| 质粒名称: | pDIRECT_26H |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 91150 |
| 存储实验室: | Daniel Voytas |
|
载体骨架基本信息 |
|
| 载体名称: | pTRANS_260d |
| 载体出品公司: | -- |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 植物表达，CRISPR |
| 筛选标记: | Basta |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers |
| 物种来源: | Synthetic |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pDIRECT_26H was a gift from Daniel Voytas (Addgene plasmid # 91150 ; http://n2t.net/addgene:91150 ; RRID:Addgene_91150) |
| 参考文献部分: | A multi-purpose toolkit to enable advanced genome engineering in plants. Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. 10.1105/tpc.16.00922 PubMed 28522548 |
买家导航
pDIRECT_26H载体 pDIRECT26H质粒基本信息
pDIRECT_26H质粒图谱 pDIRECT26H载体图谱和pDIRECT_26H载体序列 pDIRECT26H质粒序列多克隆位点信息
pDIRECT_26H质粒 pDIRECT26H载体简介
pDIRECT_26H质粒序列 pDIRECT26H载体序列
LOCUS pDIRECT_26H 19461 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Direct Cloning, Type: T-DNA, Engineering Reagent:
ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers, Plant
Selection: PvUbi2:bar.
ACCESSION .
VERSION .
KEYWORDS pDIRECT_26H
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 19461)
AUTHORS Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E,
Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
TITLE A multi-purpose toolkit to enable advanced genome engineering in
plants.
JOURNAL Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi:
10.1105/tpc.16.00922.
PUBMED 28522548
REFERENCE 2 (bases 1 to 19461)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..19461
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS complement(291..1085)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
misc_feature 1510..1534
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
polyA_signal 1612..1786
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
CDS complement(1793..2344)
/codon_start=1
/gene="Streptomyces hygroscopicus bar"
/product="phosphinothricin acetyltransferase"
/label=BlpR
/note="confers resistance to bialophos or phosphinothricin"
/translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
WQLDFSLPVPPRPVLPVTEI"
protein_bind 4284..4305
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 4320..4350
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4358..4374
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 4363..4385
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 4382..4398
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 4382..4398
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
CDS 7015..7071
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 7072..11175
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
CDS 11176..11223
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/label=nucleoplasmin NLS
/translation="KRPAATKKAGQAKKKK"
CDS complement(12225..12236)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
protein_bind 13508..13522
/label=Csy4 site
/bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
/note="cleavage site for the Csy4 endoribonuclease
(Haurwitz et al., 2010)"
misc_RNA 13523..13598
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
protein_bind 13604..13618
/label=Csy4 site
/bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
/note="cleavage site for the Csy4 endoribonuclease
(Haurwitz et al., 2010)"
CDS 13938..14243
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
primer_bind 14122..14141
/label=ccdB-fwd
/note="ccdB gene, forward primer"
