| 质粒名称: | pLVCT-tTR-KRAB |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 11643 |
| 存储实验室: | Patrick Aebischer, Didier Trono |
|
载体骨架基本信息 |
|
| 载体名称: | -- |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 12877 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | CAG promoters, GFP, tTR-KRAB, Tet-on |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | See map |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pLVCT-tTR-KRAB was a gift from Patrick Aebischer & Didier Trono (Addgene plasmid # 11643 ; http://n2t.net/addgene:11643 ; RRID:Addgene_11643) |
| 参考文献部分: | A versatile tool for conditional gene expression and knockdown. Szulc J, Wiznerowicz M, Sauvain MO, Trono D, Aebischer P. Nat Methods. 2006 Feb . 3(2):109-16. 10.1038/nmeth846 PubMed 16432520 |
买家导航
pLVCT-tTR-KRAB载体质粒基本信息
pLVCT-tTR-KRAB质粒图谱载体图谱和pLVCT-tTR-KRAB载体序列质粒序列多克隆位点信息
pLVCT-tTR-KRAB质粒载体简介
pLVCT-tTR-KRAB质粒序列载体序列
LOCUS pLVCT-tTR-KRAB 12884 bp ds-DNA circular SYN 23-MAR-2020
DEFINITION Tet-regulated (Tet-on) lentiviral vector for transgene (CAG
promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM
(Addgene#12247)) - 2nd generation.
ACCESSION .
VERSION .
KEYWORDS pLVCT-tTR-KRAB
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12884)
AUTHORS Szulc J, Wiznerowicz M, Sauvain MO, Trono D, Aebischer P
TITLE A versatile tool for conditional gene expression and knockdown.
JOURNAL Nat Methods. 2006 Feb . 3(2):109-16.
PUBMED 16432520
REFERENCE 2 (bases 1 to 12884)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Mar 23, 2020 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..12884
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 6..639
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 686..811
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1304..1537
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
enhancer 2061..2440
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2442..2719
/label=chicken beta-actin promoter
intron 2720..3736
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
primer_bind 3660..3682
/label=pCAGGS-5
/note="Chimeric intron in CAG promoter, forward primer"
primer_bind 3744..3763
/label=pCAG-F
/note="Rabbit beta-globin intron, for pCAG plasmids,
forward primer"
misc_feature 3822..3939
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
regulatory 4016..4025
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 4022..4738
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(4067..4088)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(4328..4347)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 4675..4696
/label=EGFP-C
/note="EGFP, forward primer"
misc_feature 4834..5385
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(4978..4995)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 5205..5224
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 5388..6005
/codon_start=1
/gene="tetR from transposon Tn10"
/product="tetracycline repressor TetR"
/label=TetR
/note="TetR binds to the tetracycline operator tetO to
inhibit transcription. This inhibition can be relieved by
adding tetracycline or doxycycline."
/translation="MARLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESG"
primer_bind complement(5461..5480)
/label=Tet-R
/note="Tetracycline resistance gene, reverse primer"
CDS 6009..6029
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 6132..6326
/codon_start=1
/gene="ZNF10"
/gene_synonym="KOX1"
/product="Kruppel-associated box (KRAB) transcriptional
repression domain from the human zinc finger protein ZNF10
(Margolin et al., 1994)"
/label=KRAB
/translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
QLTKPDVILRLEKGEEPWLV"
misc_feature 6469..7057
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(6522..6542)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(6940..6951)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
protein_bind 7195..7465
/label=tetracycline response element
/bound_moiety="tetracycline repressor TetR"
/note="contains seven copies of the tetracycline operator
tetO"
LTR 7504..7684
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
promoter complement(7709..7727)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(7710..7727)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(8017..8036)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
primer_bind complement(8250..8269)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 8369..8391
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(8429..8447)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 8515..8619
/gene="bla"
/label=AmpR promoter
CDS 8620..9480
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(8838..8857)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 9651..10239
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 10140..10159
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 10393..10410
/label=L4440
/note="L4440 vector, forward primer"
primer_bind complement(10480..10500)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 10485..10814
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 10665..10800
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 10727..10746
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
intron 11948..12013
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 12143..12163
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 12588..12722
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(12625..12644)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 12679..12698
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
ORIGIN
1 gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc tgtggatcta
61 ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata
121 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga
181 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga
241 cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc
301 ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact
361 ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct
421 cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga
481 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
541 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
601 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
661 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
721 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
781 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
841 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
