| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SHANK2-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001412 |
| 产品用途: | 3' AAVLINK plasmid for SHANK2 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: |
SHANK2
|
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SHANK2-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SHANK2-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SHANK2-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SHANK2-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SHANK2-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SHANK2-FLAG 7233 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7233)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 6, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7233
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3946
/codon_start=1
/label=SHANK2
/translation="GSSMEIDPQAPEPPSQLRPDESLTVSSPFAAAIAGAVRDREKRLE
ARRNSPAFLSTDLGDEDVGLGPPAPRTRPSMFPEEGDFADEDSAEQLSSPMPSATPREP
ENHFVGGAEASAPGEAGRPLNSTSKAQGPESSPAVPSASSGTAGPGNYVHPLTGRLLDP
SSPLALALSARDRAMKESQQGPKGEAPKADLNKPLYIDTKMRPSLDAGFPTVTRQNTRG
PLRRQETENKYETDLGRDRKGDDKKNMLIDIMDTSQQKSAGLLMVHTVDATKLDNALQE
EDEKAEVEMKPDSSPSEVPEGVSETEGALQISAAPEPTTVPGRTIVAVGSMEEAVILPF
RIPPPPLASVDLDEDFIFTEPLPPPLEFANSFDIPDDRAASVPALSDLVKQKKSDTPQS
PSLNSSQPTNSADSKKPASLSNCLPASFLPPPESFDAVADSGIEEVDSRSSSDHHLETT
STISTVSSISTLSSEGGENVDTCTVYADGQAFMVDKPPVPPKPKMKPIIHKSNALYQDA
LVEEDVDSFVIPPPAPPPPPGSAQPGMAKVLQPRTSKLWGDVTEIKSPILSGPKANVIS
ELNSILQQMNREKLAKPGEGLDSPMGAKSASLAPRSPEIMSTISGTRSTTVTFTVRPGT
SQPITLQSRPPDYESRTSGTRRAPSPVVSPTEMNKETLPAPLSAATASPSPALSDVFSL
PSQPPSGDLFGLNPAGRSRSPSPSILQQPISNKPFTTKPVHLWTKPDVADWLESLNLGE
HKEAFMDNEIDGSHLPNLQKEDLIDLGVTRVGHRMNIERALKQLLDR"
misc_feature 1685..1690
/label=SDS
misc_feature 1782..1787
/label=SDS
misc_feature 2573..2578
/label=SDS
misc_feature 2633..2638
/label=SDS
misc_feature 2874..2879
/label=SDS
misc_feature 3038..3043
/label=SDS
misc_feature 3864..3869
/label=SDS
CDS 3953..4018
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 4022..4453
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 4166..4171
/label=SDS
repeat_region 4496..4636
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4496..4625
/label=AAV2 ITR
rep_origin 4711..5166
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5448..5552
/gene="bla"
/label=AmpR promoter
CDS 5553..6413
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6584..7172
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacagggca gcagcatgga
1561 gatcgacccc caggccccgg agccaccgag ccagctgcgg cctgacgaaa gcctgaccgt
1621 cagcagcccc tttgccgccg ccatcgccgg agccgtccgc gaccgtgaga agcggctgga
1681 agccaggagg aactccccgg ccttcctctc cacagacctg ggggatgagg atgtgggcct
1741 ggggccaccc gcccccagga cgcggccctc catgttcccc gaggaggggg attttgctga
1801 cgaggacagc gctgagcagc tgtcatcccc catgccgagt gccacgccca gggagcccga
1861 aaaccatttc gtgggtggcg ccgaggccag tgctccgggt gaggctggga ggccgctgaa
1921 ttccacgtcc aaagcccagg ggcccgagag cagcccagca gtgccctccg cgagcagcgg
1981 cacagccggc cccgggaatt atgtccaccc actcacaggg cggctgcttg atcccagctc
2041 cccgctggcc ctggcactct ccgcaaggga ccgagccatg aaggagtctc aacagggacc
2101 caaaggggag gcccccaagg ccgacctcaa caaacctctt tacattgata ccaaaatgcg
2161 gcccagcctg gatgccggct tccctacggt caccaggcag aacacccggg gacccctgag
2221 gcggcaggag acggagaaca agtacgagac cgacctgggc cgagaccgga aaggcgatga
2281 caagaagaac atgctgatcg acatcatgga cacgtcccag cagaagtcgg ctggcctgct
2341 gatggtgcac accgtggacg ccactaagct ggacaacgcc ctgcaggaag aggacgagaa
2401 ggcagaggtg gagatgaagc cagacagctc gccgtccgag gtgccagaag gtgtttccga
2461 aaccgaaggt gctttacaga tctccgctgc ccccgagccc accaccgtgc ccggcagaac
2521 catcgtcgcg gtgggctcca tggaagaggc ggtgattttg ccattccgca tccctcctcc
2581 ccctctggca tccgtggact tggatgagga ttttattttt acagagccat tgcctcctcc
2641 cctggaattt gcaaatagtt ttgatatccc cgatgaccgg gcagcttctg tcccggctct
2701 ctcagactta gtgaagcaga agaaaagcga cacccctcag tccccttcgt tgaactccag
2761 ccaaccaacc aactctgcag acagcaagaa gccagccagt ctttcaaact gtctgcctgc
2821 ctcattcctg ccaccccctg aaagctttga cgccgtcgcc gactctggga tcgaggaggt
2881 ggacagccgg agtagcagcg accaccacct cgagacgacc agcactatct ccaccgtgtc
2941 tagcatctcc accctgtctt ccgaaggtgg agagaatgtg gacacctgca cagtctatgc
3001 agatgggcaa gcatttatgg ttgacaaacc cccagtacct cctaagccaa aaatgaagcc
3061 catcattcac aaaagcaatg cactttatca agacgcgctc gtggaagaag atgtagatag
3121 ctttgttatc cccccgcccg ctcccccgcc cccgccgggc agtgcccagc ctgggatggc
