| 质粒名称: | pSY14-ptb |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 90490 |
| 存储实验室: | Sheng Yang |
|
载体骨架基本信息 |
|
| 载体名称: | pSY8 |
| 完整质粒大小: | 4751bp |
| 载体类型: | 细菌表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | sceC |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | unknown (unknown if destroyed) |
| 3’克隆位点: | unknown (unknown if destroyed) |
|
文献引用方法 |
|
| 材料和方法部分: | pSY14-ptb was a gift from Sheng Yang (Addgene plasmid # 90490 ; http://n2t.net/addgene:90490 ; RRID:Addgene_90490) |
| 参考文献部分: | I-SceI-mediated scarless gene modification via allelic exchange in Clostridium. Zhang N, Shao L, Jiang Y, Gu Y, Li Q, Liu J, Jiang W, Yang S. J Microbiol Methods. 2015 Jan;108:49-60. doi: 10.1016/j.mimet.2014.11.004. Epub 2014 Nov 15. 10.1016/j.mimet.2014.11.004 PubMed 25451462 |
pSY14-ptb
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pSY14-ptb载体质粒基本信息
pSY14-ptb质粒图谱载体图谱和pSY14-ptb载体序列质粒序列多克隆位点信息
pSY14-ptb质粒载体简介
pSY14-ptb质粒序列载体序列
LOCUS pSY14-ptb 4751 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION pSY8 derivative with sceC gene under the control of ptb promoter
used for I-SceI-mediated scarless gene modification via allelic
exchange in Clostridium.
ACCESSION .
VERSION .
KEYWORDS pSY14-ptb
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4751)
AUTHORS Zhang N, Shao L, Jiang Y, Gu Y, Li Q, Liu J, Jiang W, Yang S
TITLE I-SceI-mediated scarless gene modification via allelic exchange in
Clostridium.
JOURNAL J Microbiol Methods. 2015 Jan;108:49-60. doi:
10.1016/j.mimet.2014.11.004. Epub 2014 Nov 15.
PUBMED 25451462
REFERENCE 2 (bases 1 to 4751)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..4751
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(356..373)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(527..1115)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(607..626)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(1286..2146)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 1909..1928
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(2147..2251)
/gene="bla"
/label=AmpR promoter
primer_bind 2319..2337
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(2375..2397)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 2497..2516
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 cccttgtcac taagaaaata aatgcagggt aaaatttata tccttcttgt tttatgtttc
61 ggtataaaac actaatatca atttctgtgg ttatactaaa agtcgtttgt tggttcaaat
121 aatgattaaa tatctctttt ctcttccaat tgtctaaatc aattttatta aagttcattt
181 gatatgcctc ctaaattttt atctaaagtg aatttaggag gcttacttgt ctgctttctt
241 cattagaatc aatccttttt taaaagtcaa tattactgta acataaacag ctgcattaat
301 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
361 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
421 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
481 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
541 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
601 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
661 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
721 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
781 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
841 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
901 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
961 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
1021 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
1081 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
1141 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
1201 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
1261 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
1321 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
1381 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
1441 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
1501 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
1561 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
1621 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
1681 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
1741 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
1801 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
1861 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
1921 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
1981 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
2041 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
2101 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
2161 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
2221 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
2281 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
2341 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
2401 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
2461 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
2521 gagtgcacca tatgctcgag ttatttaaga aaagtttcac tactaattgt atttggaagt
2581 ttatacatca tttgtggaat aagatatggt ttaattaaat tgtaaaatat aagatatgac
2641 atactatcaa tataaattat aggtttattt ttatttattt ttacataaca attaagttga
2701 aatttatttc taagtccttt tactaaatat tcaacttctt caaaagtaaa agattgtgta
2761 tttaaaacta tacttttatt agtagaattt ttattataat cccatttacc tccatcatcc
2821 ataaaccaat atgcaagtga cattggagta agataatttt ctactaaatt attaggaatt
2881 gttttcttat tattaacaat aaaaagatta gcaagtttat taaatgcttg atgtttaaaa
2941 gtttgagcac cccatgtaat tacaagattt ccaagatgat taactctttg ttttttatgt
3001 ggaggtgaaa gtacccattg atcataaaga agacaaacat gatccatata tgctttattt
3061 ttccattcaa attgcataca atatgtttta ccttcatctc tacttcttat ataagcatct
3121 ccaagaataa gaccaattcc tgcttcaaat tgttctatat taagttctat aagttgtgat
3181 ttatattctt taagaagttt actattagga ccaagattca ttacttgatt cttttgatgc
3241 atatgtcgac actccctttt actatttaat tatctatgtt aaatgattaa cgtgctcatt
3301 tatattttaa caaacttttc acatgaagtt aagtttttta gaaaattatt tatattttat
3361 agaattcccg gggatccgtc gacctgcagc caagcttaat cgcatttcat agattgacct
3421 cccaataact acgtggtgtt attgggaggt caatctattt catttgcctc ttgctcaaag
3481 ttcccaaatt cgagtaagag gtatttttgt ttttggtcgt cgcctctcat tagtagttca
3541 gggtttaaca ttaatactcc agtttttctt tttataatat ttccttcttc taagatttta
3601 agtgttgtta ttactgtttg tagacttgtt cctgtagctt ttgctatttc tcttgttgta
3661 gctatcattg tattgttact taagtggaca ttatctagga tatagttaac gattttaagt
3721 ttttttccgc caatcatatc taacatactt attaattgca ctatatatgc ctttacgaag
3781 ttaccagacg tttgtttacg gtataacttg tctacctcta tgacttctcc actttcttcg
3841 tctatgagcc tctgagagcc tttatagact gttccatatc tttctttcat ctttttctca
3901 ctccttattt taaactattc taactatatc ataactgttc taaaaaaaaa agaacatttg
3961 ttaaaagaaa ttagaacaaa atgagtgaaa aattagaaca aacaaattcc ttataaacct
4021 tatcatctca acctatatta agattttacc tagttgaatc ttcttttcta tataaagcgt
4081 cggagcatat cagggggtta tctaacgtaa atgctaccct tcggctcgct ttcgctcggc
4141 attgacgtca gatactgcac cccctgaacc cccatgctcc aacagcaaaa aggaaacttt
4201 ttgctgcttt tccgacgctt attcgcttcg ctcatattta tatagaaaag aagtgaatgc
4261 gcaaaagaca taatcgattt ataaaagcca gtcattaggc ctatctgaca attcctgaat
4321 agagttcata aacaatcctg catgataacc atcacaaaca gaatgatgta cctgtaaaga
4381 tagcggtaaa tatattgaat tacctttatt aatgaatttt cctgctgtaa taatgggtag
4441 aaggtaatta ctattattat tgatatttaa gttaaaccca gtaaatgaag tccatggaat
4501 aatagaaaga gaaaaagcat tttcaggtat aggtgttttg ggaaacaatt tccccgaacc
4561 attatatttc tctacatcag aaaggtataa atcataaaac tctttgaagt cattctttac
4621 aggagtccaa ataccagaga atgttttaga tacaccatca aaaattgtat aaagtggctc
4681 taacttatcc caataaccta actctccgtc gctattgtaa ccagttctaa aagctgtatt
4741 tgagtttatc a
//
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