901 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
961 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
1021 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
1081 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
1141 acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
1201 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
1261 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
1321 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
1381 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
1441 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
1501 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
1561 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
1621 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
1681 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
1741 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
1801 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
1861 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
1921 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
1981 acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgatca cgagactagc
2041 ctcgaccatc gatgtcgatc gacattgatt attgactagt tattaatagt aatcaattac
2101 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
2161 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
2221 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
2281 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
2341 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
2401 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt
2461 ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt
2521 ttaattattt tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc naggsggggc
2581 ggggcggggc gaggggcggg gcggggcgag gcggagaggt gcggcggcag ccaatcagag
2641 cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa
2701 gcgaagcgcg cggcgggcgg gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc
2761 cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg
2821 gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc
2881 ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag
2941 cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct
3001 gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg
3061 cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa
3121 aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg
3181 gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc
3241 ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt
3301 gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aggggcgcgg
3361 cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt gccttttatg
3421 gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa atctgtgcgg agccgaaatc
3481 tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag
3541 gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct
3601 ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg gggggacggg gcagggcggg
3661 gttcggcttc tggcgtgtga ccggcggctc tagagcctct gctaaccatg ttcatgcctt
3721 cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg
3781 caaagaatta aatttaatta atctcgacgg tatcggttaa cttttaaaag aaaagggggg
3841 attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact
3901 aaagaattac aaaaacaaat tacaaaaatt caaaatttta tcgatcacga gactagcctc
3961 gaggtttaaa ctacgggatc caggcctaag cttacgcgtc ctagcgctac cggtcgccac
4021 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
4081 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
4141 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
4201 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
4261 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
4321 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
4381 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
4441 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
4501 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
4561 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
4621 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
4681 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagtc
4741 cggactcaga tctcgactag ctagtagcta gctagctagt cgagctcaag cttcgaattc
4801 ccgggctgca ggaattccgc cccccccccc ctaacgttac tggccgaagc cgcttggaat
4861 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
4921 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
4981 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
5041 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
5101 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
5161 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
5221 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
5281 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaacgtc taggcccccc
5341 gaaccacggg gacgtggttt tcctttgaaa aacacgatga taataccatg gctagattag
5401 ataaaagtaa agtgattaac agcgcattag agctgcttaa tgaggtcgga atcgaaggtt
5461 taacaacccg taaactcgcc cagaagctag gtgtagagca gcctacattg tattggcatg
5521 taaaaaataa gcgggctttg ctcgacgcct tagccattga gatgttagat aggcaccata
5581 ctcacttttg ccctttagaa ggggaaagct ggcaagattt tttacgtaat aacgctaaaa
5641 gttttagatg tgctttacta agtcatcgcg atggagcaaa agtacattta ggtacacggc
5701 ctacagaaaa acagtatgaa actctcgaaa atcaattagc ctttttatgc caacaaggtt
5761 tttcactaga gaatgcatta tatgcactca gcgctgtggg gcattttact ttaggttgcg
5821 tattggaaga tcaagagcat caagtcgcta aagaagaaag ggaaacacct actactgata
5881 gtatgccgcc attattacga caagctatcg aattatttga tcaccaaggt gcagagccag
5941 ccttcttatt cggccttgaa ttgatcatat gcggattaga aaaacaactt aaatgtgaaa
6001 gtgggtcgcc aaaaaagaag agaaaggtcg acggcggtgg tgctttgtct cctcagcact
6061 ctgctgtcac tcaaggaagt atcatcaaga acaaggaggg catggatgct aagtcactaa
6121 ctgcctggtc ccggacactg gtgaccttca aggatgtatt tgtggacttc accagggagg
6181 agtggaagct gctggacact gctcagcaga tcgtgtacag aaatgtgatg ctggagaact
6241 ataagaacct ggtttccttg ggttatcagc ttactaagcc agatgtgatc ctccggttgg
6301 agaagggaga agagccctgg ctggtggaga gagaaattca ccaagagacc catcctgatt
6361 cagagactgc atttgaaatc aaatcatcag tttaaggatc tcgactagct agtagctagc
6421 tagctagtcg agctcaagct tcgaattcga tgggactagt catatgataa tcaacctctg
6481 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
6541 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
6601 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
6661 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
6721 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
6781 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
6841 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
