| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PRR14L-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001433 |
| 产品用途: | 3' AAVLINK plasmid for PRR14L expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | PRR14L |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PRR14L-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PRR14L-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PRR14L-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PRR14L-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PRR14L-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PRR14L-FLAG 7272 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7272)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 7, 2026 from SnapGene 6.0.2
https:///www.biofeng.com
FEATURES Location/Qualifiers
source 1..7272
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3971
/codon_start=1
/label=PRR14L
/translation="GTTGLLNCWGAI*GVGYQRNWRWRSREQHLSDPF*MPGDT*SC*K
TAEP*GFGLHVAERREIYTSTKSTYDIATVHII*SVVR*CTKSEPTEG*QR*VHHDQ*N
HPSKAGQGQHCGTDSEVRRPEGGKVTSSIKEGH*VMHKSLPSWCPSESTRPLLCWV*SN
TWCLCEERSSSLKEAAPSNL*ESFLSGANHCWEKNR*NQKFCLFKEFFQSHPHKSAQTS
QFVYSVCTYTIRT*NSTYQELG*SHTKADVYTVPSLEEPEF*EDYQRISLTKQAVHPCL
QTGPSHEDSEAKIPTVFL*TSSNG*KLQAPQI*KTPGRIFIQHRAAESIFGS*WMG*EA
*Q*AHGTLFSRIHQDDLHRFEQQDAVPAVWF*NLPSILSCEIIQLRLHD*VLKDFS*AL
CSGKACLRRGPPVPVSTSQVDLFFLLVPRLPWDGNIQGRHWRP*SDPHSGSSPASSSSP
RLWRHCHSPDQSRLLCPWPSHTSSTLFPRMLPNLDKKTELLQPHAYHAEALHDPVYTGP
ERVTVSSLHSRQGLLFSALLGGQSPIHLEPAWSFCLLLRNLFSSFPSLQAATKSGHHKQ
PHHVTICASSRHGSYI*HQRQSDEAGASIPCLGTKVLLGSRISCQQAPAFSL*VPGSWI
G*AGWCESSMPLPTEQPPRTERG*AREEAKESLTDSHPENHS*ARS*SYPHGPSSTQKV
KEEGV*FRRDIYQQEL*ISSCKQVFRDHL*GTQGTKWYTNLNQPTEEEASSRISGFYSP
AKEESSRQSQGGRQLYQGPEGSCAESRAGCSFDTETNGTGDLLCQGRGAGTIIRL"
misc_feature 1921..1926
/label=SDS
misc_feature 2011..2016
/label=SDS
misc_feature 3684..3689
/label=SDS
CDS 3980..4045
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 4061..4492
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 4205..4210
/label=SDS
repeat_region 4535..4675
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4535..4664
/label=AAV2 ITR
rep_origin 4750..5205
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5487..5591
/gene="bla"
/label=AmpR promoter
CDS 5592..6452
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6623..7211
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacagggga cgactgggtt
1561 acttaactgt tggggagcaa tctgaggagt tggttaccag agaaactggc gatggcgatc
1621 ccgtgagcaa catctctcag acccatttta aatgccgggg gatacttaat catgctgaaa
1681 aacagcagag ccctgaggtt ttggactaca tgttgcagaa agaagagaaa tatatacgtc
1741 aacaaaaagc acatacgata tcgcaacagt gcatatcatc tagtctgttg ttagatgatg
1801 cacaaaatca gaaccaaccg aaggctgaca aagatgagtc caccatgatc aatgaaatca
1861 ccctagcaaa gctggccaag gacagcattg tggcacagac tcagaagtta gaagaccaga
1921 aggaggaaag gttacatcat ccattaagga aggacactga gtcatgcaca agtccttgcc
1981 ttcttggtgc ccctcggaaa gcacaagacc cctcctctgc tgggtgtgat caaatacatg
2041 gtgcctttgc gaagaaagga gttcttccct taaagaagca gccccatcga acttgtaaga
2101 aagtttccta tcaggagcaa atcattgttg ggagaaaaat aggtaaaatc agaagttctg
2161 cctttttaaa gagttcttcc aatcccatcc ccacaaaagc gcacagactt ctcagtttgt
2221 gtactctgtc tgcacctaca cgattagaac ctgaaacagc acctaccaag agcttggtta
2281 gtcacatacc aaagcagatg tctacaccgt gccatccctt gaggagcctg aattttagga
2341 agactaccaa agaatcagcc ttactaaaca agctgtccat ccttgcctcc aaactggccc
2401 cagccatgaa gactcagaag ctaagatacc gacggtgttc ctctgaactt cttccaatgg
2461 ctaaaagcta caagcgcctc agatataaaa gactcctgga cggattttca tccagcacag
2521 agcagctgaa tccatatttg gcagctagtg gatgggataa gaggcctaac agtaagccca
2581 tggcacttta ttctctcgaa tccatcaaga tgaccttcat agatttgagc aacaagatgc
2641 cgtccctgct gtttggttct gaaatcttcc cagtatcctt tcatgtgaaa tcatccagct
2701 cagattgcac gactgagtcc tcaaggactt ttcctgagca ctgtgctccg gcaaggcttg
2761 ccttaggaga ggccctccag tgcccgtctc aacctcccaa gtggaccttt tctttcttct
2821 tgtcccacgg ttgccctggg atggcaacat tcagggaaga cactggcgtc catagtcaga
2881 cccacactca ggctcctccc cagcctccag ctcctctcca agactatgga ggcactgcca
2941 tagtccagac cagagcagac tgctctgtcc ttggccttca cacacttcta gcactttgtt
3001 ccccaggatg ttaccgaatc tggacaaaaa aacggagctt ctccagccac atgcctacca
3061 tgcagaggct cttcatgacc cagtttacac agggcctgaa agggttacgg tctccagcct
3121 ccatagcaga caaggtcttc tgttctctgc cctactcggt gggcagagtc ctatccattt
3181 ggagccagca tggtccttct gtctgctcct tcgaaatctc ttctcttcat tcccctcact
