pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CUX1-FLAG

LOCUS       pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CUX1-FLAG               7388 bp DNA     circular SYN 14-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7388)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7388
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     misc_feature    145..225
                     /label=SCP1
     CDS             237..1265
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1281..1370
                     /codon_start=1
                     /label=UDeg3a
                     /translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
     misc_feature    1295..1300
                     /label=SDS
     misc_feature    1298..1303
                     /label=SDS
     intron          1374..1414
                     /label=Splicing Donor
     protein_bind    1421..1454
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1455..1546
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1547..4101
                     /codon_start=1
                     /label=CUX1
                     /translation="VTSPPGSEPRRLALMKPSSPS*SKPRGSSKCRKLQSRPSLPPHPA
                     AGTLMTPSAPSCSKPAGRWRPSRLPSTLP*SRHHCPRVTSPSSPPSFCPPRPCPPCPAT
                     HLSPSP*RSPPQLLRPVPLLCRTPRPSKRRPRTPPGWTPREQPIVHKGS*DR*KMRWAA
                     AVPGRTTGGARCSRREEMPPPPRRPRPKKRAAGKRRAAVAAEVAASLGPSAVSSRDPRR
                     QSTGRSGPALSPHTPRAQS*V*PGPAAARHHRTAPCHPPRSCPCPSPPSPRSPR*PPSS
                     TRSTCTRRWTPSSSPGRLRKSWPRTASARESSGRRCWACPRAASATCCPDRSHGAS*RR
                     KAENPSSGCSSG*TAS*ARVFYPSRASSKGQSSTP*HRSRTRCSRAV*AQKALQRPPPA
                     AALPLSPR*VPVSR*RA*PSWSSSPVPPSRRARTASHQSPVTRQHPTPSPQPRCLSPDT
                     RPSASKN**PCPRSWTPTA*PSG*RRC*RTTTSASAYLGRPS*GSPKALSLTSLPAPNP
                     GISSV*KDESPSSGCSCG*TTPTMWRS*WT*NGWRRKPT*SGGTAQSVTASPANRPLSA
                     PSTARAPAPSPSTS*RNPGWCWLRRRRRR*NERISKSHTRHQKPSKTSPPSST*KPAPS
                     STGSTTTGLGSAENCSLRKFRPGVRARRAPATHPRPAAAGRRPARRATAATAWRPLRAQ
                     AAPTPRSPSLRERPSGRRCRGRRSRRSRRPRGPRARTTPATTTTREAPWKARGPCPAPP
                     PRPPPPRPRPPRTPLPQPPPRRGRAPRPRAPRRRPATAAAAAPPAGPARCRAFSASPRP
                     RAPGTRATTPCARRRPRT*TASSTAWRRPPAGRNLSNGS"
     misc_feature    1943..1948
                     /label=SDS
     misc_feature    2093..2098
                     /label=SDS
     misc_feature    2102..2107
                     /label=SDS
     misc_feature    2408..2413
                     /label=SDS
     misc_feature    2993..2998
                     /label=SDS
     misc_feature    3075..3080
                     /label=SDS
     misc_feature    3353..3358
                     /label=SDS
     misc_feature    3677..3682
                     /label=SDS
     CDS             4108..4173
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      4177..4608
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    4321..4326
                     /label=SDS
     repeat_region   4651..4791
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4651..4780
                     /label=AAV2 ITR
     rep_origin      4866..5321
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5603..5707
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5708..6568
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6739..7327
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
      181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
      241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
      301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
      361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
      421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
      481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
      541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
      601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
      661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
      721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
      781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
      841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
      901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
      961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
     1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
     1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
     1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
     1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
     1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
     1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
     1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
     1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtaa catcaccacc
     1561 cggatccgag cctcggagac tggctctgat gaagccatca agtccatcct agagcaagcc
     1621 aagagggagc tccaagtgca gaaaactgca gagccggccc agccttcctc cgcatccggc
     1681 agcgggaact ctgatgacgc catccgctcc atcctgcagc aagcccgccg ggagatggag
     1741 gcccagcagg ctgccctcga ccctgcctta aagcaggcac cactgtccca gagtgacatc
     1801 accatcctca cccccaagct tctgtccacc tcgcccatgc ccaccgtgtc cagctaccca
     1861 cctctcgcca tctccctgaa gaagccctcc gcagctcctg aggccggtgc ctctgctctg
     1921 ccgaaccccc cggccctcaa aaaggaggcc caggacgccc ccgggctgga cccccaggga
     1981 gcagccgatt gtgcacaagg ggtcctgaga caggtgaaaa atgaggtggg ccgcagcggt
     2041 gcctggaagg accactggtg gagcgcggtg cagccggaga gaagaaatgc cgcctcctcc
     2101 gaggaggcca aggccgaaga aacgggcggc gggaaagaga agggcagcgg tggcagcgga
     2161 ggtggcagcc agcctcgggc cgagcgcagt cagctccagg gaccctcgtc gtcagagtac
     2221 tggaaggagt ggcccagcgc tgagtcccca tactcccaga gctcagagct gagtctgacc
     2281 ggggccagcc gcagcgagac accacagaac agccccctgc catcctcccc gatcgtgccc
