pSJX016

LOCUS       pSJX016         14084 bp ds-DNA     circular SYN 20-DEC-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Dec 23, 2024 from SnapGene 4.3.6
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..14084
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    458..1252
                     /standard_name="kanamycin resistance determinant"
                     /label=neo
                     /label=KanR
     misc_feature    1580..1614
                     /label=PJ23119
     misc_feature    1615..1634
                     /label=sgRNA2
     misc_feature    1635..1717
                     /label=gRNA scaffold
     misc_feature    1718..3717
                     /label=UA
     gene            1718..1824
                     /locus_tag="CCNA_02257"
                     /label=CCNA_02257
                     /db_xref="GeneID:7332622"
     gene            1910..1994
                     /locus_tag="CCNA_R0052"
                     /label=CCNA_R0052
                     /db_xref="GeneID:7332621"
     gene            2143..3612
                     /locus_tag="CCNA_02256"
                     /label=CCNA_02256
                     /db_xref="GeneID:7332620"
                     /experiment="gene is essential according to Tn-seq 
                     experiments of Christen, et al. (2011) Mol Syst Biol. 
                     30;7:528. doi: 10.1038/msb.2011.58"
     misc_feature    3718..5717
                     /label=DA
     gene            3919..5079
                     /locus_tag="CCNA_02254"
                     /label=CCNA_02254
                     /db_xref="GeneID:7332618"
     gene            complement(5188..5274)
                     /locus_tag="CCNA_03965"
                     /label=CCNA_03965
                     /db_xref="GeneID:18668899"
     gene            complement(5330..5717)
                     /locus_tag="CCNA_02253"
                     /label=CCNA_02253
                     /db_xref="GeneID:7332616"
     misc_feature    5738..5797
                     /label=rrnB T1T2
     misc_feature    5798..5920
                     /label=Pvan
                     /label=PvanR-PvanA
     CDS             5924..10027
                     /codon_start=1
                     /label=SpCas9M
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     primer_bind     complement(10010..10037)
                     /label=SJX1577
     primer_bind     10018..10063
                     /label=SJX1580
     misc_feature    10028..10042
                     /label=HRSAT-linker
     CDS             10043..10759
                     /codon_start=1
                     /label=sfgfp
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(10730..10769)
                     /label=SJX1582
     primer_bind     10750..10779
                     /label=SJX1581
     misc_feature    11630..12292
                     /standard_name="rep"
                     /label=rep
ORIGIN
