binding complement(5231..5253)
/label=LacO
protein_bind 5233..5249
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5257..5287)
/label=lac promoter
/note="promoter for the E. coli lac operon"
promoter complement(5258..5287)
/label=lac
protein_bind 5302..5323
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(5611..6196)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(6367..7227)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(7228..7332)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 accggtggat ccggtctcaa actgcaacaa acaaatcacc accgtgagga tcacccacgg
961 tggtgaaagc cttagcctag ccgtgaggat cacccacggt aggcacaaga aattctaccc
1021 gtgaggatca cccacgggta gaatcacaaa gactacagcc gtgaggatca cccacggctg
1081 tagactcaaa ctgcaacaaa caaatcacca ccgtgaggat cacccacggt ggtgaaagcc
1141 ttagcctagc cgtgaggatc acccacggta ggcacaagaa attctacccg tgaggatcac
1201 ccacgggtag aatcacaaag actacagccg tgaggatcac ccacggctgt agactcaaac
1261 tggagacgaa gcttagcgcc accatgctgc tgctgctgct gctgctgggc ctgaggctac
1321 agctctccct gggcatcatc ccagttgagg aggagaaccc ggacttctgg aaccgcgagg
1381 cagccgaggc cctgggtgcc gccaagaagc tgcagcctgc acagacagcc gccaagaacc
1441 tcatcatctt cctgggcgat gggatggggg tgtctacggt gacagctgcc agaatcctaa
1501 aagggcagaa gaaggacaaa ctggggcctg agatacccct ggccatggac cgcttcccat
1561 atgtggctct gtccaagaca tacaatgtag acaaacatgt gccagacagt ggagccacag
1621 ccacggccta cctgtgcggg gtcaagggca acttccagac cattggcttg agtgcagccg
1681 cccgctttaa ccagtgcaac acgacacgcg gcaacgaggt catctccgtg atgaatcggg
1741 ccaagaaagc agggaagtca gtgggagtgg taaccaccac acgagtgcag cacgcctcgc
1801 cagccggcac ctacgcccac acggtgaacc gcaactggta ctcggacgcc gacgtgcctg
1861 cctcggcccg ccaggagggg tgccaggaca tcgctacgca gctcatctcc aacatggaca
1921 ttgacgtgat cctaggtgga ggccgaaagt acatgtttcg catgggaacc ccagaccctg
1981 agtacccaga tgactacagc caaggtggga ccaggctgga cgggaagaat ctggtgcagg
2041 aatggctggc gaagcgccag ggtgcccggt atgtgtggaa ccgcactgag ctcatgcagg
2101 cttccctgga cccgtctgtg acccatctca tgggtctctt tgagcctgga gacatgaaat
2161 acgagatcca ccgagactcc acactggacc cctccctgat ggagatgaca gaggctgccc
2221 tgcgcctgct gagcaggaac ccccgcggct tcttcctctt cgtggagggt ggtcgcatcg
2281 accatggtca tcatgaaagc agggcttacc gggcactgac tgagaccatc atgttcgacg
2341 acgccattga gagggcgggc cagctcacca gcgaggagga cacgctgagc ctcgtcactg
2401 ccgaccactc ccacgtgttc tccttcggag gctaccccct gcgagggagc tccatcttcg
2461 ggctggcccc tggcaaggcc cgggacagga aggcctacac ggtcctccta tacggaaacg
2521 gtccaggcta tgtgctcaag gacggcgccc ggccggatgt taccgagagc gagagcggga
2581 gccccgagta tcggcagcag tcagcagtgc ccctggacga agagacccac gcaggcgagg
2641 acgtggcggt gttcgcgcgc ggcccgcagg cgcacctggt tcacggcgtg caggagcaga
2701 ccttcatagc gcacgtcatg gccttcgccg cctgcctgga gccctacacc gcctgcgacc
2761 tggcgccccc cgccggcacc accgacgccg cgcacccggg ttacagcaga gtcggggcgg
2821 ccggccgctt cgagcagaca tgagcggccg ctcaatctcg aggggatcca ctagtccagt
2881 gtggtggaat tctgcagata tccagcacag tggcggccgc tcgagtctag agggcccgtt
2941 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
3001 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
3061 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
3121 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
3181 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
3241 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
3301 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
3361 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
3421 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
3481 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
3541 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
3601 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
3661 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
3721 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
3781 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
3841 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
3901 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
3961 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
4021 gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac
4081 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
4141 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
4201 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
4261 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
4321 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
4381 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
4441 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
4501 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
4561 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
4621 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
4681 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
4741 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
4801 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
4861 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
4921 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
4981 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
5041 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
5101 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
5161 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
5221 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
5281 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
5341 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
5401 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
5461 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
5521 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
5581 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
5641 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
5701 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
5761 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
5821 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
5881 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
5941 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
6001 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
6061 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
6121 cggcaaacaa accaccgctg gtagcggttt ttttgtttgc aagcagcaga ttacgcgcag
6181 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
6241 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
6301 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
6361 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
6421 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
6481 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
6541 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
6601 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
6661 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
6721 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
6781 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
6841 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
6901 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
6961 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
7021 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
7081 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
7141 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
7201 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
7261 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
7321 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtc
//
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