PB-iETV2-HA

LOCUS       PB-iETV2-HA            8596 bp DNA     circular SYN 16-MAR-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8596)
  AUTHORS   Amane
  TITLE     Direct Submission
  JOURNAL   Exported Aug 15, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8596
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          2824..2910
                     /note="/dnas_title=3xHA-TurboID-NLS in pUC-GW-Amp"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     850..869
                     /label=18-3ITR-F
     misc_feature    870..1103
                     /label=3' ITR
                     /note="piggyBac 3' inverted terminal repeatApplication 
                     Notes:Recognized by PBase transposase; DNA flanked by 
                     piggyBac 5' ITR and 3' ITR can be transposed by PBase into 
                     TTAA sites.
                     "
     primer_bind     complement(1086..1120)
                     /label=19-3UTR-R
     primer_bind     1277..1296
                     /label=PAN469
     promoter        1385..1763
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    1393..1411
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1429..1447
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1465..1483
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1501..1519
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1537..1555
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1573..1591
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1609..1627
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1676..1699
                     /label=PAN045
     misc_feature    1764..1788
                     /label=MCS
     primer_bind     1769..1818
                     /label=73-ETV2-F
     regulatory      1789..1798
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1795..2823
                     /codon_start=1
                     /label=ETV2 ORF 1029bp
                     /translation="MDLWNWDEASPQEVPPGNKLAGLEGAKLGFCFPDLALQGDTPTAT
                     AETCWKGTSSSLASFPQLDWGSALLHPEVPWGAEPDSQALPWSGDWTDMACTAWDSWSG
                     ASQTLGPAPLGPGPIPAAGSEGAAGQNCVPVAGEATSWSRAQAAGSNTSWDCSVGPDGD
                     TYWGSGLGGEPRTDCTISWGGPAGPDCTTSWNPGLHAGGTTSLKRYQSSALTVCSEPSP
                     QSDRASLARCPKTNHRGPIQLWQFLLELLHDGARSSCIRWTGNSREFQLCDPKEVARLW
                     GERKRKPGMNYEKLSRGLRYYYRRDIVRKSGGRKYTYRFGGRVPSLAYPDCAGGGRGAE
                     TQY"
     misc_feature    complement(2801..2820)
                     /label=3,007 R
                     /note="Geneious type: primer_bind_reverse
                     %GC=60.0
                     Hairpin_Tm=None
                     Pair_Dimer_Tm=4.2
                     Product_Size=993
                     Self_Dimer_Tm=None
                     Sequence=TTGTGTCTCTGCTCCCCGTC
                     Tm=61.8
                     annotation_group=1464808224377366000f7b22693-71df-470e-9 
                     a6c-ab1e2e0f88d6PB-AarI - pDONR221-wehave - XM_005258652 - 
                     ETV2 with attB sites Entry clone Expression clone
                     created_by=primer3"
     primer_bind     complement(2802..2843)
                     /label=73-ETV2-R
     CDS             2824..2850
                     /codon_start=1
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     2824..2844
                     /label=73-HA-F
     CDS             2854..2880
                     /codon_start=1
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2884..2910
                     /codon_start=1
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(2893..2933)
                     /label=73-HA-R
     polyA_signal    2926..3133
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(3130..3145)
                     /label=PAN474
     primer_bind     complement(3203..3219)
                     /label=PAN481
     promoter        3210..3421
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     primer_bind     3291..3310
                     /label=PAN470
     CDS             3736..4479
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     3802..3821
                     /label=PAN471
     primer_bind     4319..4338
                     /label=PAN472
     CDS             4480..4533
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4534..5133
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     4679..4698
                     /label=F-Puro-qPCR
     primer_bind     complement(4791..4807)
                     /label=R-Puro-qPCR
     polyA_signal    5166..5287
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5592..5611)
                     /label=PAN469
     misc_feature    complement(5776..6088)
                     /label=5' ITR
                     /note="piggyBac 5' inverted terminal repeatRecognized by 
                     PBase transposase; DNA flanked by piggyBac 5' ITR and 3' 
                     ITR can be transposed by PBase into TTAA sites.