3181 caaggttctc cagccaagga cctccaagtt gtggggcgac gtcacagaga tcaaaagccc
3241 gattctctca ggcccaaagg caaacgttat tagtgaattg aactctatcc tacagcaaat
3301 gaaccgagag aaattggcaa agccggggga aggactggat tcaccaatgg gagccaagtc
3361 cgccagcctc gctccaagaa gcccggagat catgagcacc atctcaggta cacggagcac
3421 gacggtcacc ttcactgttc gccccggcac ctcccagccc atcaccctgc agagccggcc
3481 ccccgactat gaaagcagga cctcaggaac aagacgtgcc ccaagccctg tggtctcgcc
3541 aacagagatg aacaaagaga ccctgcccgc ccccctgtct gctgccaccg cctctccttc
3601 tcccgctctc tcagatgtct ttagccttcc aagccagccc ccttctgggg atctatttgg
3661 cttgaaccca gcgggacgca gtaggtcgcc atccccctcg atactgcaac agccaatctc
3721 aaataagcct tttacaacta aacctgtcca cctgtggact aaaccagatg tggccgattg
3781 gctggaaagt ctaaacttgg gtgaacataa agaggccttc atggacaatg agatcgatgg
3841 cagtcactta ccaaacctgc agaaggagga cctcatcgat cttggggtaa ctcgagtcgg
3901 gcacagaatg aacatagaaa gggctttgaa acagctgctg gacagatgta cagactacaa
3961 agaccatgac ggtgattata aagatcatga catcgactac aaggatgacg atgacaagta
4021 actcgagata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
4081 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
4141 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttagt tcttgccacg
4201 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
4261 gacaattccg tggtgtttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatc
4321 tagctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
4381 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggagatgtg
4441 ggaggttttt taaagatctg gagaccggtg tggcacgtgc ggaccgagcg gccgcaggaa
4501 cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg
4561 cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg
4621 cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
4681 tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg
4741 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc
4801 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
4861 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
4921 ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
4981 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
5041 actctatctc gggctattct tttgatttat aagggatttt gccgatttcg gtctattggt
5101 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
5161 caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
5221 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
5281 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
5341 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
5401 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
5461 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
5521 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
5581 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
5641 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
5701 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
5761 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
5821 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
5881 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
5941 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
6001 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
6061 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
6121 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
6181 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
6241 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
6301 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
6361 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
6421 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
6481 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
6541 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
6601 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
6661 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
6721 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
6781 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
6841 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
6901 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
6961 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
7021 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
7081 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
7141 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
7201 tcctggcctt ttgctggcct tttgctcaca tgt
//
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pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SHANK2-FLAG质粒载体应用举例
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