| 质粒名称: | gag/pol |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 14887 |
| 存储实验室: | Tannishtha Reya |
|
载体骨架基本信息 |
|
| 载体名称: | -- |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，逆转录病毒 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | gag / pol |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | n/a |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | gag/pol was a gift from Tannishtha Reya (Addgene plasmid # 14887 ; http://n2t.net/addgene:14887 ; RRID:Addgene_14887) |
| 参考文献部分: | A role for Wnt signalling in self-renewal of haematopoietic stem cells. Reya T, Duncan AW, Ailles L, Domen J, Scherer DC, Willert K, Hintz L, Nusse R, Weissman IL. Nature. 2003 May 22. 423(6938):409-14. 10.1038/nature01593 PubMed 12717450 |
买家导航
gag/pol载体质粒基本信息
gag/pol质粒图谱载体图谱和gag/pol载体序列质粒序列多克隆位点信息
gag/pol质粒载体简介
gag/pol质粒序列载体序列
LOCUS gag/pol 8922 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS gag/pol
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8922)
AUTHORS Reya T, Duncan AW, Ailles L, Domen J, Scherer DC, Willert K, Hintz
L, Nusse R, Weissman IL
TITLE A role for Wnt signalling in self-renewal of haematopoietic stem
cells.
JOURNAL Nature. 2003 May 22. 423(6938):409-14.
PUBMED 12717450
REFERENCE 2 (bases 1 to 8922)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8922
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 55..76
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 91..121
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 129..145
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 134..156
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 153..169
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 153..169
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
polyA_signal 280..414
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(317..336)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 371..390
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
CDS complement(429..5645)
/codon_start=1
/gene="gag-pol"
/product="Moloney murine leukemia virus Gag-Pol
polyprotein"
/label=gag-pol
/note="contains a read-through stop codon"
/translation="MGQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWPT
FNVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVHPK
PPPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGAKPKPQVLSDSGGPLIDLLTEDPP
PYRDPRPPPSDRDGNGGEATPAGEAPDPSPMASRLRGRREPPVADSTTSQAFPLRAGGN
GQLQYWPFSSSDLYNWKNNNPSFSEDPGKLTALIESVLITHQPTWDDCQQLLGTLLTGE
EKQRVLLEARKAVRGDDGRPTQLPNEVDAAFPLERPDWDYTTQAGRNHLVHYRQLLLAG
LQNAGRSPTNLAKVKGITQGPNESPSAFLERLKEAYRRYTPYDPEDPGQETNVSMSFIW
QSAPDIGRKLERLEDLKNKTLGDLVREAEKIFNKRETPEEREERIRRETEEKEERRRTE
DEQKEKERDRRRHREMSKLLATVVSGQKQDRQGGERRRSQLDRDQCAYCKEKGHWAKDC
PKKPRGPRGPRPQTSLLTLDD*GGQGQEPPPEPRITLKVGGQPVTFLVDTGAQHSVLTQ
NPGPLSDKSAWVQGATGGKRYRWTTDRKVHLATGKVTHSFLHVPDCPYPLLGRDLLTKL
KAQIHFEGSGAQVMGPMGQPLQVLTLNIEDEYRLHETSKEPDVSLGSTWLSDFPQAWAE
TGGMGLAVRQAPLIIPLKATSTPVSIKQYPMSQEARLGIKPHIQRLLDQGILVPCQSPW
NTPLLPVKKPGTNDYRPVQDLREVNKRVEDIHPTVPNPYNLLSGLPPSHQWYTVLDLKD
AFFCLRLHPTSQPLFAFEWRDPEMGISGQLTWTRLPQGFKNSPTLFDEALHRDLADFRI
QHPDLILLQYVDDLLLAATSELDCQQGTRALLQTLGNLGYRASAKKAQICQKQVKYLGY
LLKEGQRWLTEARKETVMGQPTPKTPRQLREFLGTAGFCRLWIPGFAEMAAPLYPLTKT
GTLFNWGPDQQKAYQEIKQALLTAPALGLPDLTKPFELFVDEKQGYAKGVLTQKLGPWR
RPVAYLSKKLDPVAAGWPPCLRMVAAIAVLTKDAGKLTMGQPLVILAPHAVEALVKQPP
DRWLSNARMTHYQALLLDTDRVQFGPVVALNPATLLPLPEEGLQHNCLDILAEAHGTRP
DLTDQPLPDADHTWYTDGSSLLQEGQRKAGAAVTTETEVIWAKALPAGTSAQRAELIAL
TQALKMAEGKKLNVYTDSRYAFATAHIHGEIYRRRGLLTSEGKEIKNKDEILALLKALF
LPKRLSIIHCPGHQKGHSAEARGNRMADQAARKAAITETPDTSTLLIENSSPYTSEHFH
YTVTDIKDLTKLGAIYDKTKKYWVYQGKPVMPDQFTFELLDFLHQLTHLSFSKMKALLE
RSHSPYYMLNRDRTLKNITETCKACAQVNASKSAVKQGTRVRGHRPGTHWEIDFTEIKP
GLYGYKYLLVFIDTFSGWIEAFPTKKETAKVVTKKLLEEIFPRFGMPQVLGTDNGPAFV
SKVSQTVADLLGIDWKLHCAYRPQSSGQVERMNRTIKETLTKLTLATGSRDWVLLLPLA
LYRARNTPGPHGLTPYEILYGAPPPLVNFPDPDMTRVTNSPSLQAHLQALYLVQHEVWR
