| 质粒名称: | pAAV-CAG-LSSmScarlet2-P2A-EGFP |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000166 |
| 产品用途: | Expresses the LSSmScarlet2 chemically stable red fluorescent protein with a large Stock's shift in mammalian cells |
| 存储实验室: | Laboratory of Molecular Design |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 腺病毒，哺乳动物表达 |
| 启动子: | CAG |
| 拷贝数: | 低拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Mach1 |
|
插入基因信息 |
|
| 插入基因名称: | LSSmScarlet2-P2A-EGFP |
| 物种来源: | Synthetic |
| 密码子优化: | 细菌表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
LSSmScarlet2 and LSSmScarlet3, Chemically Stable Genetically Encoded Red Fluorescent Proteins with a Large Stokes' Shift
Subach O.M., Vlaskina A.V., Agapova Y.K., Piatkevich K.D., Patrushev M.V., Samygina V.R., Subach F.V.
Int. J. Mol. Sci. 23, 11051 (2022)
|
买家导航
pAAV-CAG-LSSmScarlet2-P2A-EGFP载体质粒基本信息
pAAV-CAG-LSSmScarlet2-P2A-EGFP质粒图谱载体图谱和pAAV-CAG-LSSmScarlet2-P2A-EGFP载体序列质粒序列多克隆位点信息
pAAV-CAG-LSSmScarlet2-P2A-EGFP质粒载体简介
pAAV-CAG-LSSmScarlet2-P2A-EGFP质粒序列载体序列
LOCUS pAAV-CAG-LSSmScarlet2-P2A-EGFP 6277 bp DNA circular SYN 22-APR-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6277)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 28, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6277
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin complement(182..770)
/direction=LEFT
/label=ori
/note="high-copy-number colE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(941..1801)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(1802..1906)
/gene="bla"
/label=AmpR promoter
rep_origin complement(1932..2387)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2942..3219
/label=chicken beta-actin promoter
regulatory 3643..3652
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 3649..4344
/codon_start=1
/label=LSSmScarlet2
/translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYHKQSFPEGFKWERVMNFEDGG
AVTVTQDTSLEDGTLIYEVKLRGTNFPPDGPVMQKKTMGLEADTERLYPEDGVLKGDIK
MALRLKGGGRYLAHVRTTYKAKKPVLMPGAYNVDRKLDITSHNEDYTVVEQFERSEGRH
STGDMDELYK"
CDS 4369..4425
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 4438..5154
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITLGMDELYK"
misc_feature 5179..5767
/label=WPRE
/note="woodchuck posttranscriptional regulatory element"
CDS complement(5650..5661)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 5810..5931
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
ORIGIN
1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
1921 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
1981 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
2041 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
2101 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
2161 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
2221 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
2281 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
2341 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt
2401 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
2461 gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg cgacctttgg
2521 tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact ccatcactag
2581 gggttccttg tagttaatga ttaacccgcc atgctactta tctacgtagc catgctctag
2641 gaagatcgta ggttaattaa ggcgcgcccc taggggagat ggccagccag cccattgacg
2701 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
2761 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
2821 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
2881 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
2941 gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca
3001 attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg
3061 gggcgcgcgc caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt
3121 gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
3181 cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gacgctgcct
3241 tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc
3301 gttactccca caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt
3361 ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa agccttgagg ggctccggga
3421 gggccctttg tgcgggggga gcggctcggg gctgtccgcg gggggacggc tgccttcggg
3481 ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg
3541 ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg
3601 tgctgtctca tcattttggc aaagaattgg ggatccggta ccgccaccat ggtgagcaag
3661 ggcgaggcag tgatcaagga gttcatgcgg ttcaaggtgc acatggaggg ctccatgaac
3721 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
3781 gccaagctga aggtgaccaa gggtggtcca ctgcccttct cctgggacat cctgtcccct
3841 cagttcatgt acggctccag ggccttcacc aagcaccccg ccgatatccc cgactaccat
3901 aagcagtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
3961 gccgtgaccg tgacccagga cacctccctg gaggacggca ccctgatcta cgaggtgaag
4021 ctccgcggca ccaacttccc tcctgacggc cccgtaatgc agaagaagac tatgggcttg
4081 gaagcggaca ccgagcggtt gtaccccgag gacggcgtgc tgaagggcga cattaagatg
4141 gccctgcgcc tgaagggcgg cggccgctac ctggcacacg tcaggaccac ctacaaggcc
4201 aagaagcccg tgttgatgcc cggcgcctac aacgtcgacc gcaagttgga catcacctcc
4261 cacaacgagg actacaccgt ggtggaacag ttcgaacgct ccgagggccg ccactccacc
4321 ggcgacatgg acgagctgta caagcgaccg gttcacgtgg gaagtggggc tacaaacttc
4381 tcactgctga aacaggccgg ggatgtggag gaaaacccag gaccacacgt gactagtgtg
4441 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
4501 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
4561 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
4621 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
4681 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
4741 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
4801 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
4861 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
4921 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
4981 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
5041 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
5101 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaagaattc
5161 gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
5221 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
5281 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
5341 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
5401 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
5461 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
5521 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
5581 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
5641 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
5701 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
5761 tccccgcatc gataccgtcg acccgggcgg ccgcttcgag cagacatgat aagatacatt
5821 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
5881 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
5941 aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt ttaaagcaag
6001 taaaacctct acaaatgtgg taaaatcgat aaggatcttc ctagagcatg gctacgtaga
6061 taagtagcat ggcgggttaa tcattaacta caaggaaccc ctagtgatgg agttggccac
6121 tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc
6181 gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcatta atgaatcggc
6241 caacgcgcgg ggagaggcgg tttgcgtatt gggcgct
//
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