| 质粒名称: | SQT1659,pSQT1659-AsCpf1-NLS-3×HA |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 78743 |
| 存储实验室: | Keith Joung |
|
载体骨架基本信息 |
|
| 载体名称: | pCAG-GFP |
| 载体出品公司: | Cepko lab (Addgene plasmid# 11179) |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达,CRISPR |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | mammalian codon-optimized Acidaminococcus sp. BV3L6 Cpf1-NLS-3xHA,Cpf1 |
| 物种来源: | Acidaminococcus sp. BV3L6 |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CAG |
| 标签/融合蛋白: |
NLS（插接件上的C端子）
3x HA（插入件上的C端子）
|
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | pCAG-F (5'-GCAACGTGCTGGTTATTGTG-3') |
| 3' 测序引物 | M13-rev |
|
文献引用方法 |
|
| 材料和方法部分: | SQT1659 was a gift from Keith Joung (Addgene plasmid # 78743 ; http://n2t.net/addgene:78743 ; RRID:Addgene_78743) |
| 参考文献部分: | Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human cells. Kleinstiver BP, Tsai SQ, Prew MS, Nguyen NT, Welch MM, Lopez JM, McCaw ZR, Aryee MJ, Joung JK. Nat Biotechnol. 2016 Jun 27. doi: 10.1038/nbt.3620. 10.1038/nbt.3620 PubMed 27347757 |
买家导航
SQT1659载体质粒基本信息
SQT1659质粒图谱载体图谱和SQT1659载体序列质粒序列多克隆位点信息
SQT1659质粒载体简介
SQT1659质粒序列载体序列
LOCUS SQT1659 8899 bp ds-DNA circular SYN 19-APR-2019
DEFINITION Human expression plasmid for AsCpf1-NLS-3xHA.
ACCESSION .
VERSION .
KEYWORDS SQT1659
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8899)
AUTHORS Kleinstiver BP, Tsai SQ, Prew MS, Nguyen NT, Welch MM, Lopez JM,
McCaw ZR, Aryee MJ, Joung JK
TITLE Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human
cells.
JOURNAL Nat Biotechnol. 2016 Jun 27. doi: 10.1038/nbt.3620.
PUBMED 27347757
REFERENCE 2 (bases 1 to 8899)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 19, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8899
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 1..380
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
intron 657..1673
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
primer_bind 1597..1619
/label=pCAGGS-5
/note="Chimeric intron in CAG promoter, forward primer"
primer_bind 1681..1700
/label=pCAG-F
/note="Rabbit beta-globin intron, for pCAG plasmids,
forward primer"
CDS 1772..5692
/codon_start=1
/product="CRISPR-associated protein from Acidaminococcus
sp. BV3L6 (Zetsche et al., 2015)"
/label=AsCpf1
/translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARND
HYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRN
AIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFD
KFTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHF
ENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVL
NLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLL
RNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTG
KITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTL
KKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKA
RNYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYK
ALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEP
LEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSID
LSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKG
HHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLK
DQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFF
HVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQR
SLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAV
VVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQ
FTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYD
VKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIEN
HRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQM
RNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKES
KDLKLQNGISNQDWLAYIQELRN"
regulatory 5412..5421
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 5693..5740
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/label=nucleoplasmin NLS
/translation="KRPAATKKAGQAKKKK"
CDS 5747..5827
/codon_start=1
/product="three tandem HA epitope tags"
/label=3xHA
/translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
primer_bind complement(5917..5936)
/label=Bglob-pA-R
/note="Rabbit beta-globin polyA region, reverse primer"
polyA_site 5982..6037
/label=-beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal"
primer_bind complement(6036..6055)
/label=rbglobpA-R
/note="Rabbit beta-globin polyA, reverse primer. Also
called rb-glob-pA-term-R"
primer_bind complement(6398..6414)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6398..6414)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(6411..6433)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 6422..6438
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6446..6476)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6491..6512
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 6571..6766
/label=SV40 promoter
/note="SV40 early promoter"
rep_origin 6617..6752
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 6679..6698
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
polyA_signal 6772..6906
