| 质粒名称: | pAAVLINK-EF1alpha-HA-5'-CDC42BPB |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001859 |
| 产品用途: | 5' AAVLINK plasmid for CDC42BPB expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1alpha |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | CDC42BPB |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1alpha-HA-5'-CDC42BPB
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1alpha-HA-5'-CDC42BPB载体质粒基本信息
pAAVLINK-EF1alpha-HA-5'-CDC42BPB质粒图谱载体图谱和pAAVLINK-EF1alpha-HA-5'-CDC42BPB载体序列质粒序列多克隆位点信息
pAAVLINK-EF1alpha-HA-5'-CDC42BPB质粒载体简介
pAAVLINK-EF1alpha-HA-5'-CDC42BPB质粒序列载体序列
LOCUS pAAVLINK-EF1alpha-HA-5'-CDC42BPB 7277 bp DNA circular SYN 24-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7277)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 11, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7277
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
/note="color: #ffe4c4"
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..4417
/codon_start=1
/gene="CDC42BPB"
/gene_synonym="CHOCNS; MRCKB"
/product="serine/threonine-protein kinase MRCK betaisoform
1"
/EC_number="2.7.11.1"
/label=CDC42BPB
/note="isoform 1 is encoded by transcript variant 1;
serine/threonine-protein kinase MRCK beta; myotonic
dystrophy protein kinase-like beta; DMPK-like beta;
myotonic dystrophy kinase-related CDC42-binding kinase
beta; CDC42BP-beta; CDC42 binding protein kinase beta
(DMPK-like)"
/db_xref="GI:115527097"
/db_xref="CCDS:CCDS9978.1"
/db_xref="GeneID:9578"
/db_xref="HGNC:HGNC:1738"
/db_xref="MIM:614062"
/protein_id="NP_006026.3"
/translation="SAKVRLKKLEQLLLDGPWRNESALSVETLLDVLVCLYTECSHSAL
RRDKYVAEFLEWAKPFTQLVKEMQLHREDFEIIKVIGRGAFGEVAVVKMKNTERIYAMK
ILNKWEMLKRAETACFREERDVLVNGDCQWITALHYAFQDENHLYLVMDYYVGGDLLTL
LSKFEDKLPEDMARFYIGEMVLAIDSIHQLHYVHRDIKPDNVLLDVNGHIRLADFGSCL
KMNDDGTVQSSVAVGTPDYISPEILQAMEDGMGKYGPECDWWSLGVCMYEMLYGETPFY
AESLVETYGKIMNHEERFQFPSHVTDVSEEAKDLIQRLICSRERRLGQNGIEDFKKHAF
FEGLNWENIRNLEAPYIPDVSSPSDTSNFDVDDDVLRNTEILPPGSHTGFSGLHLPFIG
FTFTTESCFSDRGSLKSIMQSNTLTKDEDVQRDLEHSLQMEAYERRIRRLEQEKLELSR
KLQESTQTVQSLHGSSRALSNSNRDKEIKKLNEEIERLKNKIADSNRLERQLEDTVALR
QEREDSTQRLRGLEKQHRVVRQEKEELHKQLVEASERLKSQAKELKDAHQQRKLALQEF
SELNERMAELRAQKQKVSRQLRDKEEEMEVATQKVDAMRQEMRRAEKLRKELEAQLDDA
VAEASKERKLREHSENFCKQMESELEALKVKQGGRGAGATLEHQQEISKIKSELEKKVL
FYEEELVRREASHVLEVKNVKKEVHDSESHQLALQKEILMLKDKLEKSKRERHNEMEEA
VGTIKDKYERERAMLFDENKKLTAENEKLCSFVDKLTAQNRQLEDELQDLAAKKESVAH
WEAQIAEIIQWVSDEKDARGYLQALASKMTEELEALRSSSLGSRTLDPLWKVRRSQKLD
MSARLELQSALEAEIRAKQLVQEELRKVKDANLTLESKLKDSEAKNRELLEEMEILKKK
MEEKFRADTGLKLPDFQDSIFEYFNTAPLAHDLTFRTSSASEQETQAPKPEASPSMSVA
ASEQQEDMARPPQRPSAVPLPTTQALALAGPKPKAHQFSIKSFSSPTQCSHCTSLMVGL
IRQGYACEVCSFACHVSCKDGAPQVCPIPPEQSKRPLGVDVQRGIGTAYKGHVKVPKPT
GVKKGWQRAYAVVCDCKLFLYDLPEGKSTQPGVIASQVLDLRDDEFSVSSVLASDVIHA
TRRDIPCIFRVTASLLGAPSKTSSLLILTENENEKRKWVGILEGLQSILHKNRLRNQ"
misc_feature 1895..1900
/label=SDS
misc_feature 2078..2083
/label=SDS
misc_feature 2370..2375
/label=SDS
misc_feature 2547..2552
/label=SDS
misc_feature 2550..2555
/label=SDS
misc_feature 2749..2754
/label=SDS
misc_feature 2895..2900
/label=SDS
misc_feature 3000..3005
/label=SDS
misc_feature 3426..3431
/label=SDS
misc_feature 3729..3734
/label=SDS
intron 4418..4458
/label=Splicing Donor
misc_feature 4462..4495
/label=lox2272-JT1571
repeat_region 4540..4680
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4540..4669
/label=AAV2 ITR
repeat_region 4541..4681
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 4755..5210
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5492..5596
/gene="bla"
/label=AmpR promoter
CDS 5597..6457
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6628..7216
