pAXY0001

LOCUS       pAXY0001               14360 bp ds-DNA     circular SYN 12-APR-2022
DEFINITION  binary plant vector expressing eYGFPuv and GUS, both driven by a 35S
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pAXY0001
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14360)
  AUTHORS   Yuan G, Lu H, Tang D, Hassan MM, Li Y, Chen JG, Tuskan GA, Yang X
  TITLE     Expanding the application of a UV-visible reporter for transient 
            gene expression and stable transformation in plants.
  JOURNAL   Hortic Res. 2021 Nov 1;8(1):234. doi: 10.1038/s41438-021-00663-3.
  PUBMED    34719678
REFERENCE   2  (bases 1 to 14360)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 12, 2022 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..14360
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             289..1314
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    1355..1529
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    1607..1631
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             2056..2850
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      2937..3525
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3426..3445
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3679..3696
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3711..3851
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3777..3796)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3937..3959
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4195..4389
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(4455..5528)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(5957..6586)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    7887..7911
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      7933..8185
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(8217..8234)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(join(8241..10058,10249..10263))
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUSPlus(TM)
                     /note="codon-optimized Staphylococcus gusA gene with a 
                     catalase intron to ensure expression in plants but not 
                     bacteria"
                     /translation="MVDLRNRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYES
                     KLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAI
                     VYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGL
                     GKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAE
                     TVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEP
                     FGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFR
                     TAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHH
                     QDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLF
                     VMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITE
                     YGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRV
                     QGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
     intron          10059..10248
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     promoter        complement(10280..10625)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     10987..11009
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11001..11018
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11002..11018
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        11533..11878
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(13601..13617)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13601..13617)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13614..13636)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13625..13641
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(13649..13679)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13694..13715
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        13906..222
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
ORIGIN
        1 aaggctatcg ttcaagatgc ctctgccgac agtggtccca aagatggacc cccacccacg
       61 aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt
      121 gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc
      181 tctatataag gaagttcatt tcatttggag aggacacgct gaaatcacca gtctctctct
      241 acaaatctat ctctctcgag ctttcgcaga tccggggggc aatgagatat gaaaaagcct
