pAAVLINKv1-pCALM1-Cre-3'-NRXN3-FLAG

LOCUS       pAAVLINKv1-pCALM1-Cre-3'-NRXN3-FLAG              7179 bp DNA     circular SYN 10-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7179)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 9, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7179
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     promoter        145..265
                     /label=pCALM1
     CDS             277..1308
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     intron          1309..1349
                     /label=Splicing Donor
     protein_bind    1356..1389
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1390..1481
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1482..3892
                     /codon_start=1
                     /gene="NRXN3"
                     /gene_synonym="C14orf60"
                     /product="neurexin 3 isoform 5"
                     /label=NRXN3
                     /note="isoform 5 is encoded by transcript variant 
                     7;neurexin III; neurexin-3-alpha"
                     /db_xref="GI:1056849789"
                     /db_xref="CCDS:CCDS81831.1"
                     /db_xref="GeneID:9369"
                     /db_xref="HGNC:HGNC:8010"
                     /db_xref="MIM:600567"
                     /protein_id="NP_001317124.1"
                     /translation="QKLNDNEWHTVRVVRRGKSLKLTVDDDVAEGTMVGDHTRLEFHNI
                     ETGIMTEKRYISVVPSSFIGHLQSLMFNGLLYIDLCKNGDIDYCELKARFGLRNIIADP
                     VTFKTKSSYLSLATLQAYTSMHLFFQFKTTSPDGFILFNSGDGNDFIAVELVKGYIHYV
                     FDLGNGPNVIKGNSDRPLNDNQWHNVVITRDNSNTHSLKVDTKVVTQVINGAKNLDLKG
                     DLYMAGLAQGMYSNLPKLVASRDGFQGCLASVDLNGRLPDLINDALHRSGQIERGCEGP
                     STTCQEDSCANQGVCMQQWEGFTCDCSMTSYSGNQCNDPGATYIFGKSGGLILYTWPAN
                     DRPSTRSDRLAVGFSTTVKDGILVRIDSAPGLGDFLQLHIEQGKIGVVFNIGTVDISIK
                     EERTPVNDGKYHVVRFTRNGGNATLQVDNWPVNEHYPTGNTDNERFQMVKQKIPFKYNR
                     PVEEWLQEKGRQLTIFNTQAQIAIGGKDKGRLFQGQLSGLYYDGLKVLNMAAENNPNIK
                     INGSVRLVGEVPSILGTTQTTSMPPEMSTTVMETTTTMATTTTRKNRSTASIQPTSDDL
                     VSSAECSSDDEDFVECEPSTDKSLSTSIFEGGYKAHAPKWESKDFRPNKVSETSRTTTT
                     SLSPELIRFTASSSSGMVPKLPAGKMNNRDLKPQPDIVLLPLPTAYELDSTKLKSPLIT
                     SPMFRNVPTANPTEPGIRRVPGASEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYR
                     NRDEGSYQVDETRNYISNSAQSNGTLMKEKQQSSKSGHKKQKNKDREYYV"
     misc_feature    3423..3428
                     /label=SDS
     misc_feature    3715..3720
                     /label=SDS
     CDS             3899..3964
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3968..4399
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    4112..4117
                     /label=SDS
     repeat_region   4442..4582
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4442..4571
                     /label=AAV2 ITR
     repeat_region   4443..4583
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4657..5112
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5394..5498
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5499..6359
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6530..7118
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
      181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
      241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
      301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
      361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
      421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
      481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
      541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
      601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
      661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
      721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
      781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
      841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
      901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
      961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
     1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
     1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
     1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
     1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
     1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
     1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
     1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
     1441 tattggtctt actgacatcc actttgcctt tctctccaca gggcagaagc tcaatgacaa
     1501 cgagtggcac accgttcggg tggtgcggag aggaaaaagc cttaagttaa ccgtggatga
     1561 tgatgtggct gagggtacaa tggtgggaga ccatacccgt ttggagttcc acaacattga
     1621 aacgggaatc atgactgaga aacgctacat ctccgttgtc ccctccagct ttattggcca
     1681 tctgcagagc ctcatgttta atggccttct ctacattgac ttgtgcaaaa atggtgacat
     1741 tgattattgt gagctgaagg ctcgttttgg actgaggaac atcatcgctg accctgtcac
     1801 ctttaagacc aagagcagct acctgagcct tgccactctt caggcttaca cctccatgca
     1861 cctcttcttc cagttcaaga ccacctcacc agatggcttc attctcttca atagtggtga
     1921 tggcaatgac ttcattgcag tcgagcttgt caaggggtat atacactacg tttttgacct
     1981 cggaaacggt cccaatgtga tcaaaggcaa cagtgaccgc cccctgaatg acaaccagtg
     2041 gcacaatgtc gtcatcactc gggacaatag taacactcat agcctgaaag tggacaccaa
     2101 agtggtcact caggttatca atggtgccaa aaatctggat ttgaaaggtg atctctatat
     2161 ggctggtctg gcccaaggca tgtacagcaa cctcccaaag ctcgtggcct ctcgagatgg
     2221 ctttcagggc tgtctagcat cagtggactt gaatggacgc ctgccagacc tcatcaatga