polyA_signal 14271..14445
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
primer_bind complement(14472..14489)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(14472..14488)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(14481..14503)
/label=M13/pUC Forward
/note="In lacZ gene"
misc_feature 14691..14715
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
CDS 16016..16645
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 StaA
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
CDS 17074..18147
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 RepA
/translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
LVMRYRNLIEGEASAGS"
rep_origin 18213..18407
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
primer_bind complement(18643..18665)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
misc_feature 18751..18891
/label=bom
/note="basis of mobility region from pBR322"
primer_bind 18806..18825
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind complement(18906..18923)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(19077..204)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(19157..19176)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
61 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
121 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
181 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
241 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
301 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
361 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
421 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
481 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
541 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
601 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
661 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
721 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
781 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
841 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
901 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
961 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
1021 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
1081 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
1141 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
1201 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
1261 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
1321 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
1381 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
1441 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
1501 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
1561 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat
1621 tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa
1681 tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac
1741 cctaattccc ttatctggga actactcaca cattattatg gagaaactcg agtcaaatct
1801 cggtgacggg caggaccgga cggggcggta ccggcaggct gaagtccagc tgccagaaac
1861 ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg cagcatgccg cggggggcat
1921 atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg cagcccgatg acagcgacca
1981 cgctcttgaa gccctgtgcc tccagggact tcagcagatg ggtgtagagc gtggagccca
2041 gtcccgtccg ctggtggcgg gggctgacgt acacggtgga ctcggccgtc cagtcgtagg
2101 cgttgcgtgc cttccaggga cccgcgtagg cgatgccggc gacctcgccg tccacctcgg
2161 cgacgagcca gggatagcgc tcccgcagac ggacgaggtc gtccgtccac tcctgcggtt
