| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KIF14-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001517 |
| 产品用途: | 3' AAVLINK plasmid for KIF14 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | KIF14 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KIF14-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KIF14-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KIF14-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KIF14-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KIF14-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KIF14-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KIF14-FLAG 6717 bp DNA circular SYN 17-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6717)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 13, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6717
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3430
/codon_start=1
/label=KIF14
/translation="EIYVNKKRLEMETLATKQALEDHSIRHARILEALETEKQKIAKEV
QILQQNRNNRDKTFTVQTTWSSMKLSMMIQEANAISSKLKTYYVFGRHDISDKSSSDTS
IRVRNLKLGISTFWSLEKFESKLAAMKELYESNGSNRGEDAFCDPEDEWEPDITDAPVS
SLSRRRSRSLMKNRRISGCLHDIQVHPIKNLHSSHSSGLMDKSSTIYSNSAESFLPGIC
KELIGSSLDFFGQSYDEERTIADSLINSFLKIYNGLFAISKAHEEQDEESQDNLFSSDR
AIQSLTIQTACAFEQLVVLMKHWLSDLLPCTNIARLEDELRQEVKKLGGYLQLFLQGCC
LDISSMIKEAQKNAIQIVQQAVKYVGQLAVLKGSKLHFLENGNNKAASVQEEFMDAVCD
GVGLGMKILLDSGLEKAKELQHELFRQCTKNEVTKEMKTNAMGLIRSLENIFAESKIKS
FRRQVQEENFEYQDFKRMVNRAPEFLKLKHCLEKAIEIIISALKGCHSDINLLQTCVES
IRNLASDFYSDFSVPSTSVGSYESRVTHIVHQELESLAKSLLFCFESEESPDLLKPWET
YNQNTKEEHQQSKSSGIDGSKNKGVPKRVYELHGSSPAVSSEECTPSRIQWV"
misc_feature 2048..2053
/label=SDS
misc_feature 2715..2720
/label=SDS
CDS 3437..3502
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3506..3937
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3650..3655
/label=SDS
repeat_region 3980..4120
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3980..4109
/label=AAV2 ITR
rep_origin 4195..4650
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4932..5036
/gene="bla"
/label=AmpR promoter
CDS 5037..5897
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6068..6656
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggaaa tatatgtcaa
1561 caaaaagcga ttagaaatgg agacattggc tacaaaacag gctttagaag accatagcat
1621 ccgccatgca agaattctgg aagctttaga aactgaaaag caaaaaattg ctaaagaagt
1681 acaaattcta cagcagaatc ggaataatag ggataaaact tttacagtgc agacaacttg
1741 gagctctatg aaactctcaa tgatgattca ggaagccaat gctatcagca gcaaattgaa
1801 aacatactat gtttttggca gacatgatat atcagataaa agtagttctg acacttctat
1861 tcgggttcgt aacctgaaac taggaatctc aacattctgg agtctggaaa agtttgaatc
1921 taaacttgca gcaatgaaag aactttatga gagtaatggt agtaacaggg gtgaagatgc
1981 cttttgtgat cctgaagatg aatgggaacc cgacattaca gatgcaccag tttcttcact
2041 ttctagaagg aggagtagga gtttgatgaa gaacagaaga atttctggtt gtttacatga
2101 catacaagtc catccaatta agaatttgca ttcttcacat tcatcaggtt taatggacaa
2161 atcaagcact atttactcaa attcagcaga gtcctttctt cctggaattt gcaaagaatt
2221 gattggttct tcgttagatt tttttggaca gagttatgat gaagaaagaa ctatagcaga
2281 cagcctaatt aatagttttc ttaaaattta taatgggcta tttgccattt ccaaggctca
2341 tgaagaacaa gatgaagaaa gtcaagataa cttgttttct tctgatcgag caatccagtc
2401 acttactatt cagactgcat gtgcttttga gcagctagta gtgctaatga aacactggct
2461 gagtgattta ctgccttgta ccaacatagc aagacttgag gatgaattga gacaagaagt
2521 taaaaaactg ggaggctact tacagttatt tttgcaggga tgctgtttgg atatttcatc
2581 aatgataaaa gaggctcaaa agaatgcaat ccaaattgta caacaagctg taaagtatgt
2641 ggggcagtta gcagttctga aagggagcaa gctacatttt ctagaaaacg gtaacaataa
2701 agctgccagt gtccaggagg aattcatgga tgctgtttgt gatggtgtag gcttaggaat
2761 gaagatttta ttagattctg gactggaaaa agcaaaagaa cttcagcatg aactctttag
2821 gcagtgtaca aaaaatgagg ttaccaaaga aatgaaaact aatgccatgg gattgattag
2881 atctcttgaa aacatctttg ctgaatcgaa aattaaaagt ttcagaaggc aagtacaaga
2941 agaaaacttt gaataccaag atttcaagag gatggttaat cgtgctccag aattcttaaa
3001 gttaaaacat tgcttagaga aagctattga aattattatt tctgcactga aaggatgcca
3061 tagtgatata aatcttctcc agacttgtgt tgaaagtatt cgcaacttgg ccagtgattt
3121 ttacagtgac ttcagtgtgc cttctacttc tgttggcagc tatgagagta gagtaactca
3181 cattgtccac caggaactag aatctctagc taagtctctc ctcttttgtt ttgaatctga
3241 agaaagccct gatttgttga aaccctggga aacttataat caaaatacca aagaagaaca
3301 ccaacaatct aaatcaagcg ggattgacgg cagtaagaat aaaggtgtac caaagcgtgt
3361 ctatgagctc catggctcat ccccagcagt gagctcagag gaatgcacac ccagtaggat
3421 tcagtgggtg tgtacagact acaaagacca tgacggtgat tataaagatc atgacatcga
3481 ctacaaggat gacgatgaca agtaactcga gataatcaac ctctggatta caaaatttgt
3541 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
3601 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
3661 aaatcctggt tagttcttgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
3721 acaggggctc ggctgttggg cactgacaat tccgtggtgt ttatttgtga aatttgtgat
3781 gctattgctt tatttgtaac catctagctt tatttgtgaa atttgtgatg ctattgcttt
3841 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
3901 gtttcaggtt cagggggaga tgtgggaggt tttttaaaga tctggagacc ggtgtggcac
3961 gtgcggaccg agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc
4021 gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg
4081 gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt
4141 ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc
4201 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
4261 ttgccagcgc cttagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
4321 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
4381 tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc
4441 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
4501 tgttccaaac tggaacaaca ctcaactcta tctcgggcta ttcttttgat ttataaggga
4561 ttttgccgat ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
4621 attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg
4681 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
4741 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
4801 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
4861 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
4921 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
4981 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
5041 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
5101 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
5161 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
5221 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
5281 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
5341 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
5401 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
5461 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
5521 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
5581 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
5641 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
5701 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
5761 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
5821 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
5881 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
5941 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
6001 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
6061 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
6121 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
6181 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
6241 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
6301 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
6361 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
6421 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
6481 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
6541 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
6601 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
6661 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt
//