     2341 atgtccaagc ccaccaagcc ctcggtcccc ccgctgaccc ccgagcagta cgaggtctac
     2401 atgtaccagg aggtggacac catcgagctc acccggcagg ttaaggaaaa gctggccaag
     2461 aacggcatct gccagagaat cttcggggag aaggtgctgg gcctgtccca gggcagcgtc
     2521 agcgacatgc tgtcccgacc gaagccatgg agcaagctga cgcagaaagg ccgagaaccc
     2581 ttcatccgga tgcagctctg gctgaacggc gagctaggcc agggtgttct acccgtccag
     2641 ggccagcagc aagggccagt cctccactcc gtgacatcgc tccaggaccc gctgcagcag
     2701 ggctgtgtga gctcagaaag cactccaaag acctccgcca gctgcagccc tgcccctgag
     2761 tccccgatga gttccagtga gtcggtgaag agcctgaccg agctggtcca gcagccctgt
     2821 ccccccatcg aggcgagcaa ggacagcaag ccaccagagc ccagtgaccc gccagcatcc
     2881 gactcccagc ccacaacccc gctgcctctc tccggacact cggccctcag catccaagaa
     2941 ttagtagcca tgtccccgga gctggacacc tacggcataa ccaagcgggt gaaggaggtg
     3001 ctgacggaca acaacctcgg ccagcgctta tttggggaga ccatcttagg gctcacccaa
     3061 ggctctgtct ctgacctcct tgcccgcccc aaaccctggc ataagctcag tctgaaagga
     3121 cgagagccct tcgtccggat gcagctgtgg ctgaacgacc ccaacaatgt ggagaagctg
     3181 atggacatga aacggatgga gaagaaagcc tacatgaagc ggcggcacag ctcagtcagt
     3241 gacagccagc cctgcgaacc gccctctgtc ggcaccgagt acagccaggg cgccagcccc
     3301 cagccccagc accagctgaa gaaaccccgg gtggtgctgg ctccggagga gaaggaggcg
     3361 ctgaaacgag cgtatcagca aaagccatac ccgtcaccaa aaaccatcga agacctcgcc
     3421 acccagctca acctgaaaac cagcaccgtc atcaactggt tccacaacta caggtctcgg
     3481 atccgcagag aactgttcat tgaggaaatt caggccggga gtcagggcca ggcgggcgcc
     3541 agcgactcac cctcggcccg cagcggccgg gcggcgccca gctcggaggg cgacagctgc
     3601 gacggcgtgg aggccactga gggcccaggc agcgccgaca ccgaggagcc caagtctcag
     3661 ggagaggccg agcgggagga ggtgccgcgg ccggcggagc agacggagcc gccgccctcg
     3721 gggaccccgg gcccggacga cgcccgcgac gacgaccacg agggaggccc cgtggaaggc
     3781 ccggggcccc tgcccagccc cgcctccgcg accgccaccg ccgcgcccgc ggcccccgag
     3841 gacgccgcta cctcagccgc cgccgcgccg ggggagggcc ccgcggcccc gagctccgcg
     3901 ccgccgccca gcaacagcag cagcagcagc gccccccgca ggcccagctc gctgcagagc
     3961 cttttcggcc tccccgaggc cgcgggcgcc cgggactcgc gcgacaaccc cctgcgcaag
     4021 aagaaggccg cgaacttgaa cagcatcatc caccgcctgg agaaggccgc cagccgggag
     4081 gaacctatcg aatgggagtt ctgtacagac tacaaagacc atgacggtga ttataaagat
     4141 catgacatcg actacaagga tgacgatgac aagtaactcg agataatcaa cctctggatt
     4201 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     4261 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     4321 cctccttgta taaatcctgg ttagttcttg ccacggcgga actcatcgcc gcctgccttg
     4381 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg tttatttgtg
     4441 aaatttgtga tgctattgct ttatttgtaa ccatctagct ttatttgtga aatttgtgat
     4501 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     4561 attcatttta tgtttcaggt tcagggggag atgtgggagg ttttttaaag atctggagac
     4621 cggtgtggca cgtgcggacc gagcggccgc aggaacccct agtgatggag ttggccactc
     4681 cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg
     4741 gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga
     4801 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc
     4861 atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     4921 gaccgctaca cttgccagcg ccttagcgcc cgctcctttc gctttcttcc cttcctttct
     4981 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     5041 atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag
     5101 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     5161 tagtggactc ttgttccaaa ctggaacaac actcaactct atctcgggct attcttttga
     5221 tttataaggg attttgccga tttcggtcta ttggttaaaa aatgagctga tttaacaaaa
     5281 atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac
     5341 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
     5401 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     5461 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
     5521 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     5581 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     5641 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     5701 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     5761 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     5821 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     5881 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     5941 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     6001 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     6061 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     6121 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     6181 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     6241 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     6301 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     6361 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     6421 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     6481 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     6541 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     6601 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     6661 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     6721 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     6781 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     6841 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     6901 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     6961 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     7021 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     7081 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     7141 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     7201 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     7261 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     7321 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     7381 tcacatgt
//