2221 cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat gtagtggttg acgatggtgc
2281 agaccgccgg catgtccgcc tcggtggcac ggcggatgtc ggccgggcgt cgttctgggc
2341 tcataagctt ctgcaaaaga gaaccagaca acagggtaag tgcctagcag taaacaaaca
2401 gaactcatca caagcaaaca gcaacatcat attcatacca acaggtcatg tgtgttcatc
2461 acatcattag tactaagcat gccatcatcc aagtatatca aagtaagggc aaagagcatg
2521 catgatcatc aggtgcacaa aagaatcatc aaattgtagc agtacatatc ttcatctatc
2581 atgcatatct atccataaca ggacgatgca tgttgaccag gtaaaagcta caggatccta
2641 taggaacagc agcgtatata tcttcaccaa tcttagcatc ttaatcatgt ggcacatgca
2701 gtttcaattt aagcacatga gctagttgat tatgaggtac cagagaatca tcaaactgat
2761 gtagcagcat atatcctcat ctatcatgca atctaatcta atctaactaa acaggaaagg
2821 tgtgctattc agttaaaagc taccgcatca tatacaaacg gcagcataag aaaaagcata
2881 atcatcttaa tcatgcaaca aacgcagatt cataataagc ccaagagcta gcttgtgatg
2941 atcttattct actctgatct acagcaatca gataacgacc taaccttgca catggcaaca
3001 aaacaatcga tcggacgaat cagttgtttg ttcctagcta gcaccatcga accagataat
3061 agatgcacgt acagatcccg aaaacgaacc caaaaacagg gcagacctag ctgaacctag
3121 gcagcgaccc agcagatcgt gagaacgatc tcatctacga acagcctaga agcaacccca
3181 cgattcccgg acaaacgacc taaaatcccc acaaatcaca tgagcatgac aggataaaca
3241 gcggaaccga tcagatctac acgaaaaccc cacctcccag ccacccacga tcaggaaaca
3301 cgcggatcta gcatgatttc gtcaacgcct cagcctagtt cctagccaca gaccaagcag
3361 aaccaccaaa ccacgccgag cgaggagatg gggcaagagg acgggggaga cgatcgccgt
3421 accttgaagc gggggaagga tcgccgaggg tcgcgaggag agcaatttgg atttggaacc
3481 cgggggttgt gcgctccgaa cgatgagacg atgtgagatt gtgggaagag gcgcggaggg
3541 ccctgtattt atgggctgcg acggggggag gagaggtggg gagggttggg gaaggaatcc
3601 cccacccgtg ccgtgacggt tccgggccgt gtgagaggag cccgctcgtc tccgccacgc
3661 aatttccgcg atcggagcgg agctttcgag aggcggctgg atggttggtg gccgttagat
3721 ttgtagacgc cgttaacgcc tcgcctccac cgggaagagt tttgagcagc cgcttatgac
3781 aatggcttaa cgacgttaga cggagcgtta gtggcaggcc atgatagaat agacgtattg
3841 caatgggatt ataattaaat aaataagaat ataataagat atggcaagtc ggcactcatg
3901 acatggtctt cgaaatgata gtgctcactt tcttagccga gaaagttgac gcgactgatt
3961 tagaagttaa gatattattt ctctcttctt ttctttcctc gtcatataag gatgaaataa
4021 actttagaga ttgccggtag tgattttgga tttcggcgat caggcttggt ttgccggttt
4081 cggacggtgt gccttaggcc acccgcagtg tatcttgtaa tgttcaaccg ataagcaagg
4141 gtggggctca agcaagtagt aaacaactat gtcaaatgtc accatggtta tggtcttgtt
4201 tagttggctt cttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
4261 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
4321 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
4381 acaggaaaca gctatgacat gattacgaat tcgagctcgg tacccgggga tctgcaggcg
4441 cgcctgcagt gcagcgtgac ccggtcgtgc ccctctctag agataatgag cattgcatgt
4501 ctaagttata aaaaattacc acatattttt tttgtcacac ttgtttgaag tgcagtttat
4561 ctatctttat acatatattt aaactttact ctacgaataa tataatctat agtactacaa
4621 taatatcagt gttttagaga atcatataaa tgaacagtta gacatggtct aaaggacaat
4681 tgagtatttt gacaacagga ctctacagtt ttatcttttt agtgtgcatg tgttctcctt
4741 tttttttgca aatagcttca cctatataat acttcatcca ttttattagt acatccattt
4801 agggtttagg gttaatggtt tttatagact aattttttta gtacatctat tttattctat
4861 tttagcctct aaattaagaa aactaaaact ctattttagt ttttttattt aataatttag
4921 atataaaata gaataaaata aagtgactaa aaattaaaca aatacccttt aagaaattaa
4981 aaaaactaag gaaacatttt tcttgtttcg agtagataat gccagcctgt taaacgccgt
5041 cgacgagtct aacggacacc aaccagcgaa ccagcagcgt cgcgtcgggc caagcgaagc