      301 gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac
      361 ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt
      421 ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat
      481 cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggagtttagc
      541 gagagcctga cctattgcat ctcccgccgt tcacagggtg tcacgttgca agacctgcct
      601 gaaaccgaac tgcccgctgt tctacaaccg gtcgcggagg ctatggatgc gatcgctgcg
      661 gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac
      721 actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact
      781 gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg
      841 gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc
      901 ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat
      961 tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag
     1021 cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccacg actccgggcg
     1081 tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat
     1141 gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc
     1201 gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta
     1261 ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagaa atagagtaga
     1321 tgccgaccgg gatctgtcga tcgacaagct cgagtttctc cataataatg tgtgagtagt
     1381 tcccagataa gggaattagg gttcctatag ggtttcgctc atgtgttgag catataagaa
     1441 acccttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc taattcctaa
     1501 aaccaaaatc cagtactaaa atccagatcc cccgaattaa ttcggcgtta attcagtaca
     1561 ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt acaccacaat
     1621 atatcctgcc accagccagc caacagctcc ccgaccggca gctcggcaca aaatcaccac
     1681 tcgatacagg cagcccatca gtccgggacg gcgtcagcgg gagagccgtt gtaaggcggc
     1741 agactttgct catgttaccg atgctattcg gaagaacggc aactaagctg ccgggtttga
     1801 aacacggatg atctcgcgga gggtagcatg ttgattgtaa cgatgacaga gcgttgctgc
     1861 ctgtgatcac cgcggtttca aaatcggctc cgtcgatact atgttatacg ccaactttga
     1921 aaacaacttt gaaaaagctg ttttctggta tttaaggttt tagaatgcaa ggaacagtga
     1981 attggagttc gtcttgttat aattagcttc ttggggtatc tttaaatact gtagaaaaga
     2041 ggaaggaaat aataaatggc taaaatgaga atatcaccgg aattgaaaaa actgatcgaa
     2101 aaataccgct gcgtaaaaga tacggaagga atgtctcctg ctaaggtata taagctggtg
     2161 ggagaaaatg aaaacctata tttaaaaatg acggacagcc ggtataaagg gaccacctat
     2221 gatgtggaac gggaaaagga catgatgcta tggctggaag gaaagctgcc tgttccaaag
     2281 gtcctgcact ttgaacggca tgatggctgg agcaatctgc tcatgagtga ggccgatggc
     2341 gtcctttgct cggaagagta tgaagatgaa caaagccctg aaaagattat cgagctgtat
     2401 gcggagtgca tcaggctctt tcactccatc gacatatcgg attgtcccta tacgaatagc
     2461 ttagacagcc gcttagccga attggattac ttactgaata acgatctggc cgatgtggat
     2521 tgcgaaaact gggaagaaga cactccattt aaagatccgc gcgagctgta tgatttttta
     2581 aagacggaaa agcccgaaga ggaacttgtc ttttcccacg gcgacctggg agacagcaac
     2641 atctttgtga aagatggcaa agtaagtggc tttattgatc ttgggagaag cggcagggcg
     2701 gacaagtggt atgacattgc cttctgcgtc cggtcgatca gggaggatat cggggaagaa
     2761 cagtatgtcg agctattttt tgacttactg gggatcaagc ctgattggga gaaaataaaa
     2821 tattatattt tactggatga attgttttag tacctagaat gcatgaccaa aatcccttaa
     2881 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     2941 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     3001 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     3061 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     3121 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     3181 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     3241 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     3301 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     3361 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     3421 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     3481 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     3541 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     3601 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     3661 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     3721 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
     3781 atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg
     3841 tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     3901 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     3961 tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt gatgtgggcg ccggcggtcg