6901 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
6961 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
7021 cagacgagtc ggatctccct ttgggccgcc tccccgcatc ggtacgtatg gccaggtacc
7081 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
7141 gggactggaa gggctaattc actcccaacg aagacaagat ctcgagttta ccactcccta
7201 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
7261 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
7321 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
7381 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
7441 taccactccc tatcagtgat agagaaaagt gaaagtcgga tcgatctgct ttttgcttgt
7501 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
7561 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
7621 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
7681 agcagcatct agaattaatt ccgtgtattc tatagtgtca cctaaatcgt atgtgtatga
7741 tacataaggt tatgtattaa ttgtagccgc gttctaacga caatatgtac aagcctaatt
7801 gtgtagcatc tggcttactg aagcagaccc tatcatctct ctcgtaaact gccgtcagag
7861 tcggtttggt tggacgaacc ttctgagttt ctggtaacgc cgtcccgcac ccggaaatgg
7921 tcagcgaacc aatcagcagg gtcatcgcta gccagatcct ctacgccgga cgcatcgtgg
7981 ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg
8041 gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg
8101 caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg
8161 cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata
8221 agggagagcg tcgaatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
8281 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
8341 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
8401 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
8461 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
8521 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
8581 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
8641 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
8701 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
8761 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
8821 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
8881 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
8941 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
9001 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
9061 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
9121 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
9181 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
9241 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
9301 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
9361 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
9421 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
9481 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
9541 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
9601 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
9661 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
9721 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
9781 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
9841 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
9901 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
9961 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
10021 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
10081 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
10141 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
10201 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
10261 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
10321 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
10381 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
10441 cctctccccg cgcgttggcc gattcattaa tgcagctgtg gaatgtgtgt cagttagggt
10501 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
10561 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
10621 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
10681 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
10741 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
10801 taggcttttg caaaaagctt ggacacaaga caggcttgcg agatatgttt gagaatacca
10861 ctttatcccg cgtcagggag aggcagtgcg taaaaagacg cggactcatg tgaaatactg
10921 gtttttagtg cgccagatct ctataatctc gcgcaaccta ttttcccctc gaacactttt
10981 taagccgtag ataaacaggc tgggacactt cacatgagcg aaaaatacat cgtcacctgg
11041 gacatgttgc agatccatgc acgtaaactc gcaagccgac tgatgccttc tgaacaatgg
11101 aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg tgcgttactg gcgcgtgaac
11161 tgggtattcg tcatgtcgat accgtttgta tttccagcta cgatcacgac aaccagcgcg
11221 agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt attgatgacc
11281 tggtggatac cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa gcgcactttg
11341 tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt gttgatatcc
11401 cgcaagatac ctggattgaa cagccgtggg atatgggcgt cgtattcgtc ccgccaatct
11461 ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt tgttcttttt aacttcaggc
11521 gggttacaat agtttccagt aagtattctg gaggctgcat ccatgacaca ggcaaacctg
11581 agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct agtccgccgc
11641 tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg atggtaaaac catccctcac
11701 tggtatcgca tgattaaccg tctgatgtgg atctggcgcg gcattgaccc acgcgaaatc
11761 ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac gtaccgacga tgatttatac
11821 gatacggtga ttggctaccg tggcggcaac tggatttatg agtgggcccc ggatctttgt
11881 gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag
11941 ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt
12001 ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt
12061 taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc
12121 tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt
12181 tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg
12241 ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa
12301 atattctgta acctttataa gtaggcataa cagttataat cataacatac tgttttttct
12361 tactccacac aggcatagag tgtctgctat taataactat gctcaaaaat tgtgtacctt
12421 tagcttttta atttgtaaag gggttaataa ggaatatttg atgtatagtg ccttgactag
12481 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
12541 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
12601 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
12661 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
12721 tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta
12781 ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt
12841 cattttaaag aaattgtatt tgttaaatat gtactacaaa ctta
//
