| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-TANC2-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001424 |
| 产品用途: | 3' AAVLINK plasmid for TANC2 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | TANC2 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-TANC2-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-TANC2-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-TANC2-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-TANC2-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-TANC2-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-TANC2-FLAG 7475 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7475)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 7, 2026 from SnapGene 6.0.2
https:///www.biofeng.com
FEATURES Location/Qualifiers
source 1..7475
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..4180
/codon_start=1
/label=TANC2
/translation="VPPLLLWTKKD*QPSAGLV*RAISQ*YVLWWITELPQTMLTRMAV
PHWIWQLSMAMLRWSSSW*IMGP*SSTLTTVECALWIGQWGAGTLLLLSLF*RKEPR*V
VRRYRVAHEVQPHGRWPPPSQTS*SSC*AS*WKRGTCFIRKVK*RKLPSATSTP*RSSL
EKGLVRT*KLSGN*RCLSSSTSLGVAGK*TILEWRRNLLLRPWS*NRNLMKLTMREQGQ
NAAADSSQQP*RT*TRPSSCVPTTVRSRDFC*EWKKSVDRCSSHSSHRRHRSLSSSCRK
KQNLSHSMKTYTLYRIYSRRSTWNRMLKMFPLASRQRPGPARGSRSSRAHPPLPRIGTQ
PTSPAHLLALIRFLTSGPVVL*ALPLDRPISPPHLPFLQLIRTHITGLAHHTLPRLIRE
DLTVSAPLLWEDRAKNTQALPLPLSGEALSIGPALQLKV*VSIDPSLVHPCAISRKQAS
VSFLADPNLHYPKWPSGPTRCLSSLWQFPSKGSGYSLPRPRL*EVTSPAQPSIQAPSSP
QEPMAK*PIQWPVNTSLHKET*ESARAGWFIKGQLGES*GMEGRCSMSKPA*VQAPSVS
MED*PKRIFHSDLPQHTEVA*DTARHHRSDAASQHPITQSVTQN*IWSAPPAN*VPLMC
RI*SEDLSVSTLTKSNRTRQLPGRCCIPKV*AFASLHLAIVSPPPPT*LRPSGHLLPSS
KWRSH*NLHMRGHVTSCRQCLQLKEVTPVSPPDPGPHHSWGS*IKQHGLSSTPTSTSRI
GPGQCHLWTPSSVPRLQATCLSLSPSVHHHPSATLPFITKPTMHRMAICWRTIITAPMG
CWLTGLVETSWSESARPPPIPT*R*LGLYLWLRHTRTTCTGSCPETLGKGRHPLSNQRD
RSWSLML"
misc_feature 2088..2093
/label=SDS
misc_feature 2444..2449
/label=SDS
misc_feature 2540..2545
/label=SDS
misc_feature 2764..2769
/label=SDS
misc_feature 3699..3704
/label=SDS
misc_feature 4031..4036
/label=SDS
misc_feature 4034..4039
/label=SDS
CDS 4183..4248
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 4264..4695
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 4408..4413
/label=SDS
repeat_region 4738..4878
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4738..4867
/label=AAV2 ITR
rep_origin 4953..5408
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5690..5794
/gene="bla"
/label=AmpR promoter
CDS 5795..6655
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6826..7414
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtgc ctccattgct
1561 cttatggaca aagaaggatt gacagccctc agctgggctt gtttgaaggg ccatctctca
1621 gtagtacgtt ctctggtgga taacggagct gccacagacc atgctgacaa gaatggccgt
1681 accccactgg atctggcagc tttctatggc gatgctgagg tggtccagtt cctggtagat
1741 catggggcca tgatcgagca cgttgactac agtggaatgc gccctttgga tagggcagtg
1801 gggtgccgga acacttctgt tgttgtcact cttctgaaga aaggagccaa gataggttgt
1861 cagacgttac cgagtcgccc acgaggtcca gccacatggg cgatggccac ctccaagcca
1921 gacatcatga tcatcctgtt gagcaagctg atggaagagg gggacatgtt ttataagaaa
1981 ggtaaagtaa aggaagctgc ccagcgctac cagtacgccc tgaagaagtt ccctagagaa
2041 gggtttggtg aggacttgaa aactttccgg gaactaaagg tgtctctcct cctcaacctc
2101 tctcggtgtc gcaggaaaat gaacgatttt ggaatggcgg aggaatttgc tactaaggcc
2161 ctggagctga aaccgaaatc ttatgaagct tactatgcga gagcaagggc aaaacgcagc
2221 agcagacagt tcgcagcagc cttagaggac ctgaacgagg ccatcaagct gtgtcccaac
2281 aaccgtgaga tccagagact tctgctgaga gtggaagaag agtgtagaca gatgcagcag
2341 ccacagcagc caccgccgcc accgcagcct cagcagcagt tgccggaaga agcagaacct
2401 gagccacagc atgaagacat atactctgta caggatatat tcgaggagga gtacctggaa
2461 caggatgttg aaaatgtttc cattggcctc cagacagagg cccggcccag ccaggggctc
2521 ccggtcatcc agagcccacc ctcctctccc ccgcatcggg actcagccta catctccagc
2581 tcacctcttg gctctcatca ggtttttgac ttccggtcca gtagttctgt aggctctccc
2641 actagacaga cctatcagtc cacctcacct gccctttctc caactcatca gaactcacat
2701 tacaggccta gcccaccaca cacttccccg gctcatcagg gaggatctta ccgtttcagc
2761 ccccctcctg tgggaggaca gggcaaagaa tacccaagcc ctcccccttc ccctctccgg
2821 agaggccctc agtatcgggc cagccctcca gctgaaagta tgagtgtcta tagatcccag
2881 tctggttcac ccgtgcgcta tcagcaggaa acaagcgtca gtcagcttcc tggcagaccc
2941 aaatctccat tatccaaaat ggcccagcgg ccctaccaga tgcctcagct ccctgtggca
3001 gttccccagc aagggctcag gctacagcct gccaaggccc agattgtgag aagtaaccag
3061 cccagcccag ccgtccattc aagcaccgtc atccccacag gagcctatgg ccaagtagcc
3121 cattcaatgg ccagtaaata ccagtcttca caaggagaca taggagtcag ccagagccgg
3181 ttggtttatc aagggtcaat tgggggaatc gtaggggatg gaaggccggt gcagcatgtc
3241 caagccagcc tgagtgcagg cgccatctgt cagcatggag gattgaccaa agaggatctt