        1 accttcggga gcgcctgaag cccgttctgg acgccctggg gccgttgaat cgggatatgc
       61 aggccaaggc cgccgcgatc atcaaggccg tgggcgaaaa gctgctgacg gaacagcggg
      121 aagtccagcg ccagaaacag gcccagcgcc agcaggaacg cgggcgcgca catttccccg
      181 aaaagtgcca cctgggatga atgtcagcta ctgggctatc tggacaaggg aaaacgcaag
      241 cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag actgggcggt
      301 tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa
      361 gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc gcaggggatc
      421 aagatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
      481 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
      541 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
      601 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
      661 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
      721 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
      781 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
      841 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
      901 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
      961 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     1021 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     1081 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     1141 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     1201 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     1261 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     1321 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     1381 atcctccagc gcggggatct catgctggag ttcttcgccc acccccatgg gcaaatatta
     1441 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
     1501 tggcttccat gtcggcagaa tgcttaatga attacaacag tttttatata agctttcagt
     1561 cggcgcgaat gctccacgct tgacagctag ctcagtccta ggtataatac tagttctctt
     1621 gatgagatcg acgggtttta gagctagaaa tagcaagtta aaataaggct agtccgttat
     1681 caacttgaaa aagtggcacc gagtcggtgc tttttttaca atcacccgat cgcccgcggc
     1741 gaaatccaga tcagcgacga tcgcatcgct atccaggtga cccgcgcggc tgacgtctac
     1801 gactacatgg ccggcggcag ctgatccgtc gcgggcttga gttcgacatt ctcgaacttc
     1861 cggcttccca aagccgattc agtttgctac cagccgcccc tcaccacgag cggtcgtggc
     1921 ggaattggta gacgcgcagc gttgaggtcg ctgtggggca acccgtggaa gttcgagtct
     1981 tctcgaccgc accaaggtga acaagagtag ggcgcgcttg gcgaaatcgg tttccgactc
     2041 gctgggggcg ctctaatcgt ttctggccct gacttcgcct cagggtcgct tgatcggacc
     2101 agcccaacga acccacgcgc tctgagagct caggaggccc ccatgacccg ggacaccgcc
     2161 gaactgaacg aagacgtgct ggacaacatc ccgctggccc atatcccgct tcccaagtcc
     2221 gagacgcccg aggagctgga agacctcgcc ctcggcgacg actacgcgct taatcccgaa
     2281 tatgtcgaga tggtgatcga cgccgccgac cgcggcgacg ccaagcgcct gcgcgagctg
     2341 gtgggggccc tggaccccgc cgacgtcgcc gacctgatgg gctttctgac ggccgactat
     2401 cgcgaggaag tgatcccgct gctcgacccc gaggcgctgg gcgagatcat ttccgagctg
     2461 gaggacaaca tccgcgagga agtgctggag gccaccccgt cggtgaccct ggcccgggcc