PLAAAYQEQLDRPVVPHPYRVGDTVWVRRHQTKNLEPRWKGPYTVLLTTPTALKVDGIA
AWIHAAHVKAADPGGGPSSRLTWRVQRSQNPLKIRLTREAP"
misc_feature 492..866
/label=pol region
/note="Moloney murine leukemia virus (MMLV) pol region
containing the splice acceptor site"
primer_bind complement(538..554)
/label=pBMN 5'
/note="MMLV sequence, for inserts in pBMN retroviral
vector"
primer_bind complement(5242..5258)
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
primer_bind complement(5274..5296)
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
regulatory 5642..5651
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
intron 5712..5808
/label=SV40 intron
/note="modified SV40 intron with splice donor and acceptor
sites"
promoter complement(5854..6115)
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
primer_bind complement(6475..6492)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(6475..6491)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6484..6506)
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 6819..6841
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(6879..6897)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 6965..7069
/gene="bla"
/label=AmpR promoter
CDS 7070..7930
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(7288..7307)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 8101..8689
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8590..8609
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 8843..8860
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
61 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
121 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
181 caagcttgca tgcctgcagg tcgactctag aggatccgaa aaaacctccc acacctcccc
241 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
301 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
361 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccccgcg
421 gccgctcatt agggggcctc gcgggttaac cttattttta aggggttttg agagcgttga
481 acgcgccatg tcagtctaga ggatggtcca cccccggggt cggcagcctt cacgtgggcg
541 gcgtgtatcc aagctgcgat gccgtctact ttgagggcgg tgggggtggt cagcaggact
601 gtgtaaggtc ctttccagcg aggttctagg ttcttagtct ggtgtcggcg gacccacact
661 gtgtcgccga ctcggtaagg gtgaggtacc accggtcggt ccagttgttc ttggtaggct
721 gccgccagag gtctccagac ttcgtgctgg actaagtaga gagcctgtaa gtgagcttgg
781 agagaggggc tgttagtaac tcttgtcatg tcagggtcag ggaagtttac aaggggcggg
841 ggtgccccat ataagatctc atatggggtg aggccatggg ggcccggcgt gttgcgggct
901 cggtacaggg ctaaggggag taggagcacc cagtctctag agccagttgc aagcgttaat
961 ttagttaaag tctccttgat ggttctattc attctttcta cctggcctga gctttggggt
1021 ctgtatgcac aatgtaattt ccaatcaatc cccaacagat cggccactgt ctgactcacc
1081 ttggagacga aggcaggccc attgtcagtt cccaatacct gaggcatgcc gaacctgggg
1141 aagatctcct ctagtagctt cttggttacg accttggcgg tttctttctt ggttgggaag
1201 gcttctatcc agccagaaaa ggtatctata aaaactagaa gatatttata gccatacaat
1261 ccgggcttta tctcggtgaa atcgatctcc caatgagtgc cgggccgatg cccgcggacc
1321 ctagttccct gtttaacggc agacttgctg gcgttgactt gtgcacaagc tttgcaggtc
1381 tcagtgatat ttttgagtgt tcgatcccgg ttcagcatgt agtagggact gtggcttctc
1441 tctaggagag ccttcatttt tgagaagctg aggtgagtca gctgatgaag aaagtctaat
1501 aattcaaaag taaactggtc aggcatcaca ggttttcctt ggtagaccca atacttcttt
1561 gttttatcat aaatggcccc caacttggtt aggtccttta tatcagtcac tgtgtaatga
1621 aaatgttctg aggtgtaggg tgatgaattt tctatgagga gggtagaggt gtctggagtc
1681 tctgtgatgg ctgcctttcg ggccgcttgg tcagccatcc ggttgcctct agcctcggcg
1741 ctgtgtccct tttgatgtcc tggacaatgg attatgctaa gtcttttggg cagaaagagg
1801 gcttttagta gggccaagat ctcgtcttta tttttgatct ctttgccttc tgatgtgagc
1861 aacccacgcc ttctgtatat ttctccatgg atatgggcag tagcaaaagc ataacggcta
1921 tcagtataaa catttagctt cttaccttct gccatcttta gggcctgggt gagtgctatc
1981 agttcagccc gctgagcgga tgtcccggct ggcagggctt tagcccagat tacctcggtc
2041 tcggtggtca ccgcagctcc cgccttacgc tgtccctctt gtaagagact gcttccatcc