     4021 agtggcgacg gcgcggcttg tccgcgccct ggtagattgc ctggccgtag gccagccatt
     4081 tttgagcggc cagcggccgc gataggccga cgcgaagcgg cggggcgtag ggagcgcagc
     4141 gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc gctggccaga cagttatgca
     4201 caggccaggc gggttttaag agttttaata agttttaaag agttttaggc ggaaaaatcg
     4261 ccttttttct cttttatatc agtcacttac atgtgtgacc ggttcccaat gtacggcttt
     4321 gggttcccaa tgtacgggtt ccggttccca atgtacggct ttgggttccc aatgtacgtg
     4381 ctatccacag gaaagagacc ttttcgacct ttttcccctg ctagggcaat ttgccctagc
     4441 atctgctccg tacattagga accggcggat gcttcgccct cgatcaggtt gcggtagcgc
     4501 atgactagga tcgggccagc ctgccccgcc tcctccttca aatcgtactc cggcaggtca
     4561 tttgacccga tcagcttgcg cacggtgaaa cagaacttct tgaactctcc ggcgctgcca
     4621 ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt
     4681 gccaggcggt agagaaaacg gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc
     4741 gtcagcacgt ccgggttctt gccttctgtg atctcgcggt acatccaatc agctagctcg
     4801 atctcgatgt actccggccg cccggtttcg ctctttacga tcttgtagcg gctaatcaag
     4861 gcttcaccct cggataccgt caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg
     4921 gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg
     4981 ccatcggctc gccggcagaa cttgagtacg tccgcaacgt gtggacggaa cacgcggccg
     5041 ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc
     5101 atcagtacca ggtcgtaatc ccacacactg gccatgccgg ccggccctgc ggaaacctct
     5161 acgtgcccgt ctggaagctc gtagcggatc acctcgccag ctcgtcggtc acgcttcgac
     5221 agacggaaaa cggccacgtc catgatgctg cgactatcgc gggtgcccac gtcatagagc
     5281 atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg gcttcctaat cgacggcgca
     5341 ccggctgccg gcggttgccg ggattctttg cggattcgat cagcggccgc ttgccacgat
     5401 tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac
     5461 ttctccacca ggtcatcacc cagcgccgcg ccgatttgta ccgggccgga tggtttgcga
     5521 ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc attgcagggc cggcaggcaa
     5581 cccagccgct tacgcctggc caaccgcccg ttcctccaca catggggcat tccacggcgt
     5641 cggtgcctgg ttgttcttga ttttccatgc cgcctccttt agccgctaaa attcatctac
     5701 tcatttattc atttgctcat ttactctggt agctgcgcga tgtattcaga tagcagctcg
     5761 gtaatggtct tgccttggcg taccgcgtac atcttcagct tggtgtgatc ctccgccggc
     5821 aactgaaagt tgacccgctt catggctggc gtgtctgcca ggctggccaa cgttgcagcc
     5881 ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt ttgtgctttt gctcattttc
     5941 tctttacctc attaactcaa atgagttttg atttaatttc agcggccagc gcctggacct
     6001 cgcgggcagc gtcgccctcg ggttctgatt caagaacggt tgtgccggcg gcggcagtgc
     6061 ctgggtagct cacgcgctgc gtgatacggg actcaagaat gggcagctcg tacccggcca
     6121 gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg
     6181 ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg
     6241 cggtggccca tatgtcgtaa gggcttggct gcaccggaat cagcacgaag tcggctgcct
     6301 tgatcgcgga cacagccaag tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc
     6361 gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta
     6421 gcggttgatc ttcccgcacg gccgcccaat cgcgggcact gccctgggga tcggaatcga
     6481 ctaacagaac atcggccccg gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg
     6541 tcttgcctga cccgcctttc tggttaagta cagcgataac cttcatgcgt tccccttgcg
     6601 tatttgttta tttactcatc gcatcatata cgcagcgacc gcatgacgca agctgtttta
     6661 ctcaaataca catcaccttt ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc
     6721 cggccaggcc gccagcttgg catcagacaa accggccagg atttcatgca gccgcacggt
     6781 tgagacgtgc gcgggcggct cgaacacgta cccggccgcg atcatctccg cctcgatctc
     6841 ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct
     6901 tggcgttcat tctcggcggc cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag
     6961 gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac
     7021 agggtcgagc gatgcacgcc aagcagtgca gccgcctctt tcacggtgcg gccttcctgg
     7081 tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga gggtagggcg ggggccaaac
     7141 ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa
     7201 cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg
     7261 gtgtcggccc acggctctgc caggctacgc aggcccgcgc cggcctcctg gatgcgctcg
     7321 gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca
     7381 acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg
     7441 ccggtgatct tctcggaaaa cagcttggtg cagccggccg cgtgcagttc ggcccgttgg
     7501 ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg
     7561 ctcttgttca tggcgtaatg tctccggttc tagtcgcaag tattctactt tatgcgacta
     7621 aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag
     7681 gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg aacgtcagaa gccgactgca
     7741 ctatagcagc ggaggggttg gatcaaagta ctttgatccc gaggggaacc ctgtggttgg
     7801 catgcacata caaatggacg aacggataaa ccttttcacg cccttttaaa tatccgatta
     7861 ttctaataaa cgctcttttc tcttaggttt acccgccaat atatcctgtc aaacactgat
     7921 agtttaattc ccgatctagt aacatagatg acaccgcgcg cgataattta tcctagtttg
     7981 cgcgctatat tttgttttct atcgcgtatt aaatgtataa ttgcgggact ctaatcataa
     8041 aaacccatct cataaataac gtcatgcatt acatgttaat tattacatgc ttaacgtaat
     8101 tcaacagaaa ttatatgata atcatcgcaa gaccggcaac aggattcaat cttaagaaac
     8161 tttattgcca aatgtttgaa cgatcgggga aattcgagct ggtcaccaat tcacacgtga
     8221 tggtgatggt gatggctagc gttcttgtag ccgaaatctg gaatgttggt ccagcgctcg
     8281 cgaaagacgt gcgcggcgag cttcggcttg cggtcacgag tgaacacgcc cttcttgttt
     8341 ccttggacgc gcatcacgcc ctgagaggtc gcgaagtccg cgaagttcca cgcttgctca
     8401 cccacgaagt tctcaaactc atcgaacacg acgtggttcg cctggtagta ctcgacttga
     8461 tattcctcgg tgaacatcac tggatcaatg tcgtgaaagc ccgcaacggt gtctgcgccg
     8521 tactcagtga tcatgatcgg ctttcctggg caacgcttgt tccacgcgtg aaattcctgg
     8581 cggagatgga ctttggccgc ttcgagatca ccgccatcga agtaccatcc gttatagcga
     8641 ttgagcgcga tgacgtcaat cagttcggcg actttgtccg tctccggggt agccatcaca
     8701 aacagcacga tcgtgaccgg acgcttctgt gggtcgagtt ccttggtcag ctccaccaac
     8761 ggcttgaagt actcgtacgc gccctcttcc tcagtcgccg cctcgttggc gatgctccac
     8821 atcacgacgc ttggatggtt cttgtcacga gacaccagtt cacggagaac gtcttgatgg
     8881 tgctcaaacg tccgaatctt ctcccaggta ctgacgcgct cgctgccttc gccgagtccc
     8941 gtggtggcca tgaagttgag gtgcacgcca actgccggag tctcgtcgat cacgaccaga
     9001 ccctcgcgat ccgcaagacg catcaactct tcagagtacg gatagtgtgc ggtccggaag
     9061 ctgttggcgc cgatccattt gaggatattg aaatccatca cattgctcgc ttcgttaaag
     9121 ccacggccgt tgataggagt gtcctcatgt ttgccaaagc ccttgaagta gaacggtttg
     9181 ttgttgatga ggaacttgcc gtcgttgact tccacggtcc gcacgccgaa cggctcttca
     9241 tagacatcga tggtcagtcc gtcgttcacc agttccactt tgatctggta gagatacgtg
     9301 ttcagtggtt cccagaggat gacattcgga atctccacgt taccgctcag gccctcggtg
     9361 cttgcgacca ctttgccttc ctcatccacg accgacactt tcacggtctc ggctttgcct
     9421 tgaaagtcca ccgtataggt cacagtcccg gttgggccat tgaagtcggt cacaaccgag
     9481 atgtcctcga cgtacgtaaa cggggtcgtg tagattttca ccggacggtg caggcctgca
     9541 tagttgaaga agtcgaagtt cggcttgtta cgaatgactt ttccgaggcc ctcttcgtgg
     9601 cgctcgctgt acagccccac cgggagggtg ctatcgtcga ggatgttgtc cacggcgacg
     9661 gtgacgcgat tcatgccatc acgcagcgag ttgttgattt ccgcttcgaa tggcaggaat
     9721 ccgcccttgt gctccacgac cagctcacca ttgacataga caattgcttt gtgagttgca
     9781 gagccgaagc ggagcacgat acgctgatcc ttcagatagg ccggcaccgt gaactcacgt
     9841 tcgtaccaga catatccgat atggttgcgg atttccttgg tcacgccaat gtcattgtaa
     9901 ctgcttggga cggccatact aatagtgtcg gtcagcttgc tttcgtacca cttctcttcc
     9961 agtcctttcc cgtagtccag cttgaagttc cagacgccat tgaggtcgaa gacgccacgg
    10021 gtctcggtgt tgatcgggta cagactagtt cgtcggttct gtaactatca tcatcatcat
    10081 agacacacga aataaagtaa tcagattatc agttaaagct atgtaatatt tacaccataa
    10141 ccaatcaatt aaaaaataga tcagtttaaa gaaagatcaa agctcaaaaa aataaaaaga
    10201 gaaaagggtc ctaaccaaga aaatgaagga gaaaaactag aaatttaccc tcagatctac
    10261 catggtcaag agtcccccgt gttctctcca aatgaaatga acttccttat atagaggaag
    10321 ggtcttgcga aggatagtgg gattgtgcgt catcccttac gtcagtggag atatcacatc
    10381 aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg
    10441 gtgggggtcc atctttggga ccactgtcgg cagaggcatc ttcaacgatg gcctttcctt
    10501 tatcgcaatg atggcatttg taggagccac cttccttttc cactatcttc acaataaagt
    10561 gacagatagc tgggcaatgg aatccgagga ggtttccgga tattaccctt tgttgaaaag
    10621 tctcaattgc cctttggtct tctgagactg tatctttgat atttttggag tagacaagtg
    10681 tgtcgtgctc caccatgttg acgaagattt tcttcttgtc attgagtcgt aagagactct
    10741 gtatgaactg ttcgccagtc tttacggcga gttctgttag gtcctctatt tgaatctttg
    10801 actccatgaa gctaaactga aggcgggaaa cgacaatctg atccaagctc aagctgctct
