| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-GRIN2A-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001448 |
| 产品用途: | 3' AAVLINK plasmid for GRIN2A expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | GRIN2A |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-GRIN2A-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-GRIN2A-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-GRIN2A-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-GRIN2A-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-GRIN2A-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-GRIN2A-FLAG 6631 bp DNA circular SYN 14-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6631)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 8, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6631
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3344
/codon_start=1
/label=GRIN2A
/translation="GHLQLHSWSAH*RKEEVSRLQSDGIPEQHVKTPPVSQKHFQHVQH
ELLKNGLTQKSC*LHPKRFPHHGHGFR*GEFDVLRQQVLSGEREHFWRQHERTPNICGQ
PAEG*PQ*LCIPGTTSSYSQ*VQP*HGGGGREHRIQSEL*TPAAVEEIRGFHTPGFTIP
ESSLPEG*GNSRE*DPLPKEP*VSSRRDGPL*HFRNVKSGHVPQGT*QQ*EPQNQGQL*
KVSGLQIPQGL**GRAHLPENQIKLP*RQDLHYRW*EGAWFPLRSTPVC*KCDPARERG
LPGPLPGSQ*KLPQGGLHAANEPEPLA**RGAFQQRPV*TLLQALHLERQGFPAQ*DQR
AIPAELHALQKLPFQHAHLFRPLHHEVPLQVRCLPADGEPL*HR*RPDASGDR*PSHRG
AGLPAGLGTEQCPSITKEQAKD*PSAFLR*HCRQT*GARP*QALPEHKPQGQGTASGGK
FLRQPV*CPLKQTLGEKKLPFPPRSGGQQEEQVSLARPHLR*PFPPLPQG*PTLGYWEM
PLGPLQTLVAIPGGE*QLSSVVLEVNGIVLFQGQSGPQ*CVYFGACYALCCK*E*YVLY
PQGFKFLQQ*TRVQENA*YRI*C"
CDS 3351..3416
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3420..3851
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3564..3569
/label=SDS
repeat_region 3894..4034
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3894..4023
/label=AAV2 ITR
rep_origin 4109..4564
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4846..4950
/gene="bla"
/label=AmpR promoter
CDS 4951..5811
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5982..6570
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggggc atctacagct
1561 gcattcatgg agtgcacatt gaagaaaaga agaagtctcc agacttcaat ctgacgggat
1621 cccagagcaa catgttaaaa ctcctccggt cagccaaaaa catttccagc atgtccaaca
1681 tgaactcctc aagaatggac tcacccaaaa gagctgctga cttcatccaa agaggttccc
1741 tcatcatgga catggtttca gataagggga atttgatgta ctcagacaac aggtcctttc
1801 aggggaaaga gagcattttt ggagacaaca tgaacgaact ccaaacattt gtggccaacc
1861 ggcagaagga taacctcaat aactatgtat tccagggaca acatcctctt actctcaatg
1921 agtccaaccc taacacggtg gaggtggccg tgagcacaga atccaaagcg aactctagac
1981 cccggcagct gtggaagaaa tccgtggatt ccatacgcca ggattcacta tcccagaatc
2041 cagtctccca gagggatgag gcaacagcag agaataggac ccactcccta aagagcccta
2101 ggtatcttcc agaagagatg gcccactctg acatttcaga aacgtcaaat cgggccacgt
2161 gccacaggga acctgacaac agtaagaacc acaaaaccaa ggacaacttt aaaaggtcag
2221 tggcctccaa ataccccaag gactgtagtg aggtcgagcg cacctacctg aaaaccaaat
2281 caagctcccc tagagacaag atctacacta tagatggtga gaaggagcct ggtttccact
2341 tagatccacc ccagtttgtt gaaaatgtga ccctgcccga gaacgtggac ttcccggacc
2401 cctaccagga tcccagtgaa aacttccgca agggggactc cacgctgcca atgaaccgga
2461 accccttgca taatgaagag gggctttcca acaacgacca gtataaactc tactccaagc
2521 acttcacctt gaaagacaag ggttccccgc acagtgagac cagcgagcga taccggcaga
2581 actccacgca ctgcagaagc tgcctttcca acatgcccac ctattcaggc cacttcacca
2641 tgaggtcccc cttcaagtgc gatgcctgcc tgcggatggg gaacctctat gacatcgatg
2701 aagaccagat gcttcaggag acaggtaacc cagccaccgg ggagcaggtc taccagcagg
2761 actgggcaca gaacaatgcc cttcaattac aaaagaacaa gctaaggatt agccgtcagc
2821 attcctacga taacattgtc gacaaaccta gggagctaga ccttagcagg ccctcccgga
2881 gcataagcct caaggacagg gaacggcttc tggagggaaa tttttacggc agcctgttta
2941 gtgtcccctc aagcaaactc tcggggaaaa aaagctccct tttcccccaa ggtctggagg
3001 acagcaagag gagcaagtct ctcttgccag accacacctc cgataaccct ttcctccact
3061 cccacaggga tgaccaacgc ttggttattg ggagatgccc ctcggaccct tacaaacact
3121 cgttgccatc ccaggcggtg aatgacagct atcttcggtc gtccttgagg tcaacggcat
3181 cgtactgttc cagggacagt cggggccaca atgatgtgta tatttcggag catgttatgc
3241 cttatgctgc aaataagaat aatatgtact ctacccccag ggttttaaat tcctgcagca
3301 atagacgcgt gtacaagaaa atgcctagta tcgaatctga tgtttgtaca gactacaaag
3361 accatgacgg tgattataaa gatcatgaca tcgactacaa ggatgacgat gacaagtaac
3421 tcgagataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
3481 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
3541 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttagttc ttgccacggc
3601 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
3661 caattccgtg gtgtttattt gtgaaatttg tgatgctatt gctttatttg taaccatcta
3721 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
3781 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gagatgtggg
3841 aggtttttta aagatctgga gaccggtgtg gcacgtgcgg accgagcggc cgcaggaacc
3901 cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg
3961 accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg
4021 cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
4081 acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg
4141 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccttagc gcccgctcct
4201 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
4261 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
4321 gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
4381 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
4441 tctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggt ctattggtta
4501 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca
4561 attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
4621 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
4681 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
4741 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
4801 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
4861 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
4921 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
4981 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
5041 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
5101 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
5161 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
5221 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
5281 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
5341 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
5401 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
5461 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
5521 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
5581 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
5641 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
5701 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
5761 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
5821 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
5881 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
5941 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
6001 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
6061 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
6121 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
6181 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
6241 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
6301 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
6361 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
6421 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
6481 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
6541 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
6601 ctggcctttt gctggccttt tgctcacatg t
//
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-GRIN2A-FLAG载体图谱质粒图谱pdf版和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-GRIN2A-FLAG载体序列质粒序列等相关资料下载
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-GRIN2A-FLAG质粒载体应用举例
- 上一篇：pAAVLINKv1-pCALM1-Cre-3'-GRIN2A-FLAG
- 下一篇：没有了