5101 agacggcacg gcatctctgt cgctgcctct ggacccctct cgagagttcc gctccaccgt
5161 tggacttgct ccgctgtcgg catccagaaa ttgcgtggcg gagcggcaga cgtgagccgg
5221 cacggcaggc ggcctcctcc tcctctcacg gcaccggcag ctacggggga ttcctttccc
5281 accgctcctt cgctttccct tcctcgcccg ccgtaataaa tagacacccc ctccacaccc
5341 tctttcccca acctcgtgtt gttcggagcg cacacacaca caaccagatc tcccccaaat
5401 ccacccgtcg gcacctccgc ttcaaggtac gccgctcgtc ctcccccccc ccctctctac
5461 cttctctaga tcggcgttcc ggtccatggt tagggcccgg tagttctact tctgttcatg
5521 tttgtgttag atccgtgttt gtgttagatc cgtgctgcta gcgttcgtac acggatgcga
5581 cctgtacgtc agacacgttc tgattgctaa cttgccagtg tttctctttg gggaatcctg
5641 ggatggctct agccgttccg cagacgggat cgatttcatg attttttttg tttcgttgca
5701 tagggtttgg tttgcccttt tcctttattt caatatatgc cgtgcacttg tttgtcgggt
5761 catcttttca tgcttttttt tgtcttggtt gtgatgatgt ggtctggttg ggcggtcgtt
5821 ctagatcgga gtagaattaa ttctgtttca aactacctgg tggatttatt aattttggat
5881 ctgtatgtgt gtgccataca tattcatagt tacgaattga agatgatgga tggaaatatc
5941 gatctaggat aggtatacat gttgatgcgg gttttactga tgcatataca gagatgcttt
6001 ttgttcgctt ggttgtgatg atgtggtgtg gttgggcggt cgttcattcg ttctagatcg
6061 gagtagaata ctgtttcaaa ctacctggtg tatttattaa ttttggaact gtatgtgtgt
6121 gtcatacatc ttcatagtta cgagtttaag atggatggaa atatcgatct aggataggta
6181 tacatgttga tgtgggtttt actgatgcat atacatgatg gcatatgcag catctattca
6241 tatgctctaa ccttgagtac ctatctatta taataaacaa gtatgtttta taattatttt
6301 gatcttgata tacttggatg atggcatatg cagcagctat atgtggattt ttttagccct
6361 gccttcatac gctatttatt tgcttggtac tgtttctttt gtcgatgctc accctgttgt
6421 ttggtgttac ttcctgcagg aagcatggac cactacctcg acatcaggct caggccagac
6481 ccagagttcc caccagccca gctcatgtcc gtcctcttcg gcaagctcca ccaggccctc
6541 gtggcccagg gcggcgacag gatcggcgtg tccttcccag acctcgacga gtccaggtcc
6601 aggctcggcg agaggctccg catccacgcc tccgccgacg acctcagggc cctcctcgcc
6661 aggccgtggc tggagggcct cagggaccac ctccagttcg gcgagccagc cgtggtgcca
6721 cacccaaccc catacaggca agtgtccagg gtgcaagcca agtccaaccc agagaggctc
6781 aggaggaggc tcatgaggag gcacgacctc tccgaggaag aggccaggaa gcgcatccca
6841 gacaccgtgg ccagggccct cgacctccca ttcgtgaccc tcaggtccca gtccaccggc
6901 cagcacttcc gcctcttcat caggcacggc ccactccagg tgaccgccga ggagggcggc
6961 tttacctgct acggcctctc caagggcggc ttcgtgccgt ggttcggctc cggcgccacc
7021 aacttctccc tcctcaagca agccggcgac gtggaggaga acccaggccc aatggacaag
7081 aagtactcga tcggcctcga catcgggacg aactcagttg gctgggccgt gatcaccgac
7141 gagtacaagg tgccctctaa gaagttcaag gtcctgggga acaccgaccg ccattccatc
7201 aagaagaacc tcatcggcgc tctcctgttc gacagcgggg agaccgctga ggctacgagg
7261 ctcaagagaa ccgctaggcg ccggtacacg agaaggaaga acaggatctg ctacctccaa
7321 gagattttct ccaacgagat ggccaaggtt gacgattcat tcttccaccg cctggaggag
7381 tctttcctcg tggaggagga taagaagcac gagcggcatc ccatcttcgg caacatcgtg
7441 gacgaggttg cctaccacga gaagtaccct acgatctacc atctgcggaa gaagctcgtg
7501 gactccaccg ataaggcgga cctcagactg atctacctcg ctctggccca catgatcaag
7561 ttccgcggcc atttcctgat cgagggggat ctcaacccag acaacagcga tgttgacaag
7621 ctgttcatcc aactcgtgca gacctacaac caactcttcg aggagaaccc gatcaacgcc
7681 tctggcgtgg acgcgaaggc tatcctgtcc gcgaggctct cgaagtccag gaggctggag
7741 aacctgatcg ctcagctccc aggcgagaag aagaacggcc tgttcgggaa cctcatcgct
7801 ctcagcctgg ggctcacccc gaacttcaag tcgaacttcg atctcgctga ggacgccaag
7861 ctgcaactct ccaaggacac ctacgacgat gacctcgata acctcctggc ccagatcggc
7921 gatcaatacg cggacctgtt cctcgctgcc aagaacctgt cggacgccat cctcctgtca
7981 gatatcctcc gcgtgaacac cgagatcacg aaggctccac tctctgcctc catgatcaag