     2521 ctcgaggagc tggacaccga cgacgccgcc gacgttatcg acgacctgga cgacaccaag
     2581 cgcttccagg tgctggccgc catgggcgag accgaccgcg ccgccatcga gacgaccctg
     2641 tcctacgagg acgagaccgc cggccgcctg atgcagcgcg agttcctggc cgcgccggaa
     2701 ttctggaccg tcggccagac gatcgaccat gtccgcgagg ccggcgactc gctgcccgag
     2761 ctgttcttcg acgtctatgt cgtcgacccg accttcaagc ctgtcggggc gatcccgatc
     2821 agcatcctgc tgcgcagccg tcgtcagacg ccgctggccg agctgatgga ggcggtcacc
     2881 gaaatcccgg tcgagatgga ccaggaagag gtcgcctaca ttttcgacaa gtaccacctg
     2941 atcagcgccc cggtgattga accgggcggg cggctggtgg gtcagatcac tgtcgacgac
     3001 atcgtcgagg ttatccgcga cgaggccgag gaagacattc tggccctggc cggtgtgtcg
     3061 gacgccggcc gtgacgcctc ggtgatcgag atcgtcaagt cgcgcctgcc gtggctgatg
     3121 ctgaacctgg cgaccgccac cctggccgtc agcggcgtgg ccatcttcca ggccgagatc
     3181 gctcaattgg tggccctggc catcctgatg ccgatcgtct cgtccctggg cggcaacgcc
     3241 ggcacccaga ccctggccgt ggccgtgcgg gctctggcca gcaaggagct gacccccgcc
     3301 aacgcccgcc gcatcgtggc gcgggaactg ctagtgggcc tggttaacgg gctttgcctg
     3361 gcgctggtga tgggggcggc gacctacatg ttcttcgggc cgtcggatca acacgacaag
     3421 ctggcgatca tcgtcggcgc ggccctgatc accaacatct tcaccgcggc cctgggcggc
     3481 atcctgatgc ccctggcgct ggagaagatg ggccgtgacc cggcggtgtc gtcctcgatc
     3541 ttcgtcacct tcctgaccga cttcaccggc ttcttcgcgg tgctgctgat cgcctcgctg
     3601 ctgctgcact gaggttttct cgctggccac gaccctcgcc aggggaaact ggcgccgacc
     3661 ttgcgccggg acaggcgaac gcatccgcgt ctgtctcaaa ctgggtcaat ctaggcggtt
     3721 tcttgcctcg ccaccccgag gcatgagcaa gaacgcccgc tggtcccaaa gactggcggg
     3781 cgttttttgc tatcgtgcgg ctgttttctg gtggaaatcg accgagatcg gatgcacacg
     3841 ctctctcagt gttgagccag agcgtgacaa gcgctgaaac cgcccacatc ttccgctcgg
     3901 gcgttcggga aggaaaccat gtcgatgact ttcgcaccct cgatggactt cgcgctgggc
     3961 gagaccgctg acgcgatccg tgagaccacg gcccgtttcg ccgccgacaa gatcgcaccg
     4021 atcgccgcca agatcgacga gaccaacagc ttcccccgcg aactctgggt cccgatgggg
     4081 gatctgggtc tgcatggcat caccgtcgag gaagagttcg gggggctggg tctgggctat
     4141 ctcgaacacg tcgtggcgat ggaagaggtc agccgcgcct ccgcctccgt ggggctcagc
     4201 tacggcgccc actccaatct ctgcgtgaac cagatccgtc ggtgggccac gccggagcag
     4261 aaggcgcgct atctgcccaa gctgatcagc ggcgagcatg tgggctccct ggcgatgagc
     4321 gaagccggcg cgggctctga tgtcgtctcg atgaagctgc gcgccgagca ggtgggcgac
     4381 cgctacatcc tcaacggcac gaagttctgg atcaccaacg ccccgcacgc cgatacgctg
     4441 gtggtctacg ccaagaccgg cgagggcagc cgcggcatca ccgccttcat cgtcgagaag
     4501 ggcatgaagg gcttcagcgt ctccaagaag ctggacaaga tgggcatgcg cgggtcggac
     4561 accgccgagc tggtgttcga agactgcgag atccccgaag agaacgtcat gggcccggtc
     4621 ggcggtggcg tcggggtgct gatgagcggc ctcgactacg agcgggccgt gctggcggcc
     4681 ggtccgctgg gcatcatgca ggcttgcctc gatgtcgtgc tgccctacgt ccgcgaccgc
     4741 aagcagttcg gccagccgat cggctcgttc cagctgatgc agggcaagat cgccgacatg
