| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CUX2-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001484 |
| 产品用途: | 3' AAVLINK plasmid for CUX2 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | CUX2 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CUX2-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CUX2-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CUX2-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CUX2-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CUX2-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CUX2-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CUX2-FLAG 6770 bp DNA circular SYN 14-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6770)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 12, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6770
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3483
/codon_start=1
/label=CUX2
/translation="GRRTNPPGRASSRLRARRPRRARGCPTTRPTCRAP*SPPCRR*PP
SSTSCTCTVR*TRWSSPARSRRSWPRTASARGSSGRRCWACHRAA*ATCCPGRSHGAS*
RRRGGSPSSACSCGSLTSSARQWASSLVPPRPVPQNQGPHHPHPPAPQSLRRAPRSR*A
CPWRAARRTSSQRAAPAPR*AGRCTQAARPQGASRRSWPCPPSWTRTPSPRG*RRSSQT
TI*GSGCLGKASWV*HRAPCLTCCPGPNPGTS*A*RGGSLLSACSCGSMTPITWRS*GI
*RSWRRKPT*NVAMASSAPAQTVSPRPPAQSAPAPACSPRT*ASCRSRSPGWCWHPRRR
RHCGRPISWNPTPRSRPSSSSPSSSTSRPTPSSTGSTTTGPGCAGRCWWRGPRMSQTLI
QAGVLESYRQATPTQTPPRRALTLRLRTRSQP*RNWSFRRALRRTAHP*PPRTRPK*GS
SRNRWRRMLRKRQAASPRTQGSWTKAKVPPKRSIPTLRVMMDSQKWLPGPSFQVDPPQT
VPHFIPNRRVRPGSDFTRTL*VLSQPQSPHAAAWRCH*TRPRPPPHQAS*CLCHLSPPP
QLPSPHPHLAPPLPKCRVPAPLLTWLEPCTPVPR*TPTCSGGMRRWPI*TTSFTD*SGL
PIGRRPWSGS"
misc_feature 2195..2200
/label=SDS
misc_feature 2517..2522
/label=SDS
misc_feature 2564..2569
/label=SDS
misc_feature 2858..2863
/label=SDS
misc_feature 2936..2941
/label=SDS
misc_feature 3069..3074
/label=SDS
misc_feature 3227..3232
/label=SDS
misc_feature 3266..3271
/label=SDS
misc_feature 3269..3274
/label=SDS
misc_feature 3461..3466
/label=SDS
CDS 3490..3555
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3559..3990
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3703..3708
/label=SDS
repeat_region 4033..4173
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4033..4162
/label=AAV2 ITR
rep_origin 4248..4703
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4985..5089
/gene="bla"
/label=AmpR promoter
CDS 5090..5950
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6121..6709
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggggc ggaggacgaa
1561 ccccccagga cgggcgagct caaggctgag ggcgcgacgg ccgaggcggg cgcgcggctg
1621 ccctactacc cggcctacgt gccgcgcacc ctgaagccca ccgtgccgcc gctgaccccc
1681 gagcagtacg agctgtacat gtaccgtgag gtagacacgc tggagctcac ccgccaggtc
1741 aaggagaagc tggccaagaa cggcatctgc cagaggatct tcggggagaa ggtgctgggc
1801 ctgtcacagg gcagcgtgag cgacatgctg tcccggccga agccatggag caagctgacg
1861 cagaaggggc gggagccctt catccgcatg cagctgtggc tctctgacca gctcggccag
1921 gcagtgggcc agcagcctgg tgcctcccag gccagtccca cagaaccaag gtcctcacca
1981 tccccacccc ccagccccac agagcctgag aagagctccc aggagccgtt gagcctgtcc
2041 ctggagagca gcaaggagaa ccagcagcca gagggccgct ccagctcctc gttgagcggg
2101 aagatgtact caggcagcca ggccccaggg ggcatccagg agatcgtggc catgtccccc
2161 gagctggaca cgtactccat caccaagagg gtgaaggagg tcctcacaga caacaatcta
2221 gggcagcggc tgtttgggga aagcatcctg ggtctgacac agggctccgt gtctgacctg
2281 ctgtcccggc ccaaaccctg gcacaagctg agcctgaagg ggcgggagcc ttttgtccgc
2341 atgcagctgt ggctcaatga cccccataac gtggagaagc tgagggatat gaagaagctg
2401 gagaagaaag cctacctgaa acgtcgctat ggcctcatca gcaccggctc agacagtgag
2461 tccccggcca cccgctcaga gtgccccagc ccctgcctgc agccccagga cctgagcctc
2521 ctgcagatca agaagccccg ggtggtgctg gcacccgagg agaaggaggc actgcggaag
2581 gcctatcagc tggaacccta cccctcgcag cagaccatcg agctcctctc cttccagctc
2641 aacctcaaga ccaacaccgt catcaactgg ttccacaact acaggtcccg gatgcgccgg
2701 gagatgttgg tggaggggac ccaggatgag ccagaccttg atccaagcgg gggtcctgga
2761 atcctaccgc caggccactc ccacccagac cccaccccgc agagccctga ctctgagact
2821 gaggaccaga agccaaccgt gaaggaactg gagcttcagg agggccctga ggagaacagc
2881 acacccctga ccacccagga caaggcccaa gtgaggatca agcaggaaca gatggaggag
2941 gatgctgagg aagaggcagg cagccagccc caggactcag gggagctgga caaaggccaa
3001 ggtcccccca aagaggagca tcccgaccct ccgggtaatg atggactccc aaaagtggct
3061 cccgggcccc tccttccagg tggatccacc ccagactgtc cctcacttca tccccaacag
3121 gagagtgagg ccggggagcg acttcacccg gaccctttaa gttttaagtc agcctcagag
3181 tcctcacgct gcagcctgga ggtgtcactg aactcgccct cggccgcctc ctcaccaggc
3241 ctcatgatgt ctgtgtcacc tgtcccctcc tcctcagctc ccatctcccc atccccacct
3301 ggcgcccccc ctgccaaagt gccgagtgcc agccccactg ctgacatggc tggagccttg
3361 caccccagtg ccaaggtgaa ccccaacttg cagcggcggc atgagaagat ggccaatctg
3421 aacaacatca tttaccgact agagcgggct gccaatcggg aggaggccct ggagtgggag
3481 ttctgtacag actacaaaga ccatgacggt gattataaag atcatgacat cgactacaag
3541 gatgacgatg acaagtaact cgagataatc aacctctgga ttacaaaatt tgtgaaagat
3601 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
3661 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
3721 ggttagttct tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
3781 ctcggctgtt gggcactgac aattccgtgg tgtttatttg tgaaatttgt gatgctattg
3841 ctttatttgt aaccatctag ctttatttgt gaaatttgtg atgctattgc tttatttgta
3901 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
3961 gttcaggggg agatgtggga ggttttttaa agatctggag accggtgtgg cacgtgcgga
4021 ccgagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct
4081 cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct
4141 cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat tttctcctta
4201 cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag
4261 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
4321 cgccttagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
4381 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
4441 cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata
4501 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
4561 aactggaaca acactcaact ctatctcggg ctattctttt gatttataag ggattttgcc
4621 gatttcggtc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
4681 caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc
4741 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
4801 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
4861 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
4921 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
4981 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
5041 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
5101 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
5161 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
5221 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
5281 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
5341 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
5401 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
5461 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
5521 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
5581 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
5641 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
5701 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
5761 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
5821 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
5881 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
5941 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
6001 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
6061 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
6121 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
6181 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
6241 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
6301 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
6361 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
6421 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
6481 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
6541 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
6601 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
6661 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
6721 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
//