8041 cgctacgacg agcaccatca ggatctgacc ctcctgaagg cgctggtccg ccaacagctc
8101 ccggagaagt acaaggagat tttcttcgat cagtcgaaga acggctacgc tgggtacatc
8161 gacggcgggg cctcacaaga ggagttctac aagttcatca agccaatcct ggagaagatg
8221 gacggcacgg aggagctcct ggtgaagctc aacagggagg acctcctgcg gaagcagaga
8281 accttcgata acggcagcat cccccaccaa atccatctcg gggagctgca cgccatcctg
8341 agaaggcaag aggacttcta ccctttcctc aaggataacc gggagaagat cgagaagatc
8401 ctgaccttca gaatcccata ctacgtcggc cctctcgcgc gggggaactc aagattcgct
8461 tggatgaccc gcaagtctga ggagaccatc acgccgtgga acttcgagga ggtggtggac
8521 aagggcgcta gcgctcagtc gttcatcgag aggatgacca acttcgacaa gaacctgccc
8581 aacgagaagg tgctccctaa gcactcgctc ctgtacgagt acttcaccgt ctacaacgag
8641 ctcacgaagg tgaagtacgt caccgagggc atgcgcaagc cagcgttcct gtccggggag
8701 cagaagaagg ctatcgtgga cctcctgttc aagaccaacc ggaaggtcac ggttaagcaa
8761 ctcaaggagg actacttcaa gaagatcgag tgcttcgatt cggtcgagat cagcggcgtt
8821 gaggaccgct tcaacgccag cctcgggacc taccacgatc tcctgaagat catcaaggat
8881 aaggacttcc tggacaacga ggagaacgag gatatcctgg aggacatcgt gctgaccctc
8941 acgctgttcg aggacaggga gatgatcgag gagcgcctga agacgtacgc ccatctcttc
9001 gatgacaagg tcatgaagca actcaagcgc cggagataca ccggctgggg gaggctgtcc
9061 cgcaagctca tcaacggcat ccgggacaag cagtccggga agaccatcct cgacttcctc
9121 aagagcgatg gcttcgccaa caggaacttc atgcaactga tccacgatga cagcctcacc
9181 ttcaaggagg atatccaaaa ggctcaagtg agcggccagg gggactcgct gcacgagcat
9241 atcgcgaacc tcgctggctc ccccgcgatc aagaagggca tcctccagac cgtgaaggtt
9301 gtggacgagc tcgtgaaggt catgggccgg cacaagcctg agaacatcgt catcgagatg
9361 gccagagaga accaaaccac gcagaagggg caaaagaact ctagggagcg catgaagcgc
9421 atcgaggagg gcatcaagga gctggggtcc caaatcctca aggagcaccc agtggagaac
9481 acccaactgc agaacgagaa gctctacctg tactacctcc agaacggcag ggatatgtac
9541 gtggaccaag agctggatat caaccgcctc agcgattacg acgtcgatca tatcgttccc
9601 cagtctttcc tgaaggatga ctccatcgac aacaaggtcc tcaccaggtc ggacaagaac
9661 cgcggcaagt cagataacgt tccatctgag gaggtcgtta agaagatgaa gaactactgg
9721 aggcagctcc tgaacgccaa gctgatcacg caaaggaagt tcgacaacct caccaaggct
9781 gagagaggcg ggctctcaga gctggacaag gccggcttca tcaagcggca gctggtcgag
9841 accagacaaa tcacgaagca cgttgcgcaa atcctcgact ctcggatgaa cacgaagtac
9901 gatgagaacg acaagctgat cagggaggtt aaggtgatca ccctgaagtc taagctcgtc
9961 tccgacttca ggaaggattt ccagttctac aaggttcgcg agatcaacaa ctaccaccat
10021 gcccatgacg cttacctcaa cgctgtggtc ggcaccgctc tgatcaagaa gtacccaaag
10081 ctggagtccg agttcgtgta cggggactac aaggtttacg atgtgcgcaa gatgatcgcc
10141 aagtcggagc aagagatcgg caaggctacc gccaagtact tcttctactc aaacatcatg
10201 aacttcttca agaccgagat cacgctggcc aacggcgaga tccggaagag accgctcatc
10261 gagaccaacg gcgagacggg ggagatcgtg tgggacaagg gcagggattt cgcgaccgtc
10321 cgcaaggttc tctccatgcc ccaggtgaac atcgtcaaga agaccgaggt ccaaacgggc
10381 gggttctcaa aggagtctat cctgcctaag cggaacagcg acaagctcat cgccagaaag
10441 aaggactggg acccaaagaa gtacggcggg ttcgacagcc ctaccgtggc ctactcggtc
10501 ctggttgtgg cgaaggttga gaagggcaag tccaagaagc tcaagagcgt gaaggagctc
10561 ctggggatca ccatcatgga gaggtccagc ttcgagaaga acccaatcga cttcctggag
10621 gccaagggct acaaggaggt gaagaaggac ctgatcatca agctcccgaa gtactctctc
10681 ttcgagctgg agaacggcag gaagagaatg ctggcttccg ctggcgagct ccagaagggg
10741 aacgagctcg cgctgccaag caagtacgtg aacttcctct acctggcttc ccactacgag
10801 aagctcaagg gcagcccgga ggacaacgag caaaagcagc tgttcgtcga gcagcacaag
10861 cattacctcg acgagatcat cgagcaaatc tccgagttca gcaagcgcgt gatcctcgcc
