pAAV-Syn-SomArchon

LOCUS      pAAV-Syn-SomArchon        6522 bp DNA     circular SYN 25-AUG-2024
DEFINITION  Expression of SomArchon under Syn promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6522)
  AUTHORS   Piatkevich KD, Bensussen S, Tseng HA, Shroff SN, Lopez-Huerta VG, 
            Park D, Jung EE, Shemesh OA, Straub C, Gritton HJ, Romano MF, Costa 
            E, Sabatini BL, Fu Z, Boyden ES, Han X
  TITLE     Population imaging of neural activity in awake behaving mice.
  JOURNAL   Nature. 2019 Oct;574(7778):413-417. doi: 10.1038/s41586-019-1641-1. 
            Epub 2019 Oct 9.
  PUBMED    31597963
REFERENCE   2  (bases 1 to 6522)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 25, 2024 from SnapGene Server 7.0.3
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-019-1641-1"; journalName: "Nature"; date: "2019-10";
            volume: "574"; issue: "7778"; pages: "413-417"
FEATURES             Location/Qualifiers
     source          1..6522
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        341..788
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      801..810
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1674..2387
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             1674..2387
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1716..1737)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1977..1996)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2324..2345
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2655..3243
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2708..2728)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3126..3137)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3263..3739
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3462..3481)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3785..3925
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3785..3914
                     /label=AAV2 ITR
     rep_origin      4000..4455
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4087..4106)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4297..4318
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4472..4491)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4591..4613
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4651..4669)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4737..4841
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4842..5702
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5060..5079)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5873..6461
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6362..6381
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgttgcaaa gatggataaa gttttaaaca gagaggaatc
      181 tttgcagcta atggaccttc taggtcttga aaggagtggg aattggctcc ggtgcccgtc
      241 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcagca
      301 aatggttaat taatctagac tgcagagggc cctgcgtatg agtgcaagtg ggttttagga
      361 ccaggatgag gcggggtggg ggtgcctacc tgacgaccga ccccgaccca ctggacaagc
      421 acccaacccc cattccccaa attgcgcatc ccctatcaga gagggggagg ggaaacagga
      481 tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg gacagtgcct tcgcccccgc
      541 ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc gctgacgtca ctcgccggtc
      601 ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg cgccgccgcc ggcccagccg
      661 gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg cgaccatctg cgctgcggcg
      721 ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg aggagtcgtg tcgtgcctga
      781 gagcgcagtc gagaggatcc gccaccatgg tttctatcgc actgcaggct ggttatgacc
      841 tcctcggaga tggtagacct gaaagtctgt ggctcggaat tggtacactg ctgatgctga
      901 tcggaacctt ctacttcctg gttagagcgt ggggagagac tgacaaggat gcacgggagt
      961 actacgcagt gacaatcctg gtcagcggta ttgccagtgc agcttatctg tcaatgttct
     1021 ttggtattgg cctcacagag gtgccagtag gtggagagat gctgaacatc tactacgcaa
     1081 gatacgcaca gtggctgttc actacaccac tgttgctgct gcacctggca ctcctggcca
     1141 aagttgatag agtgaccatc ggcactctcg ttggcgtcga tgctctgatg attgtcacag
     1201 gcctgatagg aacactgtca catactgcca ttgccagata ctcttggtgg ctgttctcaa
     1261 ctatctgtat gatcgtcgtt ctgtatgtcc tggccacttc tctgcggtca gccgctaagg
     1321 agagaggccc agaggttgcc tcaaccttca acatcctcac cgcactggtt ctggtcctgt
     1381 ggactgctta ccctatcatc tggattatcg gcaccgaggg tgccggagtt gtgggcctgg
     1441 gaatcgaaac tttgctgttc atggtgctgg acgtcaccgc caaggtgggc ttcggtttta
     1501 tcctgctcag gtcaagagcc attctgcaag atacagaagc acctgaacct tcagctggag
     1561 ctgataccgg tgccgccgac cgcccggtag tagcagtgag caaggcggcc gccaagagca
     1621 ggatcaccag cgagggcgag tacatccccc tggaccagat cgacatcaac gtggtgagca
     1681 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     1741 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     1801 ccctgaagtt catttgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     1861 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     1921 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     1981 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     2041 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     2101 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     2161 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     2221 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     2281 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     2341 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagggt gggagcggag
     2401 gtacccagag ccagcctatc ctgaacacca aggagatggc ccctcagagc aagcctcctg
     2461 aggagctgga gatgagcagc atgcctagcc ctgtggcccc tctgcctgcc aggaccgagg
     2521 gcgtgatcga catgaggagc atgagcagca tcgacagctt catcagctgc gccaccgact
     2581 tccctgaggc caccaggttc tacaagttct gctacgagaa cgaggtgtaa gaattcgata
     2641 tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     2701 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     2761 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     2821 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     2881 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     2941 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     3001 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     3061 ttccttggct gctcgcctat gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     3121 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     3181 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     3241 cgcatcgata ccggctcgag acgggtggca tccctgtgac ccctccccag tgcctctcct
     3301 ggccctggaa gttgccactc cagtgcccac cagccttgtc ctaataaaat taagttgcat
     3361 cattttgtct gactaggtgt ccttctataa tattatgggg tggagggggg tggtatggag
     3421 caaggggcaa gttgggaaga caacctgtag ggcctgcggg gtctattggg aaccaagctg
     3481 gagtgcagtg gcacaatctt ggctcactgc aatctccgcc tcctgggttc aagcgattct
     3541 cctgcctcag cctcccgagt tgttgggatt ccaggcatgc atgaccaggc tcagctaatt
     3601 tttgtttttt tggtagagac ggggtttcac catattggcc aggctggtct ccaactccta
     3661 atctcaggtg atctacccac cttggcctcc caaattgctg ggattacagg cgtgaaccac
     3721 tgctcccttc cctgtccttc tcgagctgat tttgtaggta accacgtgcg gaccgagcgg
     3781 ccgcaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact
     3841 gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc
     3901 gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt attttctcct tacgcatctg
     3961 tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat
     4021 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccttag
     4081 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     4141 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     4201 ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     4261 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     4321 caacactcaa ctctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg
     4381 tctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     4441 taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     4501 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     4561 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     4621 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
     4681 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     4741 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     4801 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     4861 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     4921 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     4981 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     5041 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     5101 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     5161 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     5221 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     5281 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     5341 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     5401 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     5461 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     5521 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     5581 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     5641 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     5701 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     5761 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     5821 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     5881 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     5941 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     6001 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     6061 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     6121 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     6181 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     6241 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     6301 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     6361 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     6421 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     6481 ttttacggtt cctggccttt tgctggcctt ttgctcacat gt
//