Lenti-mEGFP-drG3BP2

LOCUS       Lenti-mEGFP-drG3BP2           10453 bp DNA     circular SYN 10-DEC-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10453)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 29, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..10453
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2210..2513
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2514..2717
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      2756..2765
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2762..3478
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             3497..5020
                     /codon_start=1
                     /label=drG3BP2
                     /translation="MVMEKPSPLLVGREFVRQYYTLLNKAPDFLHRFYGRNSSYVHGGL
                     DLNGKLSEAVYGQAEIHKKVMSLQFSECHTKIRHVDAHATLSDGVVVQVMGELSNNGQP
                     MRKFLQTFVLAPEGSVANKFYVHNDIFRYEDEVFGDSEAELDEESEEETDEPEDRQPSP
                     EPLQDSPSNANCYEPHPVSCNNGVEEAHEEAVMDLQSEVAPDPKIEESKLKVEEKDVEE
                     FEEKALSPVPLESPPNIQEPPKTSSWASVTSKNLPLSGTVPSAGGQPHVVKAPNTQPRV
                     ETKPEMLSASIRPRDQRIRDRPPVTSRGPRSDGVPSSEPQAGKPHFSFVNKGRADESSE
                     MDSRRVVRYPDSHQLFVGNLPHDIDESELKDFFMTFGNVVELRINTKSTGGKIPNFGFV
                     VFDDSEPVQRILGVKPIMFRGEVRLNVEEKKTRAMRERETRSGVGDDRRDVRRSDRGPG
                     GPRGIAGGNMMRERDGRGPPSRVATVPKPGLASGRGSGSNEGRFNAQRR"
     promoter        5031..5530
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             5557..6351
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    6375..6963
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(6846..6857)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7171..7804
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7933..7949)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7957..7973
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7981..8011)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8026..8047
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(8335..8920)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9091..9951)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9952..10056)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10104..10238
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggc gttacataac
     2221 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     2281 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     2341 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     2401 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     2461 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
     2521 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
     2581 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
     2641 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
     2701 tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac cggtcgccac
     2761 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     2821 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     2881 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     2941 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     3001 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     3061 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     3121 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     3181 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     3241 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     3301 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     3361 ctacctgagc acccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt
     3421 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
     3481 ctcagatctc gagctcatgg ttatggaaaa gccgagcccg ctgctggtgg gtcgtgaatt
     3541 tgtgcgccag tattataccc tgctgaataa ggcaccggat tttctgcatc gtttttatgg
     3601 ccgcaatagt agctatgtgc atggcggcct ggatctgaat ggcaaactga gtgaagcagt
     3661 ttatggccag gcagaaattc ataaaaaagt gatgagcctg cagtttagtg aatgtcatac
     3721 caaaattcgc catgtggatg cacatgcaac cctgagcgat ggcgttgttg ttcaggttat
     3781 gggtgaactg agcaataatg gtcagccgat gcgtaaattt ctgcagacct ttgttctggc
     3841 cccggaaggc agcgttgcca ataagtttta tgttcataat gacatcttcc gctatgaaga
     3901 tgaagtgttt ggcgatagcg aagccgaact ggatgaagaa agtgaagaag aaaccgatga
     3961 accggaagat cgtcagccga gcccggaacc gctgcaggat agcccgagca atgccaattg
     4021 ttatgaaccg catccggtga gttgcaataa tggcgtggaa gaagcacatg aagaagcagt
     4081 gatggatctg cagagtgaag tggcaccgga ccctaaaatt gaagaaagta aactgaaagt
     4141 ggaggaaaaa gatgtggaag aatttgaaga aaaggccctg agtccggtgc cgctggaaag
     4201 cccgccgaat attcaggaac cgccgaaaac cagtagctgg gccagtgtga ccagcaaaaa
     4261 tctgccgctg agcggcaccg tgccgagtgc aggtggccag cctcatgttg ttaaagcccc
     4321 gaatacccag ccgcgtgtgg aaaccaaacc ggaaatgctg agcgcaagta ttcgtccgcg
     4381 tgatcagcgt attcgtgatc gtccgccggt gaccagtcgc ggccctagaa gtgatggcgt
     4441 gccgagtagt gaaccgcagg caggcaaacc gcattttagt tttgtgaata agggtcgcgc
     4501 agatgaaagc agtgaaatgg atagtcgtcg tgtggtgcgc tatccggata gtcatcagct
     4561 gtttgtgggc aatctgccgc atgatattga tgaaagtgaa ctgaaagatt tcttcatgac
     4621 ctttggtaat gttgttgaac tgcgtattaa taccaaaagc accggtggca aaattccgaa
     4681 ttttggtttt gttgtgttcg atgatagtga accggttcag cgtattctgg gcgttaaacc
     4741 gattatgttt cgtggtgaag ttcgcctgaa tgtggaagaa aagaaaaccc gtgccatgcg
     4801 cgaacgcgaa acccgcagcg gtgtgggcga tgatcgccgt gatgttcgtc gcagtgatcg
     4861 cggtccgggc ggccctagag gcattgcagg cggtaatatg atgcgtgaac gcgatggccg
     4921 cggtccgccg agtcgtgttg ccacagtgcc gaaaccgggt ctggcaagcg gtcgcggcag
     4981 cggtagcaat gaaggccgtt ttaatgccca gcgtcgctaa gaattctacc gggtagggga
     5041 ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg
     5101 ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc
     5161 tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc
     5221 ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag
     5281 tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg
     5341 ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg
     5401 gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc
     5461 ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc
     5521 gggcctttcg acctgcagcc caagcttcct aggaccatga ttgaacaaga tggattgcac
     5581 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     5641 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     5701 gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg
     5761 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     5821 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     5881 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     5941 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     6001 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     6061 gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat
     6121 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     6181 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     6241 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     6301 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg acccgggcgg
     6361 ggcgcgtctg gaacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     6421 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     6481 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     6541 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     6601 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     6661 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     6721 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     6781 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     6841 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     6901 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     6961 cgcctggaat taattctgca gtcgagacct agaaaaacat ggagcaatca caagtagcaa
     7021 tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg
     7081 tttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     7141 ttagccactt tttaaaagaa aagaggggac tggaagggct aattcactcc caacgaagac
     7201 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact
     7261 acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac
     7321 cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc
     7381 ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca
     7441 gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct
     7501 gatatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg
     7561 gcgggactgg ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt
     7621 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     7681 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     7741 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     7801 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     7861 tcagagagtg agaggccttg acattgctag cgttttaccg tcgacctcta gctagagctt
     7921 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     7981 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     8041 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     8101 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     8161 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     8221 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     8281 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     8341 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     8401 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     8461 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     8521 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     8581 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     8641 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     8701 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     8761 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     8821 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtttttttgt
     8881 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     8941 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     9001 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     9061 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     9121 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     9181 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     9241 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     9301 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     9361 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     9421 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     9481 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     9541 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     9601 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     9661 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     9721 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     9781 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     9841 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     9901 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     9961 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
    10021 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
    10081 tgacgtcgac ggatcgggag atcaacttgt ttattgcagc ttataatggt tacaaataaa
    10141 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
    10201 tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc
    10261 aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac ctagtggttt
    10321 catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt
    10381 taaatatgta ctacaaactt agtagttttt aaagaaattg tatttgttaa atatgtacta
    10441 caaacttagt agt
//