| 出品公司: | 爱迪基因 |
|---|---|
| 载体名称: | pAAV-CMV-iRFP |
| 质粒类型: | 腺病毒载体；荧光蛋白报告载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | CMV |
| 载体大小: | 5603 bp |
| 5' 测序引物及序列: | pCAX-F (CAGCTCCTGGGCAACGTGC) |
| 3' 测序引物及序列: | C-CMV-24 (TATTAGGACAAGGCTGGTGGGCAC) |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 红外荧光蛋白iRFP |
| 克隆菌株: | Stbl3 或 NEB Stble |
| 宿主细胞（系）: | 常规哺乳动物细胞系 |
| 备注: | -- |
| 产品目录号: | #64887 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 腺病毒 |
买家导航
pAAV-CMV-iRFP载体质粒基本信息
pAAV-CMV-iRFP质粒图谱载体图谱和pAAV-CMV-iRFP载体序列质粒序列多克隆位点信息
pAAV-CMV-iRFP质粒载体简介
pAAV-CMV-iRFP质粒序列载体序列
LOCUS pAAV-CMV-iRFP Sequencing Result 5603 bp DNA SYN
DEFINITION pAAV-CMV-iRFP Sequencing Result
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|pAAV-CMV-iRFP|
FEATURES Location/Qualifiers
source 1..5603
/organism="pAAV-CMV-iRFP Sequencing Result"
/mol_type="other DNA"
promoter 159..710
/label="CMV_immearly_promoter"
misc_feature 214..501
/label="CAG_enhancer"
misc_feature 667..687
/label="CMV_fwd_primer"
promoter 668..737
/label="CMV_promoter"
promoter 680..799
/label="CMV2_promoter"
misc_feature 711..730
/label="pCEP_fwd_primer"
misc_feature 713..737
/label="LNCX_primer"
misc_feature 809..1301
/label="bGlob_int"
CDS 1341..2291
/label="ORF frame 3"
/translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAG
SDNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAG
FIGSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVR
KITGFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRI
IPDINYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGE
RLWGLIVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE*"
terminator 2348..2826
/label="hGH_PA_terminator"
/translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAG
SDNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAG
FIGSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVR
KITGFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRI
IPDINYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGE
RLWGLIVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE*"
rep_origin 3098..3404
/label="f1_origin"
/translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAG
SDNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAG
FIGSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVR
KITGFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRI
IPDINYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGE
RLWGLIVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE*"
misc_feature 3672..3694
/label="pGEX_3_primer"
/translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAG
SDNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAG
FIGSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVR
KITGFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRI
IPDINYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGE
RLWGLIVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE*"
promoter 3853..3881
/label="AmpR_promoter"
/translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAG
SDNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAG
FIGSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVR
KITGFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRI
IPDINYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGE
RLWGLIVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE*"
gene 3923..4783
/label="Ampicillin"
/gene="Ampicillin"
/translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAG
SDNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAG
FIGSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVR
KITGFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRI
IPDINYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGE
RLWGLIVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE*"
CDS 3923..4783
/label="ORF frame 2"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
rep_origin 4938..5557
/label="pBR322_origin"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCGTCG GGCGACCTTT
61 GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT
121 AGGGGTTCCT GCGGCCGCAC GCGTGGAGCT AGTTATTAAT AGTAATCAAT TACGGGGTCA
181 TTAGTTCATA GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT
241 GGCTGACCGC CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA
301 ACGTCAATAG GGACTTTCCA TTGACGTCAA TGGGTGGAGT ATTTACGGTA AACTGCCCAC
361 TTGGCAGTAC ATCAAGTGTA TCATATGCCA AGTACGCCCC CTATTGACGT CAATGACGGT
421 AAATGGCCCG CCTGGCATTA TGCCCAGTAC ATGACCTTAT GGGACTTTCC TACTTGGCAG
481 TACATCTACG TATTAGTCAT CGCTATTACC ATGGTGATGC GGTTTTGGCA GTACATCAAT
541 GGGCGTGGAT AGCGGTTTGA CTCACGGGGA TTTCCAAGTC TCCACCCCAT TGACGTCAAT
601 GGGAGTTTGT TTTGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAC AACTCCGCCC
661 CATTGACGCA AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT
721 TAGTGAACCG TCAGATCGCC TGGAGACGCC ATCCACGCTG TTTTGACCTC CATAGAAGAC
781 ACCGGGACCG ATCCAGCCTC CGCGGATTCG AATCCCGGCC GGGAACGGTG CATTGGAACG
841 CGGATTCCCC GTGCCAAGAG TGACGTAAGT ACCGCCTATA GAGTCTATAG GCCCACAAAA
901 AATGCTTTCT TCTTTTAATA TACTTTTTTG TTTATCTTAT TTCTAATACT TTCCCTAATC
961 TCTTTCTTTC AGGGCAATAA TGATACAATG TATCATGCCT CTTTGCACCA TTCTAAAGAA
