| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KDM3B-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001406 |
| 产品用途: | 3' AAVLINK plasmid for KDM3B expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | KDM3B |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KDM3B-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KDM3B-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KDM3B-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KDM3B-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KDM3B-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KDM3B-FLAG 7172 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7172)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 6, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7172
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..115
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..115
/label=AAV2 ITR
misc_feature 130..210
/label=SCP1
CDS 222..1250
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1266..1355
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1280..1285
/label=SDS
misc_feature 1283..1288
/label=SDS
intron 1359..1399
/label=Splicing Donor
protein_bind 1406..1439
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1440..1531
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1532..3873
/codon_start=1
/label=KDM3B
/translation="G*I*RPQNTSWPMLGTSSASS*CLRRRP**WWSHTRKWHGSELCV
VYGRCVMCVKQLSSTSTGFVANVDLGSALTVTGSGKAGHAVRQKRWVMKKFSPG*SVQR
DSPTNQRISCPHKLFLAQLFTILETWYMLPGASGELKQTALVSVDRTNLY*DLPSPMGC
HSFLA*TLVPLLETKLPSLAEEDRHQ*QLQSRTMFPKPTALTSDLKSL*KQTVRHQIAI
VN*KPSGLLALTRPHPPPPCTGWQI*QLRRLKKKQKKQGP*GRCSIKSLIHPLGWTRST
PLQRSLR*LQSFLTVCCWVPLPPTTKPKGLAFETSFTPGRENFLKPPWTQAYPFPRSSL
HPQQE*RARPAYPTFLTTSLPQW*KIRKPQMLQSGPAT*LIPRRK*RRW*WG*MC*IPI
LLTPGFVMGGFCVSMTPATKTIGRSSGSVGSKVSQCWFRGYIKSSSLSSGSQKPLARNL
ETRM*TW*TAGTVL*FPM*KFGISGMVSRSYANDYGQKMGSQWCSNSRTGLLGKIFET*
CQPGLKI*WRTFLCQNIPNEMAGSIWPLGYLATL*GLIWAPRCTTPMG**QQKIEELVQ
QIFT*MCLMLLM*WCMLGFPSGRVLMMKRYSRQLTREMPMR*RSRGFMMEKRSQVLYGT
SMQPRMQRRSGSCSERLEKNKAKRTPLIMTQFMTKVGTWTRPSVSDSMRSMACKAGLLY
SS*VMLFSYLLEPHTRFTIYTVA*K*QKTLYLQNM*STVSA*LRNSGISLTLIQIMRIN
CR*RTSFTMQ*KMRLAPSRLMNPNWQG"
misc_feature 1607..1612
/label=SDS
misc_feature 2082..2087
/label=SDS
misc_feature 2218..2223
/label=SDS
misc_feature 2243..2248
/label=SDS
misc_feature 2475..2480
/label=SDS
misc_feature 3055..3060
/label=SDS
CDS 3880..3945
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3961..4392
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 4105..4110
/label=SDS
repeat_region 4435..4575
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4435..4564
/label=AAV2 ITR
rep_origin 4650..5105
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5387..5491
/gene="bla"
/label=AmpR promoter
CDS 5492..6352
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6523..7111
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgacctca
61 gtgagcgagc gagcgcgcag agagggagtg gccaactcca tcactagggg ttcctgcggc
121 cgcacgcgtg tacttatata agggggtggg ggcgcgttcg tcctcagtcg cgatcgaaca
181 ctcgagccga gcagacgtgc ctacggaccg cgtacgccac catgtccaat ttactgaccg
241 tacaccaaaa tttgcctgca ttaccggtcg atgcaacgag tgatgaggtt cgcaagaacc
301 tgatggacat gttcagggat cgccaggcgt tttctgagca tacctggaaa atgcttctgt
361 ccgtttgccg gtcgtgggcg gcatggtgca agttgaataa ccggaaatgg tttcccgcag
421 aacctgaaga tgttcgcgat tatcttctat atcttcaggc gcgcggtctg gcagtaaaaa
481 ccatccagca acatttgggc cagctaaaca tgcttcatcg tcggtccggg ctgccacgac
541 caagtgacag caatgctgtt tcactggtta tgcggcggat ccgaaaagaa aacgttgatg
601 ccggtgaacg tgcaaaacag gctctagcgt tcgaacgcac tgatttcgac caggttcgtt
661 cactcatgga aaatagcgat cgctgccagg atatacgtaa tctggcattt ctggggattg
721 cttataacac cctgttacgt atagccgaaa ttgccaggat cagggttaaa gatatctcac
781 gtactgacgg tgggagaatg ttaatccata ttggcagaac gaaaacgctg gttagcaccg
841 caggtgtaga gaaggcactt agcctggggg taactaaact ggtcgagcga tggatttccg
901 tctctggtgt agctgatgat ccgaataact acctgttttg ccgggtcaga aaaaatggtg
961 ttgccgcgcc atctgccacc agccagctat caactcgcgc cctggaaggg atttttgaag
1021 caactcatcg attgatttac ggcgctaagg atgactctgg tcagagatac ctggcctggt
1081 ctggacacag tgcccgtgtc ggagccgcgc gagatatggc ccgcgctgga gtttcaatac
1141 cggagatcat gcaagctggt ggctggacca atgtaaatat tgtaatgaac tatatccgta
1201 acctggatag tgaaacaggg gcaatggtgc gcctgctgga agatggcgat ggaggtggat
1261 ctggtctgct gctgaaactc ctcctcctcg gtggtggttc tggtcctcgt ccccgtggta
1321 ctcgtggtaa aggtcgccgt attcactaca aaggataagt aagtatcaag gttacaagac
1381 aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata gcaatttatg
1441 aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc tattggtctt
1501 actgacatcc actttgcctt tctctccaca gggatagatc tagagacctc aaaatacatc
1561 ctggccaatg ttggggacca gttctgccag ctcgtaatgt ctgagaagga ggccatgatg
1621 atggtggagc cacaccagaa agtggcatgg aagcgagctg tgcgtggtgt acgggagatg
1681 tgtgatgtgt gtgaaacaac tctcttcaac atccactggg tttgtcgcaa atgtggattt
1741 ggggtctgcc ttgactgtta ccggctcagg aaaagccggc cacgcagtga gacagaagag
1801 atgggtgatg aagaagtttt ctcctggttg aagtgtgcaa agggacagtc ccacgaacca
1861 gagaatctca tgcccacaca aattattcct ggcacagctc tttacaatat tggagacatg
1921 gtacatgctg cccggggcaa gtggggaatt aaagcaaact gcccttgtat cagtcgacag
1981 aacaaatctg tattgagacc tgccgtcacc aatgggatgt cacagcttcc tagcataaac
2041 cctagtgcct cttctggaaa cgaaactacc ttctctggcg gaggaggacc ggcaccagta
2101 acaactccag agccggacca tgttcccaaa gccgacagca ctgacatcag atctgaagag
2161 cctctgaaaa cagacagttc ggcatcaaat agcaatagtg aactgaaagc catcaggcct
2221 ccttgccctg acacggcccc accctcctcc gccctgcact ggttggcaga tttagcaact