3241 gcaagcggca accaagtctg ggcaccacaa gcagccacac catgttacca tatgtgcctc
3301 ttccaggcat ggaagctaca tataacacca gcggcagtca gacgaggctg gagcctccat
3361 tccctgcctt ggtaccaaag tcttgcttgg tagcagaatc agctgtcagc aagctcctgc
3421 tttcagcctc tgagttccag gttcgtggat tggatgagct ggatggtgtg aaagcagcat
3481 gcccctgccc acagagcagc cccccagaac agaaagaggc tgagccagag aagaggccaa
3541 agaaagtctc acagattcgc atccggaaaa ccattcctag gccagatcct aatcttaccc
3601 ccatgggcct tcctcgaccc aaaaggttaa agaagaagga gtttagttta gaagagatat
3661 ataccaacaa gaattataaa tctcctcctg caaacaggtg tttagagacc atctttgagg
3721 aacccaagga acgaaatggt acactaatct caatcagcca acagaagagg aagcgagttc
3781 tagaatttca ggattttaca gtcccgcgaa agaggagagc tcgaggcaaa gtcaaggtgg
3841 caggcagctt taccagggcc cagaaggcag ctgtgcagag tcgagagctg gatgctcttt
3901 tgatacagaa actaatggaa ctggagacct tctttgccaa ggaagaggag caggaacaat
3961 catcaggctg ttgtacaagg actacaaaga ccatgacggt gattataaag atcatgacat
4021 cgactacaag gatgacgatg acaagtaaga attctgatga ctcgagataa tcaacctctg
4081 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
4141 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
4201 ttctcctcct tgtataaatc ctggttagtt cttgccacgg cggaactcat cgccgcctgc
4261 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgtttatt
4321 tgtgaaattt gtgatgctat tgctttattt gtaaccatct agctttattt gtgaaatttg
4381 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
4441 ttgcattcat tttatgtttc aggttcaggg ggagatgtgg gaggtttttt aaagatctgg
4501 agaccggtgt ggcacgtgcg gaccgagcgg ccgcaggaac ccctagtgat ggagttggcc
4561 actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc
4621 ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc
4681 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc
4741 aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
4801 gcgtgaccgc tacacttgcc agcgccttag cgcccgctcc tttcgctttc ttcccttcct
4861 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
4921 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac
4981 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
5041 ttaatagtgg actcttgttc caaactggaa caacactcaa ctctatctcg ggctattctt
5101 ttgatttata agggattttg ccgatttcgg tctattggtt aaaaaatgag ctgatttaac
5161 aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca
5221 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
5281 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
5341 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
5401 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
5461 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
5521 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
5581 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
5641 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
5701 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
5761 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
5821 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
5881 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
5941 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
6001 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
6061 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
6121 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
6181 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
6241 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
6301 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
6361 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
6421 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
6481 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
6541 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
6601 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
6661 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
6721 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
6781 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
6841 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
6901 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
6961 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
7021 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
7081 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
7141 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
7201 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
7261 ttgctcacat gt
//
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pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PRR14L-FLAG质粒载体应用举例
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