| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PHRF1-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001520 |
| 产品用途: | 3' AAVLINK plasmid for PHRF1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | PHRF1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PHRF1-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PHRF1-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PHRF1-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PHRF1-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PHRF1-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PHRF1-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PHRF1-FLAG 6556 bp DNA circular SYN 12-JAN-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6556)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 14, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6556
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3269
/codon_start=1
/label=PHRF1
/translation="GAQAGPLGPQPEDVPVAVGEPWQLFL*AL*E*EEEEKEISVQTSG
KGVLPHQQPGEALQAQASAGTQPRAARQEGECGVAPRPEEAEVPVPKLGAQGTGAQAAS
VP*EVAADPVPFPREEGGCEGGFPSTPCTGGARAGRPPHQVASLGGSTCLAGGGYGRQG
PPAGSPCPGGGS*V*AGRPGPGLWRLRGGRTRL**FLKRRRFHPAR*HELATFSRKHRL
FPRARLPTEACVAPSQPGRGRHPEGGVIDAR*RPFAAPTPARGHPGATFAQAGRG*EG*
GTQFPGCGACGEGRQPLCEWEGTGGSPA*GGGFADPPAAVQSPGEAGHLEPAGVGEQRP
RRGQSPPGTTSQATEAPRRSLGHGGCGPHRGQAGVLRAALSQSRASGTRVPRHRPLSGL
QPRPAACPGPALKHPTLRTGQPAHGPVHPSGEPAASGLWGSTDPGPSARCPDPSLRASQ
SSHCSQQLGGEDPGPQASCGENQEGGVHEEAAHAGACCGGGEAGHQALLPEEGGDQGGV
QGHPAQGRAEDLPQQEWRDQPREGGQPGEGVRGQVQAHAQAQETRGRGGAAHAGGRG"
misc_feature 2458..2463
/label=SDS
misc_feature 2476..2481
/label=SDS
misc_feature 2992..2997
/label=SDS
misc_feature 3037..3042
/label=SDS
misc_feature 3088..3093
/label=SDS
CDS 3276..3341
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3345..3776
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3489..3494
/label=SDS
repeat_region 3819..3959
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3819..3948
/label=AAV2 ITR
rep_origin 4034..4489
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4771..4875
/gene="bla"
/label=AmpR promoter
CDS 4876..5736
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5907..6495
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacagggag cacaggcggg
1561 gcccctgggg ccacagccgg aggacgtccc ggtcgcggtc ggggagccct ggcagctctt
1621 cctatgagca ctatgagagt aggaagaaga agaaaaggag atcagcgtcc agacctcggg
1681 gaagggagtg ctcccccacc agcagcctgg agaggctctg caggcacaag catcagcggg
1741 aacgcagcca cgagcggcca gacaggaagg agagtgtggc gtggccccga gaccggagga
1801 agcggaggtc ccggtcccca agctcggagc acagggcacg ggagcacagg cggcctcggt
1861 cccgtgagaa gtggccgcag acccggtccc attccccaga gaggaagggg gctgtgaggg
1921 aggcttcccc agcacccctt gcacaggggg agccagggcg ggaagacctc cccaccaggt
1981 tgccagcctt gggggaagca catgtctcgc cggaggtggc tacggccgac aaggcccccc
2041 tgcaggctcc ccctgtcctg gaggtggcag ctgagtgtga gccggacgac ctggacctgg
2101 attatggcga ctccgtggag gccggacacg tctttgatga tttctcaagc gacgccgttt
2161 tcatccagct cgatgacatg agctcgccac cttctcccga aagcacagac tcttccccag
2221 agcgagactt cccactgaag cctgcgttgc ccccagccag cctggccgtg gccgccatcc
2281 agagggaggt gtcattgatg cacgatgaag acccttcgca gcccccaccc ctgccagagg
2341 gcacccagga gccacatttg ctcaggccgg acgcggctga gaaggctgag gcacccagtt
2401 ccccggatgt ggcgcctgcg gggaaggaag acagcccctc tgcgagtggg agggtacagg
2461 aggcagcccg gcctgaggag gtggtttcgc agacccccct gctgcggtcc agagccctgg
2521 tgaagcgggt cacctggaac ctgcaggagt cggagagcag cgcccccgcc gaggacagag
2581 ccccccgggc accacttcac aggccacaga agccccgaga aggagcctgg gacatggagg
2641 atgtggcccc cacaggggtc aggcaggcgt tctccgagct gccctttccc agtcacgtgc
2701 ttccggaacc cgggttccca gacacagacc cctctcaggt ttacagcccc ggcctgccgc
2761 ctgccccggc ccagccctca agcatcccac cctgcgcact ggtcagccag cccacggtcc
2821 agttcatcct tcaggggagc ctgccgctag tgggctgtgg ggcagcacag accctggccc
2881 cagtgcccgc tgccctgacc ccagcctcag agccagccag tcaagccact gcagccagca
2941 actcggagga gaagaccccg gcccccaggc tagctgcgga gaaaaccaag aaggaggagt
3001 acatgaagaa gctgcacatg caggagcgtg ctgtggagga ggtgaagctg gccatcaagc
3061 ccttctacca gaagagggag gtgaccaagg aggagtacaa ggacatcctg cgcaaggccg
3121 tgcagaagat ctgccacagc aagagtggag agatcaaccc cgtgaaggtg gccaacctgg
3181 tgaaggcgta cgtggacaag tacaggcaca tgcgcaggca caagaaacca gaggccgggg
3241 aggagccgcc cacgcagggg gccgagggct gtacagacta caaagaccat gacggtgatt
3301 ataaagatca tgacatcgac tacaaggatg acgatgacaa gtaactcgag ataatcaacc
3361 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
3421 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
3481 cattttctcc tccttgtata aatcctggtt agttcttgcc acggcggaac tcatcgccgc
3541 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
3601 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc atctagcttt atttgtgaaa
3661 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
3721 acaattgcat tcattttatg tttcaggttc agggggagat gtgggaggtt ttttaaagat
3781 ctggagaccg gtgtggcacg tgcggaccga gcggccgcag gaacccctag tgatggagtt
3841 ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg
3901 acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg
3961 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca
4021 aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
4081 cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct
4141 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
4201 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt
4261 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
4321 ttctttaata gtggactctt gttccaaact ggaacaacac tcaactctat ctcgggctat
4381 tcttttgatt tataagggat tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt
4441 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact
4501 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
4561 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
4621 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
4681 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
4741 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
4801 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
4861 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
4921 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
4981 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
5041 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
5101 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
5161 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
5221 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
5281 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
5341 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
5401 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
5461 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
5521 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
5581 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
5641 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
5701 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
5761 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
5821 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
5881 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
5941 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
6001 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
6061 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
6121 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
6181 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
6241 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
6301 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
6361 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
6421 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
6481 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
6541 ccttttgctc acatgt
//
