| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KDM6B-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001391 |
| 产品用途: | 3' AAVLINK plasmid for KDM6B expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: |
SCP1
|
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | KDM6B |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KDM6B-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KDM6B-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KDM6B-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KDM6B-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KDM6B-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KDM6B-FLAG 6769 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6769)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 5, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6769
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3470
/codon_start=1
/label=KDM6B
/translation="GKGQGQGPQRKEPPGAGEPGPAERGDPGS*EVPARSWRGLQGQAT
HSSSPSISSCTFCPAHTPVSLCPWKEGSGGSPRATGCQPGRHAEAALT**GAPQGAEDP
AHQGREW*QGDLYRL*GGRAAAAHGRPHHQPLCC*RRARQQECQGEREVSRVLPFPCPV
CETEDQH*GEAAPGKTQPPYTQHLSGEQTGCLLTCPAAVLYRPSKSHHSDPGPGGLPAA
QLGPLLHQDPGGSEWRTHRGSSHPGAAALR*ELGSDRHSADLAL*ELPFPHHHCQVRTV
PGLILPGVSAGGEGE*G*GVRGARQHHWNPS*QRTRPEEPSHHQVWHQHRLV*C*AVEA
PAAGAAKAARLHAGNIHGQHAEPRGPHHPGHEHGAAVHEGARQPNARPPGE*QLLLRQH
QHWPRRLRVVRGARALLGDHQRFL*SARRGLLDGFLVANPG*SLCIQYSCVPLRAATRR
PRVD*CGDCALGAGHRLVQQHCLERGAPHRLSVPAGPGTIRVE*GEERQIHRAHDSRVM
ERGSHGQNQRPRLVQDDQVLPAAVHEALPGATREPGAGREENRLPGPCQGRASLLLQRV
RCGGV*HPVRDK*EWQPQHVPGTLRGLCPAPQRRPAGRGGAGAVPH*GAGSGLRRLHAG
PSQHVA"
misc_feature 2419..2424
/label=SDS
misc_feature 2478..2483
/label=SDS
CDS 3477..3542
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3558..3989
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3702..3707
/label=SDS
repeat_region 4032..4172
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4032..4161
/label=AAV2 ITR
rep_origin 4247..4702
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4984..5088
/gene="bla"
/label=AmpR promoter
CDS 5089..5949
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6120..6708
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacagggca aaggccaagg
1561 ccaaggtccc caaagaaaag agccgccggg tgctggggaa cctggacctg cagagcgagg
1621 agatccaggg tcgtgagaag tcccggcccg atcttggcgg ggcctccaag gccaagccac
1681 ccacagctcc agcccctcca tcagctcctg caccttctgc ccagcccaca cccccgtcag
1741 cctctgtccc tggaaagaag gctcgggagg aagccccagg gccaccgggt gtcagccggg
1801 ccgacatgct gaagctgcgc tcacttagtg aggggccccc caaggagctg aagatccggc
1861 tcatcaaggt agagagtggt gacaaggaga cctttatcgc ctctgaggtg gaagagcggc
1921 ggctgcgcat ggcagacctc accatcagcc actgtgctgc tgacgtcgtg cgcgccagca
1981 ggaatgccaa ggtgaaaggg aagtttcgag agtcctacct ttcccctgcc cagtctgtga
2041 aaccgaagat caacactgag gagaagctgc cccgggaaaa actcaacccc cctacaccca
2101 gcatctatct ggagagcaaa cgggatgcct tctcacctgt cctgctgcag ttctgtacag
2161 accctcgaaa tcccatcaca gtgatccggg gcctggcggg ctccctgcgg ctcaacttgg
2221 gcctcttctc caccaagacc ctggtggaag cgagtggcga acacaccgtg gaagttcgca
2281 cccaggtgca gcagccctca gatgagaact gggatctgac aggcactcgg cagatctggc
2341 cttgtgagag ctcccgttcc cacaccacca ttgccaagta cgcacagtac caggcctcat
2401 ccttccagga gtctctgcag gaggagaagg agagtgagga tgaggagtca gaggagccag
2461 acagcaccac tggaacccct cctagcagcg caccagaccc gaagaaccat cacatcatca
2521 agtttggcac caacatcgac ttgtctgatg ctaagcggtg gaagccccag ctgcaggagc
2581 tgctaaagct gcccgccttc atgcgggtaa catccacggg caacatgctg agccacgtgg
2641 gccacaccat cctgggcatg aacacggtgc agctgtacat gaaggtgccc ggcagccgaa
2701 cgccaggcca ccaggagaat aacaacttct gctccgtcaa catcaacatt ggcccaggcg
2761 actgcgagtg gttcgcggtg cacgagcact actgggagac catcagcgct ttctgtgatc
2821 ggcacggcgt ggactacttg acgggttcct ggtggccaat cctggatgat ctctatgcat
2881 ccaatattcc tgtgtaccgc ttcgtgcagc gacccggaga cctcgtgtgg attaatgcgg
2941 ggactgtgca ctgggtgcag gccaccggct ggtgcaacaa cattgcctgg aacgtggggc
3001 ccctcaccgc ctatcagtac cagctggccc tggaacgata cgagtggaat gaggtgaaga
3061 acgtcaaatc catcgtgccc atgattcacg tgtcatggaa cgtggctcgc acggtcaaaa
3121 tcagcgaccc cgacttgttc aagatgatca agttctgcct gctgcagtcc atgaagcact
3181 gccaggtgca acgcgagagc ctggtgcggg cagggaagaa aatcgcttac cagggccgtg
3241 tcaaggacga gccagcctac tactgcaacg agtgcgatgt ggaggtgttt aacatcctgt
3301 tcgtgacaag tgagaatggc agccgcaaca cgtacctggt acactgcgag ggctgtgccc
3361 ggcgccgcag cgcaggcctg cagggcgtgg tggtgctgga gcagtaccgc actgaggagc
3421 tggctcaggc ctacgacgcc ttcacgctgg ccccagccag cacgtcgcga tgtacagact
3481 acaaagacca tgacggtgat tataaagatc atgacatcga ctacaaggat gacgatgaca
3541 agtaagaatt ctgatgactc gagataatca acctctggat tacaaaattt gtgaaagatt
3601 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
3661 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
3721 gttagttctt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
3781 tcggctgttg ggcactgaca attccgtggt gtttatttgt gaaatttgtg atgctattgc
3841 tttatttgta accatctagc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
3901 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
3961 ttcaggggga gatgtgggag gttttttaaa gatctggaga ccggtgtggc acgtgcggac
4021 cgagcggccg caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc
4081 gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc
4141 agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac
4201 gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc
4261 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
4321 gccttagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
4381 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
4441 ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag
4501 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
4561 actggaacaa cactcaactc tatctcgggc tattcttttg atttataagg gattttgccg
4621 atttcggtct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
4681 aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca
4741 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
4801 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
4861 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
4921 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
4981 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
5041 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
5101 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
5161 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
5221 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
5281 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
5341 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
5401 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
5461 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
5521 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
5581 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
5641 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
5701 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
5761 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
5821 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
5881 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
5941 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
6001 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
6061 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
6121 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
6181 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
6241 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
6301 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
6361 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
6421 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
6481 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
6541 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
6601 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
6661 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
6721 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgt
//
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