| 质粒名称: | pYK672 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000838 |
| 产品用途: | Mammalian PhCMV-driven constitutive MCP-3xGNCR expression vector (PhCMV-MCP-3xGNCR-pA). |
| 存储实验室: | Anping ZENG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | MCP-3xGNCR |
| 物种来源: | synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Programmable solid-state condensates for spatiotemporal control of mammalian gene expression
Wang Y., Jiang J., Xiong Q., Li S., Shao J., Xie M., Zeng A.P.
Nat. Chem. Biol. 2025 1–10 (2025)
|
pYK672
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pYK672载体质粒基本信息
pYK672质粒图谱载体图谱和pYK672载体序列质粒序列多克隆位点信息
pYK672质粒载体简介
pYK672质粒序列载体序列
LOCUS pYK672 8041 bp DNA circular SYN 11-FEB-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8041)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 29, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8041
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 232..819
/label=CMV
primer_bind 863..882
/label=T7
primer_bind 880..899
/label=oYF0019
primer_bind 881..952
/label=oYK57-NLS-F
primer_bind 881..900
/label=oYK06-ANR-mEGFP-F
primer_bind 886..934
/label=oSCL1118
primer_bind 897..943
/label=oYK05-mcp-F
primer_bind 911..934
/label=Oscl32-1
primer_bind 911..934
/label=Oscl42-1
regulatory 918..926
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 926..1312
/codon_start=1
/product="bacteriophage MS2 coat protein"
/label=MS2-N55K
/note="binds with high affinity to a specific stem-loop
structure in the viral RNA (Lim et al., 1994)"
/translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
VKAMQGLLKDGNPIPSAIAANSGIY"
primer_bind 926..952
/label=oYK57-mcp-mCherry-F
primer_bind 926..952
/label=oYK228_mcp_F
primer_bind complement(1292..1342)
/label=oSCL093
primer_bind complement(1292..1312)
/label=Oscl32-2
primer_bind 1312..1332
/label=oLZ048 NSP3-F
misc_feature 1313..1347
/label=linker
primer_bind 1313..1342
/label=oSCL388
primer_bind 1313..1332
/label=oSCL1192
primer_bind 1313..1330
/label=oLZ147 NS3a F
primer_bind 1313..1329
/label=oYF0033
primer_bind 1313..1328
/label=oYK28-mCherry-NS3a-F
primer_bind complement(1314..1343)
/label=oLZ145 DNCR2-GNCR1 R
primer_bind complement(1323..1348)
/label=oSCL1119
primer_bind complement(1323..1342)
/label=oSCL075
primer_bind 1327..1373
/label=oLZ146 GNCR1-N F
primer_bind 1327..1343
/label=oSCL1945
primer_bind 1329..1372
/label=oYK671_GNCR_F
primer_bind complement(1330..1348)
/label=oYK460_MCPGS_R
primer_bind complement(1331..1349)
/label=oYK05-mcp-R
primer_bind complement(1331..1349)
/label=oYK82_mcp_R
primer_bind 1348..1382
/label=oYF0011
CDS 1349..2047
/codon_start=1
/label=GNCR1-N
/translation="DIEKLCKKAEEEAKEAQEKADELRQRHPDSQAAEDAEDLANLAVA
AVLTACLLAQEHPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQAA
RAVILAIMLAAENPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQA
AEAVERAIWLAAENPNADIAKKCIKAASEAAEEASKAAEEAQRHPDSQKARDEIKEASQ
KAEEVKERCKS"
primer_bind 1349..1382
/label=oYF005
primer_bind 1349..1376
/label=oYF0019
primer_bind complement(2024..2047)
/label=Primer 1
primer_bind complement(2024..2047)
/label=oYK500_GNCR_R
primer_bind complement(2026..2082)
/label=oYF0020
primer_bind complement(2026..2077)
/label=oYF006
primer_bind complement(2026..2048)
/label=oYF0012
primer_bind 2045..2067
/label=oSCL1192
primer_bind 2045..2065
/label=oLZ147 NS3a F
primer_bind 2045..2064
/label=oYF0033
primer_bind 2047..2067
/label=oLZ048 NSP3-F
primer_bind 2048..2077
/label=oSCL388
primer_bind 2048..2063
/label=oYK28-mCherry-NS3a-F
primer_bind complement(2049..2082)
/label=oLZ145 DNCR2-GNCR1 R
primer_bind complement(2058..2078)
/label=oSCL1119
primer_bind complement(2058..2077)
/label=oSCL075
primer_bind 2062..2110
/label=oSCL1945
primer_bind 2062..2088
/label=oLZ146 GNCR1-N F
misc_feature 2064..2082
/label=linker
primer_bind 2064..2078
/label=oYK671_GNCR_F
primer_bind complement(2065..2078)
/label=oYK460_MCPGS_R
primer_bind complement(2066..2078)
/label=oYK05-mcp-R
primer_bind complement(2066..2078)
/label=oYK82_mcp_R
primer_bind 2069..2109
/label=oSCL1947
CDS 2084..2782
/codon_start=1
/label=GNCR
/translation="DIEKLCKKAEEEAKEAQEKADELRQRHPDSQAAEDAEDLANLAVA
AVLTACLLAQEHPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQAA
RAVILAIMLAAENPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQA
AEAVERAIWLAAENPNADIAKKCIKAASEAAEEASKAAEEAQRHPDSQKARDEIKEASQ
KAEEVKERCKS"
primer_bind 2780..2799
/label=oYF0033
CDS 2783..2812
/codon_start=1
/label=linker
/translation="ASGSGGGGDV"
primer_bind 2786..2798
/label=oYK28-mCherry-NS3a-F