3301 ccacagcgac cttcctcagc ataccgaggt ggcgtgagat acagccagac accacagatc
3361 ggacgcagcc agtcagcatc ctattaccca gtctgtcact caaaactaga tctggagcgc
3421 tcctccagcc aactaggttc ccctgatgtg tcgcatttaa tcagaagacc tatcagtgtc
3481 aaccctaacg aaatcaaacc gcacccgcca actcccaggc cgttgctgca ttcccaaagt
3541 gtaggccttc gcttctctcc atctagcaat agtatctcct ccacctccaa cctaactccg
3601 accttccggc catcttcttc catccagcaa atggagatcc cactgaaacc tgcatatgag
3661 aggtcatgtg acgagctgtc gccagtgtct ccaactcaag gaggttaccc cagtgagccc
3721 acccgatcca ggaccacacc attcatgggg atcatagata aaacagcacg gactcagcag
3781 tacccccacc tccaccagca gaatcggacc tgggcagtgt catctgtgga caccgtcctc
3841 agtcccacgt ctccaggcaa cctgcctcag cctgagtcct tcagtccacc atcatccatc
3901 agcaacattg ccttttataa caaaaccaac aatgcacaga atggccattt gctggaggac
3961 gattattaca gcccccatgg gatgctggct aacgggtctc gtggagacct cttggagcga
4021 gtcagccagg cctcctccta tcccgacgtg aaggtagctc ggactctacc tgtggctcag
4081 gcataccagg acaacctgta caggcagctg tcccgagact ctcggcaagg gcagacatcc
4141 cctatcaaac caaagagacc gttcgtggag tctaatgtta aggactacaa agaccatgac
4201 ggtgattata aagatcatga catcgactac aaggatgacg atgacaagta agaattctga
4261 tgactcgaga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
4321 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
4381 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggtta gttcttgcca
4441 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
4501 ctgacaattc cgtggtgttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
4561 tctagcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
4621 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg
4681 tgggaggttt tttaaagatc tggagaccgg tgtggcacgt gcggaccgag cggccgcagg
4741 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg
4801 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag
4861 cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
4921 tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc
4981 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct tagcgcccgc
5041 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
5101 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
5161 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
5221 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
5281 caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggtctattg
5341 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
5401 tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
5461 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
5521 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
5581 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
5641 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
5701 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
5761 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
5821 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
5881 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
5941 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
6001 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
6061 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
6121 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
6181 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
6241 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
6301 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
6361 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
6421 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
6481 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
6541 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
6601 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
6661 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
6721 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
6781 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
6841 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
6901 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
6961 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
7021 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
7081 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
7141 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
7201 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
7261 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
7321 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
7381 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
7441 gttcctggcc ttttgctggc cttttgctca catgt
//
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-TANC2-FLAG载体图谱质粒图谱pdf版和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-TANC2-FLAG载体序列质粒序列等相关资料下载
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-TANC2-FLAG质粒载体应用举例
- 上一篇：pAAVLINKv1-pCALM1-Cre-3'-TANC2-FLAG
- 下一篇：没有了