    10861 agcattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
    10921 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
    10981 ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagcttgca tgcctgcagg
    11041 tccccagatt agccttttca atttcagaaa gaatgctaac ccacagatgg ttagagaggc
    11101 ttacgcagca ggtctcatca agacgatcta cccgagcaat aatctccagg aaatcaaata
    11161 ccttcccaag aaggttaaag atgcagtcaa aagattcagg actaactgca tcaagaacac
    11221 agagaaagat atatttctca agatcagaag tactattcca gtatggacga ttcaaggctt
    11281 gcttcacaaa ccaaggcaag taatagagat tggagtctct aaaaaggtag ttcccactga
    11341 atcaaaggcc atggagtcaa agattcaaat agaggaccta acagaactcg ccgtaaagac
    11401 tggcgaacag ttcatacaga gtctcttacg actcaatgac aagaagaaaa tcttcgtcaa
    11461 catggtggag cacgacacac ttgtctactc caaaaatatc aaagatacag tctcagaaga
    11521 ccaaagggca attgagactt ttcaacaaag ggtaatatcc ggaaacctcc tcggattcca
    11581 ttgcccagct atctgtcact ttattgtgaa gatagtggaa aaggaaggtg gctcctacaa
    11641 atgccatcat tgcgataaag gaaaggccat cgttgaagat gcctctgccg acagtggtcc
    11701 caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc
    11761 ttcaaagcaa gtggattgat gtgatatctc cactgacgta agggatgacg cacaatccca
    11821 ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg agagaacacg
    11881 ggggactcaa acattactca ttcacaaaac catcttaaag caactacaca agtcttgaaa
    11941 ttttctcata ttttctattt actatataaa cttttaatca aatcaagatt aaagttaatt
    12001 aaatgacaac cttcaaaatc gagtcccgga tccacggcaa cctcaacggg gagaagttcg
    12061 agttggttgg aggtggagta ggtgaggagg gtcgcctcga gattgagatg aagactaaag
    12121 ataaaccact ggcattctct cccttcctgc tgaccacttg catgggttac gggttctacc
    12181 acttcgccag cttcccaaag gggattaaga acatctatct tcatgctgca acgaacggag
    12241 gttacaccaa caccaggaag gagatctatg aagacggcgg catcttggag gtcaacttcc
    12301 gttacactta cgagttcaac aagatcatcg gtgacgtcga gtgcattgga catggattcc
    12361 caagtcagag tccgatcttc aaggacacga tcgtgaagag ttgtcccacg gtggacctga
    12421 tgttgccaat gtccgggaac atcatcgcca gctcctacgc ttacgccttc caactgaagg
    12481 acggctcttt ctacacggca gaagtcaaga acaacataga cttcaagaat ccaatccacg
    12541 agtccttctc gaagtcaggg cccatgttca cccacagacg tgtcgaggag actctcacca
    12601 aggagaacct tgccatagtg gagtaccagc aggttttcaa cagcgcccca agagacatgt
    12661 agatatgaag atgaagatga aatatttggt gtgtcaaata aaaagcttgt gtgcttaagt
    12721 ttgtgttttt ttcttggctt gttgtgttat gaatttgtgg ctttttctaa tattaaatga
    12781 atgtaagatc tcattataat gaataaacaa atgtttctat aatccattgt gaatgttttg
    12841 ttggatctct tctgcagcat ataactactg tatgtgctat ggtatggact atggaatatg
    12901 attaaagata agatgggctc atagagtaaa acgaggcgag ggacctataa acctcccttc
    12961 atcatgctat ttcatgatct attttataaa ataaagatgt agaaaaaagt aagcgtaata
    13021 accgcaaaac aaatgattta aaacatggca cataatgagg agattaagtt cggtttacgt
    13081 ttattttagt actaattgta acgtgagact acgtatcggg aatcgcctaa ttaaagcatt
    13141 aatgcgaacc tgattagatt caccgaccct cctatcgtgt cgacctttct gtttcttaga
    13201 attttttggt agtctatgta ctaataatgt cagcttcgta tttatttcat aagcaatttg
    13261 catttgcaat ttgtttttta cttttatttt tattgtattg tggaatgtgg actcgtacca
    13321 acatgaagtt atataccacc aaaaaaatta cagttagtca aaagattcac gagtgagagc
    13381 tacttatgat tgtcttttac gtatatgtct aattgtctat ttgctcaata atctttgtac
    13441 tttcttttgt cgttgataaa atcacaaagt tccaaaagta atcgaatgat ttgcttttaa
    13501 gaaaagaaga gctcaataat tcaacatata tctgtacaca attaccgcct ttgagtgagc
    13561 tctagatgcc acctgacgtc taagaagaat tcgtaatcat gtcatagctg tttcctgtgt
    13621 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
    13681 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
    13741 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
    13801 gcggtttgcg tattggctag agcagcttgc caacatggtg gagcacgaca ctctcgtcta
    13861 ctccaagaat atcaaagata cagtctcaga agaccaaagg gctattgaga cttttcaaca
    13921 aagggtaata tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcaa
    13981 aaggacagta gaaaaggaag gtggcaccta caaatgccat cattgcgata aaggaaaggc
    14041 tatcgttcaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag
    14101 catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgaaca
    14161 tggtggagca cgacactctc gtctactcca agaatatcaa agatacagtc tcagaagacc
    14221 aaagggctat tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt
    14281 gcccagctat ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat
    14341 gccatcattg cgataaagga
//