2101 gtgtaccagg tgtggtcggc gtctgggagc ggctggtccg ttaggtcggg tcgggttccg
2161 tgggcttcgg ccaggatatc aaggcagttg tgttgcagcc cttcctcagg cagtgggagc
2221 agcgtagccg ggttcagggc taccaccggt ccgaactgga cccggtccgt gtccaaaagc
2281 aaggcctgat agtgagtcat ccgggcgttg gaaagccagc ggtcgggggg ttgtttgact
2341 agtgcctcta ctgcatgggg ggccagaatg actagtggct gtcccatggt tagcttgcct
2401 gcatcctttg tcagtacggc aatggctgct accatccgta ggcaaggggg ccacccagct
2461 gctactgggt ctagcttttt ggacaggtag gccaccggcc gacgccaagg tcccagtttt
2521 tgcgttagga cacctttggc gtagccctgc ttctcgtcga caaagagttc aaagggctta
2581 gtcaaatctg gcaaccccag ggctggggca gttagaagag cttgcttgat ttcttgatag
2641 gccttttgtt ggtctgggcc ccaattaaac agagtccccg ttttggtgag agggtacaag
2701 ggggctgcca tttctgcaaa cccagggatc cagaggcgac agaagcctgc cgtccctagg
2761 aactccctta gttgtcgagg ggtcttcgga gtaggctgcc ccatcacagt ctcttttctg
2821 gcctcagtca gccatctctg accctctttt agaagatacc ccagatactt gacctgtttc
2881 tggcaaattt gggctttctt ggccgaggcc cgatacccga ggttccctag ggtttgtaac
2941 agggcccgag taccttgttg gcagtctagc tcagaagtgg cggccagcag taagtcatcc
3001 acgtactgta gcaggatcaa gtctgggtgc tggatccgga agtctgctag gtctctgtgc
3061 agtgcctcat caaacagggt gggactgttt ttgaaaccct gtgggagtct ggtccaggtc
3121 aattgtcctg agattcccat ctctggatct ctccactcaa aggcgaagag aggctgactg
3181 gtggggtgga gtctcaggca gaaaaaggca tcctttaaat caagcacagt gtaccactgg
3241 tgggacggtg ggagcccgct caagaggttg taagggttgg gcacggtggg gtggatgtct
3301 tccacccgct tgttgacttc tctcagatcc tggacaggcc tataatcatt agtccctggt
3361 ttcttaacgg gtagcagggg cgtgttccag ggggactggc agggtaccag tattccctgg
3421 tccaacagtc tctgtatgtg gggcttgatc cccagtctgg cttcttgtga catggggtat
3481 tgttttatgg acacgggggt agaggttgct ttcagaggta tgatcagagg agcttggcga
3541 actgccagtc ccatgccccc ggtttccgcc caggcctgag gaaaatcaga cagccatgtg
3601 gaccctagag aaacatctgg ctcttttgag gtctcatgta gccgatactc atcttctata
3661 tttagggtca acacttgcag gggctgcccc attggtccca taacctgagc tcctgatccc
3721 tcaaagtgga tttgggcttt tagtttagtc agcaaatctc ttcctaacag aggataggga
3781 cagtctggta catggaggaa agagtgggtg accttaccgg tagctagatg tactttgcga
3841 tccgtggtcc agcgataccg ctttcctcca gtagcccctt ggacccaggc agacttatca
3901 cttaggggtc caggattttg ggtcagcacg gagtgttggg ccccagtatc taccaggaag
3961 gtgacgggtt gccccccgac tttgagggtt atcctgggtt cagggggggg ctcctgaccc
4021 tgacctccct agtcatctag ggtcaggagg gaggtctggg gtcttggtcc ccgaggtcct
4081 cgtggtttct tgggacaatc tttagcccag tgcccctttt ctttgcagta ggcacactgg
4141 tcgcgatcga gttgggacct ccttcgttct cctccctgtc tatcctgttt ctgtccacta
4201 acgacagtgg ccaatagctt gctcatctct ctatgtctcc tacgatctct ttctttctct
4261 ttctgctcat cctctgtcct acggcgttct tctttttcct ctgtttctct cctgatacgt
4321 tcctctcttt cttccggggt ttctcgttta ttaaagatct tttctgcctc tctaaccaaa
4381 tctccaagcg tcttgttttt taaatcttct aacctctcta actttctccc aatgtctggg
4441 gcagactgcc aaatgaaaga catagacaca ttagtttctt gccctgggtc ctcagggtca
4501 taaggagtgt acctgcgata ggcttcctta agtctctcta ggaaggccga gggagactca
4561 ttgggccctt gtgttattcc ttttaccttg gccaaattgg tggggcttct gcccgcgttt
4621 tggagacccg ctaggagcaa ctggcgatag tggactaggt ggttcctacc tgcctgggtg
4681 gtgtaatccc agtctgggcg ctcgagggga aaagcggcat cgacttcatt gggcagttga
4741 gtggggcgcc catcatcgcc ccgcaccgcc tttctagcct ctaagagcac ccgttgtttt
4801 tcttctccgg tcagcagagt ccccaacagc tgctgacagt cgtcccaggt gggctgatgg
4861 gtgatgagaa cagactcgat cagagctgtc agtttacctg gatcttcaga aaaagaaggg
4921 ttattatttt tccagttgta aaggtcagaa gaggagaacg gccagtattg aagctgtccg
4981 tttcctcctg cgcggagggg gaatgcctgc gaggtagtgg agtcggccac agggggctcc
5041 cgtctcccac gtaggcgaga tgccattggg gaggggtccg gtgcctctcc cgcaggggtc
5101 gcttctccac catttccgtc cctgtcggaa gggggtggtc ttgggtccct ataaggcggg
5161 gggtcttctg taagtaggtc gatgagcggc cccccactgt cagaaagaac ttgaggttta
5221 ggtttggcgc ctagagaagg agtgagggct ggataaaggg aggatcgagg cggggtcgaa
5281 cgaggaggtt caagggggag agacggggcg gatggaggaa gaggaggcgg aggcttaggg
5341 tgtacaaagg gcttgaccca gggagggggg tcaaaagcca aggcttccca ggtcacgatg