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(6809..6828)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 6863..6882
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(6974..6991)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(7145..7733)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7225..7244)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7904..8764)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8527..8546
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8765..8869)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
481 tgggggcggg gggggggggn cgcgcgccag gcggggcggg gcggggcgag gggcggggcg
541 gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt
601 ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag
661 tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc
721 ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg
781 gctgtaatta gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc
841 ttgaggggct ccgggagggc cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt
901 gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg
961 ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg
1021 gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
1081 tgggggggtg agcagggggt gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc
1141 ctccccgagt tgctgagcac ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg
1201 cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc
1261 cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg cccccggagc gccggcggct
1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
1381 gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct
1441 ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc
1501 ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca gcctcggggc tgtccgcggg
1561 gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg
1621 gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg
1681 gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattctgc agtcgacggt
1741 accgcgggcc cgggatccac cggtcgccac catgacacag ttcgagggct ttaccaacct
1801 gtatcaggtg agcaagacac tgcggtttga gctgatccca cagggcaaga ccctgaagca
1861 catccaggag cagggcttca tcgaggagga caaggcccgc aatgatcact acaaggagct
1921 gaagcccatc atcgatcgga tctacaagac ctatgccgac cagtgcctgc agctggtgca
1981 gctggattgg gagaacctga gcgccgccat cgactcctat agaaaggaga aaaccgagga
2041 gacaaggaac gccctgatcg aggagcaggc cacatatcgc aatgccatcc acgactactt
2101 catcggccgg acagacaacc tgaccgatgc catcaataag agacacgccg agatctacaa
2161 gggcctgttc aaggccgagc tgtttaatgg caaggtgctg aagcagctgg gcaccgtgac
2221 cacaaccgag cacgagaacg ccctgctgcg gagcttcgac aagtttacaa cctacttctc
2281 cggcttttat gagaacagga agaacgtgtt cagcgccgag gatatcagca cagccatccc
2341 acaccgcatc gtgcaggaca acttccccaa gtttaaggag aattgtcaca tcttcacacg
2401 cctgatcacc gccgtgccca gcctgcggga gcactttgag aacgtgaaga aggccatcgg
2461 catcttcgtg agcacctcca tcgaggaggt gttttccttc cctttttata accagctgct
2521 gacacagacc cagatcgacc tgtataacca gctgctggga ggaatctctc gggaggcagg
2581 caccgagaag atcaagggcc tgaacgaggt gctgaatctg gccatccaga agaatgatga
2641 gacagcccac atcatcgcct ccctgccaca cagattcatc cccctgttta agcagatcct
2701 gtccgatagg aacaccctgt ctttcatcct ggaggagttt aagagcgacg aggaagtgat
2761 ccagtccttc tgcaagtaca agacactgct gagaaacgag aacgtgctgg agacagccga
2821 ggccctgttt aacgagctga acagcatcga cctgacacac atcttcatca gccacaagaa
2881 gctggagaca atcagcagcg ccctgtgcga ccactgggat acactgagga atgccctgta
2941 tgagcggaga atctccgagc tgacaggcaa gatcaccaag tctgccaagg agaaggtgca
3001 gcgcagcctg aagcacgagg atatcaacct gcaggagatc atctctgccg caggcaagga
3061 gctgagcgag gccttcaagc agaaaaccag cgagatcctg tcccacgcac acgccgccct
3121 ggatcagcca ctgcctacaa ccctgaagaa gcaggaggag aaggagatcc tgaagtctca
3181 gctggacagc ctgctgggcc tgtaccacct gctggactgg tttgccgtgg atgagtccaa
3241 cgaggtggac cccgagttct ctgcccggct gaccggcatc aagctggaga tggagccttc
3301 tctgagcttc tacaacaagg ccagaaatta tgccaccaag aagccctact ccgtggagaa
3361 gttcaagctg aactttcaga tgcctacact ggcctctggc tgggacgtga ataaggagaa
3421 gaacaatggc gccatcctgt ttgtgaagaa cggcctgtac tatctgggca tcatgccaaa
3481 gcagaagggc aggtataagg ccctgagctt cgagcccaca gagaaaacca gcgagggctt
3541 tgataagatg tactatgact acttccctga tgccgccaag atgatcccaa agtgcagcac
3601 ccagctgaag gccgtgacag cccactttca gacccacaca acccccatcc tgctgtccaa
3661 caatttcatc gagcctctgg agatcacaaa ggagatctac gacctgaaca atcctgagaa
3721 ggagccaaag aagtttcaga cagcctacgc caagaaaacc ggcgaccaga agggctacag
3781 agaggccctg tgcaagtgga tcgacttcac aagggatttt ctgtccaagt ataccaagac
3841 aacctctatc gatctgtcta gcctgcggcc atcctctcag tataaggacc tgggcgagta
3901 ctatgccgag ctgaatcccc tgctgtacca catcagcttc cagagaatcg ccgagaagga
3961 gatcatggat gccgtggaga caggcaagct gtacctgttc cagatctata acaaggactt
4021 tgccaagggc caccacggca agcctaatct gcacacactg tattggaccg gcctgttttc
4081 tccagagaac ctggccaaga caagcatcaa gctgaatggc caggccgagc tgttctaccg
4141 ccctaagtcc aggatgaaga ggatggcaca ccggctggga gagaagatgc tgaacaagaa
4201 gctgaaggat cagaaaaccc caatccccga caccctgtac caggagctgt acgactatgt
4261 gaatcacaga ctgtcccacg acctgtctga tgaggccagg gccctgctgc ccaacgtgat