     4801 tacgtggccc tgaactcggc ccgggcttac gtctacgcgg tggccaaggc ttgtgacgcg
     4861 ggcaagacca cgcgcttcga cgccgccggc gcgatcctga tggcgtcgga aaacgccgtg
     4921 aaggtgtcgc tggaggcgat ccaggccctc ggtggcgctg gctataccaa ggagtggccg
     4981 gtcgagcgcc tgctgcgcga cgccaagctc tatgacatcg gcgccggcac caacgagatc
     5041 cgccgcttcc tgatcggacg cgagctgatc ggcggctgat cctgggctgc gcgacatcaa
     5101 aagggccgcc ccgatcgctc ggggcggccc tccatcgtcg tagccgctct cgcagccagt
     5161 gactcgaaac ccttggcgct caacgcctta ggcggagacc tcgctcttcg ggctccaggt
     5221 cacgacttgc gaccgcggtt ggagacaatg agacactgga ccacctcctt tcagttgttg
     5281 aacaacacag gaaggatatt ggaccggttt tcgcgaaagg aaagggtcgt tatcgcgacc
     5341 aagcgggact aaacgccgcg cttcgctcgg ccaggctggg ccgcttgcga agatcggtga
     5401 tggggcgatg taccccgtcg agggcgaagc cggcggcgac gtagccggcc ttcacggcgg
     5461 gttcgaaggc gcgccagtca cggatatcga tgccttccag cttcggggtg atcagaacgt
     5521 cggtcgcctc gcgagcggcg gccagatcgc gcccggtcgt gacggtcgca gcacgcatca
     5581 gcaaggccac gatcggcggg cccttgcgcc attcgccgga gagaatccag ttccacaggg
     5641 atgggggcgt gacgatgtca gcgctggtga tgctgcggcc acgggtgacg tcgaccccga
     5701 cgatcggtcc aagctggcgc gcccagcggc gcggatccaa ataaaacgaa aggctcagtc
     5761 gaaagactgg gcctttcgtt ttatctgttg tttgtcgggc tggactctag ccgaccgact
     5821 gagacgctca caagcgttca attggatcca atcttgacgt ccgtttgatt acgatcaaga
     5881 ttggatccaa taggtcgccg aaccacgatg cgaggaaacg catatggaca agaagtactc
     5941 gatcggcctg gatatcggga cgaacagcgt cgggtgggcc gtgatcacgg acgaatacaa
     6001 ggtgccctcc aagaagttca aggtcctggg gaataccgac cggcactcca tcaagaagaa
     6061 cctcatcggg gccctgctct ttgactccgg ggaaacggcc gaagcgacgc ggctgaagcg
     6121 gaccgcgcgt cgtcgctaca cgcgccggaa gaatcgcatc tgttatctcc aggaaatctt
     6181 cagcaacgag atggccaagg tggatgattc cttcttccac cgcctggagg agtcctttct
     6241 ggtcgaagag gataagaagc atgagcgcca cccgatcttt gggaacatcg tcgacgaagt
     6301 ggcctaccac gaaaagtatc ccacgatcta ccacctgcgt aagaagctcg tggactccac
     6361 cgataaggcg gatctgcggc tgatctacct cgccctggcc cacatgatca agtttcgggg
     6421 ccattttctg atcgaaggcg atctgaatcc ggacaatagc gacgtcgata agctctttat
     6481 ccagctggtg cagacctaca atcaactctt cgaggagaat ccgatcaatg cctccggggt
     6541 ggatgccaag gcgatcctga gcgcgcgtct gtcgaagtcc cgccgcctgg agaacctgat
     6601 cgcccagctc ccgggcgaaa agaagaacgg gctctttggg aatctgatcg ccctcagcct
     6661 ggggctgacc ccgaacttta agtccaattt cgacctcgcg gaagatgcca agctccaact
     6721 ctcgaaggat acgtacgacg atgacctcga caacctgctg gcccaaatcg gcgaccagta
     6781 cgcggacctg ttcctggcgg ccaagaacct gtcggacgcc atcctcctga gcgacatcct
     6841 ccgtgtcaat acggaaatca cgaaggcgcc gctgtccgcc agcatgatca agcggtatga
     6901 tgagcatcat caagacctca ccctgctgaa ggcgctggtc cggcaacagc tgcccgagaa
     6961 gtacaaggag atctttttcg atcaaagcaa gaacggctac gccgggtata tcgacggggg
     7021 ggcctcgcaa gaagagttct acaagtttat caagcccatc ctggagaaga tggatgggac
     7081 ggaggagctc ctcgtcaagc tcaaccggga agatctcctc cgcaagcaac gcacctttga