5401 taggggacct ggtctgggtg tccatgcggg ccaggtgaaa agaccttgat cttaacctgg
5461 gtgatgaggt ctcggttaaa ggtgccgtct cgcggccatc cgacgttaaa ggttggccat
5521 tctgcagagc agaaggtaac ccaacgtctc ttcttgacat ctaccgactg gttgtgagcg
5581 atccgctcga catctttcca gtgacctaag gtcaaactta agggagtggt aacagtctgg
5641 cccatggtgg cggcgcggcc gcgggtacaa ttccgcagct tttagagcag aagtaacact
5701 tccgtacagg cctagaagta aaggcaacgt ccactgagga gcagttcttt gatttgcacc
5761 accaccggat ccgggacctg aaataaaaga caaaaagact aaacttacca gttaactttc
5821 tggtttttca gttcctcgag tacccagctt ggaggtgcac accaatgtgg tgaatggtca
5881 aatggcgttt attgtatcga gctaggcact taaatacaat atctctgcaa tgcggaattc
5941 agtggttcgt ccaatccatg tcagacccgt ctgttgcctt cctaataagg cacgatcgta
6001 ccaccttact tccaccaatc ggcatgcacg gtgctttttc tctccttgta aggcatgttg
6061 ctaactcatc gttaccatgt tgcaagacta caagagtatt gcataagact acatttcccc
6121 ctccctatgc aaaagcgaaa ctactatatc ctgaggggac tcctaaccgc gtacaaccga
6181 agccccgctt ttcgcctaaa cacaccctag tcccctcaga tacgcgtata tctggcccgt
6241 acatcgcgaa gcagcgcaaa acgcctaacc ctaagcagat tcttcatgca attgtcggtc
6301 aagccttgcc ttgttgtagc ttaaattttg ctcgcgcact actcagcgac ctccaacaca
6361 caagcaggga gcagatactg gcttaactat gcggcatcag agcagattgt actgagagtg
6421 caccatacgg atctgcgatg ataagctgtc aaacatgaga attggtcgaa attcactggc
6481 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
6541 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
6601 ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca
6661 tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc
6721 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
6781 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
6841 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
6901 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
6961 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
7021 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
7081 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
7141 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
7201 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
7261 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
7321 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
7381 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
7441 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
7501 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
7561 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
7621 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
7681 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
7741 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
7801 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
7861 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
7921 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
7981 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
8041 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
8101 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
8161 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
8221 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
8281 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
8341 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
8401 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
8461 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
8521 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
8581 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
8641 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
8701 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
8761 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
8821 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
8881 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tg
//
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gag/pol质粒载体应用举例
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