     2281 tgctcttcat cggagcggac agatcgagcg tggctgtgaa ggacccagta ccacctgcca
     2341 ggaagattca tgtgccaacc agggggtctg catgcaacaa tgggagggct tcacctgtga
     2401 ttgttctatg acctcttatt ctggaaacca gtgcaatgat cctggcgcta cgtacatctt
     2461 tgggaaaagt ggtgggctta tcctctacac ctggccagcc aatgacaggc ccagcacgcg
     2521 gtctgaccgc cttgccgtgg gcttcagcac cactgtgaag gatggcatct tggtccgcat
     2581 cgacagtgct ccaggacttg gtgacttcct ccagcttcac atagaacagg ggaaaattgg
     2641 agttgtcttc aacattggca cagttgacat ctccatcaaa gaggagagaa cccctgtaaa
     2701 tgacggcaaa taccatgtgg tacgcttcac caggaacggc ggcaacgcca ccctgcaggt
     2761 ggacaactgg ccagtgaatg aacattatcc tacaggcaac actgataatg aacgcttcca
     2821 aatggtaaaa cagaaaatcc ccttcaaata taatcggcct gtagaggagt ggctgcagga
     2881 aaaaggccgg cagttaacca tcttcaacac tcaggcgcaa atagccattg gtggaaagga
     2941 caaaggacgc ctcttccaag gccaactctc tgggctctat tatgatggtt tgaaagtact
     3001 gaacatggcg gctgagaaca accccaatat taaaatcaat ggaagtgttc ggctggttgg
     3061 agaagtccca tcaattttgg gaacaacaca gacgacctcc atgccaccag aaatgtctac
     3121 tactgtcatg gaaaccacta ctacaatggc gactaccaca acccgtaaga atcgctctac
     3181 agccagcatt cagccaacat cagatgatct tgtttcatct gctgaatgtt caagtgatga
     3241 tgaagacttt gttgaatgtg agccgagtac agataagagt ctttccactt caatcttcga
     3301 aggtggctac aaagcacatg cgcccaagtg ggaatccaag gactttagac ctaacaaagt
     3361 ctccgaaact agtaggacta ctaccacatc tttatcccct gagctgatcc gcttcacagc
     3421 ttcctcctcg tctgggatgg tgcccaaatt gccagctggc aaaatgaata accgtgatct
     3481 caaaccccag cctgatatag tcttgcttcc gttgcccact gcctatgagc tagacagcac
     3541 caaactgaag agcccactaa ttacttcccc catgttccgt aatgtgccca cagcaaaccc
     3601 cacggagccg ggaatcagac gggttccggg ggcctcagag gtgatccggg agtcgagcag
     3661 cacaacaggg atggtcgtcg gcattgtggc tgctgccgcc ctctgcatct tgatcctcct
     3721 gtacgccatg tacaagtaca ggaacaggga cgaggggtcc tatcaagtgg acgagacgcg
     3781 gaactacatc agcaactccg cccagagcaa cggcacgctc atgaaggaga agcagcagag
     3841 ctcgaagagc ggccacaaga aacagaaaaa caaggacagg gagtattacg tgtgtacaga
     3901 ctacaaagac catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga
     3961 caagtaactc gagataatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     4021 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     4081 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttagttctt
     4141 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     4201 ggcactgaca attccgtggt gtttatttgt gaaatttgtg atgctattgc tttatttgta
     4261 accatctagc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     4321 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
     4381 gatgtgggag gttttttaaa gatctggaga ccggtgtggc acgtgcggac cgagcggccg
     4441 caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag
     4501 gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag
     4561 cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc
     4621 ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa
     4681 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccttagcgc
     4741 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     4801 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     4861 aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     4921 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     4981 cactcaactc tatctcgggc tattcttttg atttataagg gattttgccg atttcggtct
     5041 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     5101 cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     5161 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     5221 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     5281 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     5341 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     5401 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     5461 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     5521 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     5581 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     5641 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     5701 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     5761 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     5821 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     5881 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     5941 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     6001 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     6061 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     6121 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     6181 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     6241 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     6301 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     6361 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     6421 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     6481 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6541 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6601 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6661 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     6721 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     6781 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     6841 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     6901 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     6961 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     7021 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     7081 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     7141 tacggttcct ggccttttgc tggccttttg ctcacatgt
//