| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-NRXN1-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001451 |
| 产品用途: | 3' AAVLINK plasmid for NRXN1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | NRXN1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-NRXN1-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-NRXN1-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-NRXN1-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-NRXN1-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-NRXN1-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-NRXN1-FLAG 7279 bp DNA circular SYN 14-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7279)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 8, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7279
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3992
/codon_start=1
/label=NRXN1
/translation="GWVEQICL*LFRNRLSWQVL*ERGNGFEL*WEHVYENSAPRSHAY
GG*GCFLTVPIPACIWHSDGNHF*RLC*HPPPGARRRTCETDGQSRLYQD*L*FQQRSR
DSFCWL*PQ**RVAHSACSSAWKKFKVNSG*PTGHDRSNGR*SY*AGVP*HRDWHHHRT
TVSFFCPLQLHWTPAELDI*WNGIH*PV*KWRHRLL*A*CQIWLQEHHSRSCHLQDQIE
LCCLSYLASLHFYASFFPVQDNIPRWINSI*QWGWK*LYCG*IS*RVLTLRV*FGKWC*
PHQRKLK*TSQ*QSVAQRDDIKGHQQPPHCKD*HKNHNANHRRSQELRPQE*LIYRRSS
*RNIQILTKTCTCQRRLSRLPGIS*FKWTASGPHLRCSFLQRTDRERM*RAQHNLPRGL
MFQSRCVLATMGWLQL*L*YDFLQWTTLQ*PWDDIYL*QRWWTNHV*VAS**PTQYTSR
QTGHRF*HCSERSRIGASGQFFRLG*LPRTAYTPGKNWS*V*CWDR*HRH*RIQCNH**
WEIPCSSFHEEWWQCHVAGGQLASDRALPCRKQ**RAPGDC*TANSISTWSSS**MATR
QRASAHNLQ*PSNHNNWRERAGPALPGPALWAVLQWLESSEYGSRKRCQHRHSGKCETG
W*SAFLYDN*VNSHCHAIRDVHINYGDYHDPGY*HSQKRKAPDKRTH*PDHR*HPCGLS
RVSQR**GH*PL*AELRWVSQPNPSRRQRAVSRLSRSDPGVQQHHGYGRWDSSRCRPVH
PYPPLCHVQVQKPG*RLIPCGRESKLHQ*LSTVQWGCCKGETTQQCEKLQQK*EKQG*R
VLC"
misc_feature 2555..2560
/label=SDS
misc_feature 2889..2894
/label=SDS
misc_feature 3812..3817
/label=SDS
CDS 3999..4064
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 4068..4499
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 4212..4217
/label=SDS
repeat_region 4542..4682
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4542..4671
/label=AAV2 ITR
rep_origin 4757..5212
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5494..5598
/gene="bla"
/label=AmpR promoter
CDS 5599..6459
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6630..7218
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcat gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacagggat gggtggaaca
1561 gatatgtctg tgattgttcc ggaacaggct atcttggcag gtcctgtgag agagaggcaa
1621 cggttttgag ctatgatggg agcatgttta tgaaaattca gctccccgta gtcatgcata
1681 cggaggctga ggatgtttcc ttacggttcc gatcccagcg tgcatatggc attctgatgg
1741 caaccacttc tagagactct gctgacaccc tccgcctgga gctagacgca ggacgtgtga
1801 aactgacggt caatctagat tgtatcagga ttaactgtaa ttccagcaaa ggtcccgaga
1861 ctctttttgc tggctataac ctcaatgata acgagtggca cacagtgcgt gtagttcggc
1921 gtggaaaaag tttaaagtta acagtggatg accaacaggc catgacaggt caaatggcag
1981 gtgatcatac taggctggag ttccataaca tagagactgg catcatcaca gaacgacggt
2041 atctttcttc tgtcccctcc aacttcattg gacacctgca gagcttgaca tttaatggaa
2101 tggcatacat tgacctgtgt aaaaatggcg acatagatta ctgtgagctt aatgccagat
2161 ttggcttcag gaacatcata gcagatcctg tcaccttcaa gaccaaatcg agctatgttg
2221 ccttagctac cttgcaagcc tacacttcta tgcatctttt tttccagttc aagacaacat
2281 ccctagatgg attaattcta tataacagtg gggatggaaa tgactttatt gtggttgaat
2341 tagttaaagg gtacttacat tacgtgtttg atttgggaaa tggtgctaac ctcatcaaag
2401 gaagctcaaa taaacctctc aatgacaatc agtggcacaa cgtgatgata tcaagggaca
2461 ccagcaacct ccacactgta aagattgaca caaaaatcac aacgcaaatc accgccggag
2521 ccaggaactt agacctcaag agtgacttat atataggagg agtagctaaa gaaacataca
2581 aatccttacc aaaacttgta catgccaaag aaggctttca aggctgcctg gcatcagttg
2641 atttaaatgg acggcttccg gacctcatct ccgatgctct tttctgcaac ggacagatcg
2701 agagaggatg tgaagggccc agcacaacct gccaagagga ctcatgttcc aatcaaggtg
2761 tgtgcttgca acaatgggat ggcttcagct gtgactgtag tatgacttcc ttcagtggac
2821 cactctgcaa tgaccctggg acgacatata tctttagcaa aggtggtgga caaatcacgt
2881 ataagtggcc tcctaatgac cgacccagta cacgagcaga cagactggcc ataggtttta
2941 gcactgttca gaaagaagcc gtattggtgc gagtggacag ttcttcaggc ttgggtgact
3001 acctagaact gcatatacac cagggaaaaa ttggagttaa gtttaatgtt gggacagatg
3061 acatcgccat tgaagaatcc aatgcaatca ttaatgatgg gaaataccat gtagttcgtt
3121 tcacgaggag tggtggcaat gccacgttgc aggtggacag ctggccagtg atcgagcgct
