pAAVLINKv2-SCP1-Cre-UDeg3a-3'-UNC13A-FLAG

LOCUS       pAAVLINKv2-SCP1-Cre-UDeg3a-3'-UNC13A-FLAG                6960 bp DNA     circular SYN 17-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6960)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported May 15, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6960
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     misc_feature    145..225
                     /label=SCP1
     CDS             237..1265
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1281..1370
                     /codon_start=1
                     /label=UDeg3a
                     /translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
     misc_feature    1295..1300
                     /label=SDS
     misc_feature    1298..1303
                     /label=SDS
     intron          1374..1414
                     /label=Splicing Donor
     protein_bind    1421..1454
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1455..1546
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1547..3673
                     /codon_start=1
                     /label=UNC13A
                     /translation="VVKDCVKACLNSTYEYIFNNCHELYSREYQTDPAKKGEVLPEEQG
                     PSIKNLDFWSKLITLIVSIIEEDKNSYTPCLNQFPQELNVGKISAEVMWNLFAQDMKYA
                     MEEHDKHRLCKSADYMNLHFKVKWLYNEYVTELPAFKDRVPEYPAWFEPFVIQWLDENE
                     EVSRDFLHGALERDKKDGFQQTSEHALFSCSVVDVFSQLNQSFEIIKKLECPDPQIVGH
                     YMRRFAKTISNVLLQYADIISKDFASYCSKEKEKVPCILMNNTQQLRVQLEKMFEAMGG
                     KELDAEASDILKELQVKLNNVLDELSRVFATSFQPHIEECVKQMGDILSQVKGTGNVPA
                     SACSSVAQDADNVLQPIMDLLDSNLTLFAKICEKTVLKRVLKELWKLVMNTMEKTIVLP
                     PLTDQTMIGNLLRKHGKGLEKGRVKLPSHSDGTQMIFNAAKELGQLSKLKDHMVREEAK
                     SLTPKQCAVVELALDTIKQYFHAGGVGLKKTFLEKSPDLQSLRYALSLYTQATDLLIKT
                     FVQTQSAQGLGVEDPVGEVSVHVELFTHPGTGEHKVTVKVVAANDLKWQTSGIFRPFIE
                     VNIIGPQLSDKKRKFATKSKNNSWAPKYNESFQFTLSADAGPECYELQVCVKDYCFARE
                     DRTVGLAVLQLRELAQRGSAACWLPLGRRIHMDDTGLTVLRILSQRSNDEVAKEFVKLK
                     SDTRSAEEGGAAPAP"
     misc_feature    2034..2039
                     /label=SDS
     misc_feature    2286..2291
                     /label=SDS
     misc_feature    3648..3653
                     /label=SDS
     CDS             3680..3745
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3749..4180
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    3893..3898
                     /label=SDS
     repeat_region   4223..4363
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4223..4352
                     /label=AAV2 ITR
     rep_origin      4438..4893
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5175..5279
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5280..6140
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6311..6899
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
      181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
      241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
      301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
      361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
      421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
      481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
      541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
      601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
      661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
      721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
      781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
      841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
      901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
      961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
     1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
     1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
     1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
     1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
     1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
     1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
     1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
     1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtgg taaaggactg
     1561 tgtgaaagcc tgccttaatt ctacctacga gtacatcttc aataactgcc atgaactgta
     1621 cagccgggag taccagacag acccggccaa gaagggggaa gttctcccag aggaacaggg
     1681 gcccagcatc aagaacctcg acttctggtc caagctgatt accctcatag tgtccatcat
     1741 tgaggaagac aagaattcct acactccctg cctcaaccag tttccccagg agctgaatgt
     1801 gggtaaaatc agcgctgaag tgatgtggaa tctgtttgcc caagacatga agtacgccat
     1861 ggaggagcac gacaagcatc gtctatgcaa gagtgccgac tacatgaacc tccacttcaa
     1921 ggtgaaatgg ctctacaatg agtatgtgac ggaacttccc gccttcaagg accgcgtgcc
     1981 tgagtaccct gcatggtttg aacccttcgt catccagtgg ctggatgaga atgaggaggt
     2041 gtcccgggat ttcctgcacg gtgccctgga gcgagacaag aaggatgggt tccagcagac
     2101 ctcagagcat gccctattct cctgctccgt ggtggatgtc ttctcccaac tcaaccagag
     2161 ctttgaaatc atcaagaaac tcgagtgtcc cgaccctcag atcgtggggc actacatgag
     2221 gcgctttgcc aagaccatca gtaatgtgct cctccagtat gcagacatca tctccaagga
     2281 ctttgcctcc tactgctcca aggagaagga gaaagtgccc tgcattctca tgaataacac