10921 gacgcgaacc tggataaggt cctctccgcc tacaacaagc accgggacaa gcccatcaga
10981 gagcaagcgg agaacatcat ccatctcttc accctgacga acctcggcgc tcctgctgct
11041 ttcaagtact tcgacaccac gatcgatcgg aagagataca cctccacgaa ggaggtcctg
11101 gacgcgaccc tcatccacca gtcgatcacc ggcctgtacg agacgaggat cgacctctca
11161 caactcggcg gggataagag acccgcagca accaagaagg cagggcaagc aaagaagaag
11221 aagtgactcg agtatatgaa gatgaagatg aaatatttgg tgtgtcaaat aaaaagcttg
11281 tgtgcttaag tttgtgtttt tttcttggct tgttgtgtta tgaatttgtg gctttttcta
11341 atattaaatg aatgtaagat cacattataa tgaataaaca aatgtttcta taatccattg
11401 tgaatgtttt gttggatctc ttctgcagca tataactact gtatgtgcta tggtatggac
11461 tatggaatat gattaaagat aaggagctcg ttaaccggtg acggacggcg cgccgaagag
11521 cacgtcagtg tttggtttcc actagcacga gtagcgcaat cagaaaattt tcaatgcatg
11581 aagtactaaa cgaagtttat ttagaaattt ttttaagaaa tgagtgtaat tttttgcgac
11641 gaatttaatg acaataatta atcgatgatt gcctacagta atgctacagt aaccaacctc
11701 taatcatgcg tcgaatgcgt cattagattc gtctcgcaaa atagcacaag aattatgaaa
11761 ttaattttac aaactatttt tatttaatac taataattaa ctgtcaaagt ttgtgctact
11821 cgcaagagta gcgcgaacca aacacggcct ggaggagcac ggtaacggcg tcgacaaact
11881 aacggccacc acccgccaac gcaaaggaga cggatgagag ttgacttctt gacggttctc
11941 cacccctctg tctctctgtc actgggccct gggtccccct ctcgaaagtt cctctggccg
12001 aaattgcgcg gcggagacga ggcgggcgga accgtcacgg cagaggattc cttccccacc
12061 ctgcctggcc cggccatata taaacagcca ccgcccctcc ccgttcccca tcgcgtctcg
12121 tctcgtgttg ttcccagaac acaaccaaaa tccaaatcct cctcctcctc ccgagcctcg
12181 tcgatccctc acccgcttca aggtacggcg atcctcctct cccttctccc ctcgatcgat
12241 tatgcgtgtt ccgtttccgt ttccgatcga gcgaatcgat ggttaggacc catgggggac
12301 ccatggggtg tcgtgtggtg gtctggtttg atccgcgata tttctccgtt cgtagtgtag
12361 atctgatcga atccctggtg aaatcgttga tcgtgctatt cgtgtgaggg ttcttaggtt
12421 tggagttgtg gaggtagttc tgatcggttt gtaggtgaga ttttccccat gattttgctt
12481 ggctcgtttg tcttggttag attagatctg cccgcatttt gttcgatatt tctgatgcag
12541 atatgatgaa taatttcgtc cttgtatccc gcgtccgtat gtgtattaag tttgcaggtc
12601 ctagttaggt ttttcctact gatttgtctt atccattctg tttagcttgc aaggtttggt
12661 aatggtccgg catgtttgtc tctatagatt agagtagaat aagattatct caacaagctg
12721 ttggcttatc aattttggat ctgcatgtgt ttcgcatcta tatctttgca attaagatgg
12781 tagatggaca tatgctcctg ttgagttgat gttgtacctt ttacctgagg tctgaggaac
12841 atgcatcctc ctgctacttt gtgcttatac agatcatcaa gattatgtag ctaatattcg
12901 atcagtttct agtatctaca tggtaaactt gcatgcactt gctacttatt tttgatatac
12961 ttggatgata acatatgctg ctggttgatt cctacctaca tgatgaacat tttacaggcc
13021 attagtgtct gtctgtatgt gttgttcctg tttgcttcag tctatttctg tttcattcct
13081 agtttattgg ttctctgcta gatacttacc ctgctgggct tagttatcat cttatctcga
13141 atgcattttc atgtttatag atgaatatac actcagatag gtgtagatgt atgctactgt
13201 ttctctacgt tgctgtaggt tttacctgtg gcaactgcat actcctgttg cttcgctaga
13261 tatgtatgtg cttatataga ttaagatatg tgtgatggtt ctttagtata tctgatgatc
13321 atgtatgctc ttttaacttc ttgctacact tggtaacatg ctgtgatgct gtttgttgat
13381 tctgtagcac taccaatgat gaccttatct ctctttgtat atgatgtttc tgtttgtttg
13441 aggcttgtgt tactgctagt tacttaccct gttgcctggc taatcttctg cagatcctgc
13501 aggttcactg ccgtataggc aggttttaga gctagaaata gcaagttaaa ataaggctag
13561 tccgttatca acttgaaaaa gtggcaccga gtcggtgcgt tcactgccgt ataggcagaa
13621 gccagataac agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat
13681 atgtataccc gaagtatgtc aaaaggaggt atgctatgaa gcagcgtatt acagtgacag