     7141 taatgggtcc atcccgcatc aaatccacct cggcgagctc catgcgatcc tgcggcggca
     7201 agaggacttc tacccgtttc tcaaggacaa ccgcgagaag atcgagaaga tcctgacgtt
     7261 ccgcatcccc tattatgtcg gccccctggc ccgggggaat tcgcggtttg cctggatgac
     7321 ccgcaagtcc gaagaaacga tcaccccctg gaattttgaa gaggtggtgg acaagggggc
     7381 gagcgcgcag tcctttatcg aacggatgac caatttcgac aagaacctcc ccaacgagaa
     7441 ggtgctcccg aagcattccc tcctgtatga gtactttacc gtctacaacg agctcacgaa
     7501 ggtcaagtat gtcaccgagg gcatgcgcaa gcccgccttc ctctcggggg aacaaaagaa
     7561 ggccatcgtg gacctgctct tcaagaccaa ccgcaaggtc accgtgaagc aactcaagga
     7621 ggactatttc aagaagatcg aatgcttcga tagcgtggag atctccgggg tcgaagaccg
     7681 gtttaacgcc tcgctcggca cgtaccatga tctcctcaag atcatcaagg acaaggactt
     7741 cctcgacaac gaggagaacg aagacatcct ggaagacatc gtgctgaccc tgaccctctt
     7801 cgaggatcgc gagatgatcg aggagcgtct caagacgtac gcgcatctct tcgatgataa
     7861 ggtgatgaag caactcaagc ggcggcgtta cacgggctgg gggcgcctct cccgtaagct
     7921 catcaacggc atccgggaca agcaatcggg gaagaccatc ctggacttcc tgaagtcgga
     7981 cggctttgcc aatcgcaatt ttatgcaact catccacgac gattccctca ccttcaagga
     8041 agatatccag aaggcccagg tgtccgggca aggggactcc ctgcatgaac atatcgccaa
     8101 cctggccggg agccccgcga tcaagaaggg gatcctccag accgtgaagg tggtcgatga
     8161 gctcgtgaag gtcatgggcc ggcataagcc cgaaaatatc gtcatcgaga tggcgcgcga
     8221 gaaccagacc acccaaaagg gccagaagaa ttcgcgggag cgcatgaagc ggatcgaaga
     8281 aggcatcaag gaactcggca gccagatcct gaaggaacat ccggtcgaaa acacgcagct
     8341 ccagaacgag aagctctacc tctactatct ccaaaatggc cgggatatgt acgtggacca
     8401 ggagctcgac atcaatcggc tcagcgacta tgatgtggat catatcgtcc cccaatcctt
     8461 tctcaaggac gacagcatcg ataacaaggt cctgacccgg tcggacaaga atcgtggcaa
     8521 gagcgataac gtcccctccg aggaggtcgt gaagaagatg aagaactatt ggcgccaact
     8581 gctcaacgcc aagctgatca cgcaacggaa gttcgacaac ctcaccaagg ccgagcgcgg
     8641 gggcctctcg gaactggaca aggcggggtt catcaagcgc cagctcgtcg aaacccgtca
     8701 aatcaccaag cacgtggcgc aaatcctgga tagccgcatg aacaccaagt acgatgagaa
     8761 cgacaagctg atccgtgaag tgaaggtgat caccctgaag tccaagctcg tgagcgattt
     8821 ccgcaaggat ttccaatttt ataaggtgcg ggaaatcaat aattaccatc acgcgcatga
     8881 tgcgtatctc aacgccgtgg tcggcacggc cctgatcaag aagtatccca agctggagtc
     8941 ggaattcgtc tacggcgatt acaaggtgta tgacgtccgt aagatgatcg cgaagagcga
     9001 gcaagaaatc gggaaggcca cggcgaagta cttcttctac agcaatatca tgaatttctt
     9061 caagacggag atcacgctgg cgaatggcga aatccgtaag cgcccgctga tcgaaacgaa
     9121 tggcgagacc ggcgaaatcg tgtgggataa ggggcgcgat ttcgcgacgg tccgcaaggt
     9181 cctctcgatg ccgcaggtca acatcgtcaa gaagaccgaa gtccagaccg gggggttttc
     9241 caaggagagc atcctgccga agcgcaacag cgacaagctc atcgcccgca agaaggactg
     9301 ggatcccaag aagtatggcg ggttcgatag cccgaccgtg gcgtactcgg tgctggtggt
     9361 cgcgaaggtc gaaaagggga agtccaagaa gctcaagagc gtcaaggaac tgctggggat
     9421 cacgatcatg gaacgctcct cctttgagaa gaatcccatc gacttcctgg aggcgaaggg
     9481 ctataaggag gtgaagaagg atctcatcat caagctgccg aagtattcgc tcttcgaact
     9541 cgaaaatggg cgcaagcgca tgctggcgtc cgccggcgag ctccaaaagg gcaatgagct
     9601 ggccctgccc tccaagtacg tcaattttct gtacctagcc tcccactatg agaagctgaa
     9661 gggcagcccc gaggataatg aacagaagca actctttgtc gagcaacata agcattacct
     9721 ggatgaaatc atcgagcaaa tcagcgaatt ttccaagcgg gtgatcctcg ccgatgccaa
     9781 cctggacaag gtcctcagcg cgtataacaa gcaccgggac aagcccatcc gtgaacaagc
     9841 ggagaacatc atccatctgt tcacgctcac caacctgggc gcccccgccg cgtttaagta
     9901 ttttgatacc accatcgatc gcaagcgtta tacgtccacc aaggaagtcc tcgatgccac
     9961 gctgatccat caaagcatca ccgggctcta cgagacccgg atcgatctct cccagctggg
    10021 cggcgatcac cggtcggcca ccatgcgtaa aggcgaagag ctgttcactg gtgtcgtccc
    10081 tattctggtg gaactggatg gtgatgtcaa cggtcataag ttttccgtgc gtggcgaggg
    10141 tgaaggtgac gcaactaatg gtaaactgac gctgaagttc atctgtacta ctggtaaact
    10201 gccggtacct tggccgactc tggtaacgac gctgacttat ggtgttcagt gctttgctcg
    10261 ttatccggac catatgaagc agcatgactt cttcaagtcc gccatgccgg aaggctatgt
    10321 gcaggaacgc acgatttcct ttaaggatga cggcacgtac aaaacgcgtg cggaagtgaa
    10381 atttgaaggc gataccctgg taaaccgcat tgagctgaaa ggcattgact ttaaagaaga
    10441 cggcaatatc ctgggccata agctggaata caattttaac agccacaatg tttacatcac
    10501 cgccgataaa caaaaaaatg gcattaaagc gaattttaaa attcgccaca acgtggagga
    10561 tggcagcgtg cagctggctg atcactacca gcaaaacact ccaatcggtg atggtcctgt
    10621 tctgctgcca gacaatcact atctgagcac gcaaagcgtt ctgtctaaag atccgaacga
    10681 gaaacgcgat catatggttc tgctggagtt cgtaaccgca gcgggcatca cgcatggtat
    10741 ggatgaactg tacaaatgaa tgaaaacggg ccccccctcg aggtcgacgg tatcgataag
    10801 cttgatatcg aattcctgca gcccggggga tccactagtt ctagagcggc cgccaccgcg
    10861 gtggagctcc aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt
    10921 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
    10981 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
    11041 gcgcagcctg aatggcgaat ggaaattgta agcgttaata ttttgttaaa attcgcgtta
    11101 aatttttgtt aaatcagctc attttttaac caataggccg tactgcgatg agtggcaggg
    11161 cggggcgtaa tttttttaag gcagttattg gtgcccttaa acgcctggtg ctacgcctga
    11221 ataagtgata ataagcggat gaatggcaga aattcgaaag caaattcgac ccggtcgtcg
    11281 gttcagggca gggtcgttaa atagccgctt atgtctattg ctggtttacc ggtttattga
    11341 ctaccggaag cagtgtgacc gtgtgcttct caaatgcctg aggccagttt gctcaggctc
    11401 tccccgtgga ggtaataatt gacgatatga tcatttattc tgcctcccag agcctgataa
    11461 aaacggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct
    11521 ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcga ggcggctaca
    11581 gccgatagtc tggaacagcg cacttacggg ttgctgcgca acccaagtgc taccggcgcg
    11641 gcagcgtgac ccgtgtcggc ggctccaacg gctcgccatc gtccagaaaa cacggctcat
    11701 cgggcatcgg caggcgctgc tgcccgcgcc gttcccattc ctccgtttcg gtcaaggctg
    11761 gcaggtctgg ttccatgccc ggaatgccgg gctggctggg cggctcctcg ccggggccgg
    11821 tcggtagttg ctgctcgccc ggatacaggg tcgggatgcg gcgcaggtcg ccatgcccca
    11881 acagcgattc gtcctggtcg tcgtgatcaa ccaccacggc ggcactgaac accgacaggc
    11941 gcaactggtc gcggggctgg ccccacgcca cgcggtcatt gaccacgtag gccgacacgg
    12001 tgccggggcc gttgagcttc acgacggaga tccagcgctc ggccaccaag tccttgactg
    12061 cgtattggac cgtccgcaaa gaacgtccga tgagcttgga aagtgtcttc tggctgacca
    12121 ccacggcgtt ctggtggccc atctgcgcca cgaggtgatg cagcagcatt gccgccgtgg
    12181 gtttcctcgc aataagcccg gcccacgcct catgcgcttt gcgttccgtt tgcacccagt
    12241 gaccgggctt gttcttggct tgaatgccga tttctctgga ctgcgtggcc atgcttatct
    12301 ccatgcggta gggtgccgca cggttgcggc accatgcgca atcagctgca acttttcggc
    12361 agcgcgacaa caattatgcg ttgcgtaaaa gtggcagtca attacagatt ttctttaacc
    12421 tacgcaatga gctattgcgg ggggtgccgc aatgagctgt tgcgtacccc ccttttttaa
    12481 gttgttgatt tttaagtctt tcgcatttcg ccctatatct agttctttgg tgcccaaaga
    12541 agggcacccc tgcggggttc ccccacgcct tcggcgcggc tccccctccg gcaaaaagtg
    12601 gcccctccgg ggcttgttga tcgactgcgc ggccttcggc cttgcccaag gtggcgctgc
    12661 ccccttggaa cccccgcact cgccgccgtg aggctcgggg ggcaggcggg cgggcttcgc
    12721 cttcgactgc ccccactcgc ataggcttgg gtcgttccag gcgcgtcaag gccaagccgc
    12781 tgcgcggtcg ctgcgcgagc cttgacccgc cttccacttg gtgtccaacc ggcaagcgaa
    12841 gcgcgcaggc cgcaggccgg aggcttttcc ccagagaaaa ttaaaaaaat tgatggggca
    12901 aggccgcagg ccgcgcagtt ggagccggtg ggtatgtggt cgaaggctgg gtagccggtg
    12961 ggcaatccct gtggtcaagc tcgtgggcag gcgcagcctg tccatcagct tgtccagcag
    13021 ggttgtccac gggccgagcg aagcgagcca gccggtggcc gctcgcggcc atcgtccaca
    13081 tatccacggg ctggcaaggg agcgcagcga ccgcgcaggg cgaagcccgg agagcaagcc
    13141 cgtagggcgc cgcagccgcc gtaggcggtc acgactttgc gaagcaaagt ctagtgagta
    13201 tactcaagca ttgagtggcc cgccggaggc accgccttgc gctgcccccg tcgagccggt
    13261 tggacaccaa aagggagggg caggcatggc ggcatacgcg atcatgcgat gcaagaagct
    13321 ggcgaaaatg ggcaacgtgg cggccagtct caagcacgcc taccgcgagc gcgagacgcc
    13381 caacgctgac gccagcagga cgccagagaa cgagcactgg gcggccagca gcaccgatga
    13441 agcgatgggc cgactgcgcg agttgctgcc agagaagcgg cgcaaggacg ctgtgttggc
    13501 ggtcgagtac gtcatgacgg ccagcccgga atggtggaag tcggccagcc aagaacagca
    13561 ggcggcgttc ttcgagaagg cgcacaagtg gctggcggac aagtacgggg cggatcgcat
    13621 cgtgacggcc agcatccacc gtgacgaaac cagcccgcac atgaccgcgt tcgtggtgcc
    13681 gctgacgcag gacggcaggc tgtcggccaa ggagttcatc ggcaacaaag cgcagatgac
    13741 ccgcgaccag accacgtttg cggccgctgt ggccgatcta gggctgcaac ggggcatcga
    13801 gggcagcaag gcacgtcaca cgcgcattca ggcgttctac gaggccctgg agcggccacc
    13861 agtgggccac gtcaccatca gcccgcaagc ggtcgagcca cgcgcctatg caccgcaggg
    13921 attggccgaa aagctgggaa tctcaaagcg cgttgagacg ccggaagccg tggccgaccg
    13981 gctgacaaaa gcggttcggc aggggtatga gcctgcccta caggccgccg caggagcgcg
    14041 tgagatgcgc aagaaggccg atcaagccca agagacggcc cgag
//