| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-POGZ-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001439 |
| 产品用途: | 3' AAVLINK plasmid for POGZ expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | POGZ |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-POGZ-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-POGZ-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-POGZ-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-POGZ-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-POGZ-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-POGZ-FLAG 6233 bp DNA circular SYN 13-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6233)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 8, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6233
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..2934
/codon_start=1
/label=POGZ
/translation="GAQSPKNLS*HQVVVVVVELAKRSSCL*RSFE*YCLLYAAIQNRQ
LNTSEIPSDVFAVGFDVSRPPRGRI*RANI*ALRQKRNWLSGC*PSANNSYL*MRRPCS
RRPPK*DVLWKGGLRSPMSGLCVSCCGTT*LPMPGELWPTPYLRM*QRMQDSSLILYNG
RFTTRTYPCL*LWLLMRSLCSWIQRC*AVMIERRMPCRQWAQGNLGVM*S*PFWQMALS
FPPWFSTEGRWISLLTCQTPYC*RQRRVATVMTRSWSCGQLECGRSTQLASAAKACL*W
TVIALTCQKRYWLCLVPLALCLQWSQQAVAPKFSH*MYASKELSRTSCIKNGRNRLGKW
QILHVILMSCFSWCLSGWVKC*VSLGTVQS*FSAPSWWLVFCLAPMATLTHLQEMLTCR
RS*LPP*RSN*S*VGNILSLPLHDPDHLLKRQLSLKVFTSSLRVKVRPSLSMALKKLT*
I*WR"
misc_feature 2741..2746
/label=SDS
CDS 2941..3006
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3022..3453
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3166..3171
/label=SDS
repeat_region 3496..3636
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3496..3625
/label=AAV2 ITR
rep_origin 3711..4166
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4448..4552
/gene="bla"
/label=AmpR promoter
CDS 4553..5413
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5584..6172
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacagggag cccagtcacc
1561 caagaacctg agctagcatc aggtggtggt ggtagtggtg gagttggcaa aaaggagcag
1621 ctgtctgtga agaagcttcg agtagtactg tttgctctat gctgcaatac agaacaggca
1681 gctgaacact tccgaaatcc ccagcgacgt attcgccgtt ggcttcgacg tttccaggcc
1741 tcccaggggg agaatctaga gggcaaatat ctgagctttg aggcagaaga gaaactggct
1801 gagtgggtgc taacccagcg cgaacaacag ctacctgtaa atgaggagac cttgttccag
1861 aaggccacca aaataggacg ttctttggaa ggggggttta agatctccta tgagtgggct
1921 gtgcgtttca tgctgcggca ccacctgact ccccatgccc ggcgagctgt ggcccacacc
1981 ctacctaagg atgtagcaga gaatgcagga ctcttcattg attttgtaca acggcagatt
2041 cacaaccagg acttaccctt gtctatgatt gtggctattg atgagatctc tttgttcctg
2101 gatacagagg tgctgagcag tgatgatcga aaggagaatg ccctgcagac agtgggcaca
2161 ggggaacctt ggtgtgatgt agtcctagcc attctggcag atggcactgt ccttcccacc
2221 ctggttttct acagagggca gatggatcag cctgctaaca tgccagactc catattgcta
2281 gaggcaaagg agagtggcta cagtgatgac gagatcatgg agctgtggtc aactcgagtg
2341 tggcagaagc acacagcttg ccagcgcagc aaaggcatgc ttgtgatgga ctgtcatcgc
2401 actcacttgt cagaagaggt actggctatg cttagtgcct ctagcacttt gcctgcagtg
2461 gtcccagcag gctgtagctc caaaattcag ccattagatg tatgcatcaa aagaactgtc
2521 aagaacttcc tgcataaaaa atggaaggaa caggctcggg aaatggcaga tactgcatgt
2581 gattctgatg tcctgcttca gctggtgctt gtctggctgg gtgaagtgct aggtgtcatt
2641 ggggactgtc cagagctagt tcagcgctcc ttcctggtgg ctagtgttct gcctggcccc
2701 gatggcaaca ttaactcacc tacaagaaat gctgacatgc aggaggagct aattgcctcc
2761 ctagaggagc aactgaagct gagtggggaa cattctgagt cttccactcc acgacccaga
2821 tcatctcctg aagagacaat tgagcctgaa agtcttcacc agctctttga gggtgaaagt
2881 gagaccgagt ctttctatgg ctttgaagaa gctgacctag atctgatgga gatttgtaca
2941 gactacaaag accatgacgg tgattataaa gatcatgaca tcgactacaa ggatgacgat
3001 gacaagtaag aattctgatg actcgagata atcaacctct ggattacaaa atttgtgaaa
3061 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
3121 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
3181 cctggttagt tcttgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
3241 gggctcggct gttgggcact gacaattccg tggtgtttat ttgtgaaatt tgtgatgcta
3301 ttgctttatt tgtaaccatc tagctttatt tgtgaaattt gtgatgctat tgctttattt
3361 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
3421 caggttcagg gggagatgtg ggaggttttt taaagatctg gagaccggtg tggcacgtgc
3481 ggaccgagcg gccgcaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc
3541 gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg
3601 cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc
3661 ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg
3721 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
3781 cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
3841 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
3901 gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg
3961 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
4021 ccaaactgga acaacactca actctatctc gggctattct tttgatttat aagggatttt
4081 gccgatttcg gtctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
4141 taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc
4201 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
4261 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
4321 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
4381 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
4441 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
4501 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
4561 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
4621 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
4681 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
4741 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
4801 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
4861 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
4921 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
4981 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
5041 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
5101 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
5161 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
5221 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
5281 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
5341 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
5401 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
5461 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
5521 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
5581 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
5641 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
5701 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
5761 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
5821 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
5881 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
5941 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
6001 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
6061 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
6121 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
6181 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgt
//
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