13741 ttgacagcga cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt
13801 aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa
13861 tcaggaaggg atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga
13921 gaacaggggc tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc
13981 gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc
14041 ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc
14101 atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtttccg
14161 ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta
14221 acctgatgtt ctggggaata taagctcttc agtcgatcga caagctcgag tttctccata
14281 ataatgtgtg agtagttccc agataaggga attagggttc ctatagggtt tcgctcatgt
14341 gttgagcata taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa
14401 aatttctaat tcctaaaacc aaaatccagt actaaaatcc agatcccccg aattagagct
14461 ctaccggtgg cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
14521 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
14581 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatgcta gagcagcttg
14641 agcttggatc agattgtcgt ttcccgcctt cagtttaaac tatcagtgtt tgacaggata
14701 tattggcggg taaacctaag agaaaagagc gtttattaga ataatcggat atttaaaagg
14761 gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat gccaaccaca gggttcccct
14821 cgggatcaaa gtactttgat ccaacccctc cgctgctata gtgcagtcgg cttctgacgt
14881 tcagtgcagc cgtcttctga aaacgacatg tcgcacaagt cctaagttac gcgacaggct
14941 gccgccctgc ccttttcctg gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat
15001 acttgcgact agaaccggag acattacgcc atgaacaaga gcgccgccgc tggcctgctg
15061 ggctatgccc gcgtcagcac cgacgaccag gacttgacca accaacgggc cgaactgcac
15121 gcggccggct gcaccaagct gttttccgag aagatcaccg gcaccaggcg cgaccgcccg
15181 gagctggcca ggatgcttga ccacctacgc cctggcgacg ttgtgacagt gaccaggcta
15241 gaccgcctgg cccgcagcac ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc
15301 ggcgcgggcc tgcgtagcct ggcagagccg tgggccgaca ccaccacgcc ggccggccgc
15361 atggtgttga ccgtgttcgc cggcattgcc gagttcgagc gttccctaat catcgaccgc
15421 acccggagcg ggcgcgaggc cgccaaggcc cgaggcgtga agtttggccc ccgccctacc
15481 ctcaccccgg cacagatcgc gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg
15541 aaagaggcgg ctgcactgct tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc
15601 agcgaggaag tgacgcccac cgaggccagg cggcgcggtg ccttccgtga ggacgcattg
15661 accgaggccg acgccctggc ggccgccgag aatgaacgcc aagaggaaca agcatgaaac
15721 cgcaccagga cggccaggac gaaccgtttt tcattaccga agagatcgag gcggagatga
15781 tcgcggccgg gtacgtgttc gagccgcccg cgcacggctc aaccgtgcgg ctgcatgaaa
15841 tcctggccgg tttgtctgat gccaagctgg cggcctggcc ggccagcttg gccgctgaag
15901 aaaccgagcg ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg
15961 cggtcgctgc gtatatgatg cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa
16021 ggttatcgct gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg caacccatct
16081 agcccgcgcc ctgcaactcg ccggggccga tgttctgtta gtcgattccg atccccaggg
16141 cagtgcccgc gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga
16201 ccgcccgacg attgaccgcg acgtgaaggc catcggccgg cgcgacttcg tagtgatcga
16261 cggagcgccc caggcggcgg acttggctgt gtccgcgatc aaggcagccg acttcgtgct
16321 gattccggtg cagccaagcc cttacgacat atgggccacc gccgacctgg tggagctggt
16381 taagcagcgc attgaggtca cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc
16441 gatcaaaggc acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc
16501 cattcttgag tcccgtatca cgcagcgcgt gagctaccca ggcactgccg ccgccggcac