3181 accctgcagg aaacaatgat aacgagcgcc tggcgattgc tagacagcga attccatatc
3241 gacttggtcg agtagttgat gaatggctac tcgacaaagg gcgtcagctc acaatcttca
3301 atagccaagc aaccataata attggcggga aagagcaggg ccagcccttc cagggccagc
3361 tctctgggct gtactacaat ggcttgaaag ttctgaatat ggcagccgaa aacgatgcca
3421 acatcgccat agtgggaaat gtgagactgg ttggtgaagt gccttcctct atgacaactg
3481 agtcaacagc cactgccatg caatcagaga tgtccacatc aattatggag actaccacga
3541 ccctggctac tagcacagcc agaagaggaa agcccccgac aaaagaaccc attagccaga
3601 ccacagatga catccttgtg gcctcagcag agtgtcccag cgatgatgag gacattgacc
3661 cctgtgagcc gagctcaggt gggttagcca acccaacccg agcaggcggc agagagccgt
3721 atccaggctc agcagaagtg atccgggagt ccagcagcac cacgggtatg gtcgttggga
3781 tagtagccgc tgccgccctg tgcatcctta tcctcctcta tgccatgtac aagtacagaa
3841 accgggatga aggctcatac catgtggacg agagtcgaaa ctacatcagt aactcagcac
3901 agtccaatgg ggctgttgta aaggagaaac aacccagcag tgcgaaaagc tccaacaaaa
3961 ataagaaaaa caaggataaa gagtattatg tctgtacaga ctacaaagac catgacggtg
4021 attataaaga tcatgacatc gactacaagg atgacgatga caagtaactc gagataatca
4081 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
4141 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
4201 tttcattttc tcctccttgt ataaatcctg gttagttctt gccacggcgg aactcatcgc
4261 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
4321 gtttatttgt gaaatttgtg atgctattgc tttatttgta accatctagc tttatttgtg
4381 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
4441 acaacaattg cattcatttt atgtttcagg ttcaggggga gatgtgggag gttttttaaa
4501 gatctggaga ccggtgtggc acgtgcggac cgagcggccg caggaacccc tagtgatgga
4561 gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc
4621 ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gctgcctgca
4681 ggggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg
4741 tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
4801 acgcgcagcg tgaccgctac acttgccagc gccttagcgc ccgctccttt cgctttcttc
4861 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
4921 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat
4981 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
5041 acgttcttta atagtggact cttgttccaa actggaacaa cactcaactc tatctcgggc
5101 tattcttttg atttataagg gattttgccg atttcggtct attggttaaa aaatgagctg
5161 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc
5221 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
5281 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
5341 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
5401 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
5461 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
5521 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
5581 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
5641 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
5701 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
5761 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
5821 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
5881 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
5941 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
6001 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
6061 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
6121 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
6181 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
6241 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
6301 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
6361 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
6421 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
6481 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
6541 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
6601 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
6661 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
6721 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
6781 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
6841 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
6901 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
6961 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
7021 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
7081 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
7141 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
7201 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
7261 tggccttttg ctcacatgt
//
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