pAAV-Syn-FLAGx3-SYT1wt

LOCUS      pAAV-Syn-FLAGx3-SYT1wt              5791 bp DNA     circular SYN 03-MAR-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5791)
  AUTHORS   SnapGene License
  TITLE     Direct Submission
  JOURNAL   Exported Aug 27, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..5791
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    311..800
                     /label=Syn
     promoter        349..796
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     primer_bind     734..755
                     /label=Syn-F
     misc_feature    812..817
                     /label=Kozak sequence
     CDS             818..886
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="MDYKDHDGDYKDHDIDYKDDDDK"
     CDS             887..895
                     /label=Linker
     CDS             896..2164
                     /codon_start=1
                     /label=SYT1/I368T
                     /translation="MVSESHHEALAAPPVTTVATVLPSNATEPASPGEGKEDAFSKLKE
                     KFMNELHKIPLPPWALIAIAIVAVLLVLTCCFCICKKCLFKKKNKKKGKEKGGKNAINM
                     KDVKDLGKTMKDQALKDDDAETGLTDGEEKEEPKEEEKLGKLQYSLDYDFQNNQLLVGI
                     IQAAELPALDMGGTSDPYVKVFLLPDKKKKFETKVHRKTLNPVFNEQFTFKVPYSELGG
                     KTLVMAVYDFDRFSKHDIIGEFKVPMNTVDFGHVTEEWRDLQSAEKEEQEKLGDICFSL
                     RYVPTAGKLTVVILEAKNLKKMDVGGLSDPYVKIHLMQNGKRLKKKKTTIKKNTLNPYY
                     NESFSFEVPFEQIQKVQVVVTVLDYDKIGKNDAIGKVFVGYNSTGAELRHWSDMLANPR
                     RPIAQWHTLQVEEEVDAMLAVKK"
     misc_feature    911..1234
                     /label=N-terminal domain
     misc_feature    1322..1690
                     /label=C2A domain
     misc_feature    1715..2122
                     /label=C2B domain
     misc_feature    1997..1999
                     /label=Ile368
     misc_feature    2189..2777
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(2660..2671)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2795..3002
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   3054..3194
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      3269..3724
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4006..4110
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4111..4971
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5142..5730
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgttgcaaa gatggataaa gttttaaaca gagaggaatc
      181 tttgcagcta atggaccttc taggtcttga aaggagtggg aattggctcc ggtgcccgtc
      241 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcagca
      301 aattaattaa tggttaatta atctagactg cagagggccc tgcgtatgag tgcaagtggg
      361 ttttaggacc aggatgaggc ggggtggggg tgcctacctg acgaccgacc ccgacccact
      421 ggacaagcac ccaaccccca ttccccaaat tgcgcatccc ctatcagaga gggggagggg
      481 aaacaggatg cggcgaggcg cgtgcgcact gccagcttca gcaccgcgga cagtgccttc
      541 gcccccgcct ggcggcgcgc gccaccgccg cctcagcact gaaggcgcgc tgacgtcact
      601 cgccggtccc ccgcaaactc cccttcccgg ccaccttggt cgcgtccgcg ccgccgccgg
      661 cccagccgga ccgcaccacg cgaggcgcga gatagggggg cacgggcgcg accatctgcg
      721 ctgcggcgcc ggcgactcag cgctgcctca gtctgcggtg ggcagcggag gagtcgtgtc
      781 gtgcctgaga gcgcagtcga gaggatccta agccaccatg gactacaaag accatgacgg
      841 tgattataaa gatcatgaca tcgactacaa ggatgacgat gacaagggag gaagcatggt
      901 gagcgagagt caccatgagg ccctggcagc cccgcctgtc accactgtcg cgactgttct
      961 gccaagcaat gccacagagc cagccagtcc tggagaagga aaggaagatg cattttctaa
     1021 gctgaaggag aagtttatga atgagttgca taaaattcca ttgccaccgt gggccttaat
     1081 tgcaatagcc atagtcgcag tccttttagt cctgacctgc tgcttttgta tctgtaagaa
     1141 atgtttgttc aaaaagaaaa acaagaagaa gggaaaggaa aaaggaggga agaatgccat
     1201 taacatgaaa gatgtaaaag acttagggaa gacgatgaaa gatcaggccc tcaaggatga
     1261 tgatgctgaa actggattga cagatggaga agaaaaagaa gaacccaaag aagaggagaa
     1321 actgggaaaa cttcagtatt cactggatta tgatttccaa aataaccagc tgctggtagg
     1381 gatcattcag gctgccgaac tgcccgcctt ggacatgggg ggcacatctg atccttacgt
     1441 gaaagtgttt ctgctacctg ataagaagaa gaaatttgag acaaaagtcc accgaaaaac
     1501 ccttaatcct gtcttcaatg agcaatttac tttcaaggta ccatactcgg aattgggtgg
     1561 caaaacccta gtgatggctg tatatgattt tgatcgtttc tctaagcatg acatcattgg
     1621 agaatttaaa gtccctatga acacagtgga ttttggccat gtaactgagg aatggcgtga
     1681 cctgcaaagt gctgagaagg aagagcaaga gaaattgggt gatatctgct tctcccttcg
     1741 ctacgtacct actgctggta agctgactgt tgtcattctg gaggcaaaga acctgaagaa
     1801 gatggatgtg ggtggcttat ccgatcctta tgtgaagatt catctgatgc agaatggtaa
     1861 gaggctgaag aagaaaaaga caacaattaa aaagaacaca cttaacccct actacaatga
     1921 gtcattcagc tttgaagtac cttttgaaca aatccagaaa gtgcaggtgg tggtaactgt
     1981 tttggactat gacaagattg gcaagaacga tgccatcggc aaagtctttg tgggctacaa
     2041 cagcaccggc gcggagctgc gacactggtc agacatgctg gccaacccca ggcgacctat
     2101 tgcccagtgg cacaccctgc aggtagagga ggaagttgat gccatgctgg ccgtcaagaa
     2161 gtaggaattc cgtacaaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag
     2221 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     2281 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     2341 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     2401 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     2461 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     2521 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     2581 gaaatcatcg tcctttcctt ggctgctcgc ctatgttgcc acctggattc tgcgcgggac
     2641 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     2701 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     2761 ttgggccgcc tccccgcatc gataccggct cgagctgtgc cttctagttg ccagccatct
     2821 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     2881 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     2941 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     3001 gatgcggtct cgagctgatt ttgtaggtaa ccacgtgcgg accgagcggc cgcaggaacc
     3061 cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg
     3121 accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg
     3181 cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     3241 acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg
     3301 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccttagc gcccgctcct
     3361 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     3421 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     3481 gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     3541 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     3601 tctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggt ctattggtta
     3661 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca
     3721 attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     3781 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     3841 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     3901 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     3961 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     4021 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     4081 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     4141 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     4201 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     4261 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     4321 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     4381 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     4441 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     4501 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     4561 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     4621 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     4681 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     4741 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     4801 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     4861 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     4921 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     4981 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     5041 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     5101 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     5161 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     5221 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     5281 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     5341 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     5401 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     5461 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     5521 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     5581 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     5641 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     5701 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     5761 ctggcctttt gctggccttt tgctcacatg t
//