| 质粒名称: | pSF11-wmNeonGreen |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000100 |
| 产品用途: | Expresses mNeonGreen in C. elegans neurons |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pSF11 |
| 表达系统: | 线虫表达 |
| 启动子: | tag168 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a，TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | mNeonGreen |
| 物种来源: |
Synthetic
|
| 密码子优化: | 蠕虫表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Quantitative assessment of near-infrared fluorescent proteins
Zhang H., Papadaki S., Sun X., Wang X., Drobizhev M., Yao L., Rehbock M., Köster R.W., Wu L., Namikawa K., Piatkevich K.D.
Nat. Methods 2023 2010 20, 1605–1616 (2023)
|
买家导航
pSF11-wmNeonGreen载体质粒基本信息
pSF11-wmNeonGreen质粒图谱载体图谱和pSF11-wmNeonGreen载体序列质粒序列多克隆位点信息
pSF11-wmNeonGreen质粒载体简介
pSF11-wmNeonGreen质粒序列载体序列
LOCUS pSF11-wmNeonGreen 6907 bp DNA circular SYN 21-APR-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6907)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 23, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6907
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 54..2717
/label=tag-168 promoter
primer_bind 2796..2816
/label=pSF11-Fw-Seq
misc_feature 2834..2837
/label=codon adapted insert for smGFP-myc
/note="codon adapted by
https://worm.mpi-cbg.de/codons/cgi-bin/optimize.py
Intron * 2 (3 requested, but 1 is inside myc regions and
thus removed)
CAI set to 0.60
Check all 3 boxes
"
CDS 2838..3650
/codon_start=1
/product="bright monomeric yellow-green fluorescent protein
derived from LanYFP (Shaner et al., 2013)"
/label=mNeonGreen
/translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
GASLTVNYRYTYEGSHIKVSLNIYILTNPDYLNFQGEAQVKVSLNSSVLTNHTYLNFQG
TGFPADGPVMTNSLTAADWCRSKKTYPNDKTIISTFKWSYTTGNGKRYRSTARTTYTFA
KPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQKAFTDVMGMDELYK"
primer_bind complement(3692..3708)
/label=pSF11-Rv-Seq
promoter 4786..4890
/gene="bla"
/label=AmpR promoter
CDS 4891..5751
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5846..6528
/label=ColE1 origin
primer_bind complement(6607..6625)
/label=0507-619-R
protein_bind 6798..6819
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 6834..6864
/label=lac promoter
/note="promoter for the E. coli lac operon"
misc_binding 6868..6890
/label=LacO
protein_bind 6872..6888
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind join(6896..6907,1..5)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 atgaccatga ttacgccaag cttgcatgcg cggccgcact gactgggccg gccggatcca
61 taggtatgcc tgtcattctc tatttttaaa cgttcctgca gcagtagcac aacagagcca
121 gcagttttcc tcttcttctc catcttcttc tgcttcttcc tctcttccca aaaaaaaaaa
181 aaaaaaaaaa gtgagggggc tcactgcccc cccccccctc ccccccccct cttctccttg
241 aagctcatcc agacgtcgct gtcctcctcc tcctcgtccc cggggcgctg tgtgtgtgtg
301 tgtgtgctcc gtgagcatgt agcagcttca gcccatttaa aggcgcacac tctctgcatc
361 tctgcgtgtc tcgacactcg ttttttctct tcttttctgc tgcacacaca cacacacaca
421 cacacacgct catgccagag cacttcttgc tccggggggc tccgcgcgga gcccgagagg
481 acctcctcct cctttttctt cttcgctttt tagttcttct tgcctcggtc tcgccccttg
541 ttttgctctc catgtagtat atacatctgg gtgtgtgtgt gtgtgtgcac ctttggagca
601 ttttttgaac ttttccagaa ctttggagca acattttcag cttgttcagt gcacttttta
661 ggctgaaaat cctattttta ggttttcgaa agtttttttg gagcattttt cgtgcaattt
721 aggtgcaaaa ttaaaaaaaa gggttttgta gtgaattaaa atttttttga aactttcgcc
781 tttttaaaaa aatgtttcta atatctttaa aaattggaat attttggaaa acaatttgtt
841 ttcaattggc aatttattaa ttctgagctt ctgagcgtaa attttttttt attttggagc
901 aattctgaga tgtcttagtg catttttcgg gcatacataa aaaaaaattt atcttaataa
961 taaaaacctt aaatttaggc ttcaccgtct ggaaattcaa ttttttcagc tgaaaaactt
1021 tcaaaaaaaa aaaaattaaa ttttcacatt tattttctct ggaattttgg agcaatttca
1081 aagctgtctt agtgcatttt tagtacacaa atattttttc atttttctta aattttaata
1141 atctaagctt aaataaaggc ttcaccgtct gaaaatgcaa cttttttcag atgaaatatt
1201 aaaaaaaaaa acctctttta ttaattttgt gctccaaact ttcccacggt acgcaaatca
1261 tgtaatatgt acttttaaac attatttttc caatcaaaaa tatccaacag cttttcctta
1321 caatttgaaa atttttatta aaatttcaaa aaaatttttt ttttttttta atttcgaatt
1381 tttttaaact tgttgtaact tttttccctg tcggcgccaa ttaacaaaat acgtagaatt
1441 ttgcgcttat tgttttgtta gttaacaaat ttttgacaac tttcttatca gctgagattt
1501 aggtgtttca gttcgcaatc tgcgtctctc cttgctctcc tctattttaa aacccccata
1561 actttgttct tgtgagagtt atcaaaaaat tgtcaactaa caaaatatag agcacaaaaa
1621 tatgcacatt ttgtatgttg acaatttttt gatacctttt ttagttttcg agatatctta
1681 gttttagttt agctgtacct ttttctggca aaaaacttcc aaatttcggc aatttttcgg
1741 caaatttccc ggcaaatttc gccaagtaca tcgggctctc agacaagtgg tctgtgagtg
1801 gaagtcgata aaagtagttt acgtgctctc cgtccccccg cttcttctcc gtctctgctt
1861 ctatttctcc attgactagt cccactactt tttacggggc ggagggctct cttgcactcc
1921 agcttcttct ctttttgtgc tcttttttgc ccgtatgtgg gtgctcattt atttttcttt
1981 ggttttaaaa taaattcggt ttattaggct actcgtaaaa atgaagaaaa aacttcaaat
2041 tcaaacgaca ttaaaaaaaa atttaataaa aattttttga attgctcaaa agccccacct
2101 atatatatat atagctccgc ctacttctcg cacagacccc gcccactttt tgactccgcc
2161 cccttattgg caaaagttct tgaaaaaaat aaaaataaaa attcaaattg aaacaatttt
2221 tttttaatga agtagttttt ttctaaattg atgaaaattt ctaaacagtt tacaagcacc