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatcttc ggccaaggtg cggctcaaga agctggagca
781 gctgctcctg gacgggccct ggcgcaacga gagcgccctg agcgtggaaa cgctgctcga
841 cgtgctcgtc tgcctgtaca ccgagtgcag ccactcggcc ctgcgccgcg acaagtacgt
901 ggccgagttc ctcgagtggg ctaaaccatt tacacagctg gtgaaagaaa tgcagcttca
961 tcgagaagac tttgaaataa ttaaagtaat tggaagaggt gcttttggtg aggttgctgt
1021 tgtcaaaatg aagaatactg aacgaattta tgcaatgaaa atcctcaaca agtgggagat
1081 gctgaaaaga gcagagaccg cgtgcttccg agaggagcgc gatgtgctgg tgaacggcga
1141 ctgccagtgg atcaccgcgc tgcactacgc ctttcaggac gagaaccacc tgtacttagt
1201 catggattac tatgtgggtg gtgatttact gaccctgctc agcaaatttg aagacaagct
1261 tccggaagat atggcgaggt tctacattgg tgaaatggtg ctggccattg actccatcca
1321 tcagcttcat tacgtgcaca gagacattaa acctgacaat gtccttttgg acgtgaatgg
1381 tcatatccgc ctggctgact ttggatcatg tttgaagatg aatgatgatg gcactgtgca
1441 gtcctccgtg gccgtgggca cacctgacta catctcgccg gagatcctgc aggcgatgga
1501 ggacggcatg ggcaaatacg ggcctgagtg tgactggtgg tctctgggtg tctgcatgta
1561 tgagatgctc tatggagaaa cgccgtttta tgcggagtca ctcgtggaga cctatgggaa
1621 gatcatgaac catgaagagc gattccagtt cccatcccat gtcacggatg tatctgaaga
1681 agcgaaggac ctcatccaga gactgatctg cagtagagaa cgccggctgg ggcagaatgg
1741 aatagaggat ttcaaaaagc atgcgttttt tgaaggtcta aattgggaaa atatacgaaa
1801 cctagaagca ccttatattc ctgatgtgag cagtccctct gacacatcca acttcgacgt
1861 ggatgacgac gtgctgagaa acacggaaat attacctcct ggttctcaca caggcttttc
1921 tggattacat ttgccattca ttggttttac attcacaacg gaaagctgtt tttctgatcg
1981 aggctctctg aagagcataa tgcagtccaa cacattaacc aaagatgagg atgtgcagcg
2041 ggacctggag cacagcctgc agatggaagc ttacgagagg aggattcgga ggctggaaca
2101 ggagaagctg gagctgagca ggaagctgca agagtccacc cagaccgtgc agtccctcca
2161 cggctcatct cgggccctca gcaattcaaa ccgagataaa gaaatcaaaa agctaaatga
2221 agaaatcgaa cgcttgaaga ataaaatagc agattcaaac aggctggagc gacagcttga
2281 ggacacagtg gcgcttcgcc aagagcgtga ggactccacg cagcggctgc gggggctgga
2341 gaagcagcac cgcgtggtcc ggcaggagaa ggaggagctg cacaagcaac tggttgaagc
2401 ctcagagcgg ttgaaatccc aggccaagga actcaaagat gcccatcagc agcgaaagct
2461 ggccctgcag gagttctcgg agctgaacga gcgcatggca gagctccgtg cccagaagca
2521 gaaggtgtcc cggcagctgc gagacaagga ggaggagatg gaggtggcca cgcagaaggt
2581 ggacgccatg cggcaggaaa tgcggagagc tgagaagctc aggaaagagc tggaagctca
2641 gcttgatgat gctgttgctg aggcctccaa ggagcgcaag cttcgtgagc acagcgagaa
2701 cttctgcaag caaatggaaa gcgagctgga ggccctcaag gtgaagcaag gaggccgggg
2761 agcgggtgcc accttagagc accagcaaga gatttccaaa atcaaatccg agctggagaa
2821 gaaagtctta ttttatgaag aggaattggt cagacgtgag gcctcccatg tgctagaagt
2881 gaaaaatgtg aagaaggagg tgcatgattc agaaagccac cagctggccc tgcagaaaga
2941 aatcttgatg ttaaaagata agttagaaaa gtcaaagcga gaacggcata acgagatgga
3001 ggaggcagta ggtacaataa aagataaata cgaacgagaa agagcgatgc tgtttgatga
3061 aaacaagaag ctaactgctg aaaatgaaaa gctctgttcc tttgtggata aactcacagc
3121 tcaaaataga cagctggagg atgagctgca ggatctggca gccaagaagg agtcagtggc
3181 ccactgggaa gctcagattg cggaaatcat tcagtgggtc agtgacgaga aagatgcccg
3241 gggttacctt caagctcttg cttccaagat gaccgaagag ctcgaggctt tgaggagttc
3301 tagtctgggg tcaagaacac tggacccgct gtggaaggtg cgccgcagcc agaagctgga
3361 catgtccgcg cggctggagc tgcagtcggc cctggaggcg gagatccggg ccaagcagct
3421 tgtccaggag gagctcagga aggtcaagga cgccaacctc accttggaaa gcaaactaaa
3481 ggattccgaa gccaaaaaca gagaattatt agaagaaatg gaaattttga agaaaaagat
3541 ggaagaaaaa ttcagagcag atactgggct caaacttcca gattttcagg attccatttt
3601 tgagtatttc aacactgctc ctcttgcaca tgacctgaca tttagaacca gctcagctag
3661 tgagcaagaa acacaagctc cgaagccaga agcgtccccg tcgatgtctg tggctgcatc
3721 agagcagcag gaggacatgg ctcggccccc gcagaggcca tccgctgtgc cgttgcccac
3781 cacgcaggcc ctggctctgg ctggaccgaa gccaaaagct caccagttca gcatcaagtc