2281 cagaaggcta aagaagaaac aaaagaagca gggtccctga ggtcggtgct caataaagag
2341 tctcattcac cctttgggct ggactcgttc aactccactg caaaggtctc tccgctgact
2401 ccaaagcttt ttaacagtct gttgctgggt cccactgcct ccaacaacaa aaccgaaggg
2461 tctagccttc gagacctcct tcactccggg ccgggaaaac ttcctcaaac ccccttggac
2521 acaggcatac cctttccccc ggtcttctct acatcctcag caggagtgaa gagcaaggcc
2581 agcctaccca actttcttga ccacatcatt gcctcagtgg tagaaaataa gaaaacctca
2641 gatgcttcaa agcgggcctg caacttgact gatacccaga aggaagtgaa ggagatggtg
2701 atggggttaa atgtgctaga tccccatact tctcactcct ggctttgtga tgggaggctt
2761 ctgtgtctcc atgaccccag caacaaaaac aattggaaga tcttccggga gtgttggaag
2821 caaggtcagc cagtgctggt ttcgggggta cataaaaagc tcaagtctga gctctggaag
2881 ccagaagcct ttagccagga atttggagac caggatgtag acttggtgaa ctgcaggaac
2941 tgtgctataa tttccgatgt gaaagttcgg gatttctggg atggtttcga gatcatatgc
3001 aaacgactac ggtcagaaga tgggcagcca atggtgctca aactcaagga ctggcctcct
3061 ggggaagatt ttcgagacat gatgccaacc aggtttgaag atctgatgga gaaccttcct
3121 ctgccagaat ataccaaacg agatggcagg ctcaatctgg cctctaggct acctagctac
3181 tttgtaaggc ctgatctggg ccccaagatg tacaacgcct atgggttgat aacagcagaa
3241 gatagaagag ttggtacaac aaatcttcac ttagatgtgt ctgatgctgt taatgtgatg
3301 gtgtatgttg ggattcccat cggggagggt gctcatgatg aagaggtact caagacaatt
3361 gacgagggag atgccgatga ggtgacgaag cagaggattc atgatggaaa agagaagcca
3421 ggtgctttat ggcacatcta tgcagccaag gatgcagaga agatccggga gctgctccga
3481 aaggttggag aagaacaagg ccaagagaac ccccctgatc atgacccaat tcatgaccaa
3541 agttggtacc tggaccagac cctccgtaag cgactctatg aggagtatgg cgtgcaaggc
3601 tgggctattg tacagttcct aggtgatgct gttttcatac ctgctggagc cccacaccag
3661 gttcacaatc tatacagttg cataaaagta gcagaagact ttgtatctcc agaacatgta
3721 aagcactgtt tccgcctgac tcaggaattc aggcatctct ctaacactca tacaaatcat
3781 gaggataaac tgcaggtgaa gaacatcatt taccatgcag tgaaagatgc ggttggcacc
3841 ctcaaggctc atgaatccaa actggcaagg tcctgtacag actacaaaga ccatgacggt
3901 gattataaag atcatgacat cgactacaag gatgacgatg acaagtaaga attctgatga
3961 ctcgagataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
4021 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
4081 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttagtt cttgccacgg
4141 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
4201 acaattccgt ggtgtttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatct
4261 agctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
4321 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggagatgtgg
4381 gaggtttttt aaagatctgg agaccggtgt ggcacgtgcg gaccgagcgg ccgcaggaac
4441 ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc
4501 gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc
4561 gcagctgcct gcaggggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
4621 cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc
4681 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccttag cgcccgctcc
4741 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
4801 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
4861 tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
4921 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
4981 ctctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg tctattggtt
5041 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac
5101 aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
5161 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
5221 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
5281 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
5341 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
5401 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
5461 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
5521 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
5581 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
5641 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
5701 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
5761 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
5821 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
5881 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
5941 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
6001 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
6061 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
6121 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
6181 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
6241 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
6301 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
6361 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
6421 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
6481 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
6541 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
6601 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
6661 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
6721 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
6781 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
6841 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
6901 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
6961 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
7021 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
7081 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
7141 cctggccttt tgctggcctt ttgctcacat gt
//
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pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KDM3B-FLAG质粒载体应用举例
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