4321 caccaaggag gtgtctcacg agatcatcaa ggataggcgc tttaccagcg acaagttctt
4381 tttccacgtg cctatcacac tgaactatca ggccgccaat tccccatcta agttcaacca
4441 gagggtgaat gcctacctga aggagcaccc cgagacacct atcatcggca tcgatcgggg
4501 cgagagaaac ctgatctata tcacagtgat cgactccacc ggcaagatcc tggagcagcg
4561 gagcctgaac accatccagc agtttgatta ccagaagaag ctggacaaca gggagaagga
4621 gagggtggca gcaaggcagg cctggtctgt ggtgggcaca atcaaggatc tgaagcaggg
4681 ctatctgagc caggtcatcc acgagatcgt ggacctgatg atccactacc aggccgtggt
4741 ggtgctggag aacctgaatt tcggctttaa gagcaagagg accggcatcg ccgagaaggc
4801 cgtgtaccag cagttcgaga agatgctgat cgataagctg aattgcctgg tgctgaagga
4861 ctatccagca gagaaagtgg gaggcgtgct gaacccatac cagctgacag accagttcac
4921 ctcctttgcc aagatgggca cccagtctgg cttcctgttt tacgtgcctg ccccatatac
4981 atctaagatc gatcccctga ccggcttcgt ggaccccttc gtgtggaaaa ccatcaagaa
5041 tcacgagagc cgcaagcact tcctggaggg cttcgacttt ctgcactacg acgtgaaaac
5101 cggcgacttc atcctgcact ttaagatgaa cagaaatctg tccttccaga ggggcctgcc
5161 cggctttatg cctgcatggg atatcgtgtt cgagaagaac gagacacagt ttgacgccaa
5221 gggcacccct ttcatcgccg gcaagagaat cgtgccagtg atcgagaatc acagattcac
5281 cggcagatac cgggacctgt atcctgccaa cgagctgatc gccctgctgg aggagaaggg
5341 catcgtgttc agggatggct ccaacatcct gccaaagctg ctggagaatg acgattctca
5401 cgccatcgac accatggtgg ccctgatccg cagcgtgctg cagatgcgga actccaatgc
5461 cgccacaggc gaggactata tcaacagccc cgtgcgcgat ctgaatggcg tgtgcttcga
5521 ctcccggttt cagaacccag agtggcccat ggacgccgat gccaatggcg cctaccacat
5581 cgccctgaag ggccagctgc tgctgaatca cctgaaggag agcaaggatc tgaagctgca
5641 gaacggcatc tccaatcagg actggctggc ctacatccag gagctgcgca acaaaaggcc
5701 ggcggccacg aaaaaggccg gccaggcaaa aaagaaaaag ggatcctacc catacgatgt
5761 tccagattac gcttatccct acgacgtgcc tgattatgca tacccatatg atgtccccga
5821 ctatgcctaa agcggccgca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg
5881 tgtggccaat gccctggctc acaaatacca ctgagatctt tttccctctg ccaaaaatta
5941 tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt
6001 cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc
6061 aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat
6121 gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga aacagccccc
6181 tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt
6241 ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc
6301 cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc tcttatggag
6361 atccctcgac ctgcagccca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
6421 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
6481 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
6541 agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat tagtcagcaa ccatagtccc
6601 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
6661 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
6721 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc taacttgttt
6781 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
6841 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
6901 tggatccgct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
6961 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
7021 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
7081 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
7141 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
7201 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
7261 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
7321 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
7381 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
7441 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
7501 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
7561 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
7621 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
7681 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
7741 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
7801 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
7861 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
7921 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
7981 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
8041 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
8101 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
8161 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
8221 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
8281 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
8341 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
8401 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
8461 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
8521 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
8581 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
8641 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
8701 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
8761 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
8821 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
8881 gaaaagtgcc acctgggtc
//
