pCDH-puro-myr-HA-Akt1

LOCUS       pCDH-puro-myr-HA-Akt1                8965 bp ds-DNA     circular SYN 10-JAN-2018
DEFINITION  lentiviral expression of myr-HA-Akt1.
ACCESSION   .
VERSION     .
KEYWORDS    pCDH-puro-myr-HA-Akt1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8965)
  AUTHORS   Cheng Z, Gong Y, Ma Y, Lu K, Lu X, Pierce LA, Thompson RC, Muller S,
            Knapp S, Wang J
  TITLE     Inhibition of BET bromodomain targets genetically diverse 
            glioblastoma.
  JOURNAL   Clin Cancer Res. 2013 Apr 1;19(7):1748-59. doi: 
            10.1158/1078-0432.CCR-12-3066. Epub 2013 Feb 12.
  PUBMED    23403638
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTNAME|pCDH-puro-myr-HA-Akt1|
FEATURES             Location/Qualifiers
     source          1..8965
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(11..28)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(182..770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(262..281)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(941..1801)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1564..1583
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1802..1906)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1974..1992
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2030..2052)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2152..2171
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2365..2387
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2379..2396
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2380..2396
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2411..2637
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2638..2818
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2865..2990
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3483..3716
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    4212..4328
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4418..4621
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4572..4592
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4618..4642
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     4692..4711
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4692..4710
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        6324..6535
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             6548..6816
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             6843..7442
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6843..6862)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7339..7359
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7449..8037
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7502..7522)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7920..7931)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8111..8344
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8416..8537
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8453..8472)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8507..8526
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8577..8712
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8639..8658
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(8745..8761)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8745..8761)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8758..8780)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8769..8785
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8793..8823)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8838..8859
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     1921 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     1981 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     2041 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     2101 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
     2161 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
     2221 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     2281 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
     2341 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca
     2401 agctgacgcg tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt
     2461 tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg
     2521 tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc
     2581 actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg
     2641 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     2701 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     2761 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     2821 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     2881 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     2941 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     3001 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     3061 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     3121 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     3181 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     3241 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     3301 acaaaagtaa gaccaccgca cagcaagcgg ccactgatct tcagacctgg aggaggagat
     3361 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     3421 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     3481 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     3541 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     3601 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     3661 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     3721 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     3781 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     3841 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     3901 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     3961 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     4021 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     4081 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     4141 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     4201 tatcggttaa cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     4261 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaattc
     4321 aaaattttat cgatactagt attatgccca gtacatgacc ttatgggact ttcctacttg
     4381 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
     4441 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     4501 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     4561 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtttata taagcagagc
     4621 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
     4681 aagattctag ataatacgac tcactatagg gagacccaag cttcccacca ccatggggag
     4741 cagcaagagc aagcccaagg accccagcca gcgcgcctac gcctacgacg tgcccgacta
     4801 cgcctccctc ggatccatga gcgacgtggc tattgtgaag gagggttggc tgcacaaacg
     4861 aggggagtac atcaagacct ggcggccacg ctacttcctc ctcaagaatg atggcacctt
     4921 cattggctac aaggagcggc cgcaggatgt ggaccaacgt gaggctcccc tcaacaactt
     4981 ctctgtggcg cagtgccagc tgatgaagac ggagcggccc cggcccaaca ccttcatcat
     5041 ccgctgcctg cagtggacca ctgtcatcga acgcaccttc catgtggaga ctcctgagga
     5101 gcgggaggag tggacaaccg ccatccagac tgtggctgac ggcctcaaga agcaggagga
     5161 ggaggagatg gacttccggt cgggctcacc cagtgacaac tcaggggctg aagagatgga
     5221 ggtgtccctg gccaagccca agcaccgcgt gaccatgaac gagtttgagt acctgaagct
     5281 gctgggcaag ggcactttcg gcaaggtgat cctggtgaag gagaaggcca caggccgcta
     5341 ctacgccatg aagatcctca agaaggaagt catcgtggcc aaggacgagg tggcccacac
     5401 actcaccgag aaccgcgtcc tgcagaactc caggcacccc ttcctcacag ccctgaagta
     5461 ctctttccag acccacgacc gcctctgctt tgtcatggag tacgccaacg ggggcgagct
     5521 gttcttccac ctgtcccggg aacgtgtgtt ctccgaggac cgggcccgct tctatggcgc
     5581 tgagattgtg tcagccctgg actacctgca ctcggagaag aacgtggtgt accgggacct
     5641 caagctggag aacctcatgc tggacaagga cgggcacatt aagatcacag acttcgggct
     5701 gtgcaaggag gggatcaagg acggtgccac catgaagacc ttttgcggca cacctgagta
     5761 cctggccccc gaggtgctgg aggacaatga ctacggccgt gcagtggact ggtgggggct
     5821 gggcgtggtc atgtacgaga tgatgtgcgg tcgcctgccc ttctacaacc aggaccatga
     5881 gaagcttttt gagctcatcc tcatggagga gatccgcttc ccgcgcacgc ttggtcccga
     5941 ggccaagtcc ttgctttcag ggctgctcaa gaaggacccc aagcagaggc ttggcggggg
     6001 ctccgaggac gccaaggaga tcatgcagca tcgcttcttt gccggtatcg tgtggcagca
     6061 cgtgtacgag aagaagctca gcccaccctt caagccccag gtcacgtcgg agactgacac
     6121 caggtatttt gatgaggagt tcacggccca gatgatcacc atcacaccac ctgaccaaga
     6181 tgacagcatg gagtgtgtgg acagcgagcg caggccccac ttcccccagt tctcctactc
     6241 ggccagcggc acggcctgag aattcgaatt taaatcggat ccgcggccgc gaaggatctg
     6301 cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     6361 ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga
     6421 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     6481 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa
     6541 gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc
     6601 acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc
     6661 cgtctaggta agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag
     6721 cctacctaga ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt
     6781 ctttgtttcg ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct
     6841 agatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg
     6901 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg
     6961 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
     7021 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg
     7081 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
     7141 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
     7201 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg
     7261 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
     7321 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
     7381 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct
     7441 gagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     7501 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     7561 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     7621 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     7681 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     7741 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     7801 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     7861 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     7921 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     7981 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
     8041 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     8101 aaggggggac tggaagggct aattcactcc caacgaagat aagatctgct ttttgcttgt
     8161 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     8221 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     8281 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     8341 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     8401 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     8461 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     8521 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc
     8581 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     8641 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     8701 tttttggagg cctagacttt tgcagagacc aaattcgtaa tcatgtcata gctgtttcct
     8761 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     8821 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     8881 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     8941 agaggcggtt tgcgtattgg gcgct
//