     2341 tcaacagcta cgagttcagc tggagaagat gttcgaagcc atgggaggaa aggagctgga
     2401 tgctgaagcc agtgacatcc tgaaggagct tcaggtgaaa ctcaataacg tcttggatga
     2461 gctcagccgg gtgtttgcta ccagcttcca gccgcacatt gaagagtgtg tcaaacagat
     2521 gggtgacatc cttagccagg ttaagggcac aggcaatgtg ccagccagtg cctgcagcag
     2581 cgtggcccag gacgcggaca atgtgttgca gcccatcatg gacctgctgg acagcaacct
     2641 gaccctcttt gccaaaatct gtgagaagac tgtgctgaag cgagtgctga aggagctgtg
     2701 gaagctggtt atgaacacca tggagaaaac catcgtcctg ccgcccctca ctgaccagac
     2761 gatgatcggg aacctcttga gaaaacatgg caagggatta gaaaagggca gggtgaaatt
     2821 gccaagccac tcagacggaa cccagatgat cttcaatgca gccaaggagc tgggtcagct
     2881 gtccaaactc aaggatcaca tggtacgaga agaagccaag agcttgaccc caaagcagtg
     2941 cgcggttgtt gagttggccc tggacaccat caagcaatat ttccacgcgg gtggcgtggg
     3001 cctcaagaag accttcctgg agaagagccc ggacctgcaa tccttgcgct atgccctgtc
     3061 gctctacacg caggccaccg acctgctaat caagaccttt gtacagacgc aatcggccca
     3121 gggcttgggt gtagaagacc ctgtgggtga agtctctgtc catgttgagc tgttcactca
     3181 tccaggaact ggggaacaca aggtcacagt gaaagtggtg gctgccaatg acctcaagtg
     3241 gcagacttct ggcatcttcc ggccgttcat cgaggtcaac atcattgggc cccagctcag
     3301 cgacaagaaa cgcaagtttg cgaccaaatc caagaacaat agctgggctc ccaagtacaa
     3361 tgagagcttc cagttcacgc tgagcgccga cgcgggtccc gagtgctatg agctgcaggt
     3421 gtgcgtcaag gactactgct tcgcgcgcga ggaccgcacg gtggggctgg ccgtgctgca
     3481 gctgcgtgag ctggcccagc gcgggagcgc cgcctgctgg ctgccgctcg gccgccgcat
     3541 ccacatggac gacacgggcc tcacggtgct gcgaatcctc tcgcagcgca gcaacgacga
     3601 ggtggccaag gagttcgtga agctcaagtc ggacacgcgc tccgccgagg agggcggtgc
     3661 cgcgcctgcg ccttgtacag actacaaaga ccatgacggt gattataaag atcatgacat
     3721 cgactacaag gatgacgatg acaagtaact cgagataatc aacctctgga ttacaaaatt
     3781 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     3841 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     3901 tataaatcct ggttagttct tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     3961 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgtttatttg tgaaatttgt
     4021 gatgctattg ctttatttgt aaccatctag ctttatttgt gaaatttgtg atgctattgc
     4081 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     4141 tatgtttcag gttcaggggg agatgtggga ggttttttaa agatctggag accggtgtgg
     4201 cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg
     4261 cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc
     4321 cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat
     4381 tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg
     4441 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     4501 cacttgccag cgccttagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     4561 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     4621 ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat
     4681 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     4741 tcttgttcca aactggaaca acactcaact ctatctcggg ctattctttt gatttataag
     4801 ggattttgcc gatttcggtc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     4861 cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct
     4921 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     4981 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     5041 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     5101 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     5161 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     5221 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     5281 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     5341 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     5401 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     5461 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     5521 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     5581 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     5641 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     5701 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     5761 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     5821 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     5881 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     5941 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     6001 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     6061 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     6121 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     6181 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     6241 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     6301 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     6361 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     6421 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     6481 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     6541 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     6601 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     6661 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     6721 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     6781 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     6841 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     6901 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
//