3841 cttctccagc cctactcagt gcagccactg cacctccctg atggttgggc tgatccggca
3901 gggctacgcc tgcgaggtgt gttcctttgc ttgccacgtg tcctgcaaag acggtgcccc
3961 ccaggtgtgc ccaatacctc ccgagcagtc caagaggcct ctgggcgtgg acgtgcagcg
4021 aggcatcgga acagcctaca aaggccatgt caaggtccca aagcccacgg gggtgaagaa
4081 gggatggcag cgcgcatatg cagtcgtctg tgactgcaag ctcttcctgt atgatctgcc
4141 tgaaggaaaa tccacccagc ctggtgtcat tgcgagccaa gtcttggatc tcagagatga
4201 cgagttttcc gtgagctcag tcctggcctc agatgtcatt catgctacac gccgagatat
4261 tccatgtata ttcagggtga cggcctctct cttaggtgca ccttctaaga ccagctcgct
4321 gctcattctg acagaaaatg agaatgaaaa gaggaagtgg gttgggattc tagaaggact
4381 ccagtccatc cttcataaaa accggctgag gaatcaggta agtatcaagg ttacaagaca
4441 ggtttaagga gaccaataag ctattattcg tataggatac tttatacgaa gttattaagt
4501 tatggagacc ggtgtggcac gtgcggaccg agcggccgca ggaaccccta gtgatggagt
4561 tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc
4621 gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg
4681 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc
4741 aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
4801 gcgcagcgtg accgctacac ttgccagcgc cttagcgccc gctcctttcg ctttcttccc
4861 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
4921 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg
4981 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
5041 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaactcta tctcgggcta
5101 ttcttttgat ttataaggga ttttgccgat ttcggtctat tggttaaaaa atgagctgat
5161 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac
5221 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
5281 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
5341 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
5401 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
5461 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
5521 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
5581 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
5641 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
5701 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
5761 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
5821 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
5881 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
5941 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
6001 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
6061 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
6121 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
6181 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
6241 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
6301 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
6361 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
6421 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
6481 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
6541 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
6601 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
6661 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
6721 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
6781 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
6841 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
6901 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
6961 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
7021 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
7081 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
7141 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
7201 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
7261 gccttttgct cacatgt
//
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