| 质粒名称: |
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CLASP1-FLAG
|
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001496 |
| 产品用途: | 3' AAVLINK plasmid for CLASP1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: |
SCP1
|
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | CLASP1 |
| 物种来源: |
H.sapiens
|
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CLASP1-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CLASP1-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CLASP1-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CLASP1-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CLASP1-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CLASP1-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CLASP1-FLAG 6824 bp DNA circular SYN 14-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6824)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 12, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6824
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3537
/codon_start=1
/label=CLASP1
/translation="GFWACRTY*RAKEH*VELN*KGCVRSSLGCLLTLIAREFSVCFWR
LLWIL**FIRMIYKTGFLFFSHNYLRKWEQIYLDLCKQKFKRL*MSQGTPFHLINNLTF
**DLLWIKLKLQTSRSKLQS*NTLSLWPDRWIQQIL*TLVRQGLLFLES*PGQQNQRVQ
T*ERQHRLC*SLCLN*ILLNLPCYLVPCQKHSRMVPPNSCTTTSRIPVTPVWALQAIRL
AGRPPDTPAAGPAP*PHPPTVPMGVCLQVGYGVGVPTG*RSTHLPFLSLTPSPPLPPTR
LSGALTLPACWTMIQRT*TLKKSIVLYVELQKPLKSLVFEAKKI*MSQLNEMAKRSVIL
CPAMGALPPLPLRAGGVVK*KEAGQLWITRPHYSTPSLRAPSRGRGRETTTRTPTQMPS
TPTTRPP*KRLCSMTTWSSFETCPSTILTWWLTF*KSCPTTMSEWRNGREPCWSCSRSR
GKTALVSGRSTSRPFCSCCWRPLETKTIQFEHWR*EF*GKF*EINQQDLKTTPS*RL*R
LWKPTKTPIRRW*ERLRRLRPHWPVPSTRSSASRCSAPSSRRPTTPSTLLPSRCRPKSS
RGSQRSHCCSSLSTSSQACCRVMTTPKVVCVRPACFA*WQFIP*SEKT*NLTLHSSQGA
R*SY*TYT*RGPRPPTATAAPPPMSPRT"
misc_feature 2628..2633
/label=SDS
RBS 3123..3131
/label=Shine-Dalgarno sequence
/note="full consensus sequence for ribosome-binding sites
upstream of start codons in E. coli; complementary to a
region in the 3' end of the 16S rRNA (Chen et al., 1994)"
misc_feature 3125..3130
/label=SDS
misc_feature 3146..3151
/label=SDS
misc_feature 3512..3517
/label=SDS
CDS 3544..3609
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3613..4044
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3757..3762
/label=SDS
repeat_region 4087..4227
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4087..4216
/label=AAV2 ITR
rep_origin 4302..4757
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5039..5143
/gene="bla"
/label=AmpR promoter
CDS 5144..6004
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6175..6763
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacagggct tctgggcctg
1561 cagaacttac tgaagagcca aagaacactg agtcgagttg aactgaaaag gttgtgtgag
1621 atcttcactc ggatgtttgc tgaccctcat agcaagagag ttttcagtat gtttttggag
1681 actcttgtgg attttataat aattcataag gatgatttac aagactggct ttttgttctt
1741 ctcacacaat tacttaagaa aatgggagca gatttacttg gatctgtgca agcaaaagtt
1801 caaaaggctc tagatgtcac aagggactcc tttccatttg atcaacaatt taacattttg
1861 atgagattta ttgtggatca aactcaaact ccaaacctca aggtcaaagt tgcaatcctg
1921 aaatacattg agtctctggc cagacagatg gatccaacag attttgtaaa ctctagtgag
1981 acaaggcttg ctgtttctag aatcataacc tggacaacag aaccaaagag ttcagacgtg
2041 agaaaggcag cacagattgt gctaatctct ctgtttgaat tgaatactcc tgaatttacc
2101 atgttacttg gtgccttgcc aaaaacattc caggatggtg ccaccaaact cctgcacaac
2161 cacctcaaga attccagtaa caccagtgtg ggctctccaa gcaatacgat tggccggacg
2221 ccctcccgac acaccagcag caggaccagc cccctgacct cacccaccaa ctgttcccat
2281 gggggtctgt ctccaagtcg gttatggggt tggagtgccg acgggttagc gaagcaccca
2341 cctccctttt ctcagcctaa ctccatcccc accgctccct cccacaaggc tctcaggcgc
2401 tcttactctc ccagcatgct ggactatgat acagagaacc tgaactctga agaaatctat
2461 agttctctac gtggagttac agaagccatt gaaaagttta gttttcgaag ccaagaagat
2521 ctgaatgagc caattaaacg agatggcaaa aaggagtgtg atattgtgtc ccgcgatggg
2581 ggcgctgcct cccctgccac tgagggccgg gggggtagtg aagtagaagg aggccggaca
2641 gctctggata acaagacctc actactcaac acccagcctc cgcgcgcctt cccggggccg
2701 cgggcgcgag actacaaccc gtacccctac tcagatgcca tcaacaccta cgacaagacc
2761 gccctgaaag aggctgtgtt cgatgacgac atggagcagc ttcgagacgt gcccatcgac
2821 cattctgacc tggtggctga ccttctgaaa gagctgtcca accacaatga gcgagtggag