2281 gcccactttt tgactccgcc cctttgttgg aaaaattctt gtaaaaaaaa ataaaaaata
2341 aaagcttctc tttcaaacga tttttagttg gtttttgtat gaaaagtttt tttcctaaaa
2401 tagaaatgtc tgaataattt gcaagctccg cccttttctt acccagactc cgccccttta
2461 ttgactaatt tttttttgtc catttacctg aagttcaaaa aaaaaattta cagtttcata
2521 gagtaataat ttttcaaaaa tctctttttc caatgtttaa tgtgtcatta taaacccgcc
2581 cagttttaag ctccgcccat ttaggccccg cccatttatg acccaaatat ttttttctct
2641 attcacccgt ggatttccct ccaacaagtt accccaaaaa taaaaataaa aattcctgca
2701 ggcgcccacc gggatccggc gcgcctctag aggatccccg ggattggcca aaggacccaa
2761 aggtatgttt cgaatgatac taacataaca tagaacattt tcaggaggac ccttggctag
2821 cgtcgacggt accaaaaatg gtttcaaaag gagaggaaga taacatggct agtcttccag
2881 ctacccatga gcttcacata ttcggaagca tcaatggagt tgacttcgac atggttggac
2941 agggaacggg aaatccaaat gacggatacg aggagctcaa tcttaaatcc acgaaaggag
3001 atctccaatt ctccccatgg atactcgttc cacacattgg atacggattc caccagtacc
3061 tcccataccc agacggaatg agcccattcc aagctgccat ggtcgacgga agcggatacc
3121 aagtccaccg cacgatgcaa ttcgaggatg gagctagcct caccgttaac tatcgctaca
3181 cgtacgaagg aagccacatc aaggtaagtt taaacatata tatactaact aaccctgatt
3241 atttaaattt tcagggagag gcccaagtta aggtaagttt aaacagttcg gtactaacta
3301 accatacata tttaaatttt cagggaactg gattcccagc cgacggacca gtcatgacga
3361 acagccttac ggctgctgac tggtgccgct ctaaaaaaac ttatccaaac gataaaacta
3421 tcatctctac gttcaagtgg agctacacga cgggaaacgg aaaacgctac cgctccacgg
3481 cccgcacgac ttatactttc gctaagccaa tggccgctaa ttatcttaaa aaccagccaa
3541 tgtacgtctt ccgcaaaacc gaactcaagc actctaaaac ggaacttaac ttcaaagagt
3601 ggcagaaggc cttcaccgac gtcatgggaa tggatgaact ttataaataa gagctccgca
3661 tcggccgctg tcatcagatc gccatctcgc gcccgtgcct ctgacttcta agtccaatta
3721 ctcttcaaca tccctacatg ctctttctcc ctgtgctccc accccctatt tttgttatta
3781 tcaaaaaaac ttcttcttaa tttctttgtt ttttagcttc ttttaagtca cctctaacaa
3841 tgaaattgtg tagattcaaa aatagaatta attcgtaata aaaagtcgaa aaaaattgtg
3901 ctccctcccc ccattaataa taattctatc ccaaaatcta cacaatgttc tgtgtacact
3961 tcttatgttt tttttacttc tgataaattt tttttgaaac atcatagaaa aaaccgcaca
4021 caaaatacct tatcatatgt tacgtttcag tttatgaccg caatttttat ttcttcgcac
4081 gtctgggcct ctcatgacgt caaatcatgc tcatcgtgaa aaagttttgg agtatttttg
4141 gaatttttca atcaagtgaa agtttatgaa attaattttc ctgcttttgc tttttggggg
4201 tttcccctat tgtttgtcaa gagtttcgag gacggcgttt ttcttgctaa aatcacaagt
4261 attgatgagc acgatgcaag aaagatcgga agaaggtttg ggtttgaggc tcagtggaag
4321 gtgagtagaa gttgataatt tgaaagtgga gtagtgtcta tggggttttt gccttaaatg
4381 acagaataca ttcccaatat accaaacata actgtttcct actagtcggc cgtacgggcc
4441 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
4501 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
4561 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
4621 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
4681 caggcggcct taattaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
4741 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
4801 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
4861 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
4921 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
4981 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
5041 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
5101 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
5161 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
5221 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
5281 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
5341 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
5401 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
5461 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
5521 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
5581 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
5641 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
5701 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
5761 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
5821 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
5881 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
5941 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
6001 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
6061 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
6121 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
6181 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
6241 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
6301 ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
6361 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
6421 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
6481 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
6541 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
6601 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
6661 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
6721 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
6781 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
6841 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
6901 aacagct
//
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