| 质粒名称: | Lenti-Cre |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 198712 |
| 存储实验室: | Tyler Jacks |
|
载体骨架基本信息 |
|
| 载体名称: | Lenti-LucOS |
| 载体出品公司: | Clontech |
| 空载体大小: | 5818bp |
| 完整质粒大小: | 7406bp |
| 载体类型: | 哺乳细胞表达, Lentiviral, Cre/Lox |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Pgk-Cre、Pgk promoter、Cre recombinase |
| 物种来源: | M. musculus (mouse); bacteriophage P1 |
| 插入大小: | 1588bp |
| 启动子: | PGK |
| 克隆方法: | Gibson Cloning |
| 3’克隆位点: | NotI (未破坏) |
| 5’测序引物 | GATCAGTGTGAGGGAGTGTAAAGCTGGTTT |
| 3’测序引物 | AGAGTAATTCAACCCCAAACAACAACGTTT |
|
文献引用方法 |
|
| 材料和方法部分: | Lenti-Cre was a gift from Tyler Jacks (Addgene plasmid # 198712 ; http://n2t.net/addgene:198712 ; RRID:Addgene_198712) |
| 参考文献部分: | Lymphocyte networks are dynamic cellular communities in the immunoregulatory landscape of lung adenocarcinoma. Gaglia G, Burger ML, Ritch CC, Rammos D, Dai Y, Crossland GE, Tavana SZ, Warchol S, Jaeger AM, Naranjo S, Coy S, Nirmal AJ, Krueger R, Lin JR, Pfister H, Sorger PK, Jacks T, Santagata S. Cancer Cell. 2023 May 8;41(5):871-886.e10. doi: 10.1016/j.ccell.2023. |
买家导航
Lenti-Cre载体质粒基本信息
Lenti-Cre质粒图谱载体图谱和Lenti-Cre载体序列质粒序列多克隆位点信息
Lenti-Cre质粒载体简介
Lenti-Cre质粒序列载体序列
LOCUS Lenti-Cre 7393 bp DNA circular SYN 15-DEC-2025
DEFINITION For lentiviral expression of Cre recombinase in mammalian cells..
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7393)
AUTHORS Gaglia G, Burger ML, Ritch CC, Rammos D, Dai Y, Crossland GE, Tavana
SZ, Warchol S, Jaeger AM, Naranjo S, Coy S, Nirmal AJ, Krueger R,
Lin JR, Pfister H, Sorger PK, Jacks T, Santagata S
TITLE Lymphocyte networks are dynamic cellular communities in the
immunoregulatory landscape of lung adenocarcinoma.
JOURNAL Cancer Cell. 2023 May 8;41(5):871-886.e10. doi:
10.1016/j.ccell.2023.03.015. Epub 2023 Apr 13.
PUBMED 37059105
REFERENCE 2 (bases 1 to 7393)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Dec 15, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1016/j.ccell.2023.03.015"; journalName: "Cancer Cell"; date:
"2023-05-8- 8"; volume: "41"; issue: "5"; pages: "871-886.e10"
COMMENT Sequence Label: Lenti-Cre
FEATURES Location/Qualifiers
source 1..7393
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind complement(46..65)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 237..616
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 618..816
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 771..791
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 834..1014
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 1061..1186
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1683..1916
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 2101..2145
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2443..2560
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2661..3148
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(2696..2719)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind 3081..3100
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
CDS 3179..3199
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 3200..4228
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL
SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
GD"
misc_feature 4281..4869
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(4334..4354)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(4752..4763)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(4872..4888)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4873..4889)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 5398..5578
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
rep_origin complement(5640..6228)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5720..5739)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(6399..7190,7191..7259))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7022..7041
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7260..7364)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
61 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
121 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
181 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
241 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
301 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
361 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
421 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
481 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
541 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
601 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
661 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
721 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
781 cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc
841 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
901 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
961 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
1021 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
1081 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
1141 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
1201 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
1261 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
1321 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
1381 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
1441 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
1501 aagtaagacc accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat
1561 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
1621 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
1681 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
1741 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
1801 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
1861 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
1921 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
1981 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
2041 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
2101 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
2161 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
2221 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
2281 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
2341 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
2401 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
2461 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
2521 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
2581 cagagatcca gtttggttag taccgggccc gggtcgacta gtgatcagtg tgagggagtg
2641 taaagctggt ttattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg
2701 cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca
2761 ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc
2821 ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac
2881 gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca
2941 atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg
3001 ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggg cgggcgcccg
3061 aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt
3121 ctcctcttcc tcatctccgg gagacccgac attagcgcta cagcttaagc ggaccatgcc
3181 caagaagaag aggaaggtgt ccaatttact gaccgtacac caaaatttgc ctgcattacc
3241 ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg gacatgttca gggatcgcca
3301 ggcgttttct gagcatacct ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg
3361 gtgcaagttg aataaccgga aatggtttcc cgcagaacct gaagatgttc gcgattatct
3421 tctatatctt caggcgcgcg gtctggcagt aaaaactatc cagcaacatt tgggccagct
3481 aaacatgctt catcgtcggt ccgggctgcc acgaccaagt gacagcaatg ctgtttcact
3541 ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct
3601 agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc atggaaaata gcgatcgctg
3661 ccaggatata cgtaatctgg catttctggg gattgcttat aacaccctgt tacgtatagc
3721 cgaaattgcc aggatcaggg ttaaagatat ctcacgtact gacggtggga gaatgttaat
3781 ccatattggc agaacgaaaa cgctggttag caccgcaggt gtagagaagg cacttagcct
3841 gggggtaact aaactggtcg agcgatggat ttccgtctct ggtgtagctg atgatccgaa
3901 taactacctg ttttgccggg tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca
3961 gctatcaact cgcgccctgg aagggatttt tgaagcaact catcgattga tttacggcgc
4021 taaggatgac tctggtcaga gatacctggc ctggtctgga cacagtgccc gtgtcggagc
4081 cgcgcgagat atggcccgcg ctggagtttc aataccggag atcatgcaag ctggtggctg
4141 gaccaatgta aatattgtca tgaactatat ccgtaacctg gatagtgaaa caggggcaat
4201 ggtgcgcctg ctggaagatg gcgattagaa acgttgttgt ttggggttga attactcttc
4261 tagaccgcgg cgcgcccgat aatcaacctc tggattacaa aatttgtgaa agattgactg
4321 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
4381 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
4441 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
4501 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
4561 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
4621 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
4681 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
4741 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
4801 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
4861 cctccccgca tcgataccgt cgacctcgat cgagacctag aaaaacatgg agcaatcaca
4921 agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag
4981 gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct
5041 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
5101 cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattgg
5161 cagaactaca caccagggcc agggatcaga tatccactga cctttggatg gtgctacaag
5221 ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa cacccgcttg
5281 ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtatt agagtggagg
5341 tttgacagcc gcctagcatt tcatcacatg gcccgagagc tgcatccgga ctgtactggg
5401 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
5461 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
5521 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagc
5581 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
5641 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
5701 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
5761 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
5821 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
5881 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
5941 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
6001 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
6061 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
6121 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
6181 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
6241 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
6301 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
6361 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
6421 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
6481 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
6541 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
6601 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
6661 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
6721 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
6781 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
6841 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
6901 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
6961 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
7021 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
7081 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
7141 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
7201 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
7261 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
7321 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
7381 gtgccacctg acg
//
