| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ITSN1-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001544 |
| 产品用途: | 3' AAVLINK plasmid for ITSN1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | ITSN1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ITSN1-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ITSN1-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ITSN1-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ITSN1-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ITSN1-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ITSN1-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ITSN1-FLAG 6519 bp DNA circular SYN 17-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6519)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 16, 2026 from SnapGene 6.0.2
https:///www.biofeng.com
FEATURES Location/Qualifiers
source 1..6519
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3232
/codon_start=1
/label=ITSN1
/translation="VIGMYDYTAQNDDELAFNKGQIINVLNKEDPDWWKGEVNGQVGLF
PSNYVKLTTDMDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIF
QKPLMESELLTEKEVAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILSAQ
LPHMQPYIRFCSRQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQR
VTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHV
QCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQITKPLGSS
GTDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINE
RTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELKPCRSHGKS
NPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLG
RTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP"
misc_feature 1629..1634
/label=SDS
misc_feature 2527..2532
/label=SDS
CDS 3239..3304
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3308..3739
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3452..3457
/label=SDS
repeat_region 3782..3922
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3782..3911
/label=AAV2 ITR
rep_origin 3997..4452
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4734..4838
/gene="bla"
/label=AmpR promoter
CDS 4839..5699
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5870..6458
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtga ttgggatgta
1561 cgactacacc gcgcagaatg acgatgagct ggccttcaac aagggccaga tcatcaacgt
1621 cctcaacaag gaggaccctg actggtggaa aggagaagtc aatggacaag tggggctctt
1681 cccatccaat tatgtgaagc tgaccacaga catggaccca agccagcaat ggtgttcaga
1741 cttacatctc ttggatatgt tgaccccaac tgaaagaaag cgacaaggat acatccacga
1801 gctcattgtc accgaggaga actatgtgaa tgacctgcag ctggtcacag agatttttca
1861 aaaacccctg atggagtctg agctgctgac agaaaaagag gttgctatga tttttgtgaa
1921 ctggaaggag ctgattatgt gtaatatcaa actactaaaa gcgctgagag tccgcaagaa
1981 gatgtccggg gagaagatgc ctgtgaagat gattggagac atcctgagcg cacagctgcc
2041 gcacatgcag ccctacatcc gcttctgcag ccgccagctc aacggggctg ccctgatcca
2101 gcagaagacg gacgaggccc cagacttcaa ggagttcgtc aaaagattgg caatggatcc
2161 tcggtgtaaa gggatgccac tctctagttt tatactgaag cctatgcaac gggtaacaag
2221 atacccactg atcattaaaa atatcctgga aaacacccct gaaaaccacc cggaccacag
2281 ccacttgaag cacgccctgg agaaggcgga agagctctgt tcccaggtga acgaaggggt
2341 gcgggagaag gagaactctg accggctgga gtggatccag gcccacgtgc agtgtgaagg
2401 cctgtctgag caacttgtgt tcaattcagt gaccaattgc ttggggccgc gcaaatttct
2461 gcacagtggg aagctctaca aggccaagag caacaaggag ctgtatggct tccttttcaa
2521 cgacttcctc ctgctgactc agatcacgaa gcctttgggg tcttctggca ccgacaaagt
2581 cttcagcccc aaatcaaacc tgcagtataa aatgtataaa acacctattt tcctaaatga
2641 ggttctagta aaattaccca ccgacccttc tggagacgag cccatcttcc acatctccca
2701 cattgaccgc gtctatactc tccgagcaga aagcataaat gaaaggactg cctgggtgca
2761 gaaaatcaaa gctgcttctg aactctacat agagactgag aaaaagaagc gcgagaaagc
2821 gtacctggtc cgttcccaaa gggcaacagg cattggaagg ttgatggtga acgtggttga
2881 aggcatcgag ttgaaaccct gtcggtcaca tggaaagagc aacccgtact gtgaggtgac
2941 catgggttcc cagtgccaca tcaccaagac gatccaggac actctgaacc ccaagtggaa
3001 ttccaactgc cagttcttca tccgagacct ggagcaggaa gtcctctgca tcactgtgtt
3061 cgagagggac cagttctcac cagatgattt tttgggtcgg acggagatcc gcgtggcgga
3121 catcaagaaa gaccagggct ccaaaggtcc agttacgaag tgtcttctgc tgcacgaagt
3181 ccccacggga gagattgtgg tccgcttgga cctgcagttg tttgatgagc cgtgtacaga
3241 ctacaaagac catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga
3301 caagtaactc gagataatca acctctggat tacaaaattt gtgaaagatt gactggtatt
3361 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
3421 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttagttctt
3481 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
3541 ggcactgaca attccgtggt gtttatttgt gaaatttgtg atgctattgc tttatttgta
3601 accatctagc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
3661 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
3721 gatgtgggag gttttttaaa gatctggaga ccggtgtggc acgtgcggac cgagcggccg
3781 caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag
3841 gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag
3901 cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc
3961 ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa
4021 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccttagcgc
4081 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
4141 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
4201 aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
4261 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
4321 cactcaactc tatctcgggc tattcttttg atttataagg gattttgccg atttcggtct
4381 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
4441 cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
4501 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
4561 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
4621 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
4681 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
4741 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
4801 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
4861 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
4921 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
4981 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
5041 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
5101 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
5161 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
5221 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
5281 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
5341 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
5401 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
5461 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
5521 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
5581 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
5641 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
5701 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
5761 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
5821 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
5881 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
5941 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
6001 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
6061 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
6121 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
6181 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
6241 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
6301 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
6361 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
6421 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
6481 tacggttcct ggccttttgc tggccttttg ctcacatgt
//