16561 aaccgttctt gaatcagaac ccgagggcga cgctgcccgc gaggtccagg cgctggccgc
16621 tgaaattaaa tcaaaactca tttgagttaa tgaggtaaag agaaaatgag caaaagcaca
16681 aacacgctaa gtgccggccg tccgagcgca cgcagcagca aggctgcaac gttggccagc
16741 ctggcagaca cgccagccat gaagcgggtc aactttcagt tgccggcgga ggatcacacc
16801 aagctgaaga tgtacgcggt acgccaaggc aagaccatta ccgagctgct atctgaatac
16861 atcgcgcagc taccagagta aatgagcaaa tgaataaatg agtagatgaa ttttagcggc
16921 taaaggaggc ggcatggaaa atcaagaaca accaggcacc gacgccgtgg aatgccccat
16981 gtgtggagga acgggcggtt ggccaggcgt aagcggctgg gttgcctgcc ggccctgcaa
17041 tggcactgga acccccaagc ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg
17101 gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc
17161 gcccagcggc aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct
17221 gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag
17281 ccgcccaagg gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc
17341 cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga
17401 gctggcgagg tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg
17461 gccggcatgg ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc
17521 gaatccatga accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca
17581 cacgttgcgg acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac
17641 ctggtagaaa cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag
17701 gccaagaacg gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag
17761 atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg
17821 taccgcgaga tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt
17881 ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag
17941 gcagaagcca gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc
18001 aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat
18061 ttgaaggagg aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc
18121 gagggcgaag catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta
18181 gcaggggaaa aaggtcgaaa aggcctcttt cctgtggata gcacgtacat tgggaaccca
18241 aagccgtaca ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac
18301 cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac
18361 tctttaaaac ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag
18421 cgcacagccc aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc
18481 gccgcttcgc gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca
18541 ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat
18601 caaggcaccc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
18661 cccggaaacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
18721 cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag
18781 cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
18841 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gccctcttcc
18901 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
18961 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
19021 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
19081 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
19141 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
19201 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
19261 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
19321 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
19381 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
19441 aggattagca gagcgaggta t
//
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pDIRECT_26H质粒 pDIRECT26H载体应用举例
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