1021 TAACAGTGAT AATTTCTGGG TTAAGGCAAT AGCAATATTT CTGCATATAA ATATTTCTGC
1081 ATATAAATTG TAACTGATGT AAGAGGTTTC ATATTGCTAA TAGCAGCTAC AATCCAGCTA
1141 CCATTCTGCT TTTATTTTAT GGTTGGGATA AGGCTGGATT ATTCTGAGTC CAAGCTAGGC
1201 CCTTTTGCTA ATCATGTTCA TACCTCTTAT CTTCCTCCCA CAGCTCCTGG GCAACGTGCT
1261 GGTCTGTGTG CTGGCCCATC ACTTTGGCAA AGAATTGGGA TTCGAACATC GATTGAATTC
1321 CCCGGGGATC TGCCGCCACC ATGGCGGAAG GATCCGTCGC CAGGCAGCCT GACCTCTTGA
1381 CCTGCGACGA TGAGCCGATC CATATCCCCG GTGCCATCCA ACCGCATGGA CTGCTGCTCG
1441 CCCTCGCCGC CGACATGACG ATCGTTGCCG GCAGCGACAA CCTTCCCGAA CTCACCGGAC
1501 TGGCGATCGG CGCCCTGATC GGCCGCTCTG CGGCCGATGT CTTCGACTCG GAGACGCACA
1561 ACCGTCTGAC GATCGCCTTG GCCGAGCCCG GGGCGGCCGT CGGAGCACCG ATCACTGTCG
1621 GCTtCACGAT GCGAAAGgAC GCAGGCTTCA TCGGCTCCTG GCATCGCCAT GATCAGCTCA
1681 TCTtccTCGA GCTCGAGCCT CCCCAGCGGG ACGTCgccga gccgcaggcg ttcttccgcc
1741 gcaCCAACAG CGCCATCCGC CGCCTGCAGG CCGCCGAAAC CTTGGAAAGC GCCTGCGCCG
1801 CCGCGGCGCA AGAGGTGCGG AAGATTACCG GCTTCGATCG GGTGATGATC TATCGCTTCG
1861 CCTCCGACTT CAGCGGCGAA GTGATCGCAG AGGATCGGTG CGCCGAGGTC GAGTCAAAAC
1921 TAGGCCTGCA CTATCCTGCC TCAACCGTGC CGGCGCAGGC CCGTCGGCTC TATACCATCA
1981 ACCCGGTACG GATCATTCCC GATATCAATT ATCGGCCGGT GCCGGTCACC CCAGACCTCA
2041 ATCCGGTCAC CGGGCGGCCG ATTGATCTTA GCTTCGCCAT CCTGCGCAGC GTCTCGCCCG
2101 TCCATCTGGA ATTCATGCGC AACATAGGCA TGCACGGCAC GATGTCGATC TCGATTTTGC
2161 GCGGCGAGCG ACTGTGGGGA TTGATCGTTT GCCATCACCG AACGCCGTAC TACGTCGATC
2221 TCGATGGCCG CCAAGCCTGC GAGCTAGTCG CCCAGGTTCT GGCCTGGCAG ATCGGCGTGA
2281 TGGAAGAGTG AGTCGACGCG GCCGCTCGAG CCTAAGCTTG CCTCGAGCAG CGCTGCTCGA
2341 GAGATCTACG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT
2401 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC
2461 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT
2521 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA
2581 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT
2641 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG
2701 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC
2761 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT
2821 GTCCTTCTGA TTTTGTAGGT AACCACGTGC GGACCGAGCG GCCGCAGGAA CCCCTAGTGA
2881 TGGAGTTGGC CACTCCCTCT CTGCGCGCTC GCTCGCTCAC TGAGGCCGGG CGACCAAAGG
2941 TCGCCCGACG CCCGGGCTTT GCCCGGGCGG CCTCAGTGAG CGAGCGAGCG CGCAGCTGCC
3001 TGCAGGGGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA
3061 TACGTCAAAG CAACCATAGT ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT
3121 GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT
3181 CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT
3241 CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTTGGG
3301 TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA
3361 GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC
3421 GGGCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA
3481 GCTGATTTAA CAAAAATTTA ACGCGAATTT TAACAAAATA TTAACGTTTA CAATTTTATG
3541 GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGCCCC GACACCCGCC
3601 AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC
3661 TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC
3721 GAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT
3781 TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT
3841 TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA
3901 ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT
3961 TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA
4021 TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA
4081 GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT
4141 GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT
4201 ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA
4261 TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC
4321 CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT
4381 GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA
4441 CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC
4501 TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA
4561 AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC
4621 TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC
4681 CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG
4741 ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA
4801 CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA
4861 GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC
4921 GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT
4981 CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA
5041 GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT
5101 CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA
5161 CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC
5221 CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG
5281 TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG
5341 TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG
5401 CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT
5461 TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC
5521 AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT
5581 TTGCTGGCCT TTTGCTCACA TGT
//
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