primer_bind complement(2793..2817)
/label=oSCL1946
primer_bind 2798..2844
/label=oSCL1947
misc_feature 2814..2817
/label=linker
CDS 2819..3517
/codon_start=1
/label=GNCR-2
/translation="DIEKLCKKAEEEAKEAQEKADELRQRHPDSQAAEDAEDLANLAVA
AVLTACLLAQEHPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQAA
RAVILAIMLAAENPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQA
AEAVERAIWLAAENPNADIAKKCIKAASEAAEEASKAAEEAQRHPDSQKARDEIKEASQ
KAEEVKERCKS"
primer_bind complement(3518..3559)
/label=oYK672_GNCR3_R
CDS 3518..3535
/codon_start=1
/label=linker
/translation="ASGSGG"
primer_bind complement(3518..3535)
/label=oSCL1948
primer_bind complement(3534..3561)
/label=Primer 1
primer_bind complement(3534..3561)
/label=oYK500_GNCR_R
primer_bind complement(3539..3561)
/label=oYK57-MCP-mCherry-R
terminator 3635..3862
/label=bGH PA
misc_feature 3908..4336
/label=f1 origin
misc_feature 4341..4684
/label=SV40 early promoter and origin
misc_feature 4530..4607
/label=SV40 ORI
CDS 4749..5540
/label=Kan/neoR
misc_feature 5714..5844
/label=SV40 early polyadenylation signal
primer_bind complement(5880..5900)
/label=M13-rev
misc_binding complement(5906..5928)
/label=LacO
promoter complement(5933..5962)
/label=lac
misc_feature complement(6227..6900)
/label=pUC origin
CDS complement(7048..7707)
/label=AmpR
promoter complement(7947..7975)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagcttgcca ccatggcttc aaactttact cagttcgtgc tcgtggacaa
961 tggtgggaca ggggatgtga cagtggctcc ttctaatttc gctaatgggg tggcagagtg
1021 gatcagctcc aactcacgga gccaggccta caaggtgaca tgcagcgtca ggcagtctag
1081 tgcccagaag agaaagtata ccatcaaggt ggaggtcccc aaagtggcta cccagacagt
1141 gggcggagtc gaactgcctg tcgccgcttg gaggtcctac ctgaacatgg agctcactat
1201 cccaattttc gctaccaatt ctgactgtga actcatcgtg aaggcaatgc aggggctcct
1261 caaagacggt aatcctatcc cttccgccat cgccgctaac tcaggtatct acgccagcgg
1321 aagtggcgga ggaggcgacg tcggatccga catcgagaag ctgtgcaaga aggcggaaga
1381 ggaggccaag gaggcccagg agaaggccga cgagctgaga caacgacacc ccgattcgca
1441 ggcggcggaa gacgcggagg atctcgcaaa tctggctgtc gccgccgtgc tgaccgcttg
1501 tctgctggcc caggagcatc ccaacgccga tatcgctaag ctgtgcatca aggccgcgag
1561 cgaagctgcg gaggccgcgt caaaagccgc cgagctggcc caacggcacc cagacagtca
1621 agcagccaga gatgctatca aactcgcctc tcaagccgcc cgcgccgtga tcctggccat
1681 catgctcgcc gcagagaatc ccaacgctga tattgcaaag ctatgtatta aggcggccag
1741 cgaagccgca gaggctgcct cgaaagctgc ggaacttgca cagcggcacc ccgactccca
1801 ggccgcccga gatgcaatta aactggccag tcaggccgct gaggcggtgg agagagccat
1861 ctggctggcc gccgagaacc ccaatgccga tatcgccaaa aaatgcatca aagcggcctc
1921 cgaagcagcc gaggaggcct ctaaagctgc cgaagaggct caaagacacc cagacagcca
1981 gaaggcacgc gacgagatca aggaggcctc tcagaaggcc gaggaggtga aggagagatg
2041 caagagcgcc agcggaagtg gcggaggagg cgacgtcgct agtgacattg agaagctctg
2101 taagaaagcc gaagaagagg ctaaagaagc acaggagaaa gctgatgagt tgcggcagcg
2161 gcacccggat tcacaagcgg ccgaagacgc agaggacctg gccaacctgg ccgtagccgc
2221 ggttctgacc gcatgtctgc tcgcccaaga gcatcctaac gcagatatcg ctaagctgtg
2281 catcaaggca gcgtccgagg ccgccgaggc cgcaagtaaa gccgctgaat tagcacaaag
2341 gcatccagac agtcaggccg caagggacgc aatcaaactg gcatctcagg ctgccagagc
2401 ggtgatcctt gcaattatgc tggccgccga aaacccaaat gctgatatag caaaactgtg
2461 catcaaagca gccagcgaag cagcggaagc cgcttccaag gccgctgagt tggcccaaag
2521 acatccagat agccaagcag cccgtgatgc gatcaaactc gctagtcagg ctgccgaggc
2581 cgttgaacgt gctatatggc tggcggccga aaatccaaat gccgacatag ccaaaaagtg
2641 tatcaaggcc gcctcagaag ctgccgaaga ggcgagcaag gccgcagaag aggcccagcg
2701 ccatcccgat tcccagaagg cccgggatga gatcaaagaa gccagccaga aagctgagga
2761 ggtcaaagag cgatgtaaga gtgcgagcgg aagtggcggt ggcggtgacg tggctagtga
2821 tatcgagaag ttgtgtaaaa aagctgagga agaggcaaaa gaggcccagg aaaaggcaga
2881 cgagcttcgt cagagacatc ctgattccca ggccgcggag gatgctgagg acctcgccaa
2941 tttggccgtc gcagctgtgc tgacggcctg cctccttgcg caagaacacc ctaatgcgga
3001 catagctaaa ctctgcatta aggccgcctc agaggccgca gaagccgctt caaaagctgc
3061 cgaactagca cagagacatc ctgactctca ggcagctaga gacgctatta agcttgcttc
3121 tcaagccgcc cgagccgtta tcctggccat tatgctagcc gcagagaatc cgaacgccga
3181 catcgcaaaa ctgtgcataa aggccgcatc tgaggcagca gaggcagcca gtaaggccgc
3241 tgaactcgca cagaggcacc ctgattcaca ggcagcgcgg gatgctatta agttagcttc
3301 acaggctgcc gaagctgtgg agagagccat ttggcttgct gcggaaaatc caaatgccga
3361 catcgccaag aaatgcatca aagctgcctc cgaggcggcc gaagaagcca gtaaagctgc
3421 agaggaagct cagcgccacc ctgactctca gaaagcgcgg gacgagatta aagaggcaag
3481 ccagaaagca gaagaggtca aagagcgctg taagagcgct agtggctccg gaggctaagg
3541 atccactagt ccagtgtggt ggaattctgc agatatccag cacagtggcg gccgctcgag
3601 tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
3661 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
3721 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
3781 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
3841 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
3901 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
3961 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
4021 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
4081 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
4141 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
4201 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
4261 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
4321 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
4381 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
4441 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
4501 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
4561 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
4621 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
4681 agctcccggg agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt
4741 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
4801 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
4861 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
4921 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
4981 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
5041 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
5101 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
5161 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
5221 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
5281 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
5341 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
5401 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
5461 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
5521 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
5581 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
5641 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
5701 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
5761 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
5821 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
5881 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
5941 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
6001 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
6061 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
6121 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
6181 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
6241 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
6301 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
6361 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
6421 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
6481 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
6541 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
6601 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
6661 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
6721 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
6781 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
6841 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
6901 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
6961 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
7021 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
7081 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
7141 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
7201 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
7261 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
7321 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
7381 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
7441 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
7501 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
7561 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
7621 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
7681 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
7741 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
7801 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
7861 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
7921 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
7981 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
8041 c
//
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