                     "
     primer_bind     complement(6575..6591)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6599..6615
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6623..6653)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6668..6689
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(6977..7565)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7736..8596)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggccacta gtctttcgtc ttcaagaatt
     1381 cctcgagttt actccctatc agtgatagag aacgtatgaa gagtttactc cctatcagtg
     1441 atagagaacg tatgcagact ttactcccta tcagtgatag agaacgtata aggagtttac
     1501 tccctatcag ggatagagaa cgtatgacca gtttactccc tatcagtgat agagaacgta
     1561 tctacagttt actccctatc agtgatagag aacgtatatc cagtttactc cctatcagtg
     1621 atagagaacg tataagcttt aggcgtgtac ggtgggcgcc tataaaagca gagctcgttt
     1681 agtgaaccgt cagatcgcct ggagcaattc cacaacactt ttgtcttata ccaactttcc
     1741 gtaccacttc ctaccctcgt aaaggtctag agctagcggc gcgccgttgc caccatggat
     1801 ttgtggaatt gggatgaggc atcacctcaa gaggttccgc ctggtaacaa acttgcaggg
     1861 ctcgaaggag ccaaattagg cttctgtttc cctgatctgg cactccaagg ggacacgccg
     1921 acagcgacag cagagacatg ctggaaaggt acaagctcat ccctggcaag cttcccacag
     1981 ctggactggg gctccgcgtt actgcaccca gaagttccat ggggggcgga gcccgactct
     2041 caggctcttc cgtggtccgg ggactggaca gacatggcgt gcacagcctg ggactcttgg
     2101 agcggcgcct cgcagaccct gggccccgcc cctctcggcc cgggccccat ccccgccgcc
     2161 ggctccgaag gcgccgcggg ccagaactgc gtccccgtgg cgggagaggc cacctcgtgg
     2221 tcgcgcgccc aggccgccgg gagcaacacc agctgggact gttctgtggg gcccgacggc
     2281 gatacctact ggggcagtgg cctgggcggg gagccgcgca cggactgtac catttcgtgg
     2341 ggcgggcccg cgggcccgga ctgtaccacc tcctggaacc cggggctgca tgcgggtggc
     2401 accacctctt tgaagcggta ccagagctca gctctcaccg tttgctccga accgagcccg
     2461 cagtcggacc gtgccagttt ggctcgatgc cccaaaacta accaccgagg tcccattcag
     2521 ctgtggcagt tcctcctgga gctgctccac gacggggcgc gtagcagctg catccgttgg
     2581 actggcaaca gccgcgagtt ccagctgtgc gaccccaaag aggtggctcg gctgtggggc
     2641 gagcgcaaga gaaagccggg catgaattac gagaagctga gccggggcct tcgctactac
     2701 tatcgccgcg acatcgtgcg caagagcggg gggcgaaagt acacgtaccg cttcgggggc
     2761 cgcgtgccca gcctagccta tccggactgt gcgggaggcg gacggggagc agagacacaa
     2821 tactacccgt atgatgttcc ggattacgct ggctatccct acgacgtgcc cgactatgcc
     2881 gggtacccct atgacgtccc agactacgca tgaggtaccc ctcgactgtg ccttctagtt
     2941 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     3001 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     3061 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     3121 ggcatgctgg ggatgcggtg ggctcttcga atttaaatcg gatccagctg aatctaagtg
     3181 gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga
     3241 gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc gcggggtaaa
     3301 ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta
     3361 tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca
     3421 gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg
     3481 ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc
     3541 gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc
     3601 ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact
     3661 ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tctaagctgt gaccggcgcc
     3721 tactctagag ccgccatgtc tagactggac aagagcaaag tcataaactc tgctctggaa
     3781 ttactcaatg gagtcggtat cgaaggcctg acgacaagga aactcgctca aaagctggga
     3841 gttgagcagc ctaccctgta ctggcacgtg aagaacaagc gggccctgct cgatgccctg
     3901 ccaatcgaga tgctggacag gcatcatacc cactcctgcc ccctggaagg cgagtcatgg
     3961 caagactttc tgcggaacaa cgccaagtca taccgctgtg ctctcctctc acatcgcgac
     4021 ggggctaaag tgcatctcgg cacccgccca acagagaaac agtacgaaac cctggaaaat
     4081 cagctcgcgt tcctgtgtca gcaaggcttc tccctggaga acgcactgta cgctctgtcc
     4141 gccgtgggcc actttacact gggctgcgta ttggaggaac aggagcatca agtagcaaaa
     4201 gaggaaagag agacacctac caccgattct atgcccccac ttctgaaaca agcaattgag
     4261 ctgttcgacc ggcagggagc cgaacctgcc ttccttttcg gcctggaact aatcatatgt
     4321 ggcctggaga aacagctaaa gtgcgaaagc ggcgggccga ccgacgccct tgacgatttt
     4381 gacttagaca tgctcccagc cgatgccctt gacgactttg accttgatat gctgcctgct
     4441 gacgctcttg acgattttga ccttgacatg ctccccgggg agggcagagg aagtcttcta
     4501 acatgcggtg acgtggagga gaatcccggc cctatgaccg agtacaagcc cacggtgcgc
     4561 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac
     4621 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg
     4681 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
     4741 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
     4801 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
     4861 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
     4921 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
     4981 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
     5041 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
     5101 acctggtgca tgacccgcaa gcccggtgcc tgaaatcaac ctctggatta caaaatttgt
     5161 gggtaaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     5221 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     5281 tatcttatca tgtctggaat tgactcaaat gatgtcaatt agtctatcag aagctcatct
     5341 ggtctccctt ccgggggaca agacatccct gtttaatatt taaacagcag tgttcccaaa
     5401 ctgggttctt atatcccttg ctctggtcaa ccaggttgca gggtttcctg tcctcacagg
     5461 aacgaagtcc ctaaagaaac agtggcagcc aggtttagcc ccggaattga ctggattcct
     5521 tttttagggc ccattggtat ggctttttcc ccgtatcccc ccaggtgtct gcaggctcaa
     5581 agagcagcga gaagcgttca gaggaaagcg atcccgtgcc accttccccg tgcccgggct
     5641 gtccccgcac gctgccggct cggggatgcg gggggagcgc cggaccggag cggagccccg
     5701 ggcggctcgc tgctgccccc tagcggggga gggacgtaat tacatccctg ggggctttgg
     5761 gggggggctg tccctgatat ctataacaag aaaatatata tataataagt tatcacgtaa
     5821 gtagaacatg aaataacaat ataattatcg tatgagttaa atcttaaaag tcacgtaaaa
     5881 gataatcatg cgtcattttg actcacgcgg tcgttatagt tcaaaatcag tgacacttac
     5941 cgcattgaca agcacgcctc acgggagctc caagcggcga ctgagatgtc ctaaatgcac
     6001 agcgacggat tcgcgctatt tagaaagaga gagcaatatt tcaagaatgc atgcgtcaat
     6061 tttacgcaga ctatctttct agggttaatc tagctgcatc aggatcatat cgtcgggtct
     6121 tttttccggc tcagtcatcg cccaagctgg cgctatctgg gcatcgggga ggaagaagcc
     6181 cgtgcctttt cccgcgaggt tgaagcggca tggaaagagt ttgccgagga tgactgctgc
     6241 tgcattgacg ttgagcgaaa acgcacgttt accatgatga ttcgggaagg tgtggccatg
     6301 cacgccttta acggtgaact gttcgttcag gccacctggg ataccagttc gtcgcggctt
     6361 ttccggacac agttccggat ggtcagcccg aagcgcatca gcaacccgaa caataccggc
     6421 gacagccgga actgccgtgc cggtgtgcag attaatgaca gcggtgcggc gctgggatat
     6481 tacgtcagcg aggacgggta tcctggctgg atgccgcaga aatggacatg gataccccgt
     6541 gagttacccg gcgggcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     6601 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     6661 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     6721 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     6781 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     6841 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     6901 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     6961 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     7021 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     7081 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     7141 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     7201 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     7261 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     7321 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     7381 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     7441 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     7501 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     7561 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     7621 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     7681 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     7741 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     7801 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     7861 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     7921 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     7981 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     8041 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     8101 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     8161 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     8221 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     8281 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     8341 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     8401 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     8461 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     8521 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     8581 aatgttgaat actcat
//