| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-GRIN2B-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001355 |
| 产品用途: | 3' AAVLINK plasmid for GRIN2B expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | GRIN2B |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-GRIN2B-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-GRIN2B-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-GRIN2B-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-GRIN2B-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-GRIN2B-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-GRIN2B-FLAG 6238 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6238)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 1, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6238
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
misc_feature 1547..2951
/label=GRIN2B
misc_feature 1582..1587
/label=SDS
misc_feature 2006..2011
/label=SDS
CDS 2958..3023
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3027..3458
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3171..3176
/label=SDS
repeat_region 3501..3641
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3501..3630
/label=AAV2 ITR
rep_origin 3716..4171
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4453..4557
/gene="bla"
/label=AmpR promoter
CDS 4558..5418
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5589..6177
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtcc atcagcaaga
1561 agcccctgga catcggcctc ccctcctcca agcacagcca gctcagtgac ctgtacggca
1621 aattctcctt caagagcgac cgctacagtg gccacgacga cttgatccgc tccgatgtct
1681 ctgacatctc aacccacacc gtcacctatg ggaacatcga gggcaatgcc gccaagaggc
1741 gtaagcagca atataaggac agcctgaaga agcggcctgc ctcggccaag tcccgcaggg
1801 agtttgacga gatcgagctg gcctaccgtc gccgaccgcc ccgctcccct gaccacaagc
1861 gctacttcag ggacaaggaa gggctacggg acttctacct ggaccagttc cgaacaaagg
1921 agaactcacc ccactgggag cacgtagacc tgaccgacat ctacaaggag cggagtgatg
1981 actttaagcg cgactccgtc agcggaggag ggccctgtac caacaggtct cacatcaagc
2041 acgggacggg cgacaaacac ggcgtggtca gcggggtacc tgcaccttgg gagaagaacc
2101 tgaccaacgt ggagtgggag gaccggtccg ggggcaactt ctgccgcagc tgtccctcca
2161 agctgcacaa ctactccacg acggtgacgg gtcagaactc gggcaggcag gcgtgcatcc
2221 ggtgtgaggc ttgcaagaaa gcaggcaacc tgtatgacat cagtgaggac aactccctgc
2281 aggaactgga ccagccggct gccccagtgg cggtgacgtc aaacgcctcc accactaagt
2341 accctcagag cccgactaat tccaaggccc agaagaagaa ccggaacaaa ctgcgccggc
2401 agcactccta cgacaccttc gtggacctgc agaaggaaga agccgccctg gccccgcgca
2461 gcgtaagcct gaaagacaag ggccgattca tggatgggag cccctacgcc cacatgtttg
2521 agatgtcagc tggcgagagc acctttgcca acaacaagtc ctcagtgccc actgccggac
2581 atcaccacca caacaacccc ggcggcgggt acatgctcag caagtcgctc taccctgacc
2641 gggtcacgca aaaccctttc atccccactt ttggggacga ccagtgcttg ctccatggca
2701 gcaaatccta cttcttcagg cagcccacgg tggcgggggc gtcgaaagcc aggccggact
2761 tccgggccct tgtcaccaac aagccggtgg tctcggccct tcatggggcc gtgccagccc
2821 gtttccagaa ggacatctgt atagggaacc agtccaaccc ctgtgtgcct aacaacaaaa
2881 accccagggc tttcaatggc tccagcaatg ggcatgttta tgagaaactt tctagtattg
2941 agtctgatgt ctgtacagac tacaaagacc atgacggtga ttataaagat catgacatcg
3001 actacaagga tgacgatgac aagtaactcg agataatcaa cctctggatt acaaaatttg
3061 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
3121 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
3181 taaatcctgg ttagttcttg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
3241 gacaggggct cggctgttgg gcactgacaa ttccgtggtg tttatttgtg aaatttgtga
3301 tgctattgct ttatttgtaa ccatctagct ttatttgtga aatttgtgat gctattgctt
3361 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
3421 tgtttcaggt tcagggggag atgtgggagg ttttttaaag atctggagac cggtgtggca
3481 cgtgcggacc gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg
3541 cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg
3601 ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt
3661 tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg
3721 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
3781 cttgccagcg ccttagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
3841 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
3901 ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg
3961 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
4021 ttgttccaaa ctggaacaac actcaactct atctcgggct attcttttga tttataaggg
4081 attttgccga tttcggtcta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
4141 aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct
4201 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
4261 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
4321 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
4381 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
4441 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
4501 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
4561 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
4621 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
4681 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
4741 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
4801 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
4861 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
4921 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
4981 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
5041 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
5101 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
5161 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
5221 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
5281 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
5341 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
5401 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
5461 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
5521 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
5581 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
5641 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
5701 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
5761 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
5821 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
5881 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
5941 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
6001 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
6061 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
6121 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
6181 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgt
//
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-GRIN2B-FLAG载体图谱质粒图谱pdf版和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-GRIN2B-FLAG载体序列质粒序列等相关资料下载
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-GRIN2B-FLAG质粒载体应用举例
- 上一篇：pAAVLINKv1-pCALM1-Cre-3'-GRIN2B-FLAG
- 下一篇：没有了
