| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PREX1-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001523 |
| 产品用途: | 3' AAVLINK plasmid for PREX1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | PREX1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PREX1-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PREX1-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PREX1-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PREX1-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PREX1-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PREX1-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PREX1-FLAG 6384 bp DNA circular SYN 17-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6384)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 14, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6384
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3085
/codon_start=1
/label=PREX1
/translation="VCFKVAEEDQEDSGHDTMSYRDSYSECNSNRDSVLSYTSVRSNSS
YLGSDEMGSGDELPCDMRIPSDKQDKLHGCLEHLFNQVDSINALLKGPVMSRAFEETKH
FPMNHSLQEFKQKEECTIRGRSLIQISIQEDPWNLPNSIKTLVDNIQRYVEDGKNQLLL
ALLKCTDTELQLRRDAIFCQALVAAVCTFSEQLLAALGYRYNNNGEYEESSRDASRKWL
EQVAATGVLLHCQSLLSPATVKEERTMLEDIWVTLSELDNVTFSFKQLDENYVANTNVF
YHIEGSRQALKVIFYLDSYHFSKLPSRLEGGASLRLHTALFTKVLENVEGLPSPGSQAA
EDLQQDINAQSLEKVQQYYRKLRAFYLERSNLPTDASTTAVKIDQLIRPINALDELCRL
MKSFVHPKPGAAGSVGAGLIPISSELCYRLGACQMVMCGTGMQRSTLSVSLEQAAILAR
SHGLLPKCIMQATDIMRKQGPRVEILAKNLRVKDQMPQGAPRLYRLCQPPVDGDL"
misc_feature 1566..1571
/label=SDS
misc_feature 1575..1580
/label=SDS
misc_feature 1944..1949
/label=SDS
misc_feature 2277..2282
/label=SDS
CDS 3092..3157
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3173..3604
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3317..3322
/label=SDS
repeat_region 3647..3787
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3647..3776
/label=AAV2 ITR
rep_origin 3862..4317
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4599..4703
/gene="bla"
/label=AmpR promoter
CDS 4704..5564
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5735..6323
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtgt gcttcaaggt
1561 ggccgaggag gaccaggagg actcaggcca cgacaccatg agttatcgcg actcctacag
1621 cgagtgtaac agcaatcgag actcggtcct gtcctacacc agcgtgagaa gtaacagctc
1681 ctacttgggc agcgacgaga tggggtctgg agatgagctg ccctgtgaca tgcggatccc
1741 atctgacaag caggacaagc ttcatggctg cctggagcac ctctttaacc aggtggactc
1801 catcaatgct ctcctcaagg ggccagtcat gagccgggct ttcgaagaga ccaagcattt
1861 ccctatgaac cacagcttac aagagtttaa acagaaagaa gagtgtacaa tccgtggccg
1921 gagcctgatc cagattagca tccaggagga cccctggaac ctccccaact ccatcaagac
1981 cctggtggac aacattcaga gatatgtgga agatgggaag aaccagctgc tcctggcctt
2041 gctgaagtgc acagacacgg agctgcagct gcgcagagac gcgatcttct gccaggccct
2101 ggtggccgcc gtgtgcacct tctccgagca gctgctggcg gccctgggct accgctacaa
2161 caacaatggc gagtacgagg agagcagccg cgacgccagc cgcaagtggc tggagcaggt
2221 ggcggccacg ggcgtcctgc tgcactgcca gtccctgctc tcgccagcca cagtgaagga
2281 ggaacggacc atgctggagg acatctgggt gacgctgtca gagctggaca atgtcacctt
2341 ctcctttaag cagctggacg agaactatgt ggccaacacc aacgtcttct accacattga
2401 gggcagccgg caggcgctga aggtcatctt ctacctcgac agctaccact tctccaagct
2461 gccctcccgc ctggagggtg gggccagcct gaggctgcac acagcgctgt tcacgaaagt
2521 gctggagaac gtggaggggc tgccttctcc aggcagccag gccgcggagg atttgcagca
2581 ggacatcaac gcgcagtccc tggagaaagt tcagcagtat taccgcaaac tcagggcatt
2641 ttacctggag cggtctaacc tgcccacgga tgccagcacc acggcggtaa agatagacca
2701 gctgatccgc cccatcaatg ccctggatga gctctgccgc ctcatgaagt cctttgtcca
2761 cccaaagcct ggtgctgctg ggagtgtggg cgccggcctc atccccatct cctcggagct
2821 ctgctaccgc ctgggggcct gccagatggt catgtgtggc acaggcatgc agaggagcac
2881 cctgagcgtg tccctggagc aggcggccat cttggcacgg agccacgggt tgctgcccaa
2941 gtgcatcatg caggccacgg acatcatgcg gaagcagggc ccaagggtgg agattctggc
3001 caaaaacctg cgagtcaagg accagatgcc ccagggtgct ccgcgcctct accgcctctg
3061 ccagccgccg gtggatgggg acctctgtac agactacaaa gaccatgacg gtgattataa
3121 agatcatgac atcgactaca aggatgacga tgacaagtaa gaattctgat gactcgagat
3181 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
3241 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
3301 atggctttca ttttctcctc cttgtataaa tcctggttag ttcttgccac ggcggaactc
3361 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
3421 gtggtgttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat ctagctttat
3481 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
3541 taacaacaac aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt
3601 ttaaagatct ggagaccggt gtggcacgtg cggaccgagc ggccgcagga acccctagtg
3661 atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag
3721 gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc
3781 ctgcaggggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
3841 atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
3901 tggttacgcg cagcgtgacc gctacacttg ccagcgcctt agcgcccgct cctttcgctt
3961 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
4021 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg
4081 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
4141 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aactctatct
4201 cgggctattc ttttgattta taagggattt tgccgatttc ggtctattgg ttaaaaaatg
4261 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat
4321 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
4381 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
4441 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
4501 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
4561 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
4621 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
4681 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
4741 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
4801 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
4861 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
4921 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
4981 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
5041 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
5101 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
5161 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
5221 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
5281 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
5341 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
5401 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
5461 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
5521 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
5581 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
5641 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
5701 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
5761 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
5821 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
5881 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
5941 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
6001 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
6061 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
6121 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
6181 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
6241 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
6301 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
6361 tttgctggcc ttttgctcac atgt
//