2881 gaacggaagg gagccctgct ggagctgctc aagatcacgc gggaagacag ccttggtgtc
2941 tgggaggagc acttcaagac cattctgctc ctgctgctgg agacccttgg agacaaagac
3001 cattcaattc gagcactggc gttaagagtt ttgagggaaa ttctgagaaa tcaaccagca
3061 agatttaaaa actacgccga gctgacgatt atgaagactc tggaagccca caaagactcc
3121 cataaggagg tggtgagagc ggctgaggag gctgcgtcca cactggccag ttccatccac
3181 ccggagcagt gcatcaaggt gctctgcccc atcatccaga cggccgacta ccccatcaac
3241 cttgctgcca tcaagatgca gaccaaagtc gtcgagagga tcgcaaagga gtcattgctg
3301 cagctccttg tcgacatcat cccaggcttg ctgcagggtt atgacaacac cgaaagtagt
3361 gtgcgtaagg ccagcgtgtt ttgcttagtg gcaatttatt ccgtaatcgg agaagacctg
3421 aaacctcacc ttgcacagct cacagggagc aagatgaagc tactaaactt atacataaag
3481 agggcccaga ccaccaacag caacagcagc tcctcctccg atgtctccac gcacagctgt
3541 acagactaca aagaccatga cggtgattat aaagatcatg acatcgacta caaggatgac
3601 gatgacaagt aactcgagat aatcaacctc tggattacaa aatttgtgaa agattgactg
3661 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
3721 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttag
3781 ttcttgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
3841 tgttgggcac tgacaattcc gtggtgttta tttgtgaaat ttgtgatgct attgctttat
3901 ttgtaaccat ctagctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
3961 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
4021 ggggagatgt gggaggtttt ttaaagatct ggagaccggt gtggcacgtg cggaccgagc
4081 ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca
4141 ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga
4201 gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc
4261 tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc
4321 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgcctt
4381 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
4441 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
4501 ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt
4561 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
4621 aacaacactc aactctatct cgggctattc ttttgattta taagggattt tgccgatttc
4681 ggtctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
4741 attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
4801 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
4861 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
4921 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
4981 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
5041 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
5101 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
5161 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
5221 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
5281 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
5341 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
5401 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
5461 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
5521 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
5581 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
5641 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
5701 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
5761 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
5821 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
5881 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
5941 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
6001 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
6061 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
6121 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
6181 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
6241 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
6301 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
6361 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
6421 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
6481 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
6541 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
6601 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
6661 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
6721 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
6781 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgt
//
