| 质粒名称: | pDG210 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002909 |
| 产品用途: | Mammalian PhCMV-driven constitutive expression vector for UbVR-tagged NanoLuc (PhCMV-UbVR-NanoLuc-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 未拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | UbVR, NanoLuc |
| 物种来源: | synthetic |
| 密码子优化: | 未优化 |
pDG210
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pDG210载体质粒基本信息
pDG210质粒图谱载体图谱和pDG210载体序列质粒序列多克隆位点信息
pDG210质粒载体简介
pDG210质粒序列载体序列
LOCUS pDG210 6260 bp DNA circular SYN 21-JUL-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6260)
AUTHORS Westlake University
TITLE Direct Submission
JOURNAL Exported Sep 11, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6260
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 826..845
/label=Primer 1
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 887..944
/label=oSCL1108
misc_feature 895..911
/label=MCS
/note="multiple cloning site"
primer_bind 897..944
/label=oSCL1314
primer_bind 898..936
/label=Primer 14
primer_bind 898..931
/label=Primer 7a
primer_bind 899..936
/label=Primer 7
primer_bind 899..936
/label=Primer 16
primer_bind 899..936
/label=Primer 26
primer_bind complement(899..919)
/label=Primer 25
primer_bind 911..936
/label=Primer 1a
primer_bind 911..936
/label=Primer 9
promoter 911
/label=P_SV40
primer_bind 917..944
/label=oSCL1846
primer_bind 917..940
/label=oSCL1848
primer_bind 917..936
/label=Primer 3
primer_bind 921..944
/label=oSCL084
misc_feature 923..991
/label=Secretory (IgK)
/label=nonstandard type: signal
primer_bind 990..1030
/label=Primer 9a
primer_bind complement(990..1009)
/label=Primer 8
CDS 1010..1243
/codon_start=1
/transl_table=11
/product="UbR-tTA-Dendra2"
/label=UbR-tTA-Dendra2
/protein_id="QCO92762.1"
/translation="MTSQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRL
IFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
primer_bind 1010..1046
/label=Primer 5
primer_bind 1010..1025
/label=Primer 7a
misc_feature 1019..1243
/label=UbR
/note="UbR"
primer_bind 1027..1085
/label=Primer 3a
primer_bind 1066..1124
/label=Primer 1`b
primer_bind complement(1105..1163)
/label=Primer 2a
primer_bind 1126..1137
/label=Primer 5
primer_bind complement(1144..1202)
/label=Primer 4
primer_bind complement(1183..1237)
/label=Primer 6
primer_bind complement(1218..1267)
/label=Primer 8a
primer_bind complement(1218..1267)
/label=Primer 10
primer_bind complement(1224..1269)
/label=Primer 11
primer_bind complement(1224..1269)
/label=Primer 12
primer_bind complement(1224..1269)
/label=Primer 19
primer_bind complement(1224..1269)
/label=Primer 13
primer_bind complement(1224..1269)
/label=Primer 21
primer_bind complement(1224..1269)
/label=Primer 22
primer_bind complement(1224..1269)
/label=Fragment.REV
primer_bind complement(1224..1267)
/label=Primer 20
primer_bind complement(1224..1267)
/label=Primer 23
primer_bind complement(1224..1267)
/label=Primer 24
primer_bind 1250..1290
/label=Primer 5a
primer_bind 1250..1287
/label=Primer 17
primer_bind complement(1250..1267)
/label=Primer 15
misc_feature 1271..1780
/label=NLuc (nano Luciferase)
/label=nonstandard type: reporter
misc_feature 1301..1303
/label=cyt-STOP (TGG->TGA)
primer_bind complement(1760..1783)
/label=oSCL1849
primer_bind complement(1761..1828)
/label=Primer 10a
primer_bind complement(1761..1801)
/label=Primer 18
primer_bind complement(1761..1800)
/label=Primer 6a
primer_bind complement(1761..1783)
/label=Primer 4a
primer_bind complement(1761..1780)
/label=Primer 15
primer_bind complement(1761..1780)
/label=Primer 27
primer_bind complement(1770..1780)
/label=oSCL085
primer_bind complement(1770..1780)
/label=oSCL1109
misc_feature 1789..1834
/label=MCS
/note="multiple cloning site"
primer_bind complement(1834..1853)
/label=Primer 2
polyA_signal 1860..2084
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 2130..2558
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2572..2901
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 2752..2887
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 2887..2906
/label=Primer 1a
CDS 2968..3762
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin)"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 3936..4057
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(4508..5093)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5264..6124)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(6125..6229)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagcttgcca ccatgactag tgagacagac acactcctgc tatgggtact
961 gctgctctgg gttccaggtt ccactggtga cgctagtggt ggttctggta tgactagtca
1021 gattttcgtg aagaccctga ccggcaagac catcacccta gaggtggagc ccagtgacac
1081 catcgagaac gtgaaggcca agatccagga taaagagggc atcccccctg accagcagag
1141 gctgatcttt gccggcaagc agctggaaga tggccgcacc ctctctgatt acaacatcca
1201 gaaggagtca accctgcacc tggtccttcg cctgagaggt ggcgttcgca gcggaagtgg
1261 aagcagtatg gtcttcacac tcgaagattt cgttggggac tggcgacaga cagccggcta
1321 caacctggac caagtccttg aacagggagg tgtgtccagt ttgtttcaga atctcggggt
1381 gtccgtaact ccgatccaaa ggattgtcct gagcggtgaa aatgggctga agatcgacat
1441 ccatgtcatc atcccgtatg aaggtctgag cggcgaccaa atgggccaga tcgaaaaaat
1501 ttttaaggtg gtgtaccctg tggatgatca tcactttaag gtgatcctgc actatggcac
1561 actggtaatc gacggggtta cgccgaacat gatcgactat ttcggacggc cgtatgaagg
1621 catcgccgtg ttcgacggca aaaagatcac tgtaacaggg accctgtgga acggcaacaa
1681 aattatcgac gagcgcctga tcaaccccga cggctccctg ctgttccgag taaccatcaa
1741 cggagtgacc ggctggcggc tgtgcgaacg cattctggcg tgagaattct gcagatatcc
1801 agcacagtgg cggccgctcg agtctagagg gcccgtttaa acccgctgat cagcctcgac
1861 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
1921 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
1981 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
2041 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
2101 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
2161 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
2221 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
2281 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
2341 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
2401 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
2461 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
2521 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
2581 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
2641 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
2701 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
2761 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
2821 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
2881 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
2941 caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct
3001 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
3061 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
3121 gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc
3181 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
3241 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
3301 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
3361 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
3421 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
3481 gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
3541 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
3601 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
3661 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
3721 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg
3781 aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct
3841 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
3901 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
3961 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
4021 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
4081 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
4141 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
4201 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
4261 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
4321 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
4381 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
4441 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
4501 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
4561 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
4621 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
4681 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
4741 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
4801 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
4861 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
4921 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
4981 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
5041 gcggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
5101 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
5161 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
5221 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
5281 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
5341 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
5401 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
5461 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
5521 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
5581 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
5641 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
5701 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
5761 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
5821 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
5881 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
5941 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
6001 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
6061 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
6121 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
6181 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
6241 gaaaagtgcc